Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,287,116 G→T 5.5% intergenic (+91/‑310) agaI → / → yraH galactosamine‑6‑phosphate isomerase/putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,287,1160GT 5.5% 263.8 / 5.3 103intergenic (+91/‑310)agaI/yraHgalactosamine‑6‑phosphate isomerase/putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (49/48);  new base T (4/2);  total (53/50)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 104 total aligned reads displayed.

ATCCCGGCTTCATTTTTATGGCTGCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACATAGAAAATTGATAT  >  NC_000913/3286969‑3287264
                                                                                                                                                   |                                                                                                                                                    
aTCCCGGCTTCATTTTTATGGCTGCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGcc                                                                                                                                                    >  2:1232013/1‑150 (MQ=255)
  cccGGCTTCATTTTTATGGCTGCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACTcctt                                                                                                                                                  <  2:1197709/150‑3 (MQ=255)
   ccGGCTTCATTTTTATGGCTGCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGTTTGAACATAACGCCTAt                                                                                                                                                 >  1:152275/1‑150 (MQ=255)
       cTTCATTTTTATGGCTGCACAGTAACTTTATTTGTTTAATTAACTCCTAACCACATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTATCAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCa                                                                                                                                             <  2:144641/150‑1 (MQ=255)
          cATTTTTATGGCTGCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCaaaa                                                                                                                                          >  1:1126020/1‑150 (MQ=255)
           aTTTTTATGGCTGCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAg                                                                                                                                         <  2:1250919/150‑1 (MQ=255)
               ttATGGCTGCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGt                                                                                                                                     <  1:444563/150‑1 (MQ=255)
                 aTGGCTGCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTa                                                                                                                                   >  2:1317021/1‑150 (MQ=255)
                 aTGGCTGCACAGTAACTTTAATTGATTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTa                                                                                                                                   <  2:196384/150‑1 (MQ=255)
                   ggCTGCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATg                                                                                                                                 >  2:18359/1‑150 (MQ=255)
                    gCTGCACAGTAACTTTATTTGTTTAATTAACCCCTAACCAGATGTAAAATTTGCTCTAAATGTTTAAATTAACTTATGTAACAGTCCCGCATTATATTAAATAACATTTGACTGGGTTTAACAGAACGCGGCTAGCAAAAGGAGTTATGt                                                                                                                                >  1:666659/1‑150 (MQ=255)
                    gCTGCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCAGATAGCAAAAGGAGTTATGt                                                                                                                                >  1:780756/1‑150 (MQ=255)
                       gCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTca                                                                                                                             >  1:1245126/1‑150 (MQ=255)
                       gCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGAAAAAGGAGTTATGTTca                                                                                                                             >  2:45956/1‑150 (MQ=255)
                        cacaGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTcaa                                                                                                                            <  2:458735/150‑1 (MQ=255)
                          caGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACTCCTTTTGCTATCGGCGTTATGTTcaacc                                                                                                                          <  2:604223/150‑2 (MQ=38)
                          caGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTcaaca                                                                                                                          >  1:439484/1‑150 (MQ=255)
                          caGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTcaaca                                                                                                                          >  2:1030046/1‑150 (MQ=255)
                            gTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACaat                                                                                                                        >  2:1225575/1‑150 (MQ=255)
                            gTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACaat                                                                                                                        >  2:1167647/1‑150 (MQ=255)
                              aaCTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACaattt                                                                                                                      <  1:890740/150‑1 (MQ=255)
                               aCTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACTCCGATATCAAAAGGAGTTATGTAAAACaattta                                                                                                                     >  1:1165453/1‑150 (MQ=255)
                                 tttATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACaatttaat                                                                                                                   >  1:581177/1‑150 (MQ=255)
                                  ttATTTGTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATTTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACaatttaattt                                                                                                                 <  2:832202/150‑1 (MQ=255)
                                   tATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATTTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGCTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACaatttaattt                                                                                                                 <  1:646289/150‑1 (MQ=255)
                                      ttGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAg                                                                                                              <  2:1118413/150‑1 (MQ=255)
                                      ttGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAg                                                                                                              <  2:506209/150‑1 (MQ=255)
                                       tGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGa                                                                                                             <  2:280600/150‑1 (MQ=255)
                                       tGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGa                                                                                                             <  1:1188883/150‑1 (MQ=255)
                                        gTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATGACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGat                                                                                                            <  2:242064/150‑1 (MQ=255)
                                        gTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGat                                                                                                            <  1:1232013/150‑1 (MQ=255)
                                        gTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATAAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGat                                                                                                            >  1:627695/1‑150 (MQ=255)
                                         ctaacttaaCACCTAACCAGATGAAAAATTTGCTAAAACTGTTTACCTTAAATTATGTAACAGTCACGCATTATATTAACTAACAATTGACTGGGTTGAACATAACGCCGATAGCAAAACGAGTTATGTTCAACAATTTAATTTTAGata                                                                                                           <  2:192868/145‑1 (MQ=255)
                                           taattaaCACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGAtata                                                                                                         >  1:1144273/1‑150 (MQ=255)
                                           taattaaCACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGAAGATAGAAAAAGGAGTTATGTTCAACAATTTAATTTTAGAtata                                                                                                         >  1:757538/1‑150 (MQ=255)
                                                    ccTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACt                                                                                                >  1:581710/1‑150 (MQ=255)
                                                         ccAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTc                                                                                           >  2:193739/1‑150 (MQ=255)
                                                          cAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCt                                                                                          >  1:800219/1‑150 (MQ=255)
                                                            gATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTAt                                                                                        >  1:1313418/1‑150 (MQ=255)
                                                              tGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAAATTAATTTTAGATATACATATAACTTGTTCTATta                                                                                      >  1:877899/1‑150 (MQ=255)
                                                               taaaaaTTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATtaa                                                                                     <  1:699560/149‑1 (MQ=255)
                                                               gAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATtaa                                                                                     <  1:884565/150‑1 (MQ=255)
                                                                aaaaaTTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATtaat                                                                                    >  2:120931/1‑150 (MQ=255)
                                                                 aaaaTTTGCTCAAAATGTTTAAATTAAGTTATGTAACAGTCACGCATTATATTAAATAACTTTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATtaata                                                                                   <  2:343152/150‑1 (MQ=255)
                                                                     tttGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATc                                                                               <  1:1283995/150‑1 (MQ=255)
                                                                     tttGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATc                                                                               <  1:383907/150‑1 (MQ=255)
                                                                       tGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAAAATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCAc                                                                             >  1:153913/1‑150 (MQ=255)
                                                                         cTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCt                                                                           >  2:335415/1‑150 (MQ=255)
                                                                           cAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATTTACATATAACTTGTTCTATTAATAAATCACCTCg                                                                         <  1:968448/150‑1 (MQ=255)
                                                                           cAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCg                                                                         >  1:945871/1‑150 (MQ=255)
                                                                            aaaaTGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGt                                                                        >  1:291461/1‑150 (MQ=255)
                                                                             aaaTGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTAATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCTTa                                                                       >  1:81969/1‑150 (MQ=255)
                                                                                 gTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTACATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCt                                                                   >  2:1283906/1‑150 (MQ=255)
                                                                                    tAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGaa                                                                >  1:424807/1‑149 (MQ=255)
                                                                                     aaaTTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACt                                                               <  1:308047/150‑1 (MQ=255)
                                                                                     aaaTTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACt                                                               <  2:934085/150‑1 (MQ=255)
                                                                                      aaTTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTc                                                              >  2:416159/1‑150 (MQ=255)
                                                                                       aTTAACTTATGTAACAGTCACGAATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACATGTTCTATTAATAAATCACCTCGTATTCTGACTCg                                                             <  2:955283/150‑1 (MQ=255)
                                                                                          aaCTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGccc                                                          >  2:351128/1‑150 (MQ=255)
                                                                                          aaCTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGccc                                                          >  2:1083517/1‑150 (MQ=255)
                                                                                               aTGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTgg                                                     <  2:715249/150‑1 (MQ=255)
                                                                                                   aaCAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCgca                                                 <  2:1164204/150‑1 (MQ=255)
                                                                                                        tCACTCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCaaa                                            <  1:798977/150‑1 (MQ=255)
                                                                                                         cACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAg                                           >  2:1024982/1‑150 (MQ=255)
                                                                                                         cACGCATTATATAAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAg                                           <  2:606155/150‑1 (MQ=255)
                                                                                                             cATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAg                                       >  1:842740/1‑150 (MQ=255)
                                                                                                              aTTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAgg                                      <  1:1243205/150‑1 (MQ=255)
                                                                                                              aTTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAgg                                      <  1:265279/150‑1 (MQ=255)
                                                                                                               ttATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGc                                     <  1:261367/150‑1 (MQ=255)
                                                                                                                       aaTAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCATCAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATAc                             >  1:548682/1‑150 (MQ=255)
                                                                                                                         tAACCTTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATActa                           >  1:991140/1‑149 (MQ=255)
                                                                                                                         tAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATActc                           >  1:485415/1‑150 (MQ=255)
                                                                                                                         tAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATActc                           >  1:991130/1‑150 (MQ=255)
                                                                                                                         tAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCACCCTGGCGCAGCATAGTCAGGCATTTATActc                           >  1:133052/1‑150 (MQ=255)
                                                                                                                            cATTTGACTGGGTTGAACATAACGCCGATAGCAAAATGAGTTATTTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCttt                        <  1:1017354/150‑1 (MQ=255)
                                                                                                                             aTTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCtttt                       >  1:1294743/1‑150 (MQ=255)
                                                                                                                             aTTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCtttt                       <  2:81298/150‑1 (MQ=255)
                                                                                                                              tttGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTg                      <  2:591155/150‑1 (MQ=255)
                                                                                                                              tttGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTg                      <  2:581177/150‑1 (MQ=255)
                                                                                                                              tttGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTg                      <  2:1171388/150‑1 (MQ=255)
                                                                                                                                 gACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAAt                   >  2:698536/1‑150 (MQ=255)
                                                                                                                                 gACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAAt                   >  2:368238/1‑150 (MQ=255)
                                                                                                                                  aCTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATa                  <  2:627695/150‑1 (MQ=255)
                                                                                                                                   cTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGCTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATAc                 <  1:724179/150‑1 (MQ=255)
                                                                                                                                   aattgTTGAACATAACTCCTTTTGCTATCGGCGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATAc                 >  1:915429/5‑150 (MQ=37)
                                                                                                                                     ttgTTGAACATAACTCCTTTTGCTATCGGCGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACAt               >  1:666524/3‑150 (MQ=38)
                                                                                                                                     gggTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACAt               <  2:954756/150‑1 (MQ=255)
                                                                                                                                     gggTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACAt               >  1:1243703/1‑150 (MQ=255)
                                                                                                                                      tgTTGAACATAACTCGTTTTGCTATCGGCGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACATAGTATTCTGACTCGCCACCTGGCGCAGCAAAGTCAGGCATTTATAATCTTTTGAATACATa              >  1:75576/2‑150 (MQ=37)
                                                                                                                                      ggTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACATa              <  2:30467/150‑1 (MQ=255)
                                                                                                                                           aaCATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTAGAATAAATAGaaaa         >  1:711133/1‑150 (MQ=255)
                                                                                                                                            aCATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACCCTTTTGAATACATAGAAAAt        <  1:672963/150‑1 (MQ=255)
                                                                                                                                              aTAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACATAGAAAATTg      <  2:708737/150‑1 (MQ=255)
                                                                                                                                               tAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACATAGAAAATTGa     >  2:523186/1‑150 (MQ=255)
                                                                                                                                                 aagCCGATATCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACATAGAAAATTGata   <  1:886298/148‑1 (MQ=255)
                                                                                                                                                 aCGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACATAGAAAATTGgta   <  1:796354/150‑1 (MQ=255)
                                                                                                                                                 aCGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACATAGAAAATTGata   <  1:1024982/150‑1 (MQ=255)
                                                                                                                                                 aCGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACATAGAAAATTGatat  >  1:880639/1‑150 (MQ=255)
                                                                                                                                                  cGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACATAGAAAATTGatat  <  1:656509/150‑1 (MQ=255)
                                                                                                                                                  cGCCGATAGCAAAAGGAGTTATGTTAAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACATAGAAAATTGatat  <  1:660726/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATCCCGGCTTCATTTTTATGGCTGCACAGTAACTTTATTTGTTTAATTAACACCTAACCAGATGAAAAATTTGCTCAAAATGTTTAAATTAACTTATGTAACAGTCACGCATTATATTAAATAACATTTGACTGGGTTGAACATAACGCCGATAGCAAAAGGAGTTATGTTCAACAATTTAATTTTAGATATACATATAACTTGTTCTATTAATAAATCACCTCGTATTCTGACTCGCCCCCTGGCGCAGCAAAGTCAGGCATTTATACTCTTTTGAATACATAGAAAATTGATAT  >  NC_000913/3286969‑3287264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: