Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,289,127 A→G 5.3% Q105R (CAG→CGG)  yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,289,1270AG 5.3% 222.0 / 2.9 77Q105R (CAG→CGG) yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (35/37);  new base G (2/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

TATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTT  >  NC_000913/3288981‑3289275
                                                                                                                                                  |                                                                                                                                                    
tATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTCTCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGtt                                                                                                                                                   <  2:980599/150‑1 (MQ=255)
   gCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTAc                                                                                                                                                >  2:489294/1‑150 (MQ=255)
   gCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTAc                                                                                                                                                <  1:1201842/150‑1 (MQ=255)
   gCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGAACAACTACCAGGCACTTATCAGGTTGATATCAGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGGAGCCACAGTTTAc                                                                                                                                                <  1:68502/150‑1 (MQ=255)
    cAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACg                                                                                                                                               >  2:948383/1‑150 (MQ=255)
     aaaCAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACAGCCAATGCAGCGCTAATGCTGCAGCCACAGTTTACgt                                                                                                                                              >  1:130261/1‑149 (MQ=255)
       acaacCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTa                                                                                                                                            <  1:713004/150‑1 (MQ=255)
         aagcGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGa                                                                                                                                          <  1:374114/147‑1 (MQ=255)
             gATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTAGATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGaacaa                                                                                                                                      >  2:226473/1‑150 (MQ=255)
             gATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGaacaa                                                                                                                                      <  2:621486/150‑1 (MQ=255)
             gATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGaacaa                                                                                                                                      >  1:244887/1‑150 (MQ=255)
             gATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCAGGCTGAATAAAAAGAAGGTTTCCCAGAAAAAAATTACATTTACCGCCAAAGCAAAGCAAATACAGCAGCCACAGTTAACGCTAGaaaaa                                                                                                                                      >  1:267177/1‑150 (MQ=255)
               tCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATATACCGCCAATGCAGAGCAACATCTGCAGCCACAGTTTACGGTAGaacaacc                                                                                                                                    <  1:1081063/150‑2 (MQ=255)
                   tCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGt                                                                                                                                >  1:725184/1‑150 (MQ=255)
                    cgcgTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACATTTTACTGTAGAACAACTACGTg                                                                                                                               >  2:826321/1‑150 (MQ=255)
                     gcgGTTTTCAAAAAAATATGCACAACTACCACGAACTAATCAGGTTGAAATCTGGCTGTATAAAAAGAAGGTTTCACTGAAAAAAATTACATTTACCGCAAATGCAGAGCAACTTCTGAAGACACAGTTTACGGTATAACAACTACGtta                                                                                                                              <  2:796917/150‑3 (MQ=255)
                        tttttCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCt                                                                                                                           <  2:439827/150‑1 (MQ=255)
                           ttCAAAAAAATATGCACAAATACAAGGAACTTATCAGGTTGATATCTGGGTGAATAAAAAGAAGTTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTggg                                                                                                                        <  1:255356/150‑1 (MQ=255)
                              aaaaaaaTATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGCGCCACTTCTGCAGCCACAGTTTACGGTAGACCAACTACGTGCGCTGGGTAt                                                                                                                     >  2:1129163/1‑150 (MQ=255)
                              aaaaaaaTATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTAt                                                                                                                     <  2:1300216/150‑1 (MQ=255)
                                aaaaaTATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTa                                                                                                                   >  2:986716/1‑150 (MQ=255)
                                      atGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTgg                                                                                                             >  2:569812/1‑150 (MQ=255)
                                       tGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGa                                                                                                            <  1:290407/150‑1 (MQ=255)
                                                  cAGGAACTTATCAGGTTGATATCTGGCTGAATAAACAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCgg                                                                                                 <  2:1283934/150‑1 (MQ=255)
                                                    ggAACTTATCAGGTTGATATCAGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGgcg                                                                                               >  2:700447/1‑150 (MQ=255)
                                                        cTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACAGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGTTGGATGAAATCCCGGCgctgg                                                                                           >  1:100169/1‑150 (MQ=255)
                                                                 ttGTTATCTGGGTGTATAAATAGTATGTTTCACTGAAAAATATTACTTTTCGCGCCAATGCAGAGCAACTTCTCCAGCCACAGATTACGGTATAACAACTACGTGAGCTGGGTATTAAGGTGGTTGAAATCCCGTCGCTGGCTGAAAAAg                                                                                  <  2:237293/150‑1 (MQ=255)
                                                                 ttGATATCTGGCTGAAAAAAAATTAGGTTTCACAGAAAAAAATTACATTTAGCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTCGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAg                                                                                  <  2:198846/150‑1 (MQ=255)
                                                                  tGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGAt                                                                                >  1:509968/1‑150 (MQ=255)
                                                                   gATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGAt                                                                                <  1:986716/150‑1 (MQ=255)
                                                                     tatCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGa                                                                              <  1:559645/150‑1 (MQ=255)
                                                                      atCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGAc                                                                             <  2:353141/150‑1 (MQ=255)
                                                                       tCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTTCGTTAGCTGGGAATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACg                                                                            <  2:914697/150‑1 (MQ=255)
                                                                         tGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTCCGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGAt                                                                          <  2:364233/150‑1 (MQ=255)
                                                                          ggCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATa                                                                         >  2:1175788/1‑150 (MQ=255)
                                                                             tGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTTAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCg                                                                      >  2:1251540/1‑150 (MQ=255)
                                                                              gAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGt                                                                     >  2:400637/1‑150 (MQ=255)
                                                                                   aaaaGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCa                                                                >  1:943920/1‑150 (MQ=255)
                                                                                   aaaaGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGAAGAACAACTACGTGAGCTGGGGATTAAGGTGGTTGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCa                                                                >  1:630996/1‑150 (MQ=255)
                                                                                    aaaGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCCCAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCaa                                                               >  2:1000528/1‑150 (MQ=255)
                                                                                            tttCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTg                                                       <  1:105907/150‑1 (MQ=255)
                                                                                            tttCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTg                                                       <  2:518438/150‑1 (MQ=255)
                                                                                             ttCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGa                                                      <  2:301054/150‑1 (MQ=255)
                                                                                             ttCAAAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTTCGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGa                                                      <  2:244887/150‑1 (MQ=255)
                                                                                               cacaGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGGTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAAc                                                    <  2:847059/150‑1 (MQ=255)
                                                                                                 caGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACaa                                                  >  1:128935/1‑150 (MQ=255)
                                                                                                   taaaaaaaTTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAAt                                                <  1:1017446/149‑1 (MQ=255)
                                                                                                   gAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAAt                                                <  2:725184/150‑1 (MQ=255)
                                                                                                         aaTTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACGGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTcc                                          >  2:120915/1‑150 (MQ=255)
                                                                                                         aaTTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACGGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTcc                                          >  2:116791/1‑150 (MQ=255)
                                                                                                             aCATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGt                                      >  2:614576/1‑150 (MQ=255)
                                                                                                              cATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTa                                     <  1:437693/150‑1 (MQ=255)
                                                                                                               aTTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTAc                                    <  2:544254/150‑1 (MQ=255)
                                                                                                               aTTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTAc                                    <  1:489294/150‑1 (MQ=255)
                                                                                                                    ccGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAgctg                               <  1:321467/150‑1 (MQ=255)
                                                                                                                     cGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAgctgc                              <  2:1226636/150‑1 (MQ=255)
                                                                                                                          aTGCAGAGCATCTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAAt                         >  2:1303715/1‑150 (MQ=255)
                                                                                                                          aTGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAAt                         >  1:1111081/1‑150 (MQ=255)
                                                                                                                           tGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAAtt                        >  1:1245231/1‑150 (MQ=255)
                                                                                                                           tGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAAtt                        >  1:479266/1‑150 (MQ=255)
                                                                                                                             cAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACCAATCATTCCCGGTACAGCTGCTGAATTTg                      >  2:1236620/1‑150 (MQ=255)
                                                                                                                             cAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTg                      >  2:470736/1‑150 (MQ=255)
                                                                                                                              agagCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGa                     >  1:657102/1‑150 (MQ=255)
                                                                                                                              agagCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGATGAATTTGa                     >  1:122053/1‑150 (MQ=255)
                                                                                                                                  cAACTTCTGCAGCCACGGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTc                 <  1:116791/150‑1 (MQ=255)
                                                                                                                                  cAACTTCTGCAGCCACGGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTc                 <  1:120915/150‑1 (MQ=255)
                                                                                                                                  cAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTc                 <  2:582575/150‑1 (MQ=255)
                                                                                                                                  cAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCGGGGTATTAAGGTGGATGAAAACCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTc                 <  2:195605/150‑1 (MQ=255)
                                                                                                                                  cAACTTCTGCAGACACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTc                 <  2:629632/150‑1 (MQ=255)
                                                                                                                                    aCTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCaa               >  2:1282970/1‑150 (MQ=255)
                                                                                                                                    aCTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAat              >  1:623169/1‑150 (MQ=255)
                                                                                                                                     cTTCTGCAGCCACTGTTTACGGTAGAACAACTACGGGAGCGGGGTAGTAAGGTGGATGAAATCCCGGCGCTCGCTGAAAAAGATGACGATAGCGTGATAATCTCGCTGGAACAAATCATTCCCGGTACAGCTGCTGAATATGATTTCaaa              >  2:208811/1‑149 (MQ=255)
                                                                                                                                     cTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCATCTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAat              >  1:1198407/1‑150 (MQ=255)
                                                                                                                                     cTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAat              >  1:1308461/1‑150 (MQ=255)
                                                                                                                                         tGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTAGTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAatcatc          >  2:709401/1‑150 (MQ=255)
                                                                                                                                            aGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGc       <  2:78720/150‑1 (MQ=255)
                                                                                                                                            aGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGc       <  2:1263570/150‑1 (MQ=255)
                                                                                                                                               cacaGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAAc    >  1:888236/1‑150 (MQ=255)
                                                                                                                                                acaGTATACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACt   <  1:226473/150‑1 (MQ=255)
                                                                                                                                                 caGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACtt  <  1:745575/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TATGCAAACAACCGATCTGTCGCGTTTTTCAAAAAAATATGCACAACTACCAGGAACTTATCAGGTTGATATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAATTACATTTACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCAATCATCAGCAACTT  >  NC_000913/3288981‑3289275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: