Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,322,700 A→G 5.9% intergenic (‑195/+33) secG ← / ← glmM preprotein translocase membrane subunit/phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,322,7000AG 5.9% 252.8 / 5.7 84intergenic (‑195/+33)secG/glmMpreprotein translocase membrane subunit/phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base A (42/37);  new base G (2/3);  total (44/40)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

ATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACAC  >  NC_000913/3322553‑3322849
                                                                                                                                                   |                                                                                                                                                     
aTAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAAt                                                                                                                                                     <  2:42407/150‑1 (MQ=255)
     gCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATcgccg                                                                                                                                                >  1:835076/1‑150 (MQ=255)
     gCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATcgccg                                                                                                                                                <  2:1052830/150‑1 (MQ=255)
        ggAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGATTACTAACCAAAGCGAATGCCCTTCGCAAGGGCAATTTTATCGCATTGTAACAACTGCGGAAAAAAACAGAAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCt                                                                                                                                             >  2:56288/1‑150 (MQ=255)
          aGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATTTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCttt                                                                                                                                           >  2:564168/1‑150 (MQ=255)
             ttttAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTa                                                                                                                                        <  2:764082/150‑1 (MQ=255)
              tttAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAg                                                                                                                                       <  2:939030/150‑1 (MQ=255)
              tttAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAg                                                                                                                                       >  2:473390/1‑150 (MQ=255)
              tttAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAg                                                                                                                                       <  2:1302054/150‑1 (MQ=255)
               ttAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttttttct                                                                                                                                      <  2:984875/150‑4 (MQ=255)
                 aaTCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAACACCGAGGTGTTCGCTGAGAAAAACGGCGACAATCGCCGCCTTTTTAGCCa                                                                                                                                    >  2:236839/1‑150 (MQ=255)
                 aaTCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttttttctca                                                                                                                                    <  1:272032/150‑6 (MQ=255)
                  aTCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttttttctcag                                                                                                                                   <  2:142666/150‑7 (MQ=255)
                   tCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGt                                                                                                                                  <  1:489166/150‑1 (MQ=255)
                    cTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTATAGCCAGtt                                                                                                                                 >  2:167357/1‑150 (MQ=255)
                     tttCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTa                                                                                                                                <  1:936625/150‑1 (MQ=255)
                         ccACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTa                                                                                                                            >  2:1291771/1‑150 (MQ=255)
                         ccACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTa                                                                                                                            >  1:408891/1‑150 (MQ=255)
                          cACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTaa                                                                                                                           >  2:894563/1‑150 (MQ=255)
                          cACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTaa                                                                                                                           >  2:935617/1‑150 (MQ=255)
                             tGAAGCGGGTGTGAATACTAACCAAAGCGAATGCCCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTTAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAAGGc                                                                                                                        >  1:749660/1‑150 (MQ=255)
                               aaGCGGGTCTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATCTGTTCGCTTAGAAAAAAGTCGACAATCGCCGCCTTTTTAGACAGATATCTAACGCtt                                                                                                                      >  2:999308/1‑150 (MQ=255)
                                aGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCttt                                                                                                                     <  1:1302808/150‑1 (MQ=255)
                                aGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCttt                                                                                                                     <  1:478034/150‑1 (MQ=255)
                                aGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCttt                                                                                                                     <  1:235035/150‑1 (MQ=255)
                                    ggTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAAc                                                                                                                 <  2:310251/150‑1 (MQ=255)
                                     gtgtGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGACAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTAATCTAACGCTTTAAACg                                                                                                                <  2:174407/150‑1 (MQ=255)
                                           aTACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCtttt                                                                                                          <  1:1186561/150‑1 (MQ=255)
                                           aTACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCtttt                                                                                                          <  2:19900/150‑1 (MQ=255)
                                              cTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACt                                                                                                       <  2:338068/150‑1 (MQ=255)
                                                aaCCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAACAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGc                                                                                                     >  1:34273/1‑150 (MQ=255)
                                                aaCCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGc                                                                                                     >  2:1109444/1‑150 (MQ=255)
                                                 aCCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCa                                                                                                    >  1:648300/1‑150 (MQ=255)
                                                 aCCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCa                                                                                                    >  1:208433/1‑150 (MQ=255)
                                                     aaGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCgg                                                                                                >  1:891823/1‑150 (MQ=255)
                                                      aGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACATCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGc                                                                                               <  1:974460/150‑1 (MQ=255)
                                                           aTGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGc                                                                                          >  1:380239/1‑150 (MQ=255)
                                                           aTGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGc                                                                                          >  1:385589/1‑150 (MQ=255)
                                                             gACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCgg                                                                                        >  2:1216520/1‑150 (MQ=255)
                                                               ccTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCgggg                                                                                      >  1:248814/1‑148 (MQ=255)
                                                               ccTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgtg                                                                                      <  1:326217/150‑1 (MQ=255)
                                                               ccTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgtg                                                                                      >  1:439355/1‑150 (MQ=255)
                                                                cTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgtgt                                                                                     >  1:903662/1‑150 (MQ=255)
                                                                cTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACCGCATCGCCGATGCAgtaa                                                                                     <  2:442664/150‑3 (MQ=255)
                                                                 ttCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGtgtg                                                                                    <  1:173670/150‑1 (MQ=255)
                                                                     cAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCAGAGAAAAAAGGCGACAAACGCCGCCTTTTTAGCCAGTTATCTAACGCTATAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCaaa                                                                                >  1:1297462/1‑150 (MQ=255)
                                                                        gggCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGCCAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTc                                                                             >  2:9784/1‑150 (MQ=255)
                                                                        gggCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTc                                                                             <  1:1179062/150‑1 (MQ=255)
                                                                          gCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAg                                                                           <  1:473390/150‑1 (MQ=255)
                                                                           cAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGCCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGt                                                                          >  2:327177/1‑150 (MQ=255)
                                                                            aaTTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTc                                                                         <  1:263816/150‑1 (MQ=255)
                                                                                     gCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCTGTGTGCAAATTCAGTAACCTGCGCt                                                                >  1:231475/1‑150 (MQ=255)
                                                                                        ttGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCGAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCg                                                             <  1:356597/150‑1 (MQ=255)
                                                                                           tATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCt                                                          <  2:385589/150‑1 (MQ=255)
                                                                                           tATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCt                                                          <  2:380239/150‑1 (MQ=255)
                                                                                           tATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGAGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCt                                                          >  1:632220/1‑150 (MQ=255)
                                                                                              cAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGGGTGCAAATTCAGTCACCTGCGCTTCGTCTTCg                                                       >  2:774565/1‑150 (MQ=255)
                                                                                               aaCTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGc                                                      <  2:213278/150‑1 (MQ=255)
                                                                                                aCTGCGGAAAAAAACCGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTAACTGCATCGGCGATGAGGTCTGCCAATTCAGTCACATGCGCTTCGTCTTCGcc                                                     >  1:813519/1‑150 (MQ=255)
                                                                                                  tGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTACGCCtt                                                   >  1:515840/1‑150 (MQ=255)
                                                                                                  tGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTCAGAAAAAAGCCGACAATCGCCGCCTTATTAGCCAGATATCTAAGCCTTTAAACGGCTTTTACTGCATCGGCGATGCAGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCta                                                   >  2:1093785/1‑149 (MQ=255)
                                                                                                   gCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTc                                                  >  2:445786/1‑150 (MQ=255)
                                                                                                     ggAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTcca                                                <  1:1015736/150‑1 (MQ=255)
                                                                                                      gAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTccac                                               >  2:325253/1‑150 (MQ=255)
                                                                                                       aaaaaaaCAGCAAAACCGCTGTGGTCGCGGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTccacc                                              <  2:34273/150‑1 (MQ=255)
                                                                                                           aaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCa                                          <  1:987447/150‑1 (MQ=255)
                                                                                                               aGCAAAACCGATGTGATCGCTGAGAAAAAAGTCGACACTCGCCGCCTTTTTAGCCAGGAATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCAcgcg                                      <  1:864786/150‑1 (MQ=255)
                                                                                                                 cAAAACCTATGTGTTCTCTTATAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAAt                                   <  1:15824/150‑1 (MQ=255)
                                                                                                                  aaaaCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGGCTTCGCCTTCCACCATCACGCGAAt                                   <  1:798570/150‑1 (MQ=255)
                                                                                                                    aaCCGATGAGCTCGCTGAGAAAAACGGCGACAATCGCCGCCGTTTTAGCCAGTTATCAAAAGCTTTAAACGGCTTTTACAGCATCGTCGATGCGGTGTGCAAATTCAGTCCCCTGCGCTTCCTCTTCGCCTTCCACCATCACGCGAATTa                                 <  1:212473/150‑1 (MQ=255)
                                                                                                                        gATTTGTTCGCTGAGAATAAAGTCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAActg                             <  2:1231421/150‑3 (MQ=255)
                                                                                                                           gtgtTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGTCTTTTACTGCTTCGGCGATGCGGTGTGCAAATTCCGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCAGGCGAATTCACGGGTc                          >  2:918619/1‑150 (MQ=255)
                                                                                                                            tgtTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCg                         <  1:564168/150‑1 (MQ=255)
                                                                                                                                cGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCCCGCGAATTAACGGTTCGGTGc                     >  2:374265/1‑150 (MQ=255)
                                                                                                                                cGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTTACGGTTCGGTGc                     >  1:968925/1‑150 (MQ=255)
                                                                                                                                cGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGc                     >  2:1288086/1‑150 (MQ=255)
                                                                                                                                  cTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCg                   >  1:1013044/1‑150 (MQ=255)
                                                                                                                                   tGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCgg                  <  2:411454/150‑1 (MQ=255)
                                                                                                                                       taaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGAttt              >  1:371192/2‑150 (MQ=255)
                                                                                                                                       gaaaaaGGCGACAATCGCCGCCTTTTTAGCCAGGTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTCTGCAAATTCAGTCACCAGCCCTACGTCTTCGCGTTCCACCATCACGCGAATGAACGGTTCGGTGCCGGAttt              >  2:697819/2‑150 (MQ=255)
                                                                                                                                         aaacGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAACCGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGGCTACGCCATCCACCCACACGCGATCTAACGGTTCGGTGCCGGATTTAc            >  2:808287/1‑150 (MQ=255)
                                                                                                                                         aaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTAc            >  2:219813/1‑150 (MQ=255)
                                                                                                                                             ggCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACgcag        >  2:1065454/1‑150 (MQ=255)
                                                                                                                                                  cAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAaca   <  2:1015517/150‑1 (MQ=255)
                                                                                                                                                   aaTCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAacac  <  2:905412/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATAAAGCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACAC  >  NC_000913/3322553‑3322849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: