Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,437,908 T→A 5.3% intergenic (+14/‑116) trkA → / → mscL NAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,437,9080TA 5.3% 258.0 / 3.2 92intergenic (+14/‑116)trkA/mscLNAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance
Reads supporting (aligned to +/- strand):  ref base T (45/41);  new base A (3/2);  total (49/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

ACGTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCG  >  NC_000913/3437759‑3438056
                                                                                                                                                     |                                                                                                                                                    
aCGTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCt                                                                                                                                                      <  2:916977/150‑1 (MQ=255)
  gTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTaa                                                                                                                                                    >  2:1104463/1‑150 (MQ=255)
    ggAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATg                                                                                                                                                  <  1:1049411/150‑1 (MQ=255)
     gAAAAGAAGTGATGAATGCCAATGAAAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACATATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGa                                                                                                                                                 <  1:1184813/150‑1 (MQ=255)
      aaaCGACGTGATGATTGCCAATGACAATATGCTCATTGAGCAAGGCGATCAAGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGAc                                                                                                                                                <  1:213970/150‑1 (MQ=255)
         cgacgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGcc                                                                                                                                             >  1:374181/1‑150 (MQ=255)
            cgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCtta                                                                                                                                          <  1:71378/150‑1 (MQ=255)
            cgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCtta                                                                                                                                          <  1:77786/150‑1 (MQ=255)
              tgatgaTTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                        <  1:1126580/150‑1 (MQ=255)
               gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatta                                                                                                                                       <  2:711208/150‑1 (MQ=255)
                 tgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTattatt                                                                                                                                     >  1:286327/1‑150 (MQ=255)
                  tatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                    <  2:1137201/149‑1 (MQ=255)
                    ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTcc                                                                                                                                  <  2:907257/150‑1 (MQ=255)
                     tGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTccc                                                                                                                                 <  2:343608/150‑1 (MQ=255)
                      gCCAATGACAATCTGCCCATTGAGCAAGGCGATCACTTTATTATGTTCCTCACAGCTAAAAAGTTTATTACCGACGGCGAAAGTCTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCt                                                                                                                                <  2:94686/150‑1 (MQ=255)
                         aaTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAAAGACGCCTTATTATTTCCCTTTg                                                                                                                             <  1:246313/150‑1 (MQ=255)
                             aCAATCTGCGCATTGAGCAAGGCGAAAACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTACCGCCAAGCCATTTCTTCTTGTAATTAATAAGGCTTCTAATGACGCCTTATTTTTTCCCTTTGATTa                                                                                                                         >  1:618347/1‑150 (MQ=255)
                                aTCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCa                                                                                                                      <  1:172373/150‑1 (MQ=255)
                                      gcATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGTCGCCTTATTATTTCCCTTTGATTATCAAGGatt                                                                                                                <  1:695523/150‑1 (MQ=255)
                                      gaaaagAGCAAGGCGATCACTTAATTTGGTTCCTCAAAGATTAAAAGTTTATTACCGACGTCGAAAGAATCTTCCAGCCAAGCCCTTTCAGCTAGTAAATAATAAGGCGTCTAATGACGCCTTATTAATTCCCTTTGATTATCAAGGatt                                                                                                                <  2:184497/145‑1 (MQ=255)
                                       cATTGAGCAAGTCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCATTTCTTCTTGTAATTAATAACGCTACTAATGACTCCTTATTATTTCACTTTAATTATCAAgtaata                                                                                                               >  2:287912/1‑145 (MQ=255)
                                         ttGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaat                                                                                                             <  1:349718/150‑1 (MQ=255)
                                         ttGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaat                                                                                                             <  1:1212072/150‑1 (MQ=255)
                                          tGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaatta                                                                                                           <  2:293241/150‑1 (MQ=255)
                                          tGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaatta                                                                                                           <  2:21469/150‑1 (MQ=255)
                                           gAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCACGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaatta                                                                                                           >  2:711406/1‑150 (MQ=255)
                                             gCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGTCGTCTAATGACGCCTTATTATTTCCCTGTGATTATCAAGGattatttatt                                                                                                         >  2:1307571/1‑148 (MQ=255)
                                                 ggCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAattca                                                                                                     <  1:578037/150‑1 (MQ=255)
                                                  gCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATAACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATTAAGCATTATTATTTAAATATGATTATAAAGGATTAATTAAattcat                                                                                                    >  2:570160/1‑150 (MQ=255)
                                                   cGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACTCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAattcatt                                                                                                   <  1:636417/150‑1 (MQ=255)
                                                     aTCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCCGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTcc                                                                                                 >  2:1138749/1‑150 (MQ=255)
                                                       cACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTg                                                                                               <  2:585215/150‑1 (MQ=255)
                                                        aCGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTgg                                                                                              >  2:488291/1‑150 (MQ=255)
                                                           tAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAg                                                                                           <  2:1256393/150‑1 (MQ=255)
                                                               tATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGTCAGGaaa                                                                                       >  1:1174695/1‑150 (MQ=255)
                                                               tATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGaaa                                                                                       >  1:1180106/1‑150 (MQ=255)
                                                               tATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGaaa                                                                                       >  2:944406/1‑150 (MQ=255)
                                                               tATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCATTCTTATTGTAATTAATAAGGCTTCTAATGACGCATTATTATTTCCCTTTGATTAACAAGGATTAATTAAATACATTCCTGTCAGCaaa                                                                                       >  1:283713/1‑150 (MQ=255)
                                                                  tttCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAATCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATg                                                                                    <  1:339614/149‑1 (MQ=255)
                                                                   ttCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGTATTAATTAAATTCATTCCTGGCAGGAAAATgg                                                                                   >  1:1016607/1‑150 (MQ=255)
                                                                    tCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTGTTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGc                                                                                  <  2:1075276/150‑1 (MQ=255)
                                                                          caGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACa                                                                            >  1:489489/1‑150 (MQ=255)
                                                                          aaGATAAAAAGTTTATAACCGAGGTCGAAAGACTTTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCGTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAATATGGCTTAACa                                                                            <  2:43821/149‑1 (MQ=255)
                                                                              tAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTg                                                                        >  2:333962/1‑150 (MQ=255)
                                                                               aaaaaGTTTATTACCGACGTCTAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGt                                                                       <  1:887874/150‑1 (MQ=255)
                                                                               aaaaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGt                                                                       >  2:750807/1‑150 (MQ=255)
                                                                                aaaaGTTTATTACCGTCGTCGAAAGACTCTTCCAGCCATGCCCTTTATTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGtt                                                                      <  2:20113/150‑1 (MQ=255)
                                                                                 aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                     <  1:1267902/150‑1 (MQ=255)
                                                                                 aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                     <  1:305133/150‑1 (MQ=255)
                                                                                     tttATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACt                                                                 <  2:284394/150‑1 (MQ=255)
                                                                                       tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTa                                                               >  1:1083881/1‑150 (MQ=255)
                                                                                          taCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGAAGCCATATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATATGTTAGACTTATgt                                                            >  1:482025/1‑149 (MQ=255)
                                                                                           aCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTGATTATTTCCCTTTGATTATCAAGGATTAATTATATTAATTCCTGGCAGAAAAATGGCTTAACATTTGTTAGACTTATGGt                                                           >  1:189790/1‑150 (MQ=255)
                                                                                                 gTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCgg                                                     <  2:733780/150‑1 (MQ=255)
                                                                                                    gAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCtt                                                  <  2:405890/150‑1 (MQ=255)
                                                                                                    gAAAGACTCTTCCAGCCAAGCCCTTTCCTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCtt                                                  >  2:271070/1‑150 (MQ=255)
                                                                                                     aaaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTc                                                 >  1:288227/1‑150 (MQ=255)
                                                                                                      aaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCa                                                <  1:467729/150‑1 (MQ=255)
                                                                                                      aaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCa                                                <  2:97276/150‑1 (MQ=255)
                                                                                                          ctctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTAGTCTTAATatt                                            >  1:844178/1‑148 (MQ=255)
                                                                                                            tttCGAGCCAAGCCCTTTCTTGTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGAGTTATGGTTGTCGGCTTCATAGGga                                          <  2:423964/149‑1 (MQ=255)
                                                                                                             ttCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGgag                                         >  2:154892/1‑150 (MQ=255)
                                                                                                              tCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGCCGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGgaga                                        >  2:159495/1‑150 (MQ=255)
                                                                                                                  gCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATaa                                    >  1:495954/1‑150 (MQ=255)
                                                                                                                   ccAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATaaa                                   >  2:1119255/1‑149 (MQ=255)
                                                                                                                    cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGCCGCCTTATTATTTCGCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACa                                  >  1:1127344/1‑150 (MQ=255)
                                                                                                                    cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGATTAACa                                  >  1:1220648/1‑150 (MQ=255)
                                                                                                                    cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACa                                  <  1:90511/150‑1 (MQ=255)
                                                                                                                    cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATAATCAATGATTTATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTATACTTATGGTTGTCGGCTTCATAGGGAGAATAACa                                  <  2:72863/150‑1 (MQ=255)
                                                                                                                     aaGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACAt                                 >  2:891872/1‑150 (MQ=255)
                                                                                                                      aGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATg                                <  2:1291681/150‑1 (MQ=255)
                                                                                                                      aGCCCTTTCTTCTTGTAATTAATAAGGCGGCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATg                                <  2:618347/150‑1 (MQ=255)
                                                                                                                        cccTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTGTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAg                              >  1:844863/1‑150 (MQ=255)
                                                                                                                          cTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCa                            >  1:615682/1‑150 (MQ=255)
                                                                                                                              cttcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCattat                        >  2:210920/1‑150 (MQ=255)
                                                                                                                               ttcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCattatt                       >  2:1082092/1‑150 (MQ=255)
                                                                                                                                tcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCAttatta                      <  1:112719/150‑1 (MQ=255)
                                                                                                                                   tGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAg                   >  1:1084010/1‑150 (MQ=255)
                                                                                                                                     taattaatAAGGCGTCGAATGACGCCTTATTATTTCCCTTTGATTATCAATGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGaa                 >  1:90745/1‑150 (MQ=255)
                                                                                                                                      aattaatAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAt                >  2:689402/1‑150 (MQ=255)
                                                                                                                                          aataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcg            <  1:488291/150‑1 (MQ=255)
                                                                                                                                           ataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTATATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGGTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc           >  2:610193/1‑150 (MQ=255)
                                                                                                                                           ataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc           >  2:137128/1‑150 (MQ=255)
                                                                                                                                           ataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTagc           >  1:37782/1‑150 (MQ=255)
                                                                                                                                            taacgagTCTAAAGACGCCTAATTATTTCCCTTTGATTTTCAATGATTAATTAAATTCAATCCTGGCACGAAAATGGCTTAACATTTGTAAGACTTATGGCTGTCGGCTTCATATGGAGAATAACATGAGCATTATTAAAGAATTTcgcg          <  1:115595/144‑1 (MQ=255)
                                                                                                                                            taaGGCGTCTAATGCCGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATACCATGAGCATTATTAAAGAATTTcgcg          >  2:938398/1‑150 (MQ=255)
                                                                                                                                             aaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCTCGa         >  1:941840/1‑150 (MQ=255)
                                                                                                                                             aaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGCGAGAATAACATGAGCATTATTAAAGAATTTCGCGa         >  1:69217/1‑150 (MQ=255)
                                                                                                                                             aaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATAAAATTCATTCCTGGAAGGAAAATCGCTTAACATTTCTTAGACTAATGGTTGTCGTCTTCATAGGGAGAATAAAATGATCATTATTAAAGAATATCTCGa         >  1:582458/1‑150 (MQ=255)
                                                                                                                                               ggCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAAt       >  2:894608/1‑150 (MQ=255)
                                                                                                                                                 cGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAAttt     >  2:304412/1‑150 (MQ=255)
                                                                                                                                                  gTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg    >  2:1164180/1‑150 (MQ=255)
                                                                                                                                                    cTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTAGCGAATTTGcc  >  2:489329/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACGTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCG  >  NC_000913/3437759‑3438056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: