Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,437,909 A→T 5.5% intergenic (+15/‑115) trkA → / → mscL NAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,437,9090AT 5.5% 269.9 / 3.9 91intergenic (+15/‑115)trkA/mscLNAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance
Reads supporting (aligned to +/- strand):  ref base A (45/41);  new base T (3/2);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

GTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGAT  >  NC_000913/3437761‑3438058
                                                                                                                                                    |                                                                                                                                                     
gTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTaa                                                                                                                                                      >  2:1104463/1‑150 (MQ=255)
  ggAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATg                                                                                                                                                    <  1:1049411/150‑1 (MQ=255)
   gAAAAGAAGTGATGAATGCCAATGAAAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACATATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGa                                                                                                                                                   <  1:1184813/150‑1 (MQ=255)
    aaaCGACGTGATGATTGCCAATGACAATATGCTCATTGAGCAAGGCGATCAAGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGAc                                                                                                                                                  <  1:213970/150‑1 (MQ=255)
       cgacgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGcc                                                                                                                                               >  1:374181/1‑150 (MQ=255)
          cgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCtta                                                                                                                                            <  1:77786/150‑1 (MQ=255)
          cgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCtta                                                                                                                                            <  1:71378/150‑1 (MQ=255)
            tgatgaTTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                          <  1:1126580/150‑1 (MQ=255)
             gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatta                                                                                                                                         <  2:711208/150‑1 (MQ=255)
               tgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTattatt                                                                                                                                       >  1:286327/1‑150 (MQ=255)
                tatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTAttt                                                                                                                                      <  2:1137201/149‑1 (MQ=255)
                  ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTcc                                                                                                                                    <  2:907257/150‑1 (MQ=255)
                   tGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTccc                                                                                                                                   <  2:343608/150‑1 (MQ=255)
                    gCCAATGACAATCTGCCCATTGAGCAAGGCGATCACTTTATTATGTTCCTCACAGCTAAAAAGTTTATTACCGACGGCGAAAGTCTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCt                                                                                                                                  <  2:94686/150‑1 (MQ=255)
                       aaTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAAAGACGCCTTATTATTTCCCTTTg                                                                                                                               <  1:246313/150‑1 (MQ=255)
                           aCAATCTGCGCATTGAGCAAGGCGAAAACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTACCGCCAAGCCATTTCTTCTTGTAATTAATAAGGCTTCTAATGACGCCTTATTTTTTCCCTTTGATTa                                                                                                                           >  1:618347/1‑150 (MQ=255)
                              aTCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCa                                                                                                                        <  1:172373/150‑1 (MQ=255)
                                    gcATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGTCGCCTTATTATTTCCCTTTGATTATCAAGGatt                                                                                                                  <  1:695523/150‑1 (MQ=255)
                                    gaaaagAGCAAGGCGATCACTTAATTTGGTTCCTCAAAGATTAAAAGTTTATTACCGACGTCGAAAGAATCTTCCAGCCAAGCCCTTTCAGCTAGTAAATAATAAGGCGTCTAATGACGCCTTATTAATTCCCTTTGATTATCAAGGatt                                                                                                                  <  2:184497/145‑1 (MQ=255)
                                     cATTGAGCAAGTCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCATTTCTTCTTGTAATTAATAACGCTACTAATGACTCCTTATTATTTCACTTTAATTATCAAgtaata                                                                                                                 >  2:287912/1‑145 (MQ=255)
                                       ttGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaat                                                                                                               <  1:349718/150‑1 (MQ=255)
                                       ttGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaat                                                                                                               <  1:1212072/150‑1 (MQ=255)
                                        tGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaatta                                                                                                             <  2:293241/150‑1 (MQ=255)
                                        tGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaatta                                                                                                             <  2:21469/150‑1 (MQ=255)
                                         gAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCACGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaatta                                                                                                             >  2:711406/1‑150 (MQ=255)
                                           gCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGTCGTCTAATGACGCCTTATTATTTCCCTGTGATTATCAAGGattatttatt                                                                                                           >  2:1307571/1‑148 (MQ=255)
                                               ggCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAattca                                                                                                       <  1:578037/150‑1 (MQ=255)
                                                gCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATAACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATTAAGCATTATTATTTAAATATGATTATAAAGGATTAATTAAattcat                                                                                                      >  2:570160/1‑150 (MQ=255)
                                                 cGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACTCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAattcatt                                                                                                     <  1:636417/150‑1 (MQ=255)
                                                   aTCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCCGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTcc                                                                                                   >  2:1138749/1‑150 (MQ=255)
                                                     cACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTg                                                                                                 <  2:585215/150‑1 (MQ=255)
                                                      aCGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTgg                                                                                                >  2:488291/1‑150 (MQ=255)
                                                         tAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAg                                                                                             <  2:1256393/150‑1 (MQ=255)
                                                             tATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGTCAGGaaa                                                                                         >  1:1174695/1‑150 (MQ=255)
                                                             tATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGaaa                                                                                         >  2:944406/1‑150 (MQ=255)
                                                             tATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGaaa                                                                                         >  1:1180106/1‑150 (MQ=255)
                                                             tATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCATTCTTATTGTAATTAATAAGGCTTCTAATGACGCATTATTATTTCCCTTTGATTAACAAGGATTAATTAAATACATTCCTGTCAGCaaa                                                                                         >  1:283713/1‑150 (MQ=255)
                                                                tttCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAATCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATg                                                                                      <  1:339614/149‑1 (MQ=255)
                                                                 ttCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGTATTAATTAAATTCATTCCTGGCAGGAAAATgg                                                                                     >  1:1016607/1‑150 (MQ=255)
                                                                  tCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTGTTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGc                                                                                    <  2:1075276/150‑1 (MQ=255)
                                                                        caGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACa                                                                              >  1:489489/1‑150 (MQ=255)
                                                                        aaGATAAAAAGTTTATAACCGAGGTCGAAAGACTTTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCGTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAATATGGCTTAACa                                                                              <  2:43821/149‑1 (MQ=255)
                                                                            tAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTg                                                                          >  2:333962/1‑150 (MQ=255)
                                                                             aaaaaGTTTATTACCGACGTCTAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGt                                                                         <  1:887874/150‑1 (MQ=255)
                                                                             aaaaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGt                                                                         >  2:750807/1‑150 (MQ=255)
                                                                              aaaaGTTTATTACCGTCGTCGAAAGACTCTTCCAGCCATGCCCTTTATTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGtt                                                                        <  2:20113/150‑1 (MQ=255)
                                                                               aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                       <  1:1267902/150‑1 (MQ=255)
                                                                               aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                       <  1:305133/150‑1 (MQ=255)
                                                                                   tttATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACt                                                                   <  2:284394/150‑1 (MQ=255)
                                                                                     tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTa                                                                 >  1:1083881/1‑150 (MQ=255)
                                                                                        taCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGAAGCCATATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATATGTTAGACTTATgt                                                              >  1:482025/1‑149 (MQ=255)
                                                                                         aCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTGATTATTTCCCTTTGATTATCAAGGATTAATTATATTAATTCCTGGCAGAAAAATGGCTTAACATTTGTTAGACTTATGGt                                                             >  1:189790/1‑150 (MQ=255)
                                                                                               gTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCgg                                                       <  2:733780/150‑1 (MQ=255)
                                                                                                  gAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCtt                                                    <  2:405890/150‑1 (MQ=255)
                                                                                                  gAAAGACTCTTCCAGCCAAGCCCTTTCCTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCtt                                                    >  2:271070/1‑150 (MQ=255)
                                                                                                   aaaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTc                                                   >  1:288227/1‑150 (MQ=255)
                                                                                                    aaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCa                                                  <  1:467729/150‑1 (MQ=255)
                                                                                                    aaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCa                                                  <  2:97276/150‑1 (MQ=255)
                                                                                                        ctctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTAGTCTTAATatt                                              >  1:844178/1‑148 (MQ=255)
                                                                                                          tttCGAGCCAAGCCCTTTCTTGTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGAGTTATGGTTGTCGGCTTCATAGGga                                            <  2:423964/149‑1 (MQ=255)
                                                                                                           ttCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGgag                                           >  2:154892/1‑150 (MQ=255)
                                                                                                            tCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGCCGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGgaga                                          >  2:159495/1‑150 (MQ=255)
                                                                                                                gCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATaa                                      >  1:495954/1‑150 (MQ=255)
                                                                                                                 ccAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATaaa                                     >  2:1119255/1‑149 (MQ=255)
                                                                                                                  cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGCCGCCTTATTATTTCGCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACa                                    >  1:1127344/1‑150 (MQ=255)
                                                                                                                  cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGATTAACa                                    >  1:1220648/1‑150 (MQ=255)
                                                                                                                  cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACa                                    <  1:90511/150‑1 (MQ=255)
                                                                                                                  cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATAATCAATGATTTATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTATACTTATGGTTGTCGGCTTCATAGGGAGAATAACa                                    <  2:72863/150‑1 (MQ=255)
                                                                                                                   aaGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACAt                                   >  2:891872/1‑150 (MQ=255)
                                                                                                                    aGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATg                                  <  2:1291681/150‑1 (MQ=255)
                                                                                                                    aGCCCTTTCTTCTTGTAATTAATAAGGCGGCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATg                                  <  2:618347/150‑1 (MQ=255)
                                                                                                                      cccTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTGTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAg                                >  1:844863/1‑150 (MQ=255)
                                                                                                                        cTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCa                              >  1:615682/1‑150 (MQ=255)
                                                                                                                            cttcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCattat                          >  2:210920/1‑150 (MQ=255)
                                                                                                                             ttcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCattatt                         >  2:1082092/1‑150 (MQ=255)
                                                                                                                              tcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCAttatta                        <  1:112719/150‑1 (MQ=255)
                                                                                                                                 tGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAg                     >  1:1084010/1‑150 (MQ=255)
                                                                                                                                   taattaatAAGGCGTCGAATGACGCCTTATTATTTCCCTTTGATTATCAATGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGaa                   >  1:90745/1‑150 (MQ=255)
                                                                                                                                    aattaatAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAt                  >  2:689402/1‑150 (MQ=255)
                                                                                                                                        aataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcg              <  1:488291/150‑1 (MQ=255)
                                                                                                                                         ataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTATATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGGTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc             >  2:610193/1‑150 (MQ=255)
                                                                                                                                         ataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc             >  2:137128/1‑150 (MQ=255)
                                                                                                                                         ataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTagc             >  1:37782/1‑150 (MQ=255)
                                                                                                                                          taacgagTCTAAAGACGCCTAATTATTTCCCTTTGATTTTCAATGATTAATTAAATTCAATCCTGGCACGAAAATGGCTTAACATTTGTAAGACTTATGGCTGTCGGCTTCATATGGAGAATAACATGAGCATTATTAAAGAATTTcgcg            <  1:115595/144‑1 (MQ=255)
                                                                                                                                          taaGGCGTCTAATGCCGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATACCATGAGCATTATTAAAGAATTTcgcg            >  2:938398/1‑150 (MQ=255)
                                                                                                                                           aaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCTCGa           >  1:941840/1‑150 (MQ=255)
                                                                                                                                           aaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGCGAGAATAACATGAGCATTATTAAAGAATTTCGCGa           >  1:69217/1‑150 (MQ=255)
                                                                                                                                           aaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATAAAATTCATTCCTGGAAGGAAAATCGCTTAACATTTCTTAGACTAATGGTTGTCGTCTTCATAGGGAGAATAAAATGATCATTATTAAAGAATATCTCGa           >  1:582458/1‑150 (MQ=255)
                                                                                                                                             ggCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAAt         >  2:894608/1‑150 (MQ=255)
                                                                                                                                               cGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAAttt       >  2:304412/1‑150 (MQ=255)
                                                                                                                                                gTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg      >  2:1164180/1‑150 (MQ=255)
                                                                                                                                                  cTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTAGCGAATTTGcc    >  2:489329/1‑149 (MQ=255)
                                                                                                                                                    aaTGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGATTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATACCATGAGCATAATTAAAGAATTTCGCGAATTTGCGAt  >  2:633892/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGAT  >  NC_000913/3437761‑3438058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: