Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,448,276 T→A 7.8% intergenic (‑127/+38) rplN ← / ← rpsQ 50S ribosomal subunit protein L14/30S ribosomal subunit protein S17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,448,2760TA 7.8% 231.4 / 7.4 87intergenic (‑127/+38)rplN/rpsQ50S ribosomal subunit protein L14/30S ribosomal subunit protein S17
Reads supporting (aligned to +/- strand):  ref base T (36/44);  new base A (5/2);  total (41/46)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

CATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTTCTC  >  NC_000913/3448129‑3448424
                                                                                                                                                   |                                                                                                                                                    
cATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAttta                                                                                                                                                    >  2:1263655/1‑149 (MQ=255)
    gTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACCCCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAg                                                                                                                                                <  2:314702/150‑1 (MQ=255)
     tCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGTTAGAAAAAATAAACGGCTCATTTCTGAGc                                                                                                                                               <  1:1176328/150‑1 (MQ=255)
     tCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGc                                                                                                                                               <  1:625683/150‑1 (MQ=255)
      ttGTTCTTGGTTCATTTTAGTGCTGCGCTATTGGCAACTACTACTGAGACCCGAATATCAGGTGGTTAAAAATCTCCATTTCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGcc                                                                                                                                              <  2:679572/149‑1 (MQ=255)
        gTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGt                                                                                                                                            <  1:298168/150‑1 (MQ=255)
         ttCTTGGACCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGtt                                                                                                                                           <  1:923825/150‑1 (MQ=255)
            ttGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATTACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCCTAGCCGTTTAt                                                                                                                                        >  1:657402/1‑150 (MQ=255)
             tGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTAtt                                                                                                                                       >  1:735857/1‑150 (MQ=255)
             tGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTAtt                                                                                                                                       >  1:523725/1‑150 (MQ=255)
              ggATCATTTTAGTGCTCAGATAATGTCAAATCCTACTCAGACCCGAAACAATGGTCGTGAAAAATCCCCATATCGTGGGCGCGTAATTATATCACGGCTTCATTGATATGGGTTGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTc                                                                                                                                      <  2:974254/150‑1 (MQ=255)
               taTCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACACGATAATCAGGTCGTTAAAACTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCg                                                                                                                                     <  1:812941/149‑1 (MQ=255)
               gATCATTTTAGTGCTCCTCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCg                                                                                                                                     <  1:774070/150‑1 (MQ=255)
                 tCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAGAAATGAGCCGtt                                                                                                                                           <  2:413462/142‑1 (MQ=255)
                 tCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAGAAATGAGCCGtt                                                                                                                                           >  1:413462/1‑142 (MQ=255)
                      ttAGTGCTCCGCTAATGTCAACTACTACTGAGACCCTAAAATCAGGTCGTTAAAAATCCCCATATCGCGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTgag                                                                                                                              <  1:1077593/150‑1 (MQ=255)
                      ttAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACTCCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTgag                                                                                                                              <  2:145914/150‑1 (MQ=255)
                      ttAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTgag                                                                                                                              >  2:1265395/1‑150 (MQ=255)
                        aGTGCTCCGCTAATGTCAACTCCTACTGAGACCCTAAAATCAGGGCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATACACGGCTCATTTCTGAGCCGTTTATTCGTATTgagag                                                                                                                            <  1:646908/150‑1 (MQ=255)
                        aGTGCTCCGCTAATGTCAACTACTACTGATACCCTAAAATCAGCTCGTTAAAAATCCCCATATCGAGTGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTgagag                                                                                                                            <  1:642404/150‑1 (MQ=255)
                          tGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGagagag                                                                                                                          <  1:750578/150‑1 (MQ=255)
                          tGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGagagag                                                                                                                          <  1:1214748/150‑1 (MQ=255)
                           gCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGTGAGAgt                                                                                                                         >  2:1312823/1‑150 (MQ=255)
                            ctcctcTAATGTCAACTACTACTGAGACCCTAAAATCAGGGCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAATGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAgtg                                                                                                                        <  1:978308/150‑1 (MQ=255)
                            caccGCTAATTTCAACTACTACTGACACCCGAAAATCAGGTCGTAAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAgtg                                                                                                                        <  2:601197/148‑1 (MQ=255)
                             tgcGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTGCTGAGCCGTTTATTCGTATTGAGAGAgtgt                                                                                                                       <  1:900072/148‑1 (MQ=255)
                             tCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAgtgt                                                                                                                       <  1:61162/150‑1 (MQ=255)
                                 cTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTg                                                                                                                   <  2:735447/150‑1 (MQ=255)
                                   aaTGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCATCAAGGATATGGGTAGAAAAAATAACCGGCTAATTTCTGAGCCGTTTATACGTATTGAGAGAGTGTaatatt                                                                                                                 >  1:255543/1‑145 (MQ=255)
                                        cAACTACTACTGAGACCCGAACATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAATGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACa                                                                                                            <  2:912415/150‑1 (MQ=255)
                                        cAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGAGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACa                                                                                                            <  2:713196/150‑1 (MQ=255)
                                          actactactGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGa                                                                                                          <  2:342547/150‑1 (MQ=255)
                                             actactGAGCCCCGATAATCAGGTCGTTAAAAATCACCATATCTAGGGCGAGGCATTATAACCCCTCGTCAAGGATATGGGTAGAATAATTAAACGGCTCATTTCTGAGCCGTTTATTCGTATTTAGAGAGTGTACTGTATTACAGAAcc                                                                                                       <  2:206247/150‑1 (MQ=255)
                                                 ctGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTTTACTGTATTACAGAACCGCtt                                                                                                   >  1:1239446/1‑150 (MQ=255)
                                                   tagaCCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTtc                                                                                                 <  2:62718/149‑1 (MQ=255)
                                                      aCCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTctct                                                                                              <  1:309925/150‑1 (MQ=255)
                                                       cccGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTa                                                                                             >  1:1303505/1‑150 (MQ=255)
                                                       cccGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTAGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTa                                                                                             >  1:663218/1‑150 (MQ=255)
                                                         cGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACa                                                                                           >  2:6241/1‑150 (MQ=255)
                                                             aaTCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAAcgc                                                                                       <  1:1054390/150‑1 (MQ=255)
                                                              aTCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATAACAGACCCGCTTTCTCTACAAcgcg                                                                                      >  1:60391/1‑150 (MQ=255)
                                                                  ggTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAATTCTGAGCCGTATATTAGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAAcc                                                                                  >  2:628493/1‑150 (MQ=255)
                                                                         aaaaaTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTcc                                                                           >  1:681957/1‑150 (MQ=255)
                                                                             aTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGa                                                                       >  2:1095193/1‑150 (MQ=255)
                                                                                cccATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGAttt                                                                    >  2:134342/1‑150 (MQ=255)
                                                                                cccATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACAGCTTTCTCTACAACGCGAACCAGCGTCCAGGAttt                                                                    >  2:634572/1‑150 (MQ=255)
                                                                                cccATATCGAGGGCGCGGCATTATAACACCGCTGCAAGTATATTTGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGATTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGAttt                                                                    <  1:622110/150‑1 (MQ=255)
                                                                                   atttCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGt                                                                 <  1:663360/147‑1 (MQ=255)
                                                                                   atatCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGt                                                                 >  2:428206/1‑150 (MQ=255)
                                                                                    tatCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTc                                                                >  2:440669/1‑150 (MQ=255)
                                                                                    tatCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTc                                                                >  1:368999/1‑150 (MQ=255)
                                                                                     atCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCt                                                               <  2:263013/150‑1 (MQ=255)
                                                                                         aGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGa                                                           <  1:319085/150‑1 (MQ=255)
                                                                                             cgcgGGATTATAACACCGATTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAAGACTAAATCCTGGAcgct                                                       <  2:973032/150‑4 (MQ=255)
                                                                                               cgGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGTACAGCgg                                                     >  1:707754/1‑150 (MQ=255)
                                                                                               cgGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCgg                                                     <  2:615156/150‑1 (MQ=255)
                                                                                               cgGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCgg                                                     >  1:713802/1‑150 (MQ=255)
                                                                                                   aTTATAACACCGCTTCATGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACgg                                                 <  2:255543/150‑1 (MQ=255)
                                                                                                      ataACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCAt                                              >  2:4065/1‑150 (MQ=255)
                                                                                                      ataACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGGTTTTTCGTATTGAGAGAGTGTACTGGATTACAGAACCGCTGTCTCTACAACGCGAACCAGCGTCCAGGATTTAGGCTGGGACAGCGGACGGCAt                                              >  2:165888/1‑150 (MQ=255)
                                                                                                           acCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTcgcg                                         <  2:966226/150‑1 (MQ=255)
                                                                                                           acCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTAATGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTcgcg                                         >  2:632806/1‑150 (MQ=255)
                                                                                                              gCTTCAAGGATATCGGTAGAAAAAATAAACGGCTCATATCTGAGCCGTTTATTAGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGAt                                      <  1:649234/150‑1 (MQ=255)
                                                                                                                        tatGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACg                            <  1:1257507/150‑1 (MQ=255)
                                                                                                                        tatGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACg                            >  1:1189207/1‑150 (MQ=255)
                                                                                                                          tGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTc                          <  1:427014/150‑1 (MQ=255)
                                                                                                                           gggTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCa                         <  2:1014453/150‑1 (MQ=255)
                                                                                                                            ggTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCAc                        >  2:1052154/1‑150 (MQ=255)
                                                                                                                            ggTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCAc                        <  1:288975/150‑1 (MQ=255)
                                                                                                                               aGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGa                     >  1:1316475/1‑150 (MQ=255)
                                                                                                                               aGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGa                     <  2:1049287/150‑1 (MQ=255)
                                                                                                                                taaaaaaTAAACGGCGCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGAt                    <  1:1132445/149‑1 (MQ=255)
                                                                                                                                 aaaaaaTAAACGGCTCATTTCTGAGCCGGTTAGTCGTATTGAGAGAGTGTACTGTATTACAGCACCGCTTTCTCTACAACGCGCACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCAGTCGCGGATTTGAGCCACGTCACCGaga                   >  2:345007/1‑148 (MQ=255)
                                                                                                                                 aaaaaaTAAACGGCTCATTTCTGAGCAGTTTATTCGAATTGAGACATTATACTGTATAACAGAACCGCTTTCTCTACAACGCGAACCAGAGTCCAGGATTTAGACTTGGACAGCCGACGGCAATAGCGGATTACAACCACGTCAcatata                   >  2:844117/1‑145 (MQ=255)
                                                                                                                                   cgaaTAAACGGCTCAGAAATGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATAcc                 >  2:250736/3‑150 (MQ=255)
                                                                                                                                   aaaaTAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATAcc                 >  2:772711/1‑150 (MQ=255)
                                                                                                                                        aaaCGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCAtt            >  1:1090965/1‑150 (MQ=255)
                                                                                                                                        aaaCGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCAtg            >  2:486429/1‑149 (MQ=255)
                                                                                                                                         aaCGGCTCAGAAATGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGCCGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTc           >  2:519859/1‑150 (MQ=255)
                                                                                                                                         aaCGGCTCAGAAATGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTc           >  1:236126/1‑150 (MQ=255)
                                                                                                                                         aaCGGCTCAGAAATGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCACCTTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCATCCACGTCCCCTCGACCGCATTc           >  1:104649/1‑150 (MQ=255)
                                                                                                                                           cGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgt         <  2:668023/150‑1 (MQ=255)
                                                                                                                                            ggCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgtt        <  1:1073670/150‑1 (MQ=255)
                                                                                                                                             gCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgttg       >  1:1155908/1‑150 (MQ=255)
                                                                                                                                             gCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgttg       >  1:1036423/1‑150 (MQ=255)
                                                                                                                                             gCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgttg       <  1:1265395/150‑1 (MQ=255)
                                                                                                                                             gCTCATTTCTGAGCCGTTTATTAGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTAAACCACGTCACCGATACCGCATTCgttg       >  1:1161200/1‑150 (MQ=255)
                                                                                                                                                cATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTtc    >  1:775324/1‑150 (MQ=255)
                                                                                                                                                  tttCTGAGCCGTTTATTCATATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTtctc  <  2:174235/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTTCTC  >  NC_000913/3448129‑3448424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: