Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,469,041 A→G 8.8% S271S (AGT→AGC chiA ← periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,469,0410AG 8.8% 204.3 / 5.4 80S271S (AGT→AGCchiAperiplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base A (27/44);  new base G (4/3);  total (31/49)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGA  >  NC_000913/3468894‑3469187
                                                                                                                                                   |                                                                                                                                                  
cGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACt                                                                                                                                                  <  1:971698/150‑1 (MQ=255)
  gAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCCCCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTgg                                                                                                                                                >  1:787700/1‑150 (MQ=255)
    gTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCa                                                                                                                                              >  1:20072/1‑150 (MQ=255)
      tGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATc                                                                                                                                            >  1:425244/1‑150 (MQ=255)
       ggggTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCg                                                                                                                                           <  2:1317060/150‑1 (MQ=255)
        gggTCACCGGTGTTTCCGGAGTTGGATCATCCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGGCAGGGTTACCATACTGGCTGATTTCAGTTGCCGTTGACGAGCGAACTTAACGCCATGGGTTACTGGCATCGt                                                                                                                                          >  1:2387/1‑150 (MQ=255)
        gggTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTAATATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGACATGGGTTACTGGCATCGt                                                                                                                                          >  2:612878/1‑150 (MQ=255)
           gcACCGGTGTTTCCGGAGTTGTAGCCACCGTACTAACAGCACCGCAATATTTATCCGGCTTAACTGAACAGGTTCCAGGGTTACCTTACTGTCTGATTTCTGGTGCGGTTGCGGCGCGATCATCACGCCATGGGTTACTGGCATCGtttt                                                                                                                                       <  2:1265203/149‑1 (MQ=255)
            cACCGGTGTTTCCGGAGTTGGATCAACCCGAGTCACAGCACCGACATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCAGACGGGCTGATTTCTGTTGCCGTTGACGCGCGATCATAACGCAATGTGTTACTGGATTCGTtttc                                                                                                                                      >  1:251842/1‑150 (MQ=255)
             aCCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct                                                                                                                                     >  1:1149687/1‑150 (MQ=255)
              ccGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCATGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGATCATAACGCCATGCGTTACTGGCATCGAtttctt                                                                                                                                    >  2:694323/1‑150 (MQ=255)
               cGGTGTTTCCTGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttcttt                                                                                                                                   <  2:414467/150‑1 (MQ=255)
                     ttCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATc                                                                                                                             <  2:269133/150‑1 (MQ=38)
                      gccGGAGGTGGAGCATCCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACACGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATACCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg                                                                                                                            <  2:255316/149‑1 (MQ=255)
                        cGGAGTTGGATCAACCGGAGTCACACCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGcc                                                                                                                          <  2:519549/150‑1 (MQ=255)
                         ggAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTTATTTTCGCATCGccc                                                                                                                         >  2:1238419/1‑149 (MQ=255)
                         ggAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCt                                                                                                                         >  1:620083/1‑150 (MQ=255)
                          gAGTTGGATCAACCGGAGTCACACCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACAAAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTg                                                                                                                        <  1:1136883/150‑1 (MQ=255)
                             tttcttCAACCGGAGTCACACCACCGCCATTATTATCCGGCTTACCGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTAGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCggac                                                                                                                     <  1:1238419/145‑3 (MQ=18)
                             ttGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGc                                                                                                                     <  2:1284084/150‑1 (MQ=255)
                               ggATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCCTCATTTTCTTTCGCATCCCCAGGACat                                                                                                                   >  2:983938/1‑149 (MQ=39)
                                 aTCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAAAAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATTGACTTTCGCATCGCCAGGCCAGtt                                                                                                                 >  1:452297/1‑150 (MQ=255)
                                   cAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTg                                                                                                               <  1:341781/150‑1 (MQ=255)
                                   cAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATTTAGTTTCGCATCACCGGGGCAATTTg                                                                                                               >  2:982622/1‑150 (MQ=38)
                                     aCCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGcc                                                                                                             <  1:1088541/150‑1 (MQ=255)
                                      ccGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTAGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCAGCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcg                                                                                                            <  2:1218939/150‑1 (MQ=255)
                                        ggAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg                                                                                                          <  1:752477/150‑1 (MQ=255)
                                         gAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCgcgc                                                                                                         >  1:1151348/1‑150 (MQ=255)
                                          aGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc                                                                                                        <  2:490950/150‑1 (MQ=255)
                                             cacaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATcat                                                                                                                                          <  2:247681/113‑3 (MQ=255)
                                             cacaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATcat                                                                                                                                          >  1:247681/1‑111 (MQ=255)
                                             cacaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAc                                                                                                     <  2:260700/150‑1 (MQ=255)
                                                aGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca                                                                                                  >  2:269847/1‑150 (MQ=255)
                                                aGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca                                                                                                  <  2:684751/150‑1 (MQ=255)
                                                 gCAACGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccac                                                                                                 <  2:197097/150‑1 (MQ=255)
                                                  cACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGGTACTGGCATCGTTTTCTTTCGAATCGCATGGGCAATTTGCGGCGCCTCCccacc                                                                                                >  2:811600/1‑150 (MQ=255)
                                                   aCCGCCATTATTATCCGGCTTACCGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGCGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTCCCGCGCCTAccccccc                                                                                               >  1:480547/1‑149 (MQ=255)
                                                   aCCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacca                                                                                               <  1:1074494/150‑1 (MQ=255)
                                                   aCCGCCATTATTATCCGGCATAACGGAACAGGAGCCAGGGGTACCATACTGGCTGATTTCTGTAGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacca                                                                                               <  1:34930/150‑1 (MQ=255)
                                                    ccGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCCCCAg                                                                                              >  1:1038487/1‑150 (MQ=255)
                                                     cGCCATTATTATCCGGCTTACCGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATTACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAgg                                                                                             <  2:515575/150‑1 (MQ=255)
                                                         attattATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTTGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGACTACCAACCAGGCGtt                                                                                         >  1:28411/1‑150 (MQ=255)
                                                           tattatCCCGCGTAACGGAAGAGGAGCCAGGGGTACCATACTGGCTGATTTCTGTGGCCGGTGCCGCGCGAACTTCTCGCCATGGGTTTCTGGCATCGTTTTCTATCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCgatgc                                                                                       <  1:333814/150‑5 (MQ=255)
                                                            attatCCGGCTTAACGGAACAGGAGCCATGGTTACCATACTGGCGGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCtt                                                                                      <  1:408900/150‑1 (MQ=255)
                                                            attatCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGAGTTCtt                                                                                      >  1:245799/1‑150 (MQ=255)
                                                              tatCCGGCTTAACGGAACAGGAGACAGGGATACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCTCATCGCCTGGGCATTTTGCCGCGCCTACCCACCAGGCGTTCTtat                                                                                    <  1:362869/150‑1 (MQ=255)
                                                               atCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGGATCGTTTTCTTTCGCATCGCCTGGGCAATTAGCCGCGCCTACCCACCAGGCGTTCTtata                                                                                   >  1:199046/1‑150 (MQ=255)
                                                               atCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCTTTTAATTTCGCATCACCAGGCCAATTTGAAGCGCCTACCCACCAGGCATTTTtata                                                                                   >  2:417312/1‑150 (MQ=25)
                                                               atCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTtata                                                                                   <  1:1127763/150‑1 (MQ=255)
                                                                tCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGATCATAACGCCATGGGTTACTGGCTTCGTTTTCTTTCGCTTCTCCGGGGCAAGTTGAAGCGCCTACACACCAGGCCTTAATATaa                                                                                  >  1:1042668/1‑150 (MQ=255)
                                                                 ccGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaaa                                                                                 >  1:1124388/1‑150 (MQ=255)
                                                                   ggCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATg                                                                               <  2:1054646/150‑1 (MQ=255)
                                                                          cGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt                                                                        >  1:1238796/1‑150 (MQ=255)
                                                                          cGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt                                                                        <  2:1131149/150‑1 (MQ=255)
                                                                                      cAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATTTTCTTTCGCATCTCCGGGCCAATTTGAAGAGGCTACCCACCAGGCATTTTAATAAAAGTAGCCATATAAGATAACGt                                                            >  1:44547/1‑150 (MQ=17)
                                                                                         ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc                                                         <  1:232772/150‑1 (MQ=255)
                                                                                             aCCATACTGGCTGATTTCTGTTGCGGTTGCCGCGCGATCATAACGCCATGGGTTACTGGCATCGTGTTCTTTCGCTTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGt                                                     <  1:1305503/150‑1 (MQ=255)
                                                                                              ccATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTAATTTCGCATCGCCAGGTCATTTTGCAGAGCCTACCCACCAGGCATTATTATATACCGCACCGTTATAGAGGACATACCAGGTa                                                    >  2:987282/1‑150 (MQ=255)
                                                                                              ccATACTGGATGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa                                                    <  1:645127/150‑1 (MQ=255)
                                                                                                aTACTGGCTGATTTCTGTTGCCTTTGCCGCGCGAACATAACGCCATTGGTTACTGGCATCGTTTTCTATCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTaga                                                  <  2:993264/150‑1 (MQ=255)
                                                                                                       cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCt                                           <  1:1118026/150‑1 (MQ=255)
                                                                                                       cTGATTTCTGTTGCAGTAGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTAATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCt                                           <  1:219773/150‑1 (MQ=255)
                                                                                                               tGTTGCCGTTGCCGCGCGAACTTTACTGAATTGGTTACTTTCAGAGTTTTCTTTCGCATAGCATTGGCAATTTGCCGCGCCTACACACCAGGAGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGAcc                                   <  1:73217/150‑1 (MQ=255)
                                                                                                                    ccgttgccgCGCGAACATAACGCCATTGGTTACTGGCATCGTGTTCTTTCGCATCGCCTGGGCAATTAGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTcc                              <  1:49432/150‑1 (MQ=255)
                                                                                                                    ccgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTcc                              >  2:426266/1‑150 (MQ=255)
                                                                                                                     cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCa                             <  2:686415/150‑1 (MQ=255)
                                                                                                                     cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCa                             <  2:20072/150‑1 (MQ=255)
                                                                                                                      gttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAg                            <  1:1148376/150‑1 (MQ=255)
                                                                                                                       ttgcggCCCGAACATCACGCCATGGTTTACTGGCATCGTTTTCTATCGCAGCGCCTGGGCAATTTGCCGCGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAgg                           <  2:942349/150‑1 (MQ=255)
                                                                                                                          ccgCGCGAACATAACGCCATGGGTTGCTGGCATCGTTTACTTTCGCATCCCCTGGGCAGTTTGCAGCGCCTACCCCCCAGGCGTTGTTATAAATGCCCCCCTTTTGGAGGACGGACCCTCTAGAAGACCCTTCATAACGTATGCAGGcta                        >  1:975234/1‑150 (MQ=16)
                                                                                                                                gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAg                  <  2:1060388/150‑1 (MQ=255)
                                                                                                                                gAACATAACGCCATCGTTTACTGTCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATTAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAg                  <  2:199046/150‑1 (MQ=255)
                                                                                                                                 aaCAAAACGACATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGc                 <  2:76299/150‑1 (MQ=255)
                                                                                                                                     tatcGCTATGGGGTTCTGGCATCTTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAg             <  1:1203414/147‑1 (MQ=255)
                                                                                                                                        cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTg          <  2:1124388/150‑1 (MQ=255)
                                                                                                                                        cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTg          <  2:401385/150‑1 (MQ=255)
                                                                                                                                           cATGGGCTACTGGCCTCCTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACTAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAACATGTAGAAGAACCTTCCTGACCTTTCAGGCTACTACATCATAGTTGTCGt      <  2:170596/150‑1 (MQ=255)
                                                                                                                                            aTGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGt      <  1:269847/150‑1 (MQ=255)
                                                                                                                                              gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGttt    <  2:436363/150‑1 (MQ=255)
                                                                                                                                                gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCGGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGa  >  1:968456/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGA  >  NC_000913/3468894‑3469187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: