Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,469,050 G→A 9.4% N268N (AAC→AAT chiA ← periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,469,0500GA 9.4% 195.5 / 3.8 76N268N (AAC→AATchiAperiplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base G (24/42);  new base A (5/2);  total (30/46)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTG  >  NC_000913/3468901‑3469198
                                                                                                                                                     |                                                                                                                                                    
ggggTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCg                                                                                                                                                      <  2:1317060/150‑1 (MQ=255)
 gggTCACCGGTGTTTCCGGAGTTGGATCATCCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGGCAGGGTTACCATACTGGCTGATTTCAGTTGCCGTTGACGAGCGAACTTAACGCCATGGGTTACTGGCATCGt                                                                                                                                                     >  1:2387/1‑150 (MQ=255)
 gggTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTAATATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGACATGGGTTACTGGCATCGt                                                                                                                                                     >  2:612878/1‑150 (MQ=255)
    gcACCGGTGTTTCCGGAGTTGTAGCCACCGTACTAACAGCACCGCAATATTTATCCGGCTTAACTGAACAGGTTCCAGGGTTACCTTACTGTCTGATTTCTGGTGCGGTTGCGGCGCGATCATCACGCCATGGGTTACTGGCATCGtttt                                                                                                                                                  <  2:1265203/149‑1 (MQ=255)
     cACCGGTGTTTCCGGAGTTGGATCAACCCGAGTCACAGCACCGACATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCAGACGGGCTGATTTCTGTTGCCGTTGACGCGCGATCATAACGCAATGTGTTACTGGATTCGTtttc                                                                                                                                                 >  1:251842/1‑150 (MQ=255)
      aCCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct                                                                                                                                                >  1:1149687/1‑150 (MQ=255)
       ccGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCATGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGATCATAACGCCATGCGTTACTGGCATCGAtttctt                                                                                                                                               >  2:694323/1‑150 (MQ=255)
        cGGTGTTTCCTGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttcttt                                                                                                                                              <  2:414467/150‑1 (MQ=255)
              ttCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATc                                                                                                                                        <  2:269133/150‑1 (MQ=38)
               gccGGAGGTGGAGCATCCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACACGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATACCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg                                                                                                                                       <  2:255316/149‑1 (MQ=255)
                 cGGAGTTGGATCAACCGGAGTCACACCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGcc                                                                                                                                     <  2:519549/150‑1 (MQ=255)
                  ggAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTTATTTTCGCATCGccc                                                                                                                                    >  2:1238419/1‑149 (MQ=255)
                  ggAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCt                                                                                                                                    >  1:620083/1‑150 (MQ=255)
                   gAGTTGGATCAACCGGAGTCACACCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACAAAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTg                                                                                                                                   <  1:1136883/150‑1 (MQ=255)
                      tttcttCAACCGGAGTCACACCACCGCCATTATTATCCGGCTTACCGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTAGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCggac                                                                                                                                <  1:1238419/145‑3 (MQ=18)
                      ttGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGc                                                                                                                                <  2:1284084/150‑1 (MQ=255)
                        ggATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCCTCATTTTCTTTCGCATCCCCAGGACat                                                                                                                              >  2:983938/1‑149 (MQ=39)
                          aTCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAAAAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATTGACTTTCGCATCGCCAGGCCAGtt                                                                                                                            >  1:452297/1‑150 (MQ=255)
                            cAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTg                                                                                                                          <  1:341781/150‑1 (MQ=255)
                            cAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATTTAGTTTCGCATCACCGGGGCAATTTg                                                                                                                          >  2:982622/1‑150 (MQ=38)
                              aCCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGcc                                                                                                                        <  1:1088541/150‑1 (MQ=255)
                               ccGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTAGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCAGCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcg                                                                                                                       <  2:1218939/150‑1 (MQ=255)
                                 ggAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg                                                                                                                     <  1:752477/150‑1 (MQ=255)
                                  gAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCgcgc                                                                                                                    >  1:1151348/1‑150 (MQ=255)
                                   aGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc                                                                                                                   <  2:490950/150‑1 (MQ=255)
                                      cacaGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAc                                                                                                                <  2:260700/150‑1 (MQ=255)
                                         aGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca                                                                                                             >  2:269847/1‑150 (MQ=255)
                                         aGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca                                                                                                             <  2:684751/150‑1 (MQ=255)
                                          gCAACGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccac                                                                                                            <  2:197097/150‑1 (MQ=255)
                                           cACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGGTACTGGCATCGTTTTCTTTCGAATCGCATGGGCAATTTGCGGCGCCTCCccacc                                                                                                           >  2:811600/1‑150 (MQ=255)
                                            aCCGCCATTATTATCCGGCTTACCGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGCGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTCCCGCGCCTAccccccc                                                                                                          >  1:480547/1‑149 (MQ=255)
                                            aCCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacca                                                                                                          <  1:1074494/150‑1 (MQ=255)
                                            aCCGCCATTATTATCCGGCATAACGGAACAGGAGCCAGGGGTACCATACTGGCTGATTTCTGTAGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacca                                                                                                          <  1:34930/150‑1 (MQ=255)
                                             ccGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCCCCAg                                                                                                         >  1:1038487/1‑150 (MQ=255)
                                              cGCCATTATTATCCGGCTTACCGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATTACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAgg                                                                                                        <  2:515575/150‑1 (MQ=255)
                                                  attattATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTTGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGACTACCAACCAGGCGtt                                                                                                    >  1:28411/1‑150 (MQ=255)
                                                    tattatCCCGCGTAACGGAAGAGGAGCCAGGGGTACCATACTGGCTGATTTCTGTGGCCGGTGCCGCGCGAACTTCTCGCCATGGGTTTCTGGCATCGTTTTCTATCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCgatgc                                                                                                  <  1:333814/150‑5 (MQ=255)
                                                     attatCCGGCTTAACGGAACAGGAGCCATGGTTACCATACTGGCGGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCtt                                                                                                 <  1:408900/150‑1 (MQ=255)
                                                     attatCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGAGTTCtt                                                                                                 >  1:245799/1‑150 (MQ=255)
                                                       tatCCGGCTTAACGGAACAGGAGACAGGGATACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCTCATCGCCTGGGCATTTTGCCGCGCCTACCCACCAGGCGTTCTtat                                                                                               <  1:362869/150‑1 (MQ=255)
                                                        atCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGGATCGTTTTCTTTCGCATCGCCTGGGCAATTAGCCGCGCCTACCCACCAGGCGTTCTtata                                                                                              >  1:199046/1‑150 (MQ=255)
                                                        atCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCTTTTAATTTCGCATCACCAGGCCAATTTGAAGCGCCTACCCACCAGGCATTTTtata                                                                                              >  2:417312/1‑150 (MQ=25)
                                                        atCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTtata                                                                                              <  1:1127763/150‑1 (MQ=255)
                                                         tCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGATCATAACGCCATGGGTTACTGGCTTCGTTTTCTTTCGCTTCTCCGGGGCAAGTTGAAGCGCCTACACACCAGGCCTTAATATaa                                                                                             >  1:1042668/1‑150 (MQ=255)
                                                          ccGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaaa                                                                                            >  1:1124388/1‑150 (MQ=255)
                                                            ggCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATg                                                                                          <  2:1054646/150‑1 (MQ=255)
                                                                   cGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt                                                                                   <  2:1131149/150‑1 (MQ=255)
                                                                   cGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt                                                                                   >  1:1238796/1‑150 (MQ=255)
                                                                               cAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATTTTCTTTCGCATCTCCGGGCCAATTTGAAGAGGCTACCCACCAGGCATTTTAATAAAAGTAGCCATATAAGATAACGt                                                                       >  1:44547/1‑150 (MQ=17)
                                                                                  ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc                                                                    <  1:232772/150‑1 (MQ=255)
                                                                                      aCCATACTGGCTGATTTCTGTTGCGGTTGCCGCGCGATCATAACGCCATGGGTTACTGGCATCGTGTTCTTTCGCTTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGt                                                                <  1:1305503/150‑1 (MQ=255)
                                                                                       ccATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTAATTTCGCATCGCCAGGTCATTTTGCAGAGCCTACCCACCAGGCATTATTATATACCGCACCGTTATAGAGGACATACCAGGTa                                                               >  2:987282/1‑150 (MQ=255)
                                                                                       ccATACTGGATGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa                                                               <  1:645127/150‑1 (MQ=255)
                                                                                         aTACTGGCTGATTTCTGTTGCCTTTGCCGCGCGAACATAACGCCATTGGTTACTGGCATCGTTTTCTATCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTaga                                                             <  2:993264/150‑1 (MQ=255)
                                                                                                cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCt                                                      <  1:1118026/150‑1 (MQ=255)
                                                                                                cTGATTTCTGTTGCAGTAGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTAATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCt                                                      <  1:219773/150‑1 (MQ=255)
                                                                                                        tGTTGCCGTTGCCGCGCGAACTTTACTGAATTGGTTACTTTCAGAGTTTTCTTTCGCATAGCATTGGCAATTTGCCGCGCCTACACACCAGGAGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGAcc                                              <  1:73217/150‑1 (MQ=255)
                                                                                                             ccgttgccgCGCGAACATAACGCCATTGGTTACTGGCATCGTGTTCTTTCGCATCGCCTGGGCAATTAGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTcc                                         <  1:49432/150‑1 (MQ=255)
                                                                                                             ccgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTcc                                         >  2:426266/1‑150 (MQ=255)
                                                                                                              cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCa                                        <  2:686415/150‑1 (MQ=255)
                                                                                                              cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCa                                        <  2:20072/150‑1 (MQ=255)
                                                                                                               gttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAg                                       <  1:1148376/150‑1 (MQ=255)
                                                                                                                ttgcggCCCGAACATCACGCCATGGTTTACTGGCATCGTTTTCTATCGCAGCGCCTGGGCAATTTGCCGCGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAgg                                      <  2:942349/150‑1 (MQ=255)
                                                                                                                   ccgCGCGAACATAACGCCATGGGTTGCTGGCATCGTTTACTTTCGCATCCCCTGGGCAGTTTGCAGCGCCTACCCCCCAGGCGTTGTTATAAATGCCCCCCTTTTGGAGGACGGACCCTCTAGAAGACCCTTCATAACGTATGCAGGcta                                   >  1:975234/1‑150 (MQ=16)
                                                                                                                         gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAg                             <  2:1060388/150‑1 (MQ=255)
                                                                                                                         gAACATAACGCCATCGTTTACTGTCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATTAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAg                             <  2:199046/150‑1 (MQ=255)
                                                                                                                          aaCAAAACGACATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGc                            <  2:76299/150‑1 (MQ=255)
                                                                                                                              tatcGCTATGGGGTTCTGGCATCTTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAg                        <  1:1203414/147‑1 (MQ=255)
                                                                                                                                 cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTg                     <  2:1124388/150‑1 (MQ=255)
                                                                                                                                 cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTg                     <  2:401385/150‑1 (MQ=255)
                                                                                                                                    cATGGGCTACTGGCCTCCTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACTAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAACATGTAGAAGAACCTTCCTGACCTTTCAGGCTACTACATCATAGTTGTCGt                 <  2:170596/150‑1 (MQ=255)
                                                                                                                                     aTGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGt                 <  1:269847/150‑1 (MQ=255)
                                                                                                                                       gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGttt               <  2:436363/150‑1 (MQ=255)
                                                                                                                                         gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCGGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGa             >  1:968456/1‑150 (MQ=255)
                                                                                                                                             cTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATTATAAATGCCGCCGTTATAGCTAACCTAACATGGAGAACACCCGACCAGAGAATTCCATGCTACTACAGAATATTTTTAGGTTTACTTa         >  2:631922/1‑150 (MQ=255)
                                                                                                                                                   tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCt   >  2:1111734/1‑150 (MQ=255)
                                                                                                                                                    cGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTTATAAAGGCCACCGTTATAGAGGACATACCAGGTATGACAACCGTCCTGACGATTCCAGGCTAAATCACCAGAGTAGTCTTGTGACTTaggggatg  >  2:219453/1‑143 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTG  >  NC_000913/3468901‑3469198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: