Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,471,366 A→G 8.5% intergenic (‑37/+34) tufA ← / ← fusA translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,3660AG 8.5% 227.6 / 11.2 84intergenic (‑37/+34)tufA/fusAtranslation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base A (41/35);  new base G (4/3);  total (45/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.18e-01

TAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAG  >  NC_000913/3471218‑3471512
                                                                                                                                                    |                                                                                                                                                  
tAGCCAGTACGGTGGTGATTGCGGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                                                                                                                   >  1:1206494/1‑150 (MQ=255)
   ccAGTACGGTGGTGATTGCAGCGGTCAGTGTAGTTTTACCGTGGGCAACGTGGCCGATAGTACCAACGTTTACGTGAGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagaga                                                                                                                                                <  2:558884/150‑1 (MQ=255)
   ccAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagaga                                                                                                                                                <  1:1047649/150‑1 (MQ=255)
   ccAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGATCAAATTTTTCTTTAGACACGCATAAATTCATTAATATAGTCATCTCCCCTTCATgagaga                                                                                                                                                >  1:167709/1‑150 (MQ=18)
    cAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                                                                               <  2:833709/150‑1 (MQ=255)
     attACGGTGGTTATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTTGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                                                                              <  2:561534/148‑1 (MQ=21)
      gTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                                                                                                             >  1:186904/1‑150 (MQ=255)
      ctACGGGGGTGAATTCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATCTTCCTTACAATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                                                                                                             <  2:746365/149‑1 (MQ=25)
       tACGGTGGTGATTGCAGCGGTGAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                                                                            <  1:12665/150‑1 (MQ=21)
         cGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCGGGAGAGAGCACgg                                                                                                                                          <  2:23034/150‑1 (MQ=255)
         cGGTGGTGATTGCAGCGGTCAGAGTAGGTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                                                                          <  1:667894/150‑1 (MQ=255)
         cGGGGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                                                                          <  1:1113679/150‑1 (MQ=255)
          ggtggtGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACggg                                                                                                                                         >  2:470963/1‑150 (MQ=255)
            gggtgATTGCAGCGGCCAGAGTAGTTTTACCGTGGTCAACGTGGAATATATTACCTACGTTAACGTGATGTTTTGTACGTTTAAATTTTTCTTTAGACTCGGCTATATTCATTACTATAGTGCTCTATCCTGAAGGGGAGAGCActatag                                                                                                                                       <  2:61384/149‑6 (MQ=255)
             ggtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGCCt                                                                                                                                      >  2:1033425/1‑150 (MQ=255)
             ggtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACt                                                                                                                                      >  1:458997/1‑150 (MQ=255)
              gtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAAGGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTAAAATTTTTCTTTAGACACGGCTATATTCCTACTTTAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                                                                    <  2:558702/150‑1 (MQ=255)
              gtgATTGCAGCGGTACGATGAGTTTAACCGTTGGCAACGTGGCCGATAGTACCAACGTTACCGTGCGGTTTTGTACGGGCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACtt                                                                                                                                     <  1:246284/150‑1 (MQ=255)
                   tGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                                                                                                                >  1:370623/1‑150 (MQ=255)
                     cAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                                                                              >  2:778185/1‑150 (MQ=255)
                      aGCGGTAAGAGTAGTTTTACCGTGGTCAACGTGGCAGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGAGACGGCTTTATTACTGACTATAGTGCTCTCCCATTCAGGAGAGAGAACGGGACTTTGGAATTa                                                                                                                             >  2:194394/1‑150 (MQ=25)
                         ggTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                                                                          <  2:534837/150‑1 (MQ=255)
                         ggTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                                                                          <  1:1246097/150‑1 (MQ=255)
                             agagTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTa                                                                                                                      >  1:650861/1‑150 (MQ=255)
                              gagTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAg                                                                                                                     <  2:751130/150‑1 (MQ=255)
                                 tagtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCt                                                                                                                  <  1:557677/150‑1 (MQ=255)
                                  agtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCtt                                                                                                                 >  1:865468/1‑150 (MQ=255)
                                  agtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGAACGGGACTTTGGTATTAACCCTTAGGCtt                                                                                                                 >  1:908034/1‑150 (MQ=255)
                                      ttACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAttt                                                                                                             <  1:1211489/150‑1 (MQ=255)
                                             ggTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                                                                                      >  2:468663/1‑150 (MQ=255)
                                              gtaaaCGTGGCCTATAGTACCAACTTTAAGGTGGGGTTTTTTATGTTCAAATTTTTATTTAGACACGGTTATATTCCTTATTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACggg                                                                                                     <  2:1174661/147‑1 (MQ=255)
                                              gTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACggg                                                                                                     >  1:1164752/1‑150 (MQ=255)
                                               tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                                                                    >  2:1035650/1‑150 (MQ=255)
                                               tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                                                                    <  2:723975/150‑1 (MQ=255)
                                               tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                                                                    >  2:1178116/1‑150 (MQ=255)
                                                cAACGTGTCCGATAGTACCAACTTTAACGGGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCt                                                                                                   <  1:102687/150‑1 (MQ=255)
                                                  aCGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATAATCCTTACTATAGTGCTCTCCCCTTCAGTTGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTc                                                                                                 <  2:1127744/150‑1 (MQ=255)
                                                   cGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCa                                                                                                <  2:357418/150‑1 (MQ=255)
                                                     tGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGATCTCCCCTTCAATAGAGAGCACGGGACTTTGGTATTAAACATTAGGCTTATTTACCACGTGCTTCaaa                                                                                              >  1:249621/1‑149 (MQ=37)
                                                       gCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTACCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTa                                                                                            >  2:989227/1‑150 (MQ=255)
                                                       gCCGAAAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGAGCTCTCCACTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTa                                                                                            >  1:97715/1‑150 (MQ=255)
                                                        ccGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTAc                                                                                           >  1:562946/1‑150 (MQ=255)
                                                        ccGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTAc                                                                                           >  1:1096772/1‑150 (MQ=255)
                                                           aTAGTACCAACGTTAACGGGCGGTTTTGTCCGTTCAAATTTTTCTTTAGTCACGGCTATATTCCTTACTATAGTGCGCTCCCCATCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                                                                                        <  1:505691/150‑1 (MQ=255)
                                                            tattACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGAGACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                                                                                       <  1:931768/147‑1 (MQ=255)
                                                             aGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCt                                                                                      >  2:289081/1‑150 (MQ=255)
                                                                aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                                                                                   >  2:1210561/1‑150 (MQ=255)
                                                                  cAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCa                                                                                 >  2:272449/1‑150 (MQ=255)
                                                                  cAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCa                                                                                 >  1:493685/1‑150 (MQ=255)
                                                                   aaCGTAAACGTGGGGTTTTGTACGTTCAAATTTTTCTTTATACACGGTTAAATTCCTTACTATTGTGCGCTCCCCTTCCGTAGTGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCaa                                                                                <  2:966771/150‑1 (MQ=255)
                                                                    aCGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAAc                                                                               >  1:1201361/1‑150 (MQ=255)
                                                                     cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACg                                                                              >  2:290368/1‑150 (MQ=255)
                                                                     cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACg                                                                              >  2:879418/1‑150 (MQ=255)
                                                                     cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGAACGGGACTTTTGTATTAACCCTCAGGCTTATTTACAACGGGCTTCAATTACGGCCTGAGCAACg                                                                              >  1:878477/1‑150 (MQ=255)
                                                                          aCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTAATATAGTGATCTCCCCTTAAGGAGAGATCTCGGGAATTTGCTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACt                                                                         >  2:719055/1‑150 (MQ=255)
                                                                           cGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCATGAGCAACGTTACTc                                                                        >  1:696323/1‑150 (MQ=255)
                                                                                 gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt                                                                  >  1:565156/1‑150 (MQ=255)
                                                                                  ggttGTACGTTAAAATTTTATTTTATACACGTATATATTCATTACTATACTGCTATGCACTTCAGTAGAGAGCACGTGACTTTGGTATTACCCCTTAGGCTTATTTACCAGGGGCTTAATTTAGTGCCTGAGCAACGTTTATCTGCGCtt                                                                 <  2:159044/148‑1 (MQ=255)
                                                                                    ttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca                                                               <  1:1141847/150‑1 (MQ=255)
                                                                                    ttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca                                                               <  1:854598/150‑1 (MQ=255)
                                                                                     tGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCACCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcat                                                              >  1:1272528/1‑150 (MQ=255)
                                                                                         cGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTTTTTACAACGGGCTTCAATTACGGCCTGAGAAACGTTACTAGGCGCTTCATCATa                                                          >  1:1287717/1‑150 (MQ=255)
                                                                                               aaTTTTTCTTTATACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAg                                                    <  1:282749/150‑1 (MQ=255)
                                                                                                  ttttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGaa                                                 <  2:370623/150‑1 (MQ=255)
                                                                                                     tCTTTAGACACGGCTATATTCCTTACTATAGTGCTCACCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTc                                              <  1:301672/150‑1 (MQ=255)
                                                                                                      cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTcc                                             <  2:186904/150‑1 (MQ=255)
                                                                                                      cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTcc                                             >  1:340464/1‑150 (MQ=255)
                                                                                                      cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTcc                                             >  1:1304708/1‑150 (MQ=255)
                                                                                                      cTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTcc                                             >  1:395139/1‑150 (MQ=255)
                                                                                                         tAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATa                                          >  1:553740/1‑150 (MQ=255)
                                                                                                          aGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAg                                         >  2:1081090/1‑150 (MQ=255)
                                                                                                            acacGGATATATTCCTTACTATCGGGCTCTCCCCATCTGGAGAGAGAACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAgtg                                       <  1:1144934/150‑1 (MQ=255)
                                                                                                             cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGCTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTGACTCGGCGCTTCATCAGACGTCAGGAATTCCATTgtgt                                      >  1:70413/1‑150 (MQ=255)
                                                                                                               cGGCTATATTCCTTCCTATAGTGGTCTCCCCTTCTGGAGCGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTat                                    <  2:1128388/150‑1 (MQ=255)
                                                                                                               cGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACACTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTat                                    >  1:162318/1‑150 (MQ=255)
                                                                                                                  cTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTTCCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTGAGGAATTCCATAGTGTatgat                                 >  1:471606/1‑150 (MQ=255)
                                                                                                                   tataTTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGTGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTatgatg                                <  2:167709/150‑1 (MQ=255)
                                                                                                                     tatTCCTTACTATAGTGCTCTCCCGTTCATGAGAGAGCACGGGACTTTGGTATTAACCCTTATGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATTATGCa                              <  1:736782/150‑1 (MQ=255)
                                                                                                                               tcccgTGCTCTCTCCTGATGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATGATACGTCAGGAATTCCATAGTGTATGATGCGCGACCTTggg                    >  1:69216/5‑150 (MQ=255)
                                                                                                                                ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGt                   <  1:190310/150‑1 (MQ=255)
                                                                                                                                 ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc                  >  2:485114/3‑150 (MQ=255)
                                                                                                                                  cgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGGGTATGATGCACGACCTTTGGTCa                 >  2:310787/2‑150 (MQ=255)
                                                                                                                                    tGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCaga               >  1:12039/1‑150 (MQ=255)
                                                                                                                                    ggcgccccccTTCCGTAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCaga               <  1:961356/144‑1 (MQ=255)
                                                                                                                                    ggCTCTCCCCGTCCGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCaga               <  2:190214/149‑1 (MQ=255)
                                                                                                                                     gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCagag              >  2:2515/1‑150 (MQ=255)
                                                                                                                                      ctctCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAgaga             <  2:370945/150‑1 (MQ=255)
                                                                                                                                        ctcCCCTTCACGATAGAGCACGGGACTTTTGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAAc           <  2:1061602/150‑1 (MQ=255)
                                                                                                                                                 aGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAg  <  2:1055123/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAG  >  NC_000913/3471218‑3471512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: