Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,483,244 A→G 7.7% S15G (AGT→GGT) ‡ yheT → UPF0017 family putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,483,2440AG 7.7% 220.0 / 8.6 78S15G (AGT→GGT) ‡yheTUPF0017 family putative hydrolase
Reads supporting (aligned to +/- strand):  ref base A (39/33);  new base G (3/3);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

CTGCAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTG  >  NC_000913/3483095‑3483391
                                                                                                                                                     |                                                                                                                                                   
ctgcAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTcagcagca                                                                                                                                                     >  1:757476/1‑150 (MQ=255)
  gcAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGt                                                                                                                                                   >  2:216037/1‑150 (MQ=255)
         cAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAAt                                                                                                                                            <  2:757476/150‑1 (MQ=255)
         cAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCACTGCTGCTGAAt                                                                                                                                            <  2:1137484/150‑1 (MQ=255)
           aGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTc                                                                                                                                          >  2:1141095/1‑150 (MQ=255)
           aGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACAGATGGCGCAGATACCGCCGCCAGCTGCCAATGAATTCAGCCGCAGTGCTGAAATc                                                                                                                                          >  2:98708/1‑150 (MQ=255)
              cAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCACTGCTGCTGAATTCAtt                                                                                                                                       <  1:537177/150‑2 (MQ=255)
               aGCGCCAAATCCGGCCTGTAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGTAGCAGCTTGAGCAGATGCTGCTGGAAGGACAAAGCAACTGATGGCGCAGATAACGACGACAGATGCCAATGAATTCAGCACTGCTGCTGAATTCAttg                                                                                                                                      <  2:860155/150‑3 (MQ=255)
                gcgcCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATccc                                                                                                                                     <  1:29114/150‑1 (MQ=255)
                 cgcCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATcccc                                                                                                                                    >  2:345928/1‑150 (MQ=255)
                 cgcCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATcccc                                                                                                                                    >  2:685520/1‑150 (MQ=255)
                  gcCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCa                                                                                                                                   <  1:732690/150‑1 (MQ=255)
                   ccAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCAt                                                                                                                                  >  2:417379/1‑150 (MQ=255)
                    cAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTTGACGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTCATGGCGCTGAAAACGACGCCCGATGCCAATGAATTCCGCAGCAGTGCTGAATTCATCCCCATg                                                                                                                                 >  2:1057180/1‑150 (MQ=255)
                         ccGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGCTGCTGCTGGAAGGCCAAAGCAACTGATGGCGAAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCgg                                                                                                                            >  1:690807/1‑150 (MQ=255)
                               tGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAg                                                                                                                      >  1:742971/1‑150 (MQ=255)
                               tGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAg                                                                                                                      <  2:378657/150‑1 (MQ=255)
                                   agagTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAATGCAACTGATGGCGCAGATAACGACGCCCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAAt                                                                                                                  >  2:227655/1‑150 (MQ=255)
                                    gagTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAAtt                                                                                                                 >  1:406504/1‑150 (MQ=255)
                                        gCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCa                                                                                                             >  1:1015834/1‑150 (MQ=255)
                                        gCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCa                                                                                                             <  1:539984/150‑1 (MQ=255)
                                        gCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGAAGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCa                                                                                                             <  1:845922/150‑1 (MQ=255)
                                         cGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGAAGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCAt                                                                                                            >  2:673293/1‑150 (MQ=255)
                                            actggcatggcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGACAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTg                                                                                                         <  2:790141/148‑1 (MQ=255)
                                            aaTGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGATGGAAGGCCAAAGCAACGGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGATTTCATCCCCCTGCGCGGCTTTAGCAATTGTCATCTg                                                                                                         >  1:1177537/1‑150 (MQ=255)
                                                gcatggcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCaaa                                                                                                     >  2:1176194/1‑150 (MQ=255)
                                                 catggcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAAc                                                                                                    <  2:848621/150‑1 (MQ=255)
                                                      cTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCAtgc                                                                                               >  1:682266/1‑150 (MQ=255)
                                                       tGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCAtgct                                                                                              <  1:1154281/150‑1 (MQ=255)
                                                           atcccAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAAGTGATGGCGCAGATAACGACGACCGATGCCAATGATTTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCcg                                                                                          <  1:675987/148‑1 (MQ=255)
                                                             cccAGGAGCAGCTTGAGAAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCGGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCcgcg                                                                                        <  1:605130/150‑1 (MQ=255)
                                                              ccAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGCTGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGGCGCGt                                                                                       >  1:188112/1‑150 (MQ=255)
                                                              ccAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGt                                                                                       <  1:474686/150‑1 (MQ=255)
                                                                    gcagcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGttt                                                                                 <  2:336539/150‑1 (MQ=255)
                                                                    gcagcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGttt                                                                                 <  1:798138/150‑1 (MQ=255)
                                                                     cagcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtc                                                                                >  1:752251/1‑150 (MQ=255)
                                                                      agcTTGAGCAGATGCTGCTGGATGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCCGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtcg                                                                               >  1:184483/1‑150 (MQ=255)
                                                                       gcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtcgt                                                                              >  1:268621/1‑150 (MQ=255)
                                                                       gcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtcgt                                                                              >  2:654857/1‑150 (MQ=255)
                                                                        cTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtcgtc                                                                             >  2:893193/1‑150 (MQ=255)
                                                                          tGAGCAGATGCTGCTGGAAGGCAAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGACATCTGCAAACCATGCAGCCGCGTCTGTTTcgtcgt                                                                           >  1:731626/1‑150 (MQ=255)
                                                                             gCAGTTGCTGCGGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAg                                                                        <  1:95181/150‑1 (MQ=255)
                                                                             acAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAAATCAGCAGCAGTGCGGATATCATACCCATGCGCGGCTATAGAAAATGTCATCTGCAAACCATGCTGCCGGGTCTGTTAGGTCGTCAg                                                                        >  2:702462/2‑150 (MQ=255)
                                                                                gATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCAGGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTg                                                                     <  1:763440/150‑1 (MQ=255)
                                                                                 aTGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGa                                                                    <  2:712399/150‑1 (MQ=255)
                                                                                      tctgGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAAGCATGGTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTc                                                               <  2:534233/149‑1 (MQ=255)
                                                                                      gctgGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGGCTGTTTCGTCGTCAGGTGAAATTc                                                               <  2:316876/150‑1 (MQ=255)
                                                                                         ggAAGGCCAAAGCAACTGATGGCGCAGATAACGACGCCCGATGCCAATGAATTCAGCAGCAGTGCTGAAGTCATCCGCATGCGCGGCTTTAGCTATTGTGATGGGCACACCATGCTGCCGCGTCTGTTTCGTCGTGCGGTGAAATTCAcc                                                            >  2:822280/1‑150 (MQ=255)
                                                                                         ggAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAcc                                                            <  1:756616/150‑1 (MQ=255)
                                                                                          gAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAccc                                                           <  2:1051884/150‑1 (MQ=255)
                                                                                           aaGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAcccc                                                          <  2:468859/150‑1 (MQ=255)
                                                                                             ggCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGt                                                        >  1:1129542/1‑150 (MQ=255)
                                                                                              gCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTa                                                       >  2:301679/1‑150 (MQ=255)
                                                                                              gCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTa                                                       >  2:874935/1‑150 (MQ=255)
                                                                                                cAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTGTCGTCGTCAGGTGAAATTCACCCCGTACt                                                     >  2:801258/1‑150 (MQ=255)
                                                                                                   aGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGc                                                  <  2:394106/150‑1 (MQ=255)
                                                                                                   aGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATACCCATGCGCGGCTTTAGCAATTGTCATCTGCACACAATGCTGCCGCGACTGTTTCGTCGTCAGGTGAAATTCACCCATTACTGGc                                                  >  2:738390/1‑150 (MQ=255)
                                                                                                       aCTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCg                                              <  1:448762/150‑1 (MQ=255)
                                                                                                            tGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTgg                                         <  2:28304/150‑1 (MQ=255)
                                                                                                              gcgcAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAg                                       >  2:159797/1‑150 (MQ=255)
                                                                                                               cgcAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCCCCCCGTACTGGCAGCGGCTGGAGt                                      >  2:716923/1‑150 (MQ=255)
                                                                                                               cgcAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGAGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATCCTGCCCCGGCGGTTTCGGGGTCAGGTGAAATTACCCCCGGAGAGGCAGCGGCTGgtgt                                      >  1:1263474/1‑150 (MQ=255)
                                                                                                                  aGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGc                                   <  2:520864/150‑1 (MQ=255)
                                                                                                                   caatacgacgACCGAAGCCAATGAATTCCGCCGCAGTGCTGAATTCATCCCCATGCGCGGCTGTAGCAATTGTCATCTGCAACCCATGCTGCCGCGTCTGCTTCGTCGTCAGGTGAAATTCACCCCGGACTGGCAGCGGCTGGAGTTGcc                                  <  1:862027/146‑1 (MQ=255)
                                                                                                                     tAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCg                                <  2:174268/150‑1 (MQ=255)
                                                                                                                           cgacCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCg                          <  1:267678/150‑1 (MQ=255)
                                                                                                                                  tGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGt                   <  1:646399/150‑1 (MQ=255)
                                                                                                                                   gCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTc                  <  1:866475/150‑1 (MQ=255)
                                                                                                                                    ccAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGGTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGAATTTGTCg                 >  2:301277/1‑150 (MQ=255)
                                                                                                                                    ccAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCg                 >  1:10327/1‑150 (MQ=255)
                                                                                                                                     ggaTGAATTCAGCACTGCTGCTGAATTCATTCCCATGCGCGGCTTTAGCTATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGa                >  1:688181/3‑150 (MQ=255)
                                                                                                                                     cAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACACCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGa                >  2:886552/1‑150 (MQ=255)
                                                                                                                                      gaTGAATTCAGCTCTGCTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGAt               >  1:626551/2‑150 (MQ=255)
                                                                                                                                      aaTGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGAt               <  1:801258/150‑1 (MQ=255)
                                                                                                                                        tGAATTCAGCACTGCTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCt             >  1:1165737/1‑150 (MQ=255)
                                                                                                                                          aaTTCAGCAGCAGTGCTGAATTCATCCCCAAGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTg           >  2:646780/1‑150 (MQ=255)
                                                                                                                                           aTTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGc          <  1:1240315/150‑1 (MQ=255)
                                                                                                                                            ttCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGTCTGGAGTTGCCCGACGGCGATTTTGTGGATCTTGCg         >  2:346190/1‑150 (MQ=255)
                                                                                                                                            ttCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGCTGAAATTCACCCCGTACTGGCTGCGGCGGGAGTTGCCCGACGGCCATATTGTCGATCATGCg         >  2:606602/1‑150 (MQ=255)
                                                                                                                                              cagcagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTg       <  1:1230791/150‑1 (MQ=255)
                                                                                                                                              cagcagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTg       <  1:654857/150‑1 (MQ=255)
                                                                                                                                              aagcatcagTGCTGAATTCATCCCCATGCGCGTCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGGGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCTATCTTGCGTg       <  1:622946/149‑1 (MQ=255)
                                                                                                                                               agcagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTgg      <  1:685520/150‑1 (MQ=255)
                                                                                                                                                 cagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAg    >  2:1289558/1‑150 (MQ=255)
                                                                                                                                                   gcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTg  <  1:93092/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTGCAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTG  >  NC_000913/3483095‑3483391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: