Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,488,983 C→A 7.7% V400L (GTG→TTG)  argD ← bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,488,9830CA 7.7% 241.1 / 7.5 87V400L (GTG→TTG) argDbifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (33/47);  new base A (2/5);  total (35/52)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAGCGACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGT  >  NC_000913/3488835‑3489132
                                                                                                                                                    |                                                                                                                                                     
gTCAGAGAACGCTGCACGGGATTTGGCTGAGTCGCAAGTTGCGGTATTCGAATGCGTAATGCAGGCTAAACCCTCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCACAACACCTTCGCCACcg                                                                                                                                                     <  2:1021297/150‑1 (MQ=255)
 tCAGCGACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACcg                                                                                                                                                     <  1:1304944/150‑1 (MQ=255)
     cGACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTg                                                                                                                                                 >  2:1270685/1‑150 (MQ=255)
       aCCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTggg                                                                                                                                               >  1:572606/1‑150 (MQ=255)
         cGCATCACGGGATTTGGCTGAGTCTCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCAACGGATAAGGCATTCACGCCGCATCCGACAGCTTTTGCCGGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCg                                                                                                                                             <  1:150398/150‑1 (MQ=255)
         cGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCg                                                                                                                                             <  2:136007/150‑1 (MQ=255)
         cGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCAATCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCg                                                                                                                                             <  2:1166908/150‑1 (MQ=255)
             tCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaagg                                                                                                                                         <  1:1048589/150‑3 (MQ=255)
             tCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTGTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaagg                                                                                                                                         <  1:667750/150‑3 (MQ=255)
              cACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCCTCCGACATCTTTTGCCTGATGCTTCGGATTTACGCCCCAGCCACCTTCGCCACCGCGTGGGCGAAACg                                                                                                                                        >  1:237107/1‑150 (MQ=255)
              cACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaaggt                                                                                                                                        <  1:178944/150‑4 (MQ=255)
               aCGGTATTTGGCTGAGTCGCAAGTTGCGGGATTCGAATGCGTAATGCATGCTAAACCATCGGATAAGTCATTCACGCCGCATACGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaaggtg                                                                                                                                       <  1:653899/150‑5 (MQ=255)
               aCGGGATTTGGCTGAGTCGCAAGGTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGt                                                                                                                                       <  1:506129/150‑1 (MQ=255)
                cGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaaggtgg                                                                                                                                      <  1:1031760/150‑6 (MQ=255)
                cGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCCAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGACTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGtt                                                                                                                                      >  2:16898/1‑150 (MQ=255)
                  ggAATTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTACGCCACCGCGTGGGCGAAACGTTGc                                                                                                                                    <  2:1065391/150‑1 (MQ=255)
                     tttGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATc                                                                                                                                 <  1:1288458/150‑1 (MQ=255)
                      ttGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATcc                                                                                                                                <  1:1192571/150‑1 (MQ=255)
                      ttGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATcc                                                                                                                                <  2:780505/150‑1 (MQ=255)
                          ctgcggcGCACGGTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTc                                                                                                                            <  1:788876/144‑1 (MQ=255)
                          cTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTc                                                                                                                            >  1:1190843/1‑150 (MQ=255)
                           tGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCa                                                                                                                           <  2:58788/150‑1 (MQ=255)
                            gAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCAt                                                                                                                          <  1:972953/150‑1 (MQ=255)
                             aGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATc                                                                                                                         <  1:329838/150‑1 (MQ=255)
                               tCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGa                                                                                                                       <  1:919894/150‑1 (MQ=255)
                                cGCAAGTAGCGGGATACAAACGAGAAATGCAGGCAAAACCATCCGATAACGCATACACGCCGCATCCGACATCTTTTGCCAGATGCTTCGTATTTACGCACCAAACACCATCGCCCCCGCGTGGGCAAAACGTTGCATCCCTTCATCGat                                                                                                                      >  2:1131480/1‑150 (MQ=255)
                                  cAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGatat                                                                                                                    <  1:802888/150‑1 (MQ=255)
                                  cAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGatat                                                                                                                    <  1:450800/150‑1 (MQ=255)
                                  cAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGACTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGCAACGTTGCATCCCTTCATCGatat                                                                                                                    >  2:1046510/1‑150 (MQ=255)
                                    aGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATcc                                                                                                                  <  1:40558/150‑1 (MQ=255)
                                    aGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATcc                                                                                                                  >  1:556369/1‑150 (MQ=255)
                                            gATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCATATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTc                                                                                                          <  2:509038/150‑1 (MQ=255)
                                                   aGGCGTAATTCAGGCTAAACCTTCGGATAATGCATTCACGCCGCATCCGACATCTTTTGCCTGATGGTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTccaccacc                                                                                                   <  2:368290/150‑1 (MQ=255)
                                                     gCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAg                                                                                                 <  1:1247061/150‑1 (MQ=255)
                                                          aTGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGCCg                                                                                            >  2:470493/1‑150 (MQ=255)
                                                            gCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGt                                                                                          >  2:277299/1‑150 (MQ=255)
                                                              aGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGc                                                                                        >  1:9567/1‑150 (MQ=255)
                                                                 cTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCaaa                                                                                     >  1:109160/1‑150 (MQ=255)
                                                                   aaaCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCACCCCCCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATATTCCACCACCAGCGACGGTGCAAAAc                                                                                   >  1:1042231/1‑150 (MQ=255)
                                                                     gccATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGCAGGTGCAAAACGc                                                                                 >  1:1082802/2‑150 (MQ=255)
                                                                      ccATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                                <  2:572476/150‑1 (MQ=255)
                                                                      ccATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                                <  1:1152639/150‑1 (MQ=255)
                                                                         tCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCCCCACCAGCGACGGTGCAAAACGCATca                                                                             >  1:31689/1‑150 (MQ=255)
                                                                          cGGATAAGGCATTCACGCCGCATCCGACATCTTATGCCTGATGCTAATTATTTACTCCCCAACAACCTTCGCCACCGCGTGGGCGAAACGTTGCATACCTTCATCGATATCCGCATCTTCCACCACCACAGACCGTGCAAAACGCATccc                                                                            >  1:647135/1‑148 (MQ=255)
                                                                             aTAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtc                                                                         <  2:340736/150‑1 (MQ=255)
                                                                              tAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCATATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtcc                                                                        <  1:508836/150‑1 (MQ=255)
                                                                              tAAGGCAATCACGCCGCATCCGACATCTTTAGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGAAGGTGCAAAACGCATCACAtcc                                                                        <  2:569118/150‑1 (MQ=255)
                                                                               aaGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtccg                                                                       <  1:1285473/150‑1 (MQ=255)
                                                                               aaGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtccg                                                                       <  1:396343/150‑1 (MQ=255)
                                                                                aGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtccgg                                                                      <  1:804942/150‑1 (MQ=255)
                                                                                aGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtccgg                                                                      >  1:607823/1‑150 (MQ=255)
                                                                                aGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtccgg                                                                      >  2:971381/1‑150 (MQ=255)
                                                                                      tCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGc                                                                <  1:468830/150‑1 (MQ=255)
                                                                                       cACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCa                                                               >  1:522881/1‑150 (MQ=255)
                                                                                        aCGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCAt                                                              <  2:9567/150‑1 (MQ=255)
                                                                                          gccgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTc                                                            <  1:828403/150‑1 (MQ=255)
                                                                                            cgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCat                                                          <  1:16898/150‑2 (MQ=255)
                                                                                            cgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAg                                                          <  1:1038853/150‑1 (MQ=255)
                                                                                            cgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACAGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCCCCACCAGCGACGGTGCAAAACGCCACACATCCAGTCCGGCATTCAg                                                          >  2:84883/1‑150 (MQ=255)
                                                                                                 ccGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCa                                                     <  1:666009/150‑1 (MQ=255)
                                                                                                  cGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCAt                                                    >  1:1107908/1‑150 (MQ=255)
                                                                                                   gACATCTTTTGCCTGATGCTTCGTATTTACGCACCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGAAAAACGCATCAAATCCGGTCCGGCATTCAGCACCAtt                                                   >  1:1258229/1‑150 (MQ=255)
                                                                                                              ccTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCctc                                        <  1:1074736/150‑1 (MQ=255)
                                                                                                              ccTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCctc                                        >  2:745782/1‑150 (MQ=255)
                                                                                                              ccTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCcta                                        >  2:1260129/1‑149 (MQ=255)
                                                                                                                tGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTg                                      <  2:1094614/150‑1 (MQ=255)
                                                                                                                  aTGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTgcg                                    <  2:1313506/150‑1 (MQ=255)
                                                                                                                    gCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAACCGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGcc                                  >  1:587829/1‑150 (MQ=255)
                                                                                                                     cTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCGACATTACGCCAGCCTCTGCGccc                                 >  2:1279391/1‑150 (MQ=255)
                                                                                                                      ttCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCg                                <  1:285115/150‑1 (MQ=255)
                                                                                                                           aTTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCCTTACGCCAGCCTCTGCGCCCGCATAc                           >  1:1050034/1‑150 (MQ=255)
                                                                                                                            tttACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACa                          >  1:665516/1‑150 (MQ=255)
                                                                                                                              tACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAgg                        >  1:293062/1‑150 (MQ=255)
                                                                                                                                  ccccAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCCCCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAAt                    >  2:1019554/1‑150 (MQ=255)
                                                                                                                                  ccccAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCCCCATTACGCCAGCCTCTGCGCCCGCATACAGGAAAt                    >  2:1271435/1‑150 (MQ=255)
                                                                                                                                    ccAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCa                  <  1:959771/150‑1 (MQ=255)
                                                                                                                                     cAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCAc                 >  2:406393/1‑150 (MQ=255)
                                                                                                                                     cAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCAACGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCAc                 <  2:1176908/150‑1 (MQ=255)
                                                                                                                                      aaCCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATTAcg                <  2:572606/150‑1 (MQ=255)
                                                                                                                                      aaCCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCAcg                <  2:556369/150‑1 (MQ=255)
                                                                                                                                      aaCCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCAcg                <  2:109160/150‑1 (MQ=255)
                                                                                                                                         caccTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACgcgc             <  1:363869/150‑1 (MQ=255)
                                                                                                                                         acgtttCGCCCACGCGGTGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAGTCACgcgc             >  1:1054731/5‑150 (MQ=255)
                                                                                                                                          cgtttCGCCCACGCGGTGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAATTCACGCGcc            >  2:975182/4‑150 (MQ=255)
                                                                                                                                              tCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCAGCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGAc        <  1:1260115/150‑1 (MQ=255)
                                                                                                                                                 ccACCGCGTGGGCGAAACGTTGTATCCCTTCATCGAAATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCtt     <  2:660882/150‑1 (MQ=255)
                                                                                                                                                 ccACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGTAATCACGCGCCCGAccgt     >  1:53634/1‑148 (MQ=255)
                                                                                                                                                    ccGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGCCCTTTGt  >  2:1123617/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTCAGCGACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGT  >  NC_000913/3488835‑3489132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: