Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,458 A→G 5.0% Y136C (TAT→TGT)  frlR → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,4580AG 5.0% 241.6 / 2.5 79Y136C (TAT→TGT) frlRputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (34/41);  new base G (2/2);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTCAGTGGTTTTACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACC  >  NC_000913/3504309‑3504606
                                                                                                                                                     |                                                                                                                                                    
tGTCAGTGGTTTTACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTa                                                                                                                                                      <  1:394984/150‑1 (MQ=255)
   cAGTGGTTTTACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTAtct                                                                                                                                                   >  1:456312/1‑150 (MQ=255)
   cAGTGGTTTTACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTAtct                                                                                                                                                   >  1:451572/1‑150 (MQ=255)
       ggTTTTACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGAt                                                                                                                                               >  2:1072331/1‑150 (MQ=255)
        gTTTTACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATa                                                                                                                                              <  1:1282616/150‑1 (MQ=255)
         ttttACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATaa                                                                                                                                             <  1:43183/150‑1 (MQ=255)
          tttACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATaaa                                                                                                                                            <  1:966547/150‑1 (MQ=255)
           ttACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAag                                                                                                                                           <  1:705302/150‑1 (MQ=255)
           ttACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAag                                                                                                                                           <  1:718528/150‑1 (MQ=255)
           ttACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAag                                                                                                                                           <  2:504221/150‑1 (MQ=255)
            tACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGACGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGATGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAaga                                                                                                                                          >  1:524135/1‑150 (MQ=255)
              ccGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATTGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGc                                                                                                                                        >  1:358318/1‑150 (MQ=255)
              ccGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGc                                                                                                                                        <  2:1207452/150‑1 (MQ=255)
              ccGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGGCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGc                                                                                                                                        <  2:381227/150‑1 (MQ=255)
                 aTTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCTATCTCTGCCGGGTGATGTGTCTCGATAAAGAGCCGc                                                                                                                                     >  1:678957/1‑150 (MQ=255)
                 aTTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCTATCTCTGCCGGGTGATGTGTCTCGAGAAAGAGCCGc                                                                                                                                     >  1:683462/1‑150 (MQ=255)
                         gTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTAtt                                                                                                                             <  2:584146/150‑1 (MQ=255)
                           ctcACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGa                                                                                                                           <  1:593434/150‑1 (MQ=255)
                           ctcACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGa                                                                                                                           >  2:387258/1‑150 (MQ=255)
                             cacaAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATa                                                                                                                         >  2:780117/1‑150 (MQ=255)
                             cacaAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGGTTATTGATa                                                                                                                         <  1:570873/150‑1 (MQ=255)
                              acaAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAg                                                                                                                        <  1:381381/150‑1 (MQ=255)
                                 aggcaaggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTc                                                                                                                     >  1:861316/1‑150 (MQ=255)
                                    caaggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATg                                                                                                                  <  1:1153013/150‑1 (MQ=255)
                                       ggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATa                                                                                                                                              <  1:470153/119‑1 (MQ=255)
                                       ggcGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATa                                                                                                                                              >  2:470153/1‑119 (MQ=255)
                                         cgacgaAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATcc                                                                                                             >  2:1096604/1‑150 (MQ=255)
                                           acgaAAGAGAAAGTGTTCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAAGAGCGAAGTGTTCCATCTCTGCAGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCg                                                                                                           <  2:801101/150‑1 (MQ=255)
                                             gaAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCt                                                                                                         <  1:995500/150‑1 (MQ=255)
                                             gaAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCt                                                                                                         <  2:732482/150‑1 (MQ=255)
                                             gaAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTACATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCt                                                                                                         >  2:1044887/1‑150 (MQ=255)
                                             gaAAGAGAAAGTGATCGAACAGGAACGGGGCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCt                                                                                                         <  2:1221626/150‑1 (MQ=255)
                                                     aaGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTGTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCAGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGtt                                                                                                 >  2:951830/1‑150 (MQ=255)
                                                         gATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATcc                                                                                             <  2:1301563/150‑1 (MQ=255)
                                                         gATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATcc                                                                                             >  2:584324/1‑150 (MQ=255)
                                                         gATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATcc                                                                                             <  2:1153643/150‑1 (MQ=255)
                                                         gATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGGTATcc                                                                                             >  2:1247917/1‑150 (MQ=255)
                                                         gATCGAACAGGAACGGGGCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCTAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATcc                                                                                             <  2:243778/150‑1 (MQ=255)
                                                            cGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCACGGCCGGGTGATGTATCTCGAGAACGAGCCGCTGTGTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTgc                                                                                          >  2:787488/1‑149 (MQ=255)
                                                              aaCAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACt                                                                                        <  2:1173478/150‑1 (MQ=255)
                                                                   gAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTtgat                                                                                   <  1:492961/150‑1 (MQ=255)
                                                                    aaCGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTtgatg                                                                                  <  1:619824/150‑1 (MQ=255)
                                                                        ggTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGAt                                                                              >  1:1120576/1‑150 (MQ=255)
                                                                             gcgccgcgccGTTTTGCGATCAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATATAGAGCAGCTGTTTATTGATAGTTCATGGATCCCGCAGTCGCGTTATCCTGACTTTGATGAGATTTACg                                                                         <  1:200137/150‑1 (MQ=255)
                                                                             gcgccgcgccGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACg                                                                         <  2:298203/150‑1 (MQ=255)
                                                                             gcgccgcgccGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACg                                                                         <  2:434301/150‑1 (MQ=255)
                                                                             gcgccgcgccGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACg                                                                         <  1:1194759/150‑1 (MQ=255)
                                                                               gccgcgccgTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTc                                                                       <  2:406659/150‑1 (MQ=255)
                                                                                     ccgTTTTGCGAAAAGCTGAACATCCCCGGAACCAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCgaagga                                                                 <  2:967073/150‑1 (MQ=255)
                                                                                     ccgTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCgaagga                                                                 <  1:486525/150‑1 (MQ=255)
                                                                                       gTTTTGCGAAAAGCTGAACATCCCAGGAAAGAGCTAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCAAGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCgaaggaag                                                               <  2:1270324/150‑1 (MQ=255)
                                                                                          ttGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTc                                                            >  2:516117/1‑150 (MQ=255)
                                                                                            gCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCa                                                          >  2:173882/1‑150 (MQ=255)
                                                                                            gCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCa                                                          >  1:583685/1‑150 (MQ=255)
                                                                                                   gCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCa                                                   >  2:36146/1‑150 (MQ=255)
                                                                                                    cTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAg                                                  <  2:1270063/150‑1 (MQ=255)
                                                                                                     tGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATTGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGt                                                 <  1:855371/150‑1 (MQ=255)
                                                                                                     tGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGt                                                 >  1:560985/1‑150 (MQ=255)
                                                                                                     tGAACATCCCCGGAAAAAGCGAAGTCTTAAATCTCTGGAGTATGATGTATCTCGAATAAGCGCCGCTTTTTATTGATAGTTCATGGATCACGCTCTATCGTTATCATGACTTTGATGAGATTTAAGTCGAAGGAAGCTCCAACGATCAGt                                                 >  2:190583/1‑150 (MQ=255)
                                                                                                      gAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGtt                                                >  1:946151/1‑150 (MQ=255)
                                                                                                           tCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTc                                           >  2:572090/1‑150 (MQ=255)
                                                                                                              ccGGACACAGCGAAGTGTTCCAGCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCGGTGTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAgg                                        <  2:963564/150‑1 (MQ=255)
                                                                                                                   aaCAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGTAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGt                                   >  2:1242966/1‑150 (MQ=255)
                                                                                                                      cgCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGtttt                                <  1:560877/149‑1 (MQ=255)
                                                                                                                       gCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTg                               >  1:794150/1‑150 (MQ=255)
                                                                                                                       gCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTg                               >  1:914849/1‑150 (MQ=255)
                                                                                                                             tgtTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACAcgc                         <  1:1191391/150‑1 (MQ=255)
                                                                                                                              gtTCTATCTCTGCCGGGTGATGTGTCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACAcgcg                        <  2:678957/150‑1 (MQ=255)
                                                                                                                              gtTCTATCTCTGCCGGGTGATGTGTCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACAcgcg                        <  2:683462/150‑1 (MQ=255)
                                                                                                                              gtTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTTATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACAcgcg                        <  1:807257/150‑1 (MQ=255)
                                                                                                                              gtTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACAcgcg                        >  1:1139406/1‑150 (MQ=255)
                                                                                                                                   aTCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTgg                   >  1:1102878/1‑150 (MQ=255)
                                                                                                                                            gggTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACa          >  1:502631/1‑150 (MQ=255)
                                                                                                                                                gATGTATCTCGATAAAGAGCCGCTGTTTTTTGATAGTTCATGGATCCCGCTGTCGCGATATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCCAATATCAGTTATTTCAGTAGCGTTATGACTCGCGAGTGGAAAGCGACaaaaa      >  1:231868/1‑150 (MQ=255)
                                                                                                                                                gATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGCAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACaaaaa      >  1:123036/1‑150 (MQ=255)
                                                                                                                                                gATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACaaaaa      <  2:946151/150‑1 (MQ=255)
                                                                                                                                                 aTGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAg     <  1:1047774/150‑1 (MQ=255)
                                                                                                                                                  tGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGa    >  2:461943/1‑150 (MQ=255)
                                                                                                                                                   gTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGAc   <  1:173882/150‑1 (MQ=255)
                                                                                                                                                    tATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGAcc  >  1:1264552/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGTCAGTGGTTTTACCGATTTTGGCGTCTCACAAGGCAAGGCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCAGCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGATAAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAGCTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACC  >  NC_000913/3504309‑3504606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: