Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,530,672 G→C 6.0% intergenic (+20/+43) hslO → / ← yhgE heat shock protein Hsp33/DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,530,6720GC 6.0% 229.5 / 4.9 78intergenic (+20/+43)hslO/yhgEheat shock protein Hsp33/DUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (27/46);  new base C (3/2);  total (30/48)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTT  >  NC_000913/3530523‑3530821
                                                                                                                                                     |                                                                                                                                                     
tAGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCagagag                                                                                                                                                       >  1:1188903/1‑150 (MQ=255)
 aGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAgg                                                                                                                                                      <  1:664390/150‑1 (MQ=255)
  gCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAggg                                                                                                                                                     <  1:104242/150‑1 (MQ=255)
       cTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGGCTCCGGAAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTg                                                                                                                                                <  1:789692/150‑1 (MQ=255)
       cTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAAAGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTg                                                                                                                                                >  2:1181023/1‑150 (MQ=255)
          gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCg                                                                                                                                             <  2:586516/150‑1 (MQ=255)
          gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCg                                                                                                                                             >  2:364149/1‑150 (MQ=255)
          gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTGCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCg                                                                                                                                             <  2:388475/150‑1 (MQ=255)
           cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCgg                                                                                                                                            <  1:1165607/150‑1 (MQ=255)
              aaGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACACCGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGAtt                                                                                                                                         >  2:59180/1‑150 (MQ=255)
                  tGGCGAAATTGCCATGCATTGTGCTTACTTCGGAAACCACTATCTGCTCAATGCGATGGATATTGCGGAAATCCGCAACAACGCGGCTCCGGCAGATCCGCAAGTTCATAAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTAc                                                                                                                                     <  1:950972/150‑1 (MQ=255)
                   ggCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCTCAACAACGCGTCTCAGGCAGATCCGCAAGTTCATTAATGAGTATTTCCGGCAGAGACGCCACTGCCGGATTTTaag                                                                                                                                    >  2:969784/1‑148 (MQ=255)
                     cGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATAAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGAc                                                                                                                                  <  1:806625/150‑1 (MQ=255)
                      gaaagggACATGCATTGTGATTACTGCGGTAACCACTCTCTGTTCAATCCGATGGATATTGCTGAAAGCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACt                                                                                                                                 <  1:185803/144‑1 (MQ=255)
                        aaTTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTaa                                                                                                                               <  2:1130157/150‑1 (MQ=255)
                          ttGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGCAAATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATa                                                                                                                             >  2:814795/1‑150 (MQ=255)
                            gACATGCATTGTGATTACTGCGGGAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATaca                                                                                                                           <  1:681987/150‑1 (MQ=255)
                                 gCATTGTGATTACTGCGGTTACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGa                                                                                                                      <  2:671988/150‑1 (MQ=255)
                                     tgtgATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGTCTAATACACCTGATgcg                                                                                                                  <  1:699146/150‑1 (MQ=255)
                                      gtgATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCTCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATgcgc                                                                                                                 <  2:921493/150‑1 (MQ=255)
                                        taTTAATGCGGTAAGATCTATGTGTTAAATGCGATGTATTTTTCTTAATTCCGCTACAACGCGTCTCCGGCAGATCCTCAAGTTAATTAATGAGTATGTCCGGAAGAGAGGGCTCTGCAGGATTTAACGACTATTACACCTGATGCGCtt                                                                                                               <  2:221488/149‑1 (MQ=255)
                                         aTTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCttt                                                                                                              <  1:846199/150‑1 (MQ=255)
                                           tACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGt                                                                                                            <  1:1188180/150‑1 (MQ=255)
                                            aCTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTc                                                                                                           <  2:1055093/150‑1 (MQ=255)
                                            aCTGCGGTAAACACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGATACAACGCGTCTCCTGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGTGCTTTGTc                                                                                                           <  2:542613/150‑1 (MQ=255)
                                             cTGCGGTAACCACTATCTGTTCATTGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCa                                                                                                          <  2:629301/150‑1 (MQ=255)
                                              tGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCAc                                                                                                         <  1:491321/150‑1 (MQ=255)
                                              tGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCAc                                                                                                         >  2:821258/1‑150 (MQ=255)
                                               gCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACt                                                                                                        <  2:541484/150‑1 (MQ=255)
                                                cGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTc                                                                                                       <  1:1279579/150‑1 (MQ=255)
                                                  gTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTATACGACTAATACACCTGATGCGCTATGTCACTCAt                                                                                                     >  1:1209195/1‑150 (MQ=255)
                                                     aCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCa                                                                                                  <  2:774502/150‑1 (MQ=255)
                                                     aCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGAAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCa                                                                                                  <  1:749091/150‑1 (MQ=255)
                                                     aCCACTATCTGTTCAATGCGATGGATATTCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAg                                                                                                 >  1:1183969/1‑150 (MQ=255)
                                                      ccACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTATTGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAg                                                                                                 >  2:318220/1‑150 (MQ=255)
                                                        aCTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTGATTAATGAGTATCTCCGGCAGAGAGTGCTCTGCCGGATTTTACGAATTATACACCTGATGCGGTTTGTCACTCATTCAGAt                                                                                               >  2:795548/1‑150 (MQ=255)
                                                        aCTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACATGATGCGCTATTTCAAACATTCatat                                                                                               >  2:978085/1‑150 (MQ=255)
                                                            tCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTCATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTcac                                                                                           <  1:621554/150‑1 (MQ=255)
                                                            tCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTcac                                                                                           <  1:896486/150‑1 (MQ=255)
                                                              tGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACAt                                                                                         >  2:343283/1‑150 (MQ=255)
                                                                ttCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTc                                                                                       <  1:1124098/150‑1 (MQ=255)
                                                                 tCAATGCTATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCa                                                                                      <  2:182181/150‑1 (MQ=255)
                                                                    aTGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGcc                                                                                   >  2:1238231/1‑150 (MQ=255)
                                                                     tGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCAGTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCt                                                                                  >  2:1082970/1‑150 (MQ=255)
                                                                       cGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCttt                                                                                >  1:912814/1‑150 (MQ=255)
                                                                        gATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTAAGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTc                                                                               <  2:611867/150‑1 (MQ=255)
                                                                          tGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCAc                                                                             >  2:1154599/1‑150 (MQ=255)
                                                                          tGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCAc                                                                             <  1:1140207/150‑1 (MQ=255)
                                                                               atTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATcc                                                                        <  2:111695/150‑1 (MQ=255)
                                                                                   cTGAAATCCGCAACAACGCGTCTCCGGCAGCTCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGAGGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCGTCCACTa                                                                    >  1:615806/1‑150 (MQ=255)
                                                                                       aaTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAg                                                                >  2:7788/1‑150 (MQ=255)
                                                                                          ccGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACTACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATc                                                             <  1:364149/150‑1 (MQ=255)
                                                                                          ccGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTAAGCCTTTCACCATCCACTAAAAGATc                                                             >  2:1063475/1‑150 (MQ=255)
                                                                                           cGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATcg                                                            <  2:522707/150‑1 (MQ=255)
                                                                                           cGCAACAACGCGTATCCTGCATATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGATCTGCCGGATTTTACGACTAATACACCTGATGCTCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATcg                                                            <  1:1154291/150‑1 (MQ=255)
                                                                                             caacaaCGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTTTGTCCGGGAGAGAGGGCTCTGGCGGATTTTACGACTATTACACCTGCGGCGCTTTGTCGCTCTTTCAGATTGACTGTCGGCCGTTCCCCGTCGACTACATGATagcg                                                          >  2:788981/1‑150 (MQ=255)
                                                                                                caacGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCCGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCa                                                       <  2:708091/150‑1 (MQ=255)
                                                                                                caacGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGACCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCa                                                       <  1:79056/150‑1 (MQ=255)
                                                                                                  ccgcgTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACg                                                     <  2:1172827/149‑1 (MQ=255)
                                                                                                       gctcCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCGCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTa                                                <  2:111787/149‑1 (MQ=255)
                                                                                                         tcCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAgg                                              <  2:487119/150‑1 (MQ=255)
                                                                                                            ggCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCtt                                           <  1:1181023/150‑1 (MQ=255)
                                                                                                              cAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCg                                         <  2:310361/150‑1 (MQ=255)
                                                                                                                 aTCCGCAAGTTGATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCGTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcc                                      <  2:826055/150‑1 (MQ=255)
                                                                                                                 aTCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcc                                      <  1:1251670/150‑1 (MQ=255)
                                                                                                                       aaGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGtt                                >  2:1118452/1‑150 (MQ=255)
                                                                                                                          ttCATTAATGAGTATGTCCGGCAGAGAGGGCGCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTc                             <  2:657819/150‑1 (MQ=255)
                                                                                                                            cATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCCCGCTTTAGGCTTCGTCCCCAGTTTTCCa                           >  2:546578/1‑150 (MQ=255)
                                                                                                                            cATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCa                           <  2:566999/150‑1 (MQ=255)
                                                                                                                                aaTGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCt                       >  1:1037152/1‑150 (MQ=255)
                                                                                                                                aaTGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCt                       <  2:1150335/150‑1 (MQ=255)
                                                                                                                                 aTGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCAATGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGTTCGCGCCACGCTTTAGGCTTCGGCCCCAGTTTTCCATCCtg                      >  1:28202/1‑149 (MQ=255)
                                                                                                                                   gAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCCCTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCg                    >  1:250166/1‑150 (MQ=255)
                                                                                                                                   gAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCGCCAGTTTTCCATCCTTCg                    >  1:190180/1‑150 (MQ=255)
                                                                                                                                       aaatCCGGCAGAGCCCTCTCTGCCGGATTTTACGACTAATACACCAGATGCTCTTTGTCACTCATTAAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCTCGACACGCTTTAGGCTTCGTCCACAGGTTTCCATCCTTCGCAGc                >  1:493430/4‑150 (MQ=255)
                                                                                                                                        tGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCt               >  1:609658/1‑150 (MQ=255)
                                                                                                                                           ccGGCAGAGCCCTCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCCCCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg            >  2:873841/1‑150 (MQ=255)
                                                                                                                                           ccGGCAGAGAGGTCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCATCtgtg            <  1:59180/150‑1 (MQ=255)
                                                                                                                                            cGGCAGAGCGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCCCTCATTCAGATTCACATTCAGGCTTTCACCATCCACTACAAGCTCGCGCCACGCTTTAGGCGTCGTCCCCAGGTGTCCATCCTTCGCAGCtgggg           >  1:349368/1‑147 (MQ=255)
                                                                                                                                            cGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGa           <  2:896939/150‑1 (MQ=255)
                                                                                                                                             ggCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAg          <  1:446670/150‑1 (MQ=255)
                                                                                                                                                  agagGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAtt     >  2:538347/1‑150 (MQ=255)
                                                                                                                                                     gggCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAttttt  <  2:152988/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTT  >  NC_000913/3530523‑3530821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: