Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 318,652 T→A 6.1% intergenic (‑85/+24) rclB ← / ← rclA reactive chlorine species (RCS) stress resistance periplasmic protein/reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913318,6520TA 6.1% 263.1 / 4.8 94intergenic (‑85/+24)rclB/rclAreactive chlorine species (RCS) stress resistance periplasmic protein/reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
Reads supporting (aligned to +/- strand):  ref base T (39/49);  new base A (3/3);  total (42/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

GCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATC  >  NC_000913/318503‑318796
                                                                                                                                                     |                                                                                                                                                
tcATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATAtt                                                                                                                                                  <  2:1034294/149‑1 (MQ=255)
gCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATAtt                                                                                                                                                  >  1:397722/1‑150 (MQ=255)
    tggtggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGtt                                                                                                                                              <  2:565559/150‑1 (MQ=255)
    tggtggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGtt                                                                                                                                              <  2:75438/150‑1 (MQ=255)
    tggtggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGtt                                                                                                                                              <  2:72523/150‑1 (MQ=255)
     ggtggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTc                                                                                                                                             <  1:779391/150‑1 (MQ=255)
     ggtggAAAATGCCATAACGCCACATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGttg                                                                                                                                             >  2:644532/1‑149 (MQ=255)
        ggAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGAGTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGCATAGTTGTTCTgg                                                                                                                                          >  1:526333/1‑150 (MQ=255)
          aaaaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCg                                                                                                                                        >  2:369792/1‑150 (MQ=255)
          aaaaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCg                                                                                                                                        <  1:470926/150‑1 (MQ=255)
          aaaaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAACAAATATTCTGGCg                                                                                                                                        <  2:685348/150‑1 (MQ=255)
           aaaaGCCTTAACGCCAGATAATAGTGTTGCAAATAAATCAGATTTTTTAAACATATATAATACCATCAATGATGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGt                                                                                                                                       <  2:1312887/150‑1 (MQ=255)
            aaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAACAAATATTCTGGCGTc                                                                                                                                      <  1:348370/150‑1 (MQ=255)
            aaTGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGGTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTc                                                                                                                                      <  2:954341/150‑1 (MQ=255)
            aaTGCAATAAATCAAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACTTATAAAATACCATAAATGAAGTTATTAAGTATTTATAGAGTAACATAGAATTAATAATATTATTTTCTCTGAGACGCCAGAAAAAATATTCTGGCGTc                                                                                                                                      <  1:49581/150‑1 (MQ=255)
                 cATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGATAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGAtt                                                                                                                                 >  2:1296181/1‑150 (MQ=255)
                   gaaCGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGATACGCCAGAATATTTGTTCTGGCGACTGAtttt                                                                                                                               >  1:308934/2‑150 (MQ=255)
                    aaCGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACTTATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTg                                                                                                                              <  2:1075572/150‑1 (MQ=255)
                    aaCGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGAttttt                                                                                                                              >  2:244500/1‑149 (MQ=255)
                       gCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATATGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGt                                                                                                                           >  2:1017950/1‑150 (MQ=255)
                          aGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTa                                                                                                                        >  1:899232/1‑150 (MQ=255)
                             taataGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTAttt                                                                                                                     <  1:59670/150‑1 (MQ=255)
                             taataGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCACCAATGAAGTGATTAAGAAGTTATATAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACCCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTAttt                                                                                                                     >  2:950636/1‑150 (MQ=255)
                                taGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGAc                                                                                                                  <  2:472849/150‑1 (MQ=255)
                                  gtgtTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTa                                                                                                                >  1:594743/1‑150 (MQ=255)
                                    gtTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAAt                                                                                                              <  2:1022359/150‑1 (MQ=255)
                                     ttGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATg                                                                                                             <  1:955353/150‑1 (MQ=255)
                                     ttGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATg                                                                                                             <  1:674174/150‑1 (MQ=255)
                                       gCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGATTTAATAAGATTCTTTTCTCTGAGACGCCGGAATATTTGTTCTGGAGTGGGATTTTGTGTTTATTTGACTTATGaa                                                                                                           >  2:1222532/1‑150 (MQ=255)
                                       gCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGaa                                                                                                           >  2:274628/1‑150 (MQ=255)
                                        cAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTTATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGaaa                                                                                                          >  2:752556/1‑150 (MQ=255)
                                                aCAGATTTTTTAAACATATAAAATACCATCAATTAAGTGATTAATAAGTTATAGAGTAACAGATAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCa                                                                                                  <  1:1132535/150‑1 (MQ=255)
                                                aCAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCa                                                                                                  <  1:1031828/150‑1 (MQ=255)
                                                      tttttAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGt                                                                                            <  2:837091/150‑1 (MQ=255)
                                                      tttttAAACATATAAAATACCATCAATGAAGTGATTAAGAACTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGt                                                                                            <  2:362979/150‑1 (MQ=255)
                                                       ttttAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTg                                                                                           <  1:34840/150‑1 (MQ=255)
                                                            aaCATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGTATTAATAAGATTCTTTTCTCTGAGACGCCATAATATTTGTTCTGGCGTCTGATTTTTAGTTTATTTGACTAATGAAAATAGATCATTGTGTGATTCg                                                                                      <  1:601749/150‑1 (MQ=255)
                                                            aaCATAATAAAAACCATCTATGAACTGATAAAGAAGTTATAGAGTAACACTGAAGTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCg                                                                                      <  2:796186/150‑1 (MQ=255)
                                                                 ataAAATACCATCAATGTAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCAt                                                                                 <  2:216921/150‑1 (MQ=255)
                                                                 ataAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTATGGCGTCTGATTTTGAGTTTATATGACTAATGAAAATAGATCATTGAGTGATTCGCTCAt                                                                                 >  2:1143019/1‑150 (MQ=255)
                                                                  taAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATc                                                                                <  1:21944/150‑1 (MQ=255)
                                                                  taAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATc                                                                                <  2:279549/150‑1 (MQ=255)
                                                                       tACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACgg                                                                           <  1:486432/150‑1 (MQ=255)
                                                                       tACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTAATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACgg                                                                           >  2:544994/1‑150 (MQ=255)
                                                                         ccATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATTTTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGAt                                                                         >  2:764316/1‑150 (MQ=255)
                                                                          cATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGATAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATg                                                                        <  2:141418/150‑1 (MQ=255)
                                                                           aTCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGa                                                                       >  1:298831/1‑150 (MQ=255)
                                                                           aTCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGa                                                                       >  2:308117/1‑150 (MQ=255)
                                                                                 gAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTCGCGGCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAat                                                                 >  2:167707/1‑150 (MQ=255)
                                                                                 gAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTAATGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAat                                                                 >  2:907995/1‑150 (MQ=255)
                                                                                  aaGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAata                                                                <  1:1075457/150‑1 (MQ=255)
                                                                                   aGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAatat                                                               >  1:1025585/1‑150 (MQ=255)
                                                                                      gATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTg                                                            <  2:551249/150‑1 (MQ=255)
                                                                                      gATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTg                                                            <  1:274628/150‑1 (MQ=255)
                                                                                            taagTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATcgcg                                                      <  1:734078/149‑1 (MQ=255)
                                                                                              agTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTa                                                    <  2:663961/150‑1 (MQ=255)
                                                                                                   taGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATg                                               >  2:326778/1‑150 (MQ=255)
                                                                                                    agagTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGc                                              >  1:1244383/1‑150 (MQ=255)
                                                                                                    agagTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGc                                              <  1:1077342/150‑1 (MQ=255)
                                                                                                      cgTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCGGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCta                                            <  2:548566/149‑1 (MQ=255)
                                                                                                      agTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCta                                            <  2:727547/150‑1 (MQ=255)
                                                                                                       gTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCtat                                           >  1:123651/1‑150 (MQ=255)
                                                                                                        tAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCtata                                          <  2:536860/150‑1 (MQ=255)
                                                                                                        tAAAAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCtata                                          <  2:541684/150‑1 (MQ=255)
                                                                                                          aCAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAg                                        <  2:397722/150‑1 (MQ=255)
                                                                                                          aCAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAg                                        <  1:227964/150‑1 (MQ=255)
                                                                                                            agagAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGc                                      >  2:820929/1‑150 (MQ=255)
                                                                                                            agagAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGc                                      <  1:1126525/150‑1 (MQ=255)
                                                                                                            agagAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGc                                      <  1:179371/150‑1 (MQ=255)
                                                                                                             gagaATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCa                                     <  2:1092697/150‑1 (MQ=255)
                                                                                                               gaATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGc                                   >  1:1075106/1‑150 (MQ=255)
                                                                                                                   taataaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCgg                               <  1:677842/150‑1 (MQ=255)
                                                                                                                    aataaGATTCTTTTCTCTGAGACGCCAGAATTTTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGc                              >  2:1235057/1‑150 (MQ=255)
                                                                                                                     ataaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGca                             >  2:1051480/1‑150 (MQ=255)
                                                                                                                      taaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACTGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGcat                            >  2:973648/1‑150 (MQ=255)
                                                                                                                             cTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAc                     <  1:1165610/150‑1 (MQ=255)
                                                                                                                             cTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAc                     <  2:1043612/150‑1 (MQ=255)
                                                                                                                             cTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAAAATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAc                     >  1:113889/1‑150 (MQ=255)
                                                                                                                             cTTTTCTCTCAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGGTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAc                     <  2:1033676/150‑1 (MQ=255)
                                                                                                                               tttCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGGTTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCa                   >  1:1079701/1‑150 (MQ=255)
                                                                                                                               tttCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCTTAATATGCTATAAGGCAGCCCGGCATCCATCAcaa                   >  2:1212917/1‑148 (MQ=255)
                                                                                                                               tttCTCTGAGACGCCAGAATATTTGTTCTGCCGTCTGATTTTGAGTTTATTTGCCTAATGAAAATAGATCAATGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCa                   >  2:608881/1‑150 (MQ=255)
                                                                                                                                    ctGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCCACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCCGCCAGCCATCCATCACGATTTTc              >  2:316448/1‑150 (MQ=255)
                                                                                                                                      gagaCGCGAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCAc            <  2:1232841/150‑1 (MQ=255)
                                                                                                                                       agaCGCCAGAACAAATATTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACt           >  1:359485/1‑150 (MQ=255)
                                                                                                                                       agaCGCCAGAACAAATATTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATATTCACt           >  2:757439/1‑150 (MQ=255)
                                                                                                                                        gaCGCCAGAACAAATATTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGCCGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCCTCACCATTTTCACta          >  2:282230/1‑150 (MQ=255)
                                                                                                                                          cGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGTCTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACtata        <  2:451086/150‑1 (MQ=255)
                                                                                                                                          cGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACtata        <  2:848301/150‑1 (MQ=255)
                                                                                                                                           gCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTatat       >  2:646449/1‑150 (MQ=255)
                                                                                                                                           gCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTAAAAGGCAGCCCGGCATCCATCACCATTTTCACTatat       <  2:1284470/150‑1 (MQ=255)
                                                                                                                                            ccAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATAtt      >  2:963613/1‑150 (MQ=255)
                                                                                                                                             cAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATAttt     >  2:90233/1‑149 (MQ=255)
                                                                                                                                               gAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGat   >  2:277804/1‑150 (MQ=255)
                                                                                                                                                aaTATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGatc  <  1:244500/150‑1 (MQ=255)
                                                                                                                                                aaTATTTGTTCTGGCGTCTGATTTTGAGGTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCTTCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGatc  >  1:154053/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GCATTGGTGGAAAATGCCATAACGCCAGATAATAGTGTTGCAAATAAAACAGATTTTTTAAACATATAAAATACCATCAATGAAGTGATTAAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATC  >  NC_000913/318503‑318796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: