Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,663,558 G→T 5.1% intergenic (+31/+332) mdtF → / ← gadW anaerobic multidrug efflux transporter, ArcA‑regulated/transcriptional activator of gadA and gadBC; repressor of gadX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,663,5580GT 5.1% 355.0 / 6.7 117intergenic (+31/+332)mdtF/gadWanaerobic multidrug efflux transporter, ArcA‑regulated/transcriptional activator of gadA and gadBC; repressor of gadX
Reads supporting (aligned to +/- strand):  ref base G (46/65);  new base T (3/3);  total (49/68)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

Only 100 of 117 total aligned reads displayed.

GCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACAT  >  NC_000913/3663411‑3663703
                                                                                                                                                   |                                                                                                                                                 
gcgGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGt                                                                                                                                                 >  2:1128994/1‑150 (MQ=255)
 cgGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGtt                                                                                                                                                >  1:520883/1‑150 (MQ=255)
  ggtaggtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTc                                                                                                                                               >  1:637245/1‑150 (MQ=255)
     aggtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGc                                                                                                                                            <  1:942742/150‑1 (MQ=255)
     aggtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGc                                                                                                                                            <  2:1198905/150‑1 (MQ=255)
      ggtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGcc                                                                                                                                           <  1:904560/150‑1 (MQ=255)
      gggaCCGGCGGGATGGGCGGGATGTTTGCCGCAACAGTGCTGGAAATTTACTTCGTTCCTGGCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGcc                                                                                                                                           <  2:815602/147‑1 (MQ=255)
       gtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCt                                                                                                                                          <  2:175025/150‑1 (MQ=255)
       gtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCt                                                                                                                                          <  1:580955/150‑1 (MQ=255)
       gtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCt                                                                                                                                          <  2:1313101/150‑1 (MQ=255)
           cGGCGTGATGGTCGGGATGTTTGCCGCAACAGTTCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTTAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATTAAGGTTCAGCCTTAct                                                                                                                                      <  1:560513/150‑1 (MQ=255)
            ggCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTActc                                                                                                                                     >  2:903465/1‑150 (MQ=255)
             gCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTActct                                                                                                                                    <  1:249440/150‑1 (MQ=39)
               gTGAAGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTATGGGTGAACATGAAGGTTCAGCCTTACTCttt                                                                                                                                  <  2:713308/150‑1 (MQ=255)
                tGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTc                                                                                                                                 >  1:1293956/1‑150 (MQ=255)
                 gATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTcc                                                                                                                                <  1:903465/150‑1 (MQ=255)
                      gCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTa                                                                                                                           >  2:702769/1‑150 (MQ=255)
                         ggATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAAcc                                                                                                                        >  1:1268851/1‑150 (MQ=255)
                         ggATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAAcc                                                                                                                        >  2:514596/1‑150 (MQ=255)
                         ggATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTATCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTCTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTCCTTAcc                                                                                                                        >  2:472018/1‑150 (MQ=255)
                              tttGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTTAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTca                                                                                                                   >  1:542054/1‑150 (MQ=255)
                                 gCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTcacca                                                                                                                >  2:389327/1‑150 (MQ=255)
                                  ccGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTcaccac                                                                                                               <  2:965931/150‑1 (MQ=255)
                                  ccGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTcaccac                                                                                                               <  2:1085468/150‑1 (MQ=255)
                                    gCATCAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTGACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCCCCACTc                                                                                                             >  1:1158453/1‑150 (MQ=255)
                                     cAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTcc                                                                                                            >  2:724506/1‑149 (MQ=255)
                                          gTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCaacaac                                                                                                       >  1:1041515/1‑150 (MQ=255)
                                            gCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCa                                                                                                     <  2:1067018/150‑1 (MQ=255)
                                                  aaTTTACTTCGTTCCGATCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAAc                                                                                               >  2:130316/1‑150 (MQ=255)
                                                   aTTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAAcc                                                                                              <  1:747082/150‑1 (MQ=255)
                                                       aCTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCa                                                                                          >  1:711773/1‑150 (MQ=255)
                                                        cTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTATATGAGAGTAAGGTTGAACATGCAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCATCTAACCGTCAg                                                                                         >  1:636192/1‑150 (MQ=255)
                                                        cTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAg                                                                                         >  2:476789/1‑150 (MQ=255)
                                                         ttCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAgg                                                                                        <  2:1027469/150‑1 (MQ=255)
                                                         ttCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAgg                                                                                        <  2:778718/150‑1 (MQ=255)
                                                           cGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGaa                                                                                      <  2:1310753/150‑1 (MQ=255)
                                                           cGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGaa                                                                                      >  1:381768/1‑150 (MQ=255)
                                                             ttCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATa                                                                                    >  1:590593/1‑150 (MQ=255)
                                                                cGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCa                                                                                 <  1:153114/150‑1 (MQ=255)
                                                                 ggTCTTTTTCGTTGTAGTGGATGATCTCTTTGCCCGCTTTAATAAAGCGTAACGTGTAAATGAGAGTAAGCTTGACCATGAAGGTTCAGCCTTTCTCTTTCCTGCTAACGATTCACCACTCAACAACCAGCTAACCGTCAGGTATACCAg                                                                                <  2:94437/150‑1 (MQ=255)
                                                                 ggTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACACCCAGCTAACCGTCAGGAATACCAg                                                                                >  1:1315827/1‑150 (MQ=255)
                                                                 ggTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAg                                                                                <  1:80307/150‑1 (MQ=255)
                                                                  gTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAgg                                                                               <  2:308365/150‑1 (MQ=255)
                                                                    cTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCt                                                                             <  1:553631/150‑1 (MQ=255)
                                                                     tttttCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCtt                                                                            <  1:628793/150‑1 (MQ=255)
                                                                       tttCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAg                                                                          <  1:665234/150‑1 (MQ=255)
                                                                        ttCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCAAACCGTCAGGAATACCAGGCTTAGt                                                                         <  2:256884/150‑1 (MQ=255)
                                                                         gcGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATTAGAGTAAGGTTGAACAAGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCCCCACTCACCAACCAGCTACCCGGCAGGAATACCAGGCTAAGTa                                                                        >  2:655929/2‑150 (MQ=255)
                                                                          cGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACAATGCTTAGTac                                                                       >  2:507469/1‑150 (MQ=255)
                                                                              gTAGTTGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTGCAGCCTTACTCGTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAg                                                                   <  1:987900/150‑1 (MQ=255)
                                                                               tAGTGGAACATCTCTTTGCCCGCTTTAGAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGc                                                                  <  2:381387/150‑1 (MQ=255)
                                                                               tAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGc                                                                  <  2:1165634/150‑1 (MQ=255)
                                                                                 gTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCAc                                                                >  2:39840/1‑150 (MQ=255)
                                                                                 gTGGAACATCTATTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCAc                                                                <  1:202129/150‑1 (MQ=255)
                                                                                  tGGAACATCTATTTTCCCGCTTTAAAAAAGCGTAACGTGTAAATGATTGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAAGCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACt                                                               <  2:877791/150‑1 (MQ=255)
                                                                                    gAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGa                                                             <  2:698979/150‑1 (MQ=255)
                                                                                     aaCATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGaa                                                            >  1:1302767/1‑150 (MQ=255)
                                                                                        aTCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGt                                                         <  2:701618/150‑1 (MQ=255)
                                                                                        aTCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGt                                                         <  1:483853/150‑1 (MQ=255)
                                                                                         tctcTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTCCTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGATAGTa                                                        >  1:357407/1‑150 (MQ=255)
                                                                                         tctcTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTa                                                        <  2:637245/150‑1 (MQ=255)
                                                                                         tctcTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTa                                                        >  1:1286074/1‑150 (MQ=255)
                                                                                          ctctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAg                                                       >  1:145962/1‑150 (MQ=255)
                                                                                          ctctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAg                                                       >  1:580510/1‑150 (MQ=255)
                                                                                             tttGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGaaa                                                    <  1:736837/150‑1 (MQ=255)
                                                                                              ttGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAAc                                                   <  2:743357/150‑1 (MQ=255)
                                                                                              ttGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAAc                                                   <  1:1053503/150‑1 (MQ=255)
                                                                                               tGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAAcc                                                  <  2:419331/150‑1 (MQ=255)
                                                                                               tGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAAcc                                                  <  2:1015500/150‑1 (MQ=255)
                                                                                               tGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAAcc                                                  <  1:697374/150‑1 (MQ=255)
                                                                                                gCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCa                                                 >  1:930276/1‑150 (MQ=255)
                                                                                                 cactcTTTAAAAAAGCTTAACGTGTAAATTAGATTAAGGTTTAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCATGCTTAGTACACAGCACTGAAAGTAGAAACCAc                                                <  2:641624/146‑1 (MQ=255)
                                                                                                  ccGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACt                                               <  2:1302767/150‑1 (MQ=255)
                                                                                                   cGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTc                                              >  1:149909/1‑150 (MQ=255)
                                                                                                   cGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTc                                              <  1:564923/150‑1 (MQ=255)
                                                                                                    gCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTcc                                             >  1:1307279/1‑150 (MQ=255)
                                                                                                     cTTTAATAAAGGGAAACGTGTAAATGAGAGTAAGGGTTAACATGAAGGTTCAGCCTTACTCTTTCCTGCTTACCATTCACGTCTCAACAACCAGCTAAGCGTCAGGAAAACCAGGCTTAGTCCTCAGCACTGAAAGTAGAATCCACTCCa                                            <  2:514144/150‑1 (MQ=255)
                                                                                                      tttAAAAAAGCGTTACGTGTAAATGATAGTAAGGTTTAACATGAAGGTTCAGCCTTACAGTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACGGTCTTGAATACCTGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAg                                           <  2:1112777/150‑1 (MQ=255)
                                                                                                       ttAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGc                                          <  1:89215/150‑1 (MQ=255)
                                                                                                                cGTAACGTGTAAATGAGAGTAATGTGGAACATGAAGGTGCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtca                                 <  2:805568/150‑1 (MQ=255)
                                                                                                                cGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtca                                 <  1:781322/150‑1 (MQ=255)
                                                                                                                  tAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtcatc                               <  2:3505/150‑1 (MQ=255)
                                                                                                                   aaCGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtcatca                              <  1:1112462/150‑1 (MQ=255)
                                                                                                                      gtgtAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAAtt                           <  2:282543/150‑1 (MQ=255)
                                                                                                                         tAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTAAACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGa                        <  2:1048196/150‑1 (MQ=255)
                                                                                                                          aaaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTACCCATTCACCACTCAACAACCAGCTAACCGTAAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaa                       >  2:918538/1‑150 (MQ=255)
                                                                                                                           aaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTGACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACAAGGCTTAGTACACAGCACGGAAAGTAGAAACCACTCCAGCACTTCGTCATCAATTTGaaa                      >  1:335347/1‑149 (MQ=255)
                                                                                                                            aTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAAGAACCAGCTAACCGTCAGGAATACCAGGCTTATTAAACAGCACTTAAAGTAGTAACCACTCCAGCCATTCGTCATGAATTTGaaca                     <  1:1038713/150‑1 (MQ=255)
                                                                                                                            aTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaaca                     <  1:789927/150‑1 (MQ=255)
                                                                                                                             tGAGAGTAAGGTTGAGCATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCAATGAAAGTAGAAACCACTCCCGCCATTCGTCTTCATTTTGaacaa                    >  1:198859/1‑150 (MQ=255)
                                                                                                                             tGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGCAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaacaa                    >  2:178665/1‑150 (MQ=255)
                                                                                                                              aagagTAAGGCTGAACCTTCATGTTCAACCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCAATCGTCATCAATTTGaacaac                   >  2:118144/2‑150 (MQ=38)
                                                                                                                               agagTAAGGCTGAACCTTCATGTTCAACCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAacaaca                  >  1:317345/1‑150 (MQ=39)
                                                                                                                                     aGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATAc            <  2:505039/150‑1 (MQ=255)
                                                                                                                                       gTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCGGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAAAAACACAATACTg          >  2:1217473/1‑150 (MQ=255)
                                                                                                                                           aaCATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGAccc      >  1:883995/1‑150 (MQ=255)
                                                                                                                                            aCATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCca     <  2:371602/150‑1 (MQ=255)
                                                                                                                                              aTGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCcaca   <  2:149909/150‑1 (MQ=255)
                                                                                                                                               tGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCCCTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACAt  >  1:981914/1‑150 (MQ=255)
                                                                                                                                               tGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACAt  >  1:1013798/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACAT  >  NC_000913/3663411‑3663703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: