Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,748,108 T→A 9.3% F342Y (TTC→TAC)  lyxK → L‑xylulose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,748,1080TA 9.3% 220.5 / 10.4 91F342Y (TTC→TAC) lyxKL‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base T (37/44);  new base A (4/5);  total (42/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

TGGTGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACG  >  NC_000913/3747959‑3748252
                                                                                                                                                     |                                                                                                                                                
tGGTGAAGATATCGTTCACGAAGCCACCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGGGGGGAGAAATCTCGTTTCATGAGATCATTCAGGCCGTTTCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTATCCTGCCGtt                                                                                                                                                  <  2:924068/150‑1 (MQ=255)
  gTGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTcc                                                                                                                                                <  2:1004659/150‑1 (MQ=255)
   tGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCt                                                                                                                                               >  1:305011/1‑150 (MQ=255)
     aaattATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCATGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGt                                                                                                                                             <  2:559712/147‑1 (MQ=255)
     aaTTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGt                                                                                                                                             >  1:406871/1‑150 (MQ=255)
          aTCGTTCACGTAGCCAGCCCTACCTCTTCCGGCATCCTCGATTGGTTTACCGCACAGTGGGGAGAATTCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgc                                                                                                                                        <  1:1134317/150‑1 (MQ=255)
            cGTTCACGACGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATGTCGTTTGATGAGATCAATCAGGACGTTGCCAGCTGGGCGCGGGCAGGGGGCGGGCGCGGTGTCCTGCCGTTACTGTCCGgcag                                                                                                                                      >  2:60933/1‑150 (MQ=255)
            cGTTCACGAAGCCAGCCCTCCCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGgcag                                                                                                                                      <  1:859876/150‑1 (MQ=255)
            cGTTCACGAAGCCAGCCCTACCTCTTCCGTCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGgcag                                                                                                                                      <  1:864041/150‑1 (MQ=255)
            cGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcag                                                                                                                                      >  2:422798/1‑150 (MQ=255)
            cGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGgcag                                                                                                                                      <  2:756638/150‑1 (MQ=255)
            cGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGgcag                                                                                                                                      <  1:1100146/150‑1 (MQ=255)
             gTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGgcagc                                                                                                                                     <  2:766500/150‑1 (MQ=255)
                 aCGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTACAGGAACGGCAGGaaaa                                                                                                                                 <  2:752889/150‑3 (MQ=255)
                   gAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGcc                                                                                                                               <  1:5674/150‑1 (MQ=255)
                     agccagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCgg                                                                                                                             <  1:298953/150‑1 (MQ=255)
                       ccagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGAc                                                                                                                           >  2:780457/1‑150 (MQ=255)
                       ccagccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGAc                                                                                                                           <  1:1012797/150‑1 (MQ=255)
                          gccCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCg                                                                                                                        >  1:459188/1‑150 (MQ=255)
                            ccTACCTCTTCCGGCAAACTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCCGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCgag                                                                                                                      >  1:11087/1‑150 (MQ=255)
                             cTACCTCTTCCGGCAACCTCGAATGGGTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCgaga                                                                                                                     <  2:960551/150‑1 (MQ=255)
                                 ctctTCCGGCAACCTCGAATGG‑TTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGAcc                                                                                                                <  2:1096977/150‑1 (MQ=255)
                                  tctTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCATTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGAcc                                                                                                                <  1:845075/150‑1 (MQ=255)
                                   ctTCCGGCAACCTCGATTGGTTTACCGCCCAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGACGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCa                                                                                                               >  2:1284706/1‑150 (MQ=255)
                                     tCCGGCAACCTCGATTGGTTTACCGCACAGTGGGGAGAAATCTCGCTTGATGAGATCAATCAGGCCGTTGCCAGCATGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGGAACGACGGACTCGAGATGACCAGt                                                                                                             >  1:275855/1‑150 (MQ=255)
                                     tCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTCCGGCAGCAACGCCGGACTCGAGATGACCAGt                                                                                                             >  2:110294/1‑150 (MQ=255)
                                      ccGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTg                                                                                                            >  2:150908/1‑150 (MQ=255)
                                       cGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTgg                                                                                                           >  1:271132/1‑150 (MQ=255)
                                        ggCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCGACGCCGGACTCGAGATGACCAGTGGt                                                                                                          >  2:1183149/1‑150 (MQ=255)
                                        ggCAACCTCGAATGGTTTACCGAACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGt                                                                                                          >  2:272334/1‑150 (MQ=255)
                                           aaCCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTc                                                                                                       <  2:957548/150‑1 (MQ=255)
                                             ccTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCGTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGCCTCGAGATGACCAGTGGTTTCTa                                                                                                     >  2:248952/1‑150 (MQ=255)
                                              cTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTAc                                                                                                    >  1:417259/1‑150 (MQ=255)
                                               tCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACg                                                                                                   <  1:1025431/150‑1 (MQ=255)
                                                cGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGAGCTCTTTTGCATGCCGTGCCTGTACGGCCGCACCGCCGGCGTCGAGATGACAGGTGGTTAATACgg                                                                                                  >  2:1079279/1‑150 (MQ=255)
                                                 gAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACggg                                                                                                 >  2:923340/1‑150 (MQ=255)
                                                  aaTGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGa                                                                                                <  1:408474/150‑1 (MQ=255)
                                                  aaTGGTTGACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGa                                                                                                <  1:835374/150‑1 (MQ=255)
                                                     ggTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGc                                                                                             <  1:31696/150‑1 (MQ=255)
                                                     ggTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGc                                                                                             <  1:977481/150‑1 (MQ=255)
                                                      gTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCAGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGTCAGCAACGCCGGAATCGAGATGACCAGTGGTTTCAACGGGATGCa                                                                                            >  2:367463/1‑150 (MQ=255)
                                                            cGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCAt                                                                                      >  2:760286/1‑150 (MQ=255)
                                                             gCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCAtt                                                                                     <  2:181763/150‑1 (MQ=255)
                                                              cacaGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTc                                                                                    <  1:736442/150‑1 (MQ=255)
                                                              cacaGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTc                                                                                    >  1:605421/1‑150 (MQ=255)
                                                                 aGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTcaca                                                                                 <  2:994494/150‑1 (MQ=255)
                                                                  gTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCATGCCTAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGACGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCAGTCccac                                                                                >  1:852449/1‑150 (MQ=255)
                                                                      ggAGATATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTCTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGCGATGCAGGCCAGTCACACCcgc                                                                            >  2:719021/1‑150 (MQ=255)
                                                                      ggAGAAATCTCGTTTGATGAGATCAATCAGGCGGTATCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCTTACCTGTACGGCAGCAACGCCGGACTCGAGATGCCGAGTGGTTTCTCCGGGATGCAGGCCATTCACACCcgc                                                                            >  2:520388/1‑150 (MQ=255)
                                                                      ggAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCTTTCCTGTACGGCAGCAACGCCGGACTCGACATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCcgg                                                                            >  2:1211419/1‑149 (MQ=255)
                                                                           aaTCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCAGAAGGATGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACCCCcgcgcgaa                                                                       >  1:1210253/1‑148 (MQ=255)
                                                                            aTCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCAc                                                                      <  2:965459/150‑1 (MQ=255)
                                                                             tctcGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCAGACCCGCGCGCAcc                                                                     <  2:305011/150‑1 (MQ=255)
                                                                             tctcGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCAcc                                                                     <  2:300590/150‑1 (MQ=255)
                                                                             tctcGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCAcc                                                                     <  1:804138/150‑1 (MQ=255)
                                                                                 gTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGtt                                                                 <  2:892328/150‑1 (MQ=255)
                                                                                  gttGCTGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTg                                                                <  2:25696/149‑1 (MQ=255)
                                                                                       ggagaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGc                                                           <  1:1241405/149‑1 (MQ=255)
                                                                                         atatcactctGGCCGTTTCCATCTTGCCGAAGGCTTGGGGGGATCGCTTTTTCCTGCCTTTCCTGTAAGGAAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTTCGGGATGCAGGCCATTCACACCCGCGCGCCCCTGTTGCAGGCCa                                                         <  1:619864/148‑1 (MQ=255)
                                                                                         agaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCa                                                         >  2:514060/1‑150 (MQ=255)
                                                                                          gaTCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAt                                                        <  2:962255/150‑1 (MQ=255)
                                                                                           atcaatcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATc                                                       <  2:361393/150‑1 (MQ=255)
                                                                                             caatgaGGCCGTTGCCTGCTTGGCTAAGGCTGGGGGCTATCTCATTATCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCAGACCCGCGCGCACCTGTTGCAGGCCATCTa                                                     <  1:579988/150‑1 (MQ=255)
                                                                                               atcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGGACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATg                                                   <  2:129704/150‑1 (MQ=255)
                                                                                                  aGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAg                                                <  1:1034275/150‑1 (MQ=255)
                                                                                                   ggCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGATGCAGGCCATCTATGAAgg                                               <  1:272334/150‑1 (MQ=255)
                                                                                                   ggCCGGTGCCAGCTGGCCGAAGGCGGGGGGCGAGCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAgg                                               <  1:58220/150‑1 (MQ=255)
                                                                                                      cGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGggt                                            >  2:19632/1‑150 (MQ=255)
                                                                                                       gTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCAGACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGggtg                                           <  1:923340/150‑1 (MQ=255)
                                                                                                        ttGCCAGCTTGCCGAAGGCTGGGGGCGAGCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGggtgg                                          <  2:490629/150‑1 (MQ=255)
                                                                                                          gCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGgtggtg                                        >  1:1220050/1‑150 (MQ=255)
                                                                                                           ccAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGgtgt                                       >  1:980004/1‑150 (MQ=255)
                                                                                                            cAGCTGGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGtt                                      <  1:444885/150‑1 (MQ=255)
                                                                                                               cTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAg                                   <  2:616817/150‑1 (MQ=255)
                                                                                                               cTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAg                                   >  1:105041/1‑150 (MQ=255)
                                                                                                                 tGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGCCCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCAGCTATGAAGGGGTGGTGTTCAGcc                                 >  1:975064/1‑150 (MQ=255)
                                                                                                                 tGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGcc                                 >  1:1046861/1‑150 (MQ=255)
                                                                                                                   ccgccggCGGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCCGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCat                               <  2:217043/145‑1 (MQ=255)
                                                                                                                   ccGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCCCACCCGCGCGCACCTGTTGCAGGCCCTCTATGAAGGGGTGGTGTTCAGCCat                               >  1:418902/1‑150 (MQ=255)
                                                                                                                      aaGTCTGGGGGCGATCTCTTTTTCCTGCCTTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCTCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATg                            <  1:482493/150‑1 (MQ=255)
                                                                                                                      aaGGCTGGGTGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCTGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATg                            <  2:681004/150‑1 (MQ=255)
                                                                                                                       aGGCTGGGGGCGATCTCTTTTTCCTGCCGTACCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCTCACCAGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGACATATGa                           >  1:965040/1‑150 (MQ=255)
                                                                                                                        ggCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAc                          <  1:482837/150‑1 (MQ=255)
                                                                                                                          cTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAccc                        >  1:832090/1‑150 (MQ=255)
                                                                                                                           tGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGGGGTGTTCAGCCATATGACCCa                       <  2:406085/150‑1 (MQ=255)
                                                                                                                             ggggCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCAcc                     <  2:1096748/150‑1 (MQ=255)
                                                                                                                                 cGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCaa                 >  2:1290816/1‑150 (MQ=255)
                                                                                                                                       ctTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAAt           >  2:1203046/1‑150 (MQ=255)
                                                                                                                                             gcTGCCGTACAGGAACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCCCCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGCCCCACCTCAACCGAATGCGCGa     >  2:896203/2‑150 (MQ=255)
                                                                                                                                             gcTGCCGTACAGGAACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGa     >  2:739484/2‑150 (MQ=255)
                                                                                                                                               tGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAAc   >  2:1211884/1‑150 (MQ=255)
                                                                                                                                                gCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACg  >  1:1167042/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TGGTGAATTTATCGTTCACGAAGCCAGCCCTACCTCTTCCGGCAACCTCGAATGGTTTACCGCACAGTGGGGAGAAATCTCGTTTGATGAGATCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACG  >  NC_000913/3747959‑3748252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑