Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,757,942 C→A 7.4% intergenic (‑115/+75) yiaY ← / ← selB L‑threonine dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,757,9420CA 7.4% 210.8 / 7.4 81intergenic (‑115/+75)yiaY/selBL‑threonine dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor
Reads supporting (aligned to +/- strand):  ref base C (39/36);  new base A (2/4);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

CACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTC  >  NC_000913/3757795‑3758091
                                                                                                                                                   |                                                                                                                                                      
cacaGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCAt                                                                                                                                                      >  1:399517/1‑150 (MQ=255)
  caGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTTAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTa                                                                                                                                                    <  1:977704/150‑1 (MQ=255)
  caGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTa                                                                                                                                                    >  1:398215/1‑150 (MQ=255)
     aaGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAAc                                                                                                                                                <  2:983279/150‑1 (MQ=255)
     aaGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAAc                                                                                                                                                <  1:1250870/150‑1 (MQ=255)
     aaGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAAc                                                                                                                                                <  2:328289/150‑1 (MQ=255)
      aGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCTTTAAACa                                                                                                                                               >  1:549184/1‑150 (MQ=255)
      aGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACa                                                                                                                                               <  1:1190261/150‑1 (MQ=255)
      aGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACa                                                                                                                                               >  2:395168/1‑150 (MQ=255)
        gAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGAACATTGTATTCATTAAACAAt                                                                                                                                             >  1:646939/1‑150 (MQ=255)
          aTAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAAtgt                                                                                                                                           <  2:151911/150‑1 (MQ=255)
            aaaGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCATCTTACTGAGGATTATCATTCGAAAATATGATGATTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTTTAATGAATACAATGTGc                                                                                                                                         <  2:733045/150‑1 (MQ=255)
            aaaGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGc                                                                                                                                         <  2:151604/150‑1 (MQ=255)
               gAACGTTTAATCTGCCATAACACACTACTGAAATTTTAAAAAAGGTCTGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTTTAATGAATACAATGTGCttt                                                                                                                                      <  1:680717/150‑1 (MQ=255)
                  cGTTGAAGCTGCCATAACACCCTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATACATTAAACAATGTGCtttttt                                                                                                                                   >  1:1007060/1‑150 (MQ=255)
                  cGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCtttttt                                                                                                                                   <  1:754974/150‑1 (MQ=255)
                   gTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCttttttt                                                                                                                                  >  1:1161905/1‑150 (MQ=255)
                    ttGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTTTCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTa                                                                                                                                 <  1:967365/150‑1 (MQ=255)
                     tgtaGCTGCCATTACACACTTCTTTAAGTGTAAAAAAGGTCGGGCAGCTTACTGAGGATTTTCATTCGAAAATAGGATCAGTATTATTGATTATCATTATTAATCTAATAAAAAGCACATTGTTTAATGAATACAATGTGCtttttatta                                                                                                                                <  1:647598/147‑5 (MQ=25)
                     tGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTaa                                                                                                                                <  2:251719/150‑1 (MQ=255)
                        aGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAAtt                                                                                                                             >  2:901427/1‑150 (MQ=255)
                        aGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAAtt                                                                                                                             >  1:679013/1‑150 (MQ=255)
                         gCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTc                                                                                                                            >  2:510515/1‑150 (MQ=255)
                         gCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCAAATTTTATTCATTAAAAAATGTGCTTTTTTTAAAtta                                                                                                                            >  2:726017/1‑149 (MQ=255)
                           tGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCAt                                                                                                                          >  2:710601/1‑150 (MQ=255)
                            gCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATa                                                                                                                         <  2:795213/150‑1 (MQ=255)
                            gCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATa                                                                                                                         <  1:734579/150‑1 (MQ=255)
                            gCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGAACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATa                                                                                                                         >  2:515431/1‑150 (MQ=255)
                              cATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATaat                                                                                                                       >  1:452007/1‑149 (MQ=255)
                                       cTCCTTAAAGTTTATAAAAGTGCGTGCAGCTTATAGAGGATTTTCATTCTAAAATATGATCAGTATTATTGATTATCAATATTAATCTAATAAAAATCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCTTATGAAtttaaaa                                                                                                              <  1:274328/150‑5 (MQ=255)
                                                  gtAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAatttattt                                                                                                   >  1:771325/1‑150 (MQ=255)
                                                   tAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTAtttt                                                                                                  <  2:1083485/150‑1 (MQ=255)
                                                     aaaaaGGGCGTGCAGCTTAATGATGTTTTTCATTAGAAAATATGATCAGTATTATTGATTATAAAAATTAATCTAATAAAAAGCACATTGTATTCATTATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTaa                                                                                                <  1:641941/150‑1 (MQ=255)
                                                          gggcgggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACtt                                                                                           >  1:8834/1‑150 (MQ=255)
                                                              gggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACttaatt                                                                                       <  1:264602/150‑1 (MQ=255)
                                                                   tattACTGATGATTTTCATTCGAAAATATGATGAGTATTATTTATTATCAAAATTAATCTAATAAAAAGCAAATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCa                                                                                  <  1:923304/148‑1 (MQ=255)
                                                                   gCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCa                                                                                  <  2:369512/150‑1 (MQ=255)
                                                                     ttACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCAtt                                                                                >  2:1063109/1‑150 (MQ=255)
                                                                      tACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCAttt                                                                               >  2:34257/1‑150 (MQ=255)
                                                                            ggATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTAt                                                                         <  1:1184207/150‑1 (MQ=255)
                                                                              aTTTTCATTAGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTAttt                                                                       <  1:879688/150‑1 (MQ=255)
                                                                                  tCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTcc                                                                   <  2:753052/150‑1 (MQ=255)
                                                                                   cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                                  >  2:146093/1‑150 (MQ=255)
                                                                                   cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                                  >  1:1072733/1‑150 (MQ=255)
                                                                                          aaaTATGATCATTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAaataa                                                           <  1:109748/150‑1 (MQ=255)
                                                                                          aaaTATGATCAGAATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTCTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCATGAaataa                                                           >  2:830507/1‑150 (MQ=255)
                                                                                             tatGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAaataataa                                                        <  1:1087909/150‑1 (MQ=255)
                                                                                               tGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATg                                                      <  2:466741/150‑1 (MQ=255)
                                                                                               tGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATg                                                      >  1:393085/1‑150 (MQ=255)
                                                                                                taTCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGc                                                     <  1:390620/149‑1 (MQ=255)
                                                                                                 aTCAGTATTATTGATTATCAATATTAATCTAATAAAAAGCACAATGTATTCATTTAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTATAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCg                                                    <  2:846099/150‑1 (MQ=255)
                                                                                                   cAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCGTTATTTTTCCGGAAATAATAATGCGTc                                                  >  2:489433/1‑150 (MQ=255)
                                                                                                    aGTATTATTGATTATAAAAATTAATCTAATAAAAAGGACATTGTATTCATTAATCAATTTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCTGAAATAATTTTGCGTcg                                                 <  1:519532/150‑1 (MQ=255)
                                                                                                        ttattGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGTAAATAATAATGCGTCGCGCa                                             >  1:558719/1‑150 (MQ=255)
                                                                                                        ttattGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCa                                             <  2:831134/150‑1 (MQ=255)
                                                                                                           ttGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATa                                          <  2:1077941/150‑1 (MQ=255)
                                                                                                            tGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATaa                                         >  2:653929/1‑150 (MQ=255)
                                                                                                                  tCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATc                                   <  2:716077/150‑1 (MQ=255)
                                                                                                                   cAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCa                                  <  2:372435/150‑1 (MQ=255)
                                                                                                                    aaaaTTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCAt                                 >  1:1214539/1‑150 (MQ=255)
                                                                                                                     aaaTTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGAGTCGCGCAATAAATGATCAtt                                >  2:1268614/1‑150 (MQ=255)
                                                                                                                         tAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCa                            <  1:1013261/150‑1 (MQ=255)
                                                                                                                          aaTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCac                           >  2:1300355/1‑150 (MQ=255)
                                                                                                                           aTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacg                          >  1:700269/1‑150 (MQ=255)
                                                                                                                           aTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacg                          <  1:1063109/150‑1 (MQ=255)
                                                                                                                             cTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGAGTCGCGCAATAAATGATCATTTcaaagaa                        >  1:212528/1‑144 (MQ=255)
                                                                                                                               aataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacga                      >  2:599520/1‑150 (MQ=255)
                                                                                                                               aataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacga                      >  2:605027/1‑150 (MQ=255)
                                                                                                                                   aaaaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGAcgcg                  <  2:8834/150‑1 (MQ=255)
                                                                                                                                     aaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCTCAATAAATGATCATTTCCACGACGACGCTTa                >  2:185844/1‑150 (MQ=255)
                                                                                                                                     aaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTa                >  2:1062619/1‑150 (MQ=255)
                                                                                                                                     aaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTa                <  2:1020877/150‑1 (MQ=255)
                                                                                                                                      aGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCAACGACGACGCGTaa               >  1:507201/1‑150 (MQ=255)
                                                                                                                                      aGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa               >  2:521762/1‑150 (MQ=255)
                                                                                                                                       gCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaaa              >  2:178689/1‑150 (MQ=255)
                                                                                                                                       gCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaaa              >  1:484577/1‑150 (MQ=255)
                                                                                                                                       ccaaaTTGTATTAATTAAACAATGTGCTTTTTTTAAATTCATAATAATTTTGTATTAATTTATTTTAAAACATAATTAATCATTTCCTTATTTTTCCGGTAATAATAATGCGTCTCGCAATAAATGATCATTTCCACGACGACGCGTaaa              <  1:832277/146‑1 (MQ=255)
                                                                                                                                          caTTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCTTAAAGcc           >  2:159804/1‑150 (MQ=255)
                                                                                                                                          caTTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTACTTATTTTTCCTGAAATAATAATTCTTCTCGAAATAAATTATCATTTCCAAGACGACTAGTAAAgaa           >  1:113939/1‑148 (MQ=255)
                                                                                                                                              gTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATTCGTCGCGCAATAAATTATCATTTCCACGACGACGCGTAAAGCCAATg       >  2:215113/1‑150 (MQ=255)
                                                                                                                                                 ttCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCgg    <  1:941623/150‑1 (MQ=255)
                                                                                                                                                   cATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCTTAAAGCCAATGCGGta  >  1:464757/1‑149 (MQ=255)
                                                                                                                                                   cATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGta  >  1:1019603/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
CACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTC  >  NC_000913/3757795‑3758091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: