Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,798,235 A→T 6.6% intergenic (+28/+4) waaL → / ← waaU O‑antigen ligase/lipopolysaccharide core biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,798,2350AT 6.6% 286.1 / 7.4 100intergenic (+28/+4)waaL/waaUO‑antigen ligase/lipopolysaccharide core biosynthesis
Reads supporting (aligned to +/- strand):  ref base A (42/51);  new base T (5/2);  total (47/53)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 102 total aligned reads displayed.

TGGCATTGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGATATTCCGG  >  NC_000913/3798087‑3798382
                                                                                                                                                    |                                                                                                                                                   
tGGCATTGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTaaa                                                                                                                                                    >  2:1229649/1‑150 (MQ=255)
tGGCATTGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGATATAGTCCTCTTACTCGTCATTAAAAAACGTAACAATACAATTAATTAAGAATAAACAATTTTAAGAAGAGAGTTaaa                                                                                                                                                    >  1:576987/1‑150 (MQ=255)
     ttGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAATCAAGTTTAAGAAGTGAGTTAAAACTCa                                                                                                                                               >  1:488697/1‑150 (MQ=255)
      tgtgGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCAATCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCAc                                                                                                                                              <  1:631233/150‑1 (MQ=255)
        tgGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTTTAACTCActt                                                                                                                                            <  2:539428/150‑1 (MQ=255)
        gggggATGGTACTCTGTGAGGTTATAATATGGGCCGGAAGCATTCAAATTAGCATTAAATCCGATATCGCCCTCTAACAGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCActtc                                                                                                                                           <  2:745034/149‑1 (MQ=255)
            gATTGGACGCAGGGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTACTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTa                                                                                                                                        <  2:221116/150‑1 (MQ=255)
             aTTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATAAAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTtattat                                                                                                                                       >  1:133057/1‑150 (MQ=255)
               tGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCt                                                                                                                                     <  1:682587/150‑1 (MQ=255)
                ggACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCta                                                                                                                                    >  2:647947/1‑150 (MQ=255)
                 gACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCtat                                                                                                                                   >  1:651953/1‑150 (MQ=255)
                   cTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTTAGTTAAAACTCACTTCTTATCtataa                                                                                                                                 >  1:467683/1‑149 (MQ=255)
                     cAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACaa                                                                                                                               <  1:294787/150‑1 (MQ=255)
                     cAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCAATTCTTATATATaaaa                                                                                                                               >  1:169794/1‑150 (MQ=255)
                      aGTGATGTGATCATATGGGCACGCAGCATACCAATTATCATTATATCCTCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAAATAATTAACAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAAc                                                                                                                              <  1:59475/150‑1 (MQ=255)
                      aGTGATGTGATCATAAGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAAc                                                                                                                              <  1:120926/150‑1 (MQ=255)
                       gtgatgtgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACt                                                                                                                             <  2:347341/150‑1 (MQ=255)
                            ttgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCTCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAAtc                                                                                                                        <  1:237004/149‑1 (MQ=255)
                            gtgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAAtc                                                                                                                        <  1:876361/150‑1 (MQ=255)
                            gtgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAAtc                                                                                                                        <  2:1016612/150‑1 (MQ=255)
                            gtgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAAtc                                                                                                                        <  2:391196/150‑1 (MQ=255)
                             tgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTTAACAAATTAAtct                                                                                                                       >  1:1038679/1‑150 (MQ=255)
                             tgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAAtct                                                                                                                       <  2:1019770/150‑1 (MQ=255)
                             tgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAAtct                                                                                                                       >  2:1146970/1‑150 (MQ=255)
                             tgatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAAtct                                                                                                                       <  2:597385/150‑1 (MQ=255)
                              gatCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTAAATCGTCATTAATAAACGTAACAATACAATTAAATAAGAATAATCAAGATTAAGAAGTGAGTTAAAACTCACATCTTATCTATACAACTTAAtctc                                                                                                                      >  1:820186/1‑150 (MQ=255)
                                tCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCtt                                                                                                                    >  2:518279/1‑150 (MQ=255)
                                   tatGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTAc                                                                                                                 >  2:846433/1‑150 (MQ=255)
                                    atGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACt                                                                                                                >  1:1158933/1‑150 (MQ=255)
                                     tGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACtt                                                                                                               >  2:814001/1‑149 (MQ=255)
                                         cACGCCGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAACCTCACTTCTTATCTATACAACTTAATCTCTTTACTGAAtt                                                                                                           >  1:1133561/1‑150 (MQ=255)
                                          aCGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTa                                                                                                          >  1:130487/1‑150 (MQ=255)
                                          aCGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTa                                                                                                          <  1:1189580/150‑1 (MQ=255)
                                          aCGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTa                                                                                                          <  1:177949/150‑1 (MQ=255)
                                          aCGCAGCATTACAATTATAATTATATCCTCTATAGTCCTCTTACTAGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTa                                                                                                          <  1:48885/150‑1 (MQ=255)
                                           cGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAg                                                                                                         >  1:458961/1‑150 (MQ=255)
                                            gcagcaTTCCAATTATAATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGt                                                                                                        <  1:701460/150‑1 (MQ=255)
                                                 aTTACAATTATCATTATATAAGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAATAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAg                                                                                                   <  1:43658/150‑1 (MQ=255)
                                                    ccAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGttt                                                                                                >  1:1164079/1‑150 (MQ=255)
                                                    ccAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGttt                                                                                                >  1:428552/1‑150 (MQ=255)
                                                       aTTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAg                                                                                             <  2:1208949/150‑1 (MQ=255)
                                                        ttATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGt                                                                                            <  1:446397/150‑1 (MQ=255)
                                                              ttATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGtt                                                                                                       <  1:1175073/133‑1 (MQ=255)
                                                              ttATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGtt                                                                                                       >  2:1175073/1‑133 (MQ=255)
                                                              ttATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGat                                                                                      <  1:1040488/150‑1 (MQ=255)
                                                                    ccGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCCATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCttt                                                                                <  2:1194193/150‑1 (MQ=255)
                                                                      gCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTa                                                                              >  1:1029034/1‑150 (MQ=255)
                                                                        tataGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTAcc                                                                            <  2:464435/150‑1 (MQ=255)
                                                                               ctctTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTAACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATg                                                                     >  1:1248266/1‑150 (MQ=255)
                                                                                tctTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGt                                                                    <  1:1255656/150‑1 (MQ=255)
                                                                                tctTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGt                                                                    <  1:1146970/150‑1 (MQ=255)
                                                                                tctTACTAGTCATTAATAATCTTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGt                                                                    <  1:58086/150‑1 (MQ=255)
                                                                                 ctTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGtt                                                                   >  1:1297290/1‑150 (MQ=255)
                                                                                   tACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTgg                                                                 <  2:905822/150‑1 (MQ=255)
                                                                                    aCTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACTAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTggg                                                                <  1:801771/150‑1 (MQ=255)
                                                                                      tCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGa                                                              <  2:633792/150‑1 (MQ=255)
                                                                                        gTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTGAGTTTCGATATGTTTTTGGGTTGATGTTggggttt                                                            >  1:1222452/1‑147 (MQ=255)
                                                                                        gTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATa                                                            >  1:114016/1‑150 (MQ=255)
                                                                                          cATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACt                                                          <  1:1210313/150‑1 (MQ=255)
                                                                                          cATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACt                                                          >  2:1311816/1‑150 (MQ=255)
                                                                                            ttAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTTGGGATACTAt                                                        >  2:1283809/1‑150 (MQ=255)
                                                                                               ataatCGTAACAATACAATTAAATAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGGTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTg                                                     <  2:450865/150‑1 (MQ=255)
                                                                                                taatCTTAACAATACAATTAATTAAGAATAAACTAGTTTAAGTATTGAGTTAAAACTAACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATTTTGGGGATACTATTTGt                                                    <  1:595262/150‑1 (MQ=255)
                                                                                                  atCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTTTATGTTGGGGATACTATTTGtat                                                  >  2:248684/1‑150 (MQ=255)
                                                                                                     gTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATTTTGGGGATAATATTTGTATAGa                                               >  1:110666/1‑150 (MQ=255)
                                                                                                         aaatacaatTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTa                                           <  1:649121/149‑1 (MQ=255)
                                                                                                           atacaatTAATTAAGAATAATCAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTAAACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGTGATACTATTTTTATAGATTTatt                                         >  1:957622/1‑149 (MQ=255)
                                                                                                            tacaatTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTatga                                        <  1:1309204/150‑1 (MQ=255)
                                                                                                                attaattaAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATgatgat                                    >  2:194850/1‑150 (MQ=255)
                                                                                                                      taaGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCgggg                              <  1:110596/150‑1 (MQ=255)
                                                                                                                        aGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTAGCGATATCTTTTAACGTATATGTTGGGGATACTATTTGTAGTGATTTATTATGATTCGGGGAc                            >  2:1183428/1‑150 (MQ=255)
                                                                                                                         taaTAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGAcc                           <  1:814001/149‑1 (MQ=255)
                                                                                                                           aTAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCaa                         >  1:443176/1‑150 (MQ=255)
                                                                                                                              aaCAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATTTCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAAtt                      >  1:1040211/1‑150 (MQ=255)
                                                                                                                              aaCAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAAtt                      <  2:315308/150‑1 (MQ=255)
                                                                                                                              aaCAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGATAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTAGTGGAACAAAtt                      >  1:1154506/1‑150 (MQ=255)
                                                                                                                                cAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTaa                    <  1:866444/150‑1 (MQ=255)
                                                                                                                                cAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGATTCAGTATCGTTATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTaa                    <  2:477269/150‑1 (MQ=255)
                                                                                                                                 aaGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTaaa                   <  1:950355/150‑1 (MQ=255)
                                                                                                                                  aGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAAt                  >  2:810771/1‑150 (MQ=255)
                                                                                                                                   ttttAAGAAGTGAGTTATAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTAATTATCGATATCTTTTACCGTATATGTTGGGGATTCTATTTGTATAGATTTATGATGATTAGGGGACCAAATTAAATg                 <  2:576987/149‑1 (MQ=255)
                                                                                                                                   ttttAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCAGGGACCAAATTAAATg                 <  2:1297290/149‑1 (MQ=255)
                                                                                                                                   agatAAGAAGTGAGTTTTAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATg                 >  2:212525/4‑150 (MQ=255)
                                                                                                                                    tttAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGc                <  2:1241826/150‑1 (MQ=255)
                                                                                                                                    tttAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGc                <  2:406535/150‑1 (MQ=255)
                                                                                                                                      tAAGAAGTGAGTTTTAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGa              >  1:418786/1‑150 (MQ=255)
                                                                                                                                       aagaagTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGATTTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTAGTTGTATAGATTTATGATGATTCTGGGACCAAATTAAATGCGAg             >  1:998667/1‑150 (MQ=255)
                                                                                                                                        agaagTTAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAgg            <  2:344387/150‑1 (MQ=255)
                                                                                                                                        agaagTGAGTTTTAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAgg            >  1:462775/1‑150 (MQ=255)
                                                                                                                                        agaagTGAGTTAAATCTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAgg            <  2:1134512/150‑1 (MQ=255)
                                                                                                                                         gaagTGAGTTTTAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAggg           >  1:874735/1‑150 (MQ=255)
                                                                                                                                         gaagTGAGTTTTAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAggg           >  1:910854/1‑150 (MQ=255)
                                                                                                                                         gaagTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAggg           >  2:1152063/1‑150 (MQ=255)
                                                                                                                                         gaagTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAggg           >  2:644350/1‑150 (MQ=255)
                                                                                                                                           agTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATTGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGat         >  2:197680/1‑150 (MQ=255)
                                                                                                                                           agTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGat         >  1:989488/1‑150 (MQ=255)
                                                                                                                                             tGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGatat       <  2:467683/150‑1 (MQ=255)
                                                                                                                                               aGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGATATTc     <  1:744247/150‑1 (MQ=255)
                                                                                                                                                  tAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGATATTCCgg  <  2:1123218/150‑1 (MQ=255)
                                                                                                                                                  tAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCATATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGATATTCCgg  <  2:797424/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGCATTGTGGGGATTGGACTCAGTGATGTGATCATATGGGCACGCAGCATTCCAATTATCATTATATCCGCTATAGTCCTCTTACTCGTCATTAATAATCGTAACAATACAATTAATTAAGAATAAACAAGTTTAAGAAGTGAGTTAAAACTCACTTCTTATCTATACAACTTAATCTCTTTACTGAATTAGTTAAAGTTTCAGTATCGATATCTTTTACCGTATATGTTGGGGATACTATTTGTATAGATTTATGATGATTCGGGGACCAAATTAAATGCGAGGGATATTCCGG  >  NC_000913/3798087‑3798382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: