Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,809,359 T→G 5.8% F274V (TTC→GTC)  waaA → 3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,809,3590TG 5.8% 290.2 / 3.6 105F274V (TTC→GTC) waaA3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)
Reads supporting (aligned to +/- strand):  ref base T (44/53);  new base G (2/5);  total (47/58)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTT  >  NC_000913/3809213‑3809507
                                                                                                                                                  |                                                                                                                                                    
gccgccAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTcc                                                                                                                                                   <  2:1040078/150‑1 (MQ=255)
gccgccAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTcc                                                                                                                                                   >  2:716002/1‑150 (MQ=255)
gccgccAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTcc                                                                                                                                                   <  2:541063/150‑1 (MQ=255)
 ccgccAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTccc                                                                                                                                                  <  1:182460/150‑1 (MQ=255)
    ccAGTGGGCAACACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGa                                                                                                                                               <  2:177958/150‑1 (MQ=255)
      aGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATg                                                                                                                                             >  2:171707/1‑150 (MQ=255)
       gTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGc                                                                                                                                            <  2:654605/150‑1 (MQ=255)
       gTGGGCACCACACCGCCCGGTAGGGATGGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGGAAGCGTTCCGGATga                                                                                                                                            <  2:642465/150‑2 (MQ=255)
         gggCACCACTCCGCCCGGTATGGATAGCCACCAGCACTAACGAAGTCGAAGAGAGTTTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCACGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGGAAGCGTTCCGGATgacg                                                                                                                                          <  2:25178/150‑4 (MQ=255)
          ggCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGAt                                                                                                                                         >  2:696171/1‑150 (MQ=255)
            caccacACCGCCCGGTATGGAATGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGGAAGCGTTCCGGATgacgggg                                                                                                                                       <  1:1058368/150‑7 (MQ=255)
                actccGCCCGGTATGGATTGCAACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCt                                                                                                                                   <  1:1293303/147‑1 (MQ=255)
                acacCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGCTCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCt                                                                                                                                   >  1:105742/1‑150 (MQ=255)
                acacCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCt                                                                                                                                   >  1:1036510/1‑150 (MQ=255)
                 cacCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCTTTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCtt                                                                                                                                  >  1:761881/1‑150 (MQ=255)
                 cacCGCCCGGTATGGATTGACACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCtt                                                                                                                                  >  1:1068111/1‑150 (MQ=255)
                   ccGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACTGCAATTCCCGAATTTATTGCTCATCCTGGTACACCGTCATCCGGACCGCTTCCCGGATGCGATTAACCTTGt                                                                                                                                >  2:1018099/1‑150 (MQ=255)
                   ccGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGt                                                                                                                                >  1:516579/1‑150 (MQ=255)
                   ccGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCAGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGt                                                                                                                                >  2:1243853/1‑150 (MQ=255)
                    cGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGTCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTc                                                                                                                               >  1:721562/1‑150 (MQ=255)
                      cccGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGCACGCTTCCCGGATGCGATTAACCTTGTccc                                                                                                                             >  2:895777/1‑149 (MQ=255)
                      cccGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCg                                                                                                                             <  2:537364/150‑1 (MQ=255)
                         ggTATGGATTGCCACCAGCACTCACGAAGGAGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCa                                                                                                                          <  1:147601/150‑1 (MQ=255)
                          gTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAg                                                                                                                         <  1:903552/150‑1 (MQ=255)
                           tATGGATTGCCCCCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCACGCATTGTTACAGCAATTCCCGAAGTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGTTGCGATTAACCTTGTCCGCCAgg                                                                                                                        >  1:872429/1‑150 (MQ=255)
                           tATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                                                        <  1:1199650/150‑1 (MQ=255)
                            aTGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGc                                                                                                                       <  1:649494/150‑1 (MQ=255)
                              ggATTGCCACCAGTACTCACGAAGGCGAAGCGAGTGTGGTGATCGCCGCACATCAGGCATTGTTAAAGCAATTCCCGAATTTATTGCTCATCCTTGTACCCCGTCCTCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTg                                                                                                                     <  2:359343/150‑1 (MQ=255)
                               gATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCTCATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTgg                                                                                                                    >  1:1068317/1‑150 (MQ=255)
                               gATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTgg                                                                                                                    >  2:975825/1‑150 (MQ=255)
                                aTTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGATTTTATTGCTCATCCTGTTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGa                                                                                                                   >  2:208410/1‑150 (MQ=255)
                                aTTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGa                                                                                                                   >  2:403064/1‑150 (MQ=255)
                                      cccaGCACTCACGCATGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGc                                                                                                             <  1:1228057/149‑1 (MQ=255)
                                      accaGCACTCACGAAGGCGAAGAGAGTGGGGTGAGCGCCGCACATCAGGCATTGTTACAGCAATTGCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGc                                                                                                             <  2:163763/150‑1 (MQ=255)
                                       ccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCt                                                                                                            >  2:137315/1‑150 (MQ=255)
                                         aGCACTCACGAAGGCGAAGAGAGTGTGCTGATCGCCGCACATCAGGCATTGTATCAGCAATTCACGTATGTATAGCTAATCCTGCTACCCCGTCATCCTGAACGCATCCCGGATTCGATTACCCTTCTACGCCCGGCTGGCCtcaccaat                                                                                                          >  1:191069/1‑143 (MQ=255)
                                          gCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCtata                                                                                                         >  2:621063/1‑150 (MQ=255)
                                            aCTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATc                                                                                                       <  1:137315/150‑1 (MQ=255)
                                            aCTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATc                                                                                                       <  2:971649/150‑1 (MQ=255)
                                            aCTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATc                                                                                                       >  1:739040/1‑150 (MQ=255)
                                             cTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTACCCTTGTCCGCCATGCTGGACTAAGGTATATct                                                                                                      >  2:207928/1‑149 (MQ=255)
                                             cTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATca                                                                                                      <  2:960906/150‑1 (MQ=255)
                                               cACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTACCCTTGTCCGCCAGGCTGGACTAAGCTATATcacc                                                                                                    >  2:1003645/1‑149 (MQ=255)
                                                aCGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCCTCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTTGACTAAGCTATATCacac                                                                                                   <  1:657540/150‑1 (MQ=255)
                                                aCGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCacac                                                                                                   <  2:946942/150‑1 (MQ=255)
                                                aCGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCacac                                                                                                   <  2:411813/150‑1 (MQ=255)
                                                aCGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCacac                                                                                                   <  2:397063/150‑1 (MQ=255)
                                                    aGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGctc                                                                                               <  1:84318/150‑1 (MQ=255)
                                                      gCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCGTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCtt                                                                                             >  1:950370/1‑150 (MQ=255)
                                                       cGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTc                                                                                            >  2:1000952/1‑150 (MQ=255)
                                                         aaGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAg                                                                                          <  1:577511/150‑1 (MQ=255)
                                                           gagagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAggg                                                                                        <  1:1311839/150‑1 (MQ=255)
                                                           gagagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAggg                                                                                        <  1:372965/150‑1 (MQ=255)
                                                             gagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGa                                                                                      <  2:923344/150‑1 (MQ=255)
                                                              agTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGGACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGaa                                                                                     <  2:668747/150‑1 (MQ=255)
                                                                tgtgGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCCTCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGt                                                                                   <  1:1227631/150‑1 (MQ=255)
                                                                tgtgGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGCAGt                                                                                   <  1:153015/150‑1 (MQ=255)
                                                                 gtggtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTc                                                                                  >  1:346239/1‑150 (MQ=255)
                                                                    gtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGAACTAAGCTATATCACACGCTCTTCAGGGGAAGTcccc                                                                               <  2:343794/150‑1 (MQ=255)
                                                                      gATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTc                                                                             >  2:929365/1‑150 (MQ=255)
                                                                       aTCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGGACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTcc                                                                            <  2:1277828/150‑1 (MQ=255)
                                                                         cggcgcACATCCGGCATTGTGACAGCAATTCACGAATTTATTGCTCATCCTGGTACCCCGTCATCAGGAACGCTGCCCGGATGCGAATAACCTTGTCCGCCACTCTGCACTAAGCTAAATCACACCATCTTCAGGGGAAGTACCcaccac                                                                          >  1:863268/4‑150 (MQ=255)
                                                                           ccgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTccacca                                                                        >  1:874577/1‑150 (MQ=255)
                                                                              cacaTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCa                                                                     <  2:928740/150‑1 (MQ=255)
                                                                              cacaTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCa                                                                     <  1:577138/150‑1 (MQ=255)
                                                                               acaTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCAc                                                                    <  1:578242/150‑1 (MQ=255)
                                                                                        aTTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTtgt                                                           >  2:402220/1‑150 (MQ=255)
                                                                                           gTTACAGCAATTCCCGACTTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCGTCCCGGATGCGATTAACCGTGTCCGCCAGGCTGGACTAAGCTATCTCACACGCTCTTCAGGGGAAGTCCCCGCCACCAGCACGCAggtggtggt                                                        <  1:82448/150‑1 (MQ=255)
                                                                                             tACAGCAATTCCCGAATTTATTGCTCATCCTGGTTCCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGGTGTGGTTg                                                      >  1:973903/1‑150 (MQ=255)
                                                                                               cAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGc                                                    <  1:886826/150‑1 (MQ=255)
                                                                                               cAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGc                                                    >  1:297219/1‑150 (MQ=255)
                                                                                                aGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCg                                                   <  2:792113/150‑1 (MQ=255)
                                                                                                 gCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTATCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGa                                                  >  1:260173/1‑150 (MQ=255)
                                                                                                  cAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGAt                                                 <  2:1150342/150‑1 (MQ=255)
                                                                                                    aTTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATAc                                               >  2:256603/1‑150 (MQ=255)
                                                                                                        ccGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATg                                           >  1:401894/1‑150 (MQ=255)
                                                                                                         cGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCGGGACTAAGCTATAGCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATgg                                          <  2:372253/150‑1 (MQ=255)
                                                                                                         cGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATAAACCTTGTCCGCCAGGCTGGACTAAGCGATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCACCACGCAGGTTGTGGTTGGCGATACGAAgg                                          >  1:1132577/1‑150 (MQ=255)
                                                                                                            aTTTTTTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGGCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCg                                       <  2:253833/150‑1 (MQ=255)
                                                                                                              ttATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAg                                     <  2:1032574/150‑1 (MQ=255)
                                                                                                               tATTGCTCATCCTGGTACCCCGTCATCCGTAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAgc                                    <  1:452266/150‑2 (MQ=255)
                                                                                                               tATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGt                                    <  1:407581/150‑1 (MQ=255)
                                                                                                                 ttGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTATCCTTGTCCGCCAGGCTGGACTAAGCTATATCTCACGCTCTTCAGGGGAAGTCCCCTCCACCATCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTg                                  <  1:666711/150‑1 (MQ=255)
                                                                                                                   gCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCCCACGATGTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGAt                                >  1:977125/1‑150 (MQ=255)
                                                                                                                    cTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATg                               >  1:720032/1‑150 (MQ=255)
                                                                                                                          cTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTg                         <  1:171707/150‑1 (MQ=255)
                                                                                                                            ggTCCCACGTCATCCTGAACGCTTCCCGGATGCGATTAACCTTGGCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGATGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTa                       <  1:638630/150‑1 (MQ=255)
                                                                                                                               ttaatcgcATCCGGGAAGCGTTCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATgg                    >  2:320674/8‑150 (MQ=255)
                                                                                                                                 cccGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCa                  >  1:1206901/1‑150 (MQ=255)
                                                                                                                                 cccGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCa                  >  2:1156192/1‑150 (MQ=255)
                                                                                                                                  ccGTCATCCGGAACGCTTCCCGGATGCGAGTAACCTTGTCCGCCCGGCGGGACTAAGCTATATCAGACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCAt                 <  2:503143/150‑1 (MQ=255)
                                                                                                                                    gTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTg               <  2:1036510/150‑1 (MQ=255)
                                                                                                                                    gTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCAGTg               >  2:81203/1‑150 (MQ=255)
                                                                                                                                     tcatcaGTAACGCTTCCCGGATGCGCTTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACCCGCTCATCCGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACTATGGGCGAGTTGATGTAACTGTATGGCATTGc              <  2:822341/150‑1 (MQ=255)
                                                                                                                                     tCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGc              >  1:919164/1‑150 (MQ=255)
                                                                                                                                       aTCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCg            <  2:874577/150‑1 (MQ=255)
                                                                                                                                         ccGGAACGCGTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATCGCATTGCCGAt          <  1:919216/150‑1 (MQ=255)
                                                                                                                                             aaCGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCg      <  1:716002/150‑1 (MQ=255)
                                                                                                                                                gCTTCCCGGATGCGATTAACCATGTCCGCCAGGCTGGACTAAGCGATATCACACGCTCATCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCAGTGCCGATCTCGCCt   <  1:75397/150‑1 (MQ=255)
                                                                                                                                                 cTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCACCACCAGCAAGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCtt  >  1:1018104/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTT  >  NC_000913/3809213‑3809507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: