Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,811,212 C→T 6.9% intergenic (‑60/+38) mutM ← / ← rpmG formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase/50S ribosomal subunit protein L33

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,811,2120CT 6.9% 273.8 / 8.1 101intergenic (‑60/+38)mutM/rpmGformamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase/50S ribosomal subunit protein L33
Reads supporting (aligned to +/- strand):  ref base C (40/54);  new base T (5/2);  total (45/56)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01

Only 100 of 109 total aligned reads displayed.

GTTGCGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAG  >  NC_000913/3811063‑3811361
                                                                                                                                                     |                                                                                                                                                     
 ttGCGCACCACTGCATGAAGAATGGGTGCACCAAAGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCATATAACAAAAAAcccc                                                                                                                                                      <  2:974602/150‑1 (MQ=255)
   gcgcACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCccgc                                                                                                                                                    >  2:1306112/1‑150 (MQ=255)
    cgcACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCccgcc                                                                                                                                                   <  2:921058/150‑1 (MQ=255)
      caccacTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCgg                                                                                                                                                 <  1:232787/150‑1 (MQ=255)
        ccacTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAg                                                                                                                                               >  1:488357/1‑150 (MQ=255)
           cTGCATGAAGAATGGTTGCACCAACGAGATGCGGGTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCCGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGa                                                                                                                                            >  1:709090/1‑150 (MQ=255)
            tGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAg                                                                                                                                           <  1:1243178/150‑1 (MQ=255)
             gCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGTGCGAgg                                                                                                                                          >  2:647097/1‑150 (MQ=255)
             gCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTGTCAACTTCGGGTAATTCAGGCATTGCATCTCCATGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAgg                                                                                                                                          >  2:836605/1‑150 (MQ=255)
              cATGAAGATTGGTTGAACCAACGAGATGCGGGTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAAGAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAATACCCCGCCGGAGCGAGGt                                                                                                                                         <  2:1307961/150‑1 (MQ=255)
              cATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAAGTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGt                                                                                                                                         >  1:201074/1‑150 (MQ=255)
               aTGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGATTGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtt                                                                                                                                        <  1:1006097/150‑1 (MQ=255)
               aTGAAGAATGGTTGCACCAAAGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCAGCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtt                                                                                                                                        <  1:1000044/150‑1 (MQ=255)
                 gaagaaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtttt                                                                                                                                      <  1:12612/150‑1 (MQ=255)
                  aagaaTGGTTGGACCAACTAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGTGTAATTCATGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGGCGGAGCGTGGttttt                                                                                                                                     <  2:981612/150‑1 (MQ=255)
                   agaaTGGTAGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCTCGCTCCGGCGGGGtttttt                                                                                                                                    <  1:623972/150‑1 (MQ=255)
                    gaaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGAGGCTGGTTTCAACTTCGGGTAATTCAGGCAAAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTg                                                                                                                                   <  2:572439/150‑1 (MQ=255)
                    gaaTGGTTGCACCAACGAGATGCGGTGCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTg                                                                                                                                   <  2:903982/150‑1 (MQ=255)
                      aTGGTTGCACCAACGAGCTGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGGAATTCAGGCATCGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCTCGCTCCGGCGGGGTTTTTTGtt                                                                                                                                 <  1:1155546/150‑1 (MQ=255)
                      aTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGtt                                                                                                                                 >  2:1249765/1‑150 (MQ=255)
                       tGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTa                                                                                                                                <  2:302611/150‑1 (MQ=255)
                        ggTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGACTGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTAc                                                                                                                               <  2:1162622/150‑1 (MQ=255)
                         gTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACa                                                                                                                              >  2:792758/1‑150 (MQ=255)
                             cACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCaa                                                                                                                          >  2:426287/1‑150 (MQ=255)
                              aCCAACGAGATGCGGTTCAATGCCGCGGCGGCTGGTTTCAACTTAGGTTAATTCAGGCATAGCAGCTCCAGGAATGAACAGATGCAGGCAATATGTGCGCAAGCAGATACCTAAAAACCCCGCCGGAGCGAGGGTTTTTGTTACATCaaa                                                                                                                         <  1:368488/150‑1 (MQ=255)
                               ccAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAg                                                                                                                        >  2:1183743/1‑150 (MQ=255)
                                 aaCGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCg                                                                                                                      <  1:1068748/150‑1 (MQ=255)
                                   cGAGATGCGGTTCTATGCCGCGGCGGCTGGTTACAACATCGGGTAATTAAGGCATAGCATATCCAGGAATGAACAGAAGCAGTCAATATGGGGGCAATCAGATAACAAAAAACCCCCCCGCAGCGAGTTTTTTTGTTAAATCAAAGCgac                                                                                                                    >  2:314993/1‑149 (MQ=255)
                                      taTGCTGTTCTATGCCGCGGAGGCTGGTTTCAACTTAGTGTAATTCAGGCATAGCATATCCAGGAATGAACAGATTCAGTCAATATGGGGGCAAGCAGATAACAAAACACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAat                                                                                                                 <  2:641515/149‑1 (MQ=255)
                                          ctgTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAattatt                                                                                                             <  1:81973/148‑1 (MQ=255)
                                            gTTCTATGCCGCGGCGGCTGGTTTCAACTACGGGTAATTCAGGCCTAGCATCTCCAGGAATGTACAGTTTCAGTCAATATTGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTg                                                                                                           <  2:209752/150‑1 (MQ=255)
                                                tATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGAttt                                                                                                       >  1:257981/1‑150 (MQ=255)
                                                   gCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCg                                                                                                    <  1:679906/150‑1 (MQ=255)
                                                   gCCGCGGCGGCTGGTTTCAACCTCGGGTAATTCAGGCATAGGATCTCCACGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCg                                                                                                    <  2:1101033/150‑1 (MQ=255)
                                                    ccGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGc                                                                                                   <  2:1020539/150‑1 (MQ=255)
                                                     cgcgGCGGCTGGTATCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATTAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGct                                                                                                  <  2:911183/150‑1 (MQ=255)
                                                         gcggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGCTGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGAGAGGTGTATTGTTACATCAAAGCGAGAATTATTTGATTTTCGcttctt                                                                                              >  1:834314/1‑150 (MQ=255)
                                                          cggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCttt                                                                                             >  1:1316009/1‑150 (MQ=255)
                                                           ggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTg                                                                                            >  1:525991/1‑150 (MQ=255)
                                                           ggcTGCTTTCAACTTCGGGTAAATCAGGCATAGCATCTCCAGGAATGAACAGAAGCAGTCAATATGGGGGCAAGCAGATAACAAAATACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTg                                                                                            <  1:121701/150‑1 (MQ=255)
                                                            gcTGGTTTCAACCTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGt                                                                                           >  1:182913/1‑150 (MQ=255)
                                                             cTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTa                                                                                          <  1:1054985/150‑1 (MQ=255)
                                                             cTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTa                                                                                          >  1:699495/1‑150 (MQ=255)
                                                                gTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGAt                                                                                       >  2:88044/1‑150 (MQ=255)
                                                                gTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGAt                                                                                       >  1:97420/1‑150 (MQ=255)
                                                                   tCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACACCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTAATTGATTTTCGCTTCTTTGTAGATCAc                                                                                    >  1:1113282/1‑150 (MQ=255)
                                                                    cAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACg                                                                                   <  1:1210335/150‑1 (MQ=255)
                                                                       cTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGc                                                                                <  1:1201288/150‑1 (MQ=255)
                                                                             gTAATTGATGCATATCATCTCCATGAATGAACAGATGCAGTCAATATGGGGGCAATCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGa                                                                          <  2:1082306/150‑1 (MQ=255)
                                                                              tAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaa                                                                         <  2:237530/150‑1 (MQ=255)
                                                                                  tCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaacaac                                                                     <  1:966648/150‑1 (MQ=255)
                                                                                  tCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaacaac                                                                     >  1:993181/1‑150 (MQ=255)
                                                                                   cAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACt                                                                    <  2:712236/150‑1 (MQ=255)
                                                                                     ggCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTgg                                                                  <  1:750790/150‑1 (MQ=255)
                                                                                      gCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCACATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGa                                                                 >  1:925310/1‑150 (MQ=255)
                                                                                         tAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATATCAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGAGTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCg                                                              >  1:622093/1‑150 (MQ=255)
                                                                                         aaGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCg                                                              <  2:75214/149‑1 (MQ=255)
                                                                                              tctcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAttt                                                         >  1:900051/1‑150 (MQ=255)
                                                                                               ctcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGGTTCTTTGTATATCACGTGCTGTCGAACAACTGGATCGAAtttt                                                        >  2:537738/1‑150 (MQ=255)
                                                                                               ctcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttt                                                        >  1:1110790/1‑150 (MQ=255)
                                                                                                tcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTTTTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAttttt                                                       >  2:69291/1‑150 (MQ=255)
                                                                                                tcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAttttt                                                       >  2:93171/1‑150 (MQ=255)
                                                                                                 ccAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTGGATCACGTGCTGGCGAACAACTGGATCGAAtttttt                                                      >  1:365994/1‑150 (MQ=255)
                                                                                                 ccAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttttt                                                      <  1:44523/150‑1 (MQ=255)
                                                                                                 ccAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttttt                                                      >  1:1111139/1‑150 (MQ=255)
                                                                                                   aGGAATGACCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCa                                                    <  1:619584/150‑1 (MQ=255)
                                                                                                    ggAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAg                                                   >  2:478207/1‑150 (MQ=255)
                                                                                                    ggAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATAACGTGCTGGCGAACAACTGGATCGAATTTTTTCAg                                                   >  1:500034/1‑150 (MQ=255)
                                                                                                        tGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTcc                                               <  1:1312187/150‑1 (MQ=255)
                                                                                                        tGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTcc                                               <  2:656229/150‑1 (MQ=255)
                                                                                                          aaCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCTAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAg                                             >  1:154159/1‑150 (MQ=255)
                                                                                                          aaCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAg                                             >  2:213799/1‑150 (MQ=255)
                                                                                                           aCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGt                                            <  1:1183743/150‑1 (MQ=255)
                                                                                                            cAGATCAAGTCAATATGGGGGCAAGCAGATAACAAAAAACACCGCAGCAGCGAGGTATTTTGTTACATCAAACCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGtt                                           <  2:37885/150‑1 (MQ=255)
                                                                                                              gATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGtttt                                         >  1:1035588/1‑150 (MQ=255)
                                                                                                              gATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGtttt                                         <  2:760433/150‑1 (MQ=255)
                                                                                                                    gTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGc                                   <  2:1272934/150‑1 (MQ=255)
                                                                                                                    gTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGc                                   >  2:466666/1‑150 (MQ=255)
                                                                                                                             ggggCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGt                          >  2:54088/1‑150 (MQ=255)
                                                                                                                              gggCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAGTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGtt                         >  1:1236964/1‑150 (MQ=255)
                                                                                                                               gtcAAGCAGATAACAAAATACCCCGCCGGAGGGAGGTTTTTTGTTACATCATAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGATCAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGttt                        <  1:1296831/148‑1 (MQ=255)
                                                                                                                                gCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTg                       <  2:1316282/150‑1 (MQ=255)
                                                                                                                                   aGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGttc                    >  2:1300846/1‑150 (MQ=255)
                                                                                                                                          aaCAAAAAACCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAg             >  2:720637/1‑150 (MQ=255)
                                                                                                                                           aCAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGt            <  1:1249765/150‑1 (MQ=255)
                                                                                                                                            cAAAAAACCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTg           >  1:1160048/1‑150 (MQ=255)
                                                                                                                                            cAAAAAACCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTg           >  1:306979/1‑150 (MQ=255)
                                                                                                                                            cAAAAAACCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTg           >  2:1021449/1‑150 (MQ=255)
                                                                                                                                             aaaaaaCCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg          >  1:1189310/1‑150 (MQ=255)
                                                                                                                                             aaaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg          <  2:1296606/150‑1 (MQ=255)
                                                                                                                                             aaaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg          <  2:427362/150‑1 (MQ=255)
                                                                                                                                              aaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGt         <  2:1278166/150‑1 (MQ=255)
                                                                                                                                               aaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGta        <  2:182913/150‑1 (MQ=255)
                                                                                                                                               aaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGta        <  2:552543/150‑1 (MQ=255)
                                                                                                                                               aaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGta        <  2:547735/150‑1 (MQ=255)
                                                                                                                                                  aCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAg     <  1:1306112/150‑1 (MQ=255)
                                                                                                                                                   ccccGCCGGAGCGAGGTTTTTTGTTTCATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACTGTTGTTCTTCGTAGTGGTATAGa    >  2:227874/1‑150 (MQ=255)
                                                                                                                                                   ccccGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGa    <  1:1026818/150‑1 (MQ=255)
                                                                                                                                                   ccccGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGATCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGa    <  1:213799/150‑1 (MQ=255)
                                                                                                                                                     ccgcagGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAg  <  1:109627/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTGCGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAG  >  NC_000913/3811063‑3811361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: