Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,811,216 C→T 6.8% intergenic (‑64/+34) mutM ← / ← rpmG formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase/50S ribosomal subunit protein L33

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,811,2160CT 6.8% 284.9 / 8.0 104intergenic (‑64/+34)mutM/rpmGformamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase/50S ribosomal subunit protein L33
Reads supporting (aligned to +/- strand):  ref base C (41/55);  new base T (5/2);  total (46/58)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

Only 100 of 109 total aligned reads displayed.

CGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGA  >  NC_000913/3811067‑3811364
                                                                                                                                                     |                                                                                                                                                    
cgcACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCccgcc                                                                                                                                                      <  2:921058/150‑1 (MQ=255)
  caccacTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCgg                                                                                                                                                    >  1:775027/1‑150 (MQ=255)
  caccacTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCgg                                                                                                                                                    <  1:232787/150‑1 (MQ=255)
    ccacTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAg                                                                                                                                                  >  1:488357/1‑150 (MQ=255)
       cTGCATGAAGAATGGTTGCACCAACGAGATGCGGGTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCCGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGa                                                                                                                                               >  1:709090/1‑150 (MQ=255)
        tGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAg                                                                                                                                              <  1:1243178/150‑1 (MQ=255)
         gCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGTGCGAgg                                                                                                                                             >  2:647097/1‑150 (MQ=255)
         gCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTGTCAACTTCGGGTAATTCAGGCATTGCATCTCCATGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAgg                                                                                                                                             >  2:836605/1‑150 (MQ=255)
          cATGAAGATTGGTTGAACCAACGAGATGCGGGTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAAGAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAATACCCCGCCGGAGCGAGGt                                                                                                                                            <  2:1307961/150‑1 (MQ=255)
          cATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAAGTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGt                                                                                                                                            >  1:201074/1‑150 (MQ=255)
           aTGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGATTGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtt                                                                                                                                           <  1:1006097/150‑1 (MQ=255)
           aTGAAGAATGGTTGCACCAAAGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCAGCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtt                                                                                                                                           <  1:1000044/150‑1 (MQ=255)
             gaagaaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtttt                                                                                                                                         <  1:12612/150‑1 (MQ=255)
              aagaaTGGTTGGACCAACTAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGTGTAATTCATGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGGCGGAGCGTGGttttt                                                                                                                                        <  2:981612/150‑1 (MQ=255)
               agaaTGGTAGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCTCGCTCCGGCGGGGtttttt                                                                                                                                       <  1:623972/150‑1 (MQ=255)
                gaaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGAGGCTGGTTTCAACTTCGGGTAATTCAGGCAAAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTg                                                                                                                                      <  2:572439/150‑1 (MQ=255)
                gaaTGGTTGCACCAACGAGATGCGGTGCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTg                                                                                                                                      <  2:903982/150‑1 (MQ=255)
                  aTGGTTGCACCAACGAGCTGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGGAATTCAGGCATCGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCTCGCTCCGGCGGGGTTTTTTGtt                                                                                                                                    <  1:1155546/150‑1 (MQ=255)
                  aTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGtt                                                                                                                                    >  2:1249765/1‑150 (MQ=255)
                   tGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTa                                                                                                                                   <  2:302611/150‑1 (MQ=255)
                    ggTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGACTGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTAc                                                                                                                                  <  2:1162622/150‑1 (MQ=255)
                     gTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACa                                                                                                                                 >  2:792758/1‑150 (MQ=255)
                      ttGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACAt                                                                                                                                <  2:1152856/150‑1 (MQ=255)
                         cACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCaa                                                                                                                             >  2:426287/1‑150 (MQ=255)
                          aCCAACGAGATGCGGTTCAATGCCGCGGCGGCTGGTTTCAACTTAGGTTAATTCAGGCATAGCAGCTCCAGGAATGAACAGATGCAGGCAATATGTGCGCAAGCAGATACCTAAAAACCCCGCCGGAGCGAGGGTTTTTGTTACATCaaa                                                                                                                            <  1:368488/150‑1 (MQ=255)
                           ccAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAg                                                                                                                           >  2:1183743/1‑150 (MQ=255)
                             aaCGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCg                                                                                                                         <  1:1068748/150‑1 (MQ=255)
                               cGAGATGCGGTTCTATGCCGCGGCGGCTGGTTACAACATCGGGTAATTAAGGCATAGCATATCCAGGAATGAACAGAAGCAGTCAATATGGGGGCAATCAGATAACAAAAAACCCCCCCGCAGCGAGTTTTTTTGTTAAATCAAAGCgac                                                                                                                       >  2:314993/1‑149 (MQ=255)
                                  taTGCTGTTCTATGCCGCGGAGGCTGGTTTCAACTTAGTGTAATTCAGGCATAGCATATCCAGGAATGAACAGATTCAGTCAATATGGGGGCAAGCAGATAACAAAACACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAat                                                                                                                    <  2:641515/149‑1 (MQ=255)
                                   aTGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAatt                                                                                                                   <  2:744933/150‑1 (MQ=255)
                                      ctgTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAattatt                                                                                                                <  1:81973/148‑1 (MQ=255)
                                        gTTCTATGCCGCGGCGGCTGGTTTCAACTACGGGTAATTCAGGCCTAGCATCTCCAGGAATGTACAGTTTCAGTCAATATTGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTg                                                                                                              <  2:209752/150‑1 (MQ=255)
                                            tATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGAttt                                                                                                          >  1:257981/1‑150 (MQ=255)
                                               gCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCg                                                                                                       <  1:679906/150‑1 (MQ=255)
                                                ccGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGc                                                                                                      <  2:1020539/150‑1 (MQ=255)
                                                 cgcgGCGGCTGGTATCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATTAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGct                                                                                                     <  2:911183/150‑1 (MQ=255)
                                                     gcggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGCTGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGAGAGGTGTATTGTTACATCAAAGCGAGAATTATTTGATTTTCGcttctt                                                                                                 >  1:834314/1‑150 (MQ=255)
                                                      cggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCttt                                                                                                >  1:1316009/1‑150 (MQ=255)
                                                       ggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTg                                                                                               >  1:525991/1‑150 (MQ=255)
                                                       ggcTGCTTTCAACTTCGGGTAAATCAGGCATAGCATCTCCAGGAATGAACAGAAGCAGTCAATATGGGGGCAAGCAGATAACAAAATACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTg                                                                                               <  1:121701/150‑1 (MQ=255)
                                                        gcTGGTTTCAACCTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGt                                                                                              >  1:182913/1‑150 (MQ=255)
                                                         cTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTa                                                                                             <  1:1054985/150‑1 (MQ=255)
                                                         cTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTa                                                                                             >  1:699495/1‑150 (MQ=255)
                                                            gTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGAt                                                                                          >  2:88044/1‑150 (MQ=255)
                                                            gTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGAt                                                                                          >  1:97420/1‑150 (MQ=255)
                                                               tCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACACCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTAATTGATTTTCGCTTCTTTGTAGATCAc                                                                                       >  1:1113282/1‑150 (MQ=255)
                                                                cAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACg                                                                                      <  1:1210335/150‑1 (MQ=255)
                                                                   cTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGc                                                                                   <  1:1201288/150‑1 (MQ=255)
                                                                         gTAATTGATGCATATCATCTCCATGAATGAACAGATGCAGTCAATATGGGGGCAATCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGa                                                                             <  2:1082306/150‑1 (MQ=255)
                                                                          tAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaa                                                                            <  2:237530/150‑1 (MQ=255)
                                                                              tCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaacaac                                                                        <  1:966648/150‑1 (MQ=255)
                                                                              tCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaacaac                                                                        >  1:993181/1‑150 (MQ=255)
                                                                               cAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACt                                                                       <  2:712236/150‑1 (MQ=255)
                                                                                  gCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCACATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGa                                                                    >  1:925310/1‑150 (MQ=255)
                                                                                     tAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATATCAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGAGTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCg                                                                 >  1:622093/1‑150 (MQ=255)
                                                                                     aaGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCg                                                                 <  2:75214/149‑1 (MQ=255)
                                                                                          tctcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAttt                                                            >  1:900051/1‑150 (MQ=255)
                                                                                           ctcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGGTTCTTTGTATATCACGTGCTGTCGAACAACTGGATCGAAtttt                                                           >  2:537738/1‑150 (MQ=255)
                                                                                           ctcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttt                                                           >  1:1110790/1‑150 (MQ=255)
                                                                                            tcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTTTTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAttttt                                                          >  2:69291/1‑150 (MQ=255)
                                                                                            tcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAttttt                                                          >  2:93171/1‑150 (MQ=255)
                                                                                             ccAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttttt                                                         >  1:1111139/1‑150 (MQ=255)
                                                                                             ccAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttttt                                                         <  1:44523/150‑1 (MQ=255)
                                                                                               aGGAATGACCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCa                                                       <  1:619584/150‑1 (MQ=255)
                                                                                                ggAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATAACGTGCTGGCGAACAACTGGATCGAATTTTTTCAg                                                      >  1:500034/1‑150 (MQ=255)
                                                                                                    tGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTcc                                                  <  2:656229/150‑1 (MQ=255)
                                                                                                    tGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTcc                                                  <  1:1312187/150‑1 (MQ=255)
                                                                                                      aaCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCTAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAg                                                >  1:154159/1‑150 (MQ=255)
                                                                                                      aaCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAg                                                >  2:213799/1‑150 (MQ=255)
                                                                                                       aCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGt                                               <  1:1183743/150‑1 (MQ=255)
                                                                                                        cAGATCAAGTCAATATGGGGGCAAGCAGATAACAAAAAACACCGCAGCAGCGAGGTATTTTGTTACATCAAACCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGtt                                              <  2:37885/150‑1 (MQ=255)
                                                                                                          gATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGtttt                                            >  1:1035588/1‑150 (MQ=255)
                                                                                                          gATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGtttt                                            <  2:760433/150‑1 (MQ=255)
                                                                                                                gTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGc                                      <  2:1272934/150‑1 (MQ=255)
                                                                                                                gTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGc                                      >  2:466666/1‑150 (MQ=255)
                                                                                                                         ggggCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGt                             >  2:54088/1‑150 (MQ=255)
                                                                                                                          gggCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAGTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGtt                            >  1:1236964/1‑150 (MQ=255)
                                                                                                                            gCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTg                          <  2:1316282/150‑1 (MQ=255)
                                                                                                                               aGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGttc                       >  2:1300846/1‑150 (MQ=255)
                                                                                                                                    aTAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGt                  <  1:1308603/150‑1 (MQ=255)
                                                                                                                                      aaCAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATGTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTat                >  1:1171516/1‑149 (MQ=255)
                                                                                                                                       aCAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGt               <  1:1249765/150‑1 (MQ=255)
                                                                                                                                        cAAAAAACCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTg              >  1:306979/1‑150 (MQ=255)
                                                                                                                                        cAAAAAACCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTg              >  2:1021449/1‑150 (MQ=255)
                                                                                                                                         aaaaaaCCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg             >  1:1189310/1‑150 (MQ=255)
                                                                                                                                         aaaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg             <  2:490789/150‑1 (MQ=255)
                                                                                                                                         aaaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg             <  2:1296606/150‑1 (MQ=255)
                                                                                                                                         aaaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg             <  2:427362/150‑1 (MQ=255)
                                                                                                                                          aaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGt            <  2:1278166/150‑1 (MQ=255)
                                                                                                                                           aaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGta           <  2:547735/150‑1 (MQ=255)
                                                                                                                                           aaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGta           <  2:182913/150‑1 (MQ=255)
                                                                                                                                           aaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGta           <  2:552543/150‑1 (MQ=255)
                                                                                                                                              aCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAg        <  1:1306112/150‑1 (MQ=255)
                                                                                                                                               ccccGCCGGAGCGAGGTTTTTTGTTTCATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACTGTTGTTCTTCGTAGTGGTATAGa       >  2:227874/1‑150 (MQ=255)
                                                                                                                                               ccccGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGa       <  1:1026818/150‑1 (MQ=255)
                                                                                                                                               ccccGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGATCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGa       <  1:213799/150‑1 (MQ=255)
                                                                                                                                                 ccgcagGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAg     <  1:109627/150‑1 (MQ=255)
                                                                                                                                                   gccgGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTg   >  1:1025535/1‑150 (MQ=255)
                                                                                                                                                    ccgGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGa  >  2:431621/1‑150 (MQ=255)
                                                                                                                                                    ccgGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGa  >  1:1209497/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCACCACTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGA  >  NC_000913/3811067‑3811364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: