Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,811,223 A→G 6.4% intergenic (‑71/+27) mutM ← / ← rpmG formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase/50S ribosomal subunit protein L33

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,811,2230AG 6.4% 324.0 / 10.3 110intergenic (‑71/+27)mutM/rpmGformamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase/50S ribosomal subunit protein L33
Reads supporting (aligned to +/- strand):  ref base A (44/58);  new base G (5/2);  total (49/61)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.33e-01

Only 100 of 111 total aligned reads displayed.

CTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTA  >  NC_000913/3811074‑3811370
                                                                                                                                                     |                                                                                                                                                   
cTGCATGAAGAATGGTTGCACCAACGAGATGCGGGTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCCGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGa                                                                                                                                                     >  1:709090/1‑150 (MQ=255)
 tGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAg                                                                                                                                                    <  1:1243178/150‑1 (MQ=255)
  gCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGTGCGAgg                                                                                                                                                   >  2:647097/1‑150 (MQ=255)
  gCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTGTCAACTTCGGGTAATTCAGGCATTGCATCTCCATGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAgg                                                                                                                                                   >  2:836605/1‑150 (MQ=255)
   cATGAAGATTGGTTGAACCAACGAGATGCGGGTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAAGAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAATACCCCGCCGGAGCGAGGt                                                                                                                                                  <  2:1307961/150‑1 (MQ=255)
   cATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAAGTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGt                                                                                                                                                  >  1:201074/1‑150 (MQ=255)
    aTGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGATTGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtt                                                                                                                                                 <  1:1006097/150‑1 (MQ=255)
    aTGAAGAATGGTTGCACCAAAGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCAGCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtt                                                                                                                                                 <  1:1000044/150‑1 (MQ=255)
      gaagaaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGtttt                                                                                                                                               <  1:12612/150‑1 (MQ=255)
       aagaaTGGTTGGACCAACTAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGTGTAATTCATGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGGCGGAGCGTGGttttt                                                                                                                                              <  2:981612/150‑1 (MQ=255)
        agaaTGGTAGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCTCGCTCCGGCGGGGtttttt                                                                                                                                             <  1:623972/150‑1 (MQ=255)
         gaaTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGAGGCTGGTTTCAACTTCGGGTAATTCAGGCAAAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTg                                                                                                                                            <  2:572439/150‑1 (MQ=255)
         gaaTGGTTGCACCAACGAGATGCGGTGCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTg                                                                                                                                            <  2:903982/150‑1 (MQ=255)
           aTGGTTGCACCAACGAGCTGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGGAATTCAGGCATCGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCTCGCTCCGGCGGGGTTTTTTGtt                                                                                                                                          <  1:1155546/150‑1 (MQ=255)
           aTGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGtt                                                                                                                                          >  2:1249765/1‑150 (MQ=255)
            tGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTa                                                                                                                                         <  2:302611/150‑1 (MQ=255)
             ggTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGACTGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTAc                                                                                                                                        <  2:1162622/150‑1 (MQ=255)
              gTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACa                                                                                                                                       >  2:792758/1‑150 (MQ=255)
               ttGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACAt                                                                                                                                      <  2:1152856/150‑1 (MQ=255)
                  cACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCaa                                                                                                                                   >  2:426287/1‑150 (MQ=255)
                   aCCAACGAGATGCGGTTCAATGCCGCGGCGGCTGGTTTCAACTTAGGTTAATTCAGGCATAGCAGCTCCAGGAATGAACAGATGCAGGCAATATGTGCGCAAGCAGATACCTAAAAACCCCGCCGGAGCGAGGGTTTTTGTTACATCaaa                                                                                                                                  <  1:368488/150‑1 (MQ=255)
                    ccAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAg                                                                                                                                 >  2:1183743/1‑150 (MQ=255)
                      aaCGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCg                                                                                                                               <  1:1068748/150‑1 (MQ=255)
                        cGAGATGCGGTTCTATGCCGCGGCGGCTGGTTACAACATCGGGTAATTAAGGCATAGCATATCCAGGAATGAACAGAAGCAGTCAATATGGGGGCAATCAGATAACAAAAAACCCCCCCGCAGCGAGTTTTTTTGTTAAATCAAAGCgac                                                                                                                             >  2:314993/1‑149 (MQ=255)
                           taTGCTGTTCTATGCCGCGGAGGCTGGTTTCAACTTAGTGTAATTCAGGCATAGCATATCCAGGAATGAACAGATTCAGTCAATATGGGGGCAAGCAGATAACAAAACACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAat                                                                                                                          <  2:641515/149‑1 (MQ=255)
                               ctgTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAattatt                                                                                                                      <  1:81973/148‑1 (MQ=255)
                                 gTTCTATGCCGCGGCGGCTGGTTTCAACTACGGGTAATTCAGGCCTAGCATCTCCAGGAATGTACAGTTTCAGTCAATATTGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTg                                                                                                                    <  2:209752/150‑1 (MQ=255)
                                     tATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGAttt                                                                                                                >  1:257981/1‑150 (MQ=255)
                                        gCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCg                                                                                                             <  1:679906/150‑1 (MQ=255)
                                         ccGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGc                                                                                                            <  2:1020539/150‑1 (MQ=255)
                                          cgcgGCGGCTGGTATCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATTAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGct                                                                                                           <  2:911183/150‑1 (MQ=255)
                                              gcggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGCTGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGAGAGGTGTATTGTTACATCAAAGCGAGAATTATTTGATTTTCGcttctt                                                                                                       >  1:834314/1‑150 (MQ=255)
                                               cggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCttt                                                                                                      >  1:1316009/1‑150 (MQ=255)
                                                ggcTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTg                                                                                                     >  1:525991/1‑150 (MQ=255)
                                                ggcTGCTTTCAACTTCGGGTAAATCAGGCATAGCATCTCCAGGAATGAACAGAAGCAGTCAATATGGGGGCAAGCAGATAACAAAATACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTg                                                                                                     <  1:121701/150‑1 (MQ=255)
                                                  cTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTa                                                                                                   <  1:1054985/150‑1 (MQ=255)
                                                  cTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTa                                                                                                   >  1:699495/1‑150 (MQ=255)
                                                     gTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGAt                                                                                                >  2:88044/1‑150 (MQ=255)
                                                     gTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGAt                                                                                                >  1:97420/1‑150 (MQ=255)
                                                         cAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACg                                                                                            <  1:1210335/150‑1 (MQ=255)
                                                            cTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGc                                                                                         <  1:1201288/150‑1 (MQ=255)
                                                                  gTAATTGATGCATATCATCTCCATGAATGAACAGATGCAGTCAATATGGGGGCAATCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGa                                                                                   <  2:1082306/150‑1 (MQ=255)
                                                                   tAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaa                                                                                  <  2:237530/150‑1 (MQ=255)
                                                                       tCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaacaac                                                                              <  1:966648/150‑1 (MQ=255)
                                                                       tCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGaacaac                                                                              >  1:993181/1‑150 (MQ=255)
                                                                        cAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACt                                                                             <  2:712236/150‑1 (MQ=255)
                                                                          ggCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTgg                                                                           <  1:750790/150‑1 (MQ=255)
                                                                           gCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCACATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGa                                                                          >  1:925310/1‑150 (MQ=255)
                                                                              tAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATATCAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGAGTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCg                                                                       >  1:622093/1‑150 (MQ=255)
                                                                              aaGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCg                                                                       <  2:75214/149‑1 (MQ=255)
                                                                                   tctcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGTAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAttt                                                                  <  2:201074/150‑1 (MQ=255)
                                                                                    ctcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGGTTCTTTGTATATCACGTGCTGTCGAACAACTGGATCGAAtttt                                                                 >  2:537738/1‑150 (MQ=255)
                                                                                    ctcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttt                                                                 >  1:1110790/1‑150 (MQ=255)
                                                                                     tcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTTTTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAttttt                                                                >  2:69291/1‑150 (MQ=255)
                                                                                     tcCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAttttt                                                                >  2:93171/1‑150 (MQ=255)
                                                                                      ccAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTGGATCACGTGCTGGCGAACAACTGGATCGAAtttttt                                                               >  1:365994/1‑150 (MQ=255)
                                                                                      ccAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttttt                                                               >  1:1111139/1‑150 (MQ=255)
                                                                                      ccAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAAtttttt                                                               <  1:44523/150‑1 (MQ=255)
                                                                                        aGGAATGACCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCa                                                             <  1:619584/150‑1 (MQ=255)
                                                                                         ggAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAg                                                            >  2:478207/1‑150 (MQ=255)
                                                                                         ggAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATAACGTGCTGGCGAACAACTGGATCGAATTTTTTCAg                                                            >  1:500034/1‑150 (MQ=255)
                                                                                             tGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTcc                                                        <  2:656229/150‑1 (MQ=255)
                                                                                             tGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTcc                                                        <  1:1312187/150‑1 (MQ=255)
                                                                                               aaCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCTAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAg                                                      >  1:154159/1‑150 (MQ=255)
                                                                                               aaCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAg                                                      >  2:213799/1‑150 (MQ=255)
                                                                                                aCAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGt                                                     <  1:1183743/150‑1 (MQ=255)
                                                                                                 cAGATCAAGTCAATATGGGGGCAAGCAGATAACAAAAAACACCGCAGCAGCGAGGTATTTTGTTACATCAAACCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGtt                                                    <  2:37885/150‑1 (MQ=255)
                                                                                                   gATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGtttt                                                  >  1:1035588/1‑150 (MQ=255)
                                                                                                   gATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGtttt                                                  <  2:760433/150‑1 (MQ=255)
                                                                                                         gTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGc                                            >  2:466666/1‑150 (MQ=255)
                                                                                                         gTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGc                                            <  2:1272934/150‑1 (MQ=255)
                                                                                                                    gtcAAGCAGATAACAAAATACCCCGCCGGAGGGAGGTTTTTTGTTACATCATAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGATCAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGttt                                 <  1:1296831/148‑1 (MQ=255)
                                                                                                                        aGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGttc                             >  2:1300846/1‑150 (MQ=255)
                                                                                                                             aTAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGt                        <  1:1308603/150‑1 (MQ=255)
                                                                                                                               aaCAAAAAACCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAg                      >  2:720637/1‑150 (MQ=255)
                                                                                                                               aaCAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATGTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTat                      >  1:1171516/1‑149 (MQ=255)
                                                                                                                                aCAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGt                     <  1:1249765/150‑1 (MQ=255)
                                                                                                                                 cAAAAAACCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTg                    >  1:1160048/1‑150 (MQ=255)
                                                                                                                                 cAAAAAACCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTg                    >  2:1021449/1‑150 (MQ=255)
                                                                                                                                 cAAAAAACCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTg                    >  1:306979/1‑150 (MQ=255)
                                                                                                                                  aaaaaaCCTCGCTCCGGCGGGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg                   >  1:1189310/1‑150 (MQ=255)
                                                                                                                                  aaaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg                   <  2:427362/150‑1 (MQ=255)
                                                                                                                                  aaaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg                   <  2:490789/150‑1 (MQ=255)
                                                                                                                                  aaaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTgg                   <  2:1296606/150‑1 (MQ=255)
                                                                                                                                   aaaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGt                  <  2:1278166/150‑1 (MQ=255)
                                                                                                                                    aaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGta                 <  2:547735/150‑1 (MQ=255)
                                                                                                                                    aaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGta                 <  2:552543/150‑1 (MQ=255)
                                                                                                                                    aaaaCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGta                 <  2:182913/150‑1 (MQ=255)
                                                                                                                                       aCCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAg              <  1:1306112/150‑1 (MQ=255)
                                                                                                                                        ccccGCCGGAGCGAGGTTTTTTGTTTCATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACTGTTGTTCTTCGTAGTGGTATAGa             >  2:227874/1‑150 (MQ=255)
                                                                                                                                        ccccGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGa             <  1:1026818/150‑1 (MQ=255)
                                                                                                                                        ccccGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGATCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGa             <  1:213799/150‑1 (MQ=255)
                                                                                                                                          ccgcagGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAg           <  1:109627/150‑1 (MQ=255)
                                                                                                                                            gccgGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTg         >  1:1025535/1‑150 (MQ=255)
                                                                                                                                             ccgGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGa        >  1:1209497/1‑150 (MQ=255)
                                                                                                                                             ccgGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGa        >  2:431621/1‑150 (MQ=255)
                                                                                                                                               gtaGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGAcc      <  2:228523/148‑1 (MQ=255)
                                                                                                                                               ggAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGAcc      >  2:105080/1‑150 (MQ=255)
                                                                                                                                               ggAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGAcc      <  2:1052853/150‑1 (MQ=255)
                                                                                                                                                 aGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTGAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAg    <  2:398179/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTGCATGAAGAATGGTTGCACCAACGAGATGCGGTTCTATGCCGCGGCGGCTGGTTTCAACTTCGGGTAATTCAGGCATAGCATCTCCAGGAATGAACAGATGCAGTCAATATGGGGGCAAGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTTTTGTTACATCAAAGCGAGAATTATTTGATTTTCGCTTCTTTGTAGATCACGTGCTGGCGAACAACTGGATCGAATTTTTTCAGTTCCAGTTTTTCCGGCTTAGTACGTTTGTTCTTCGTAGTGGTATAGAAGTGACCAGTA  >  NC_000913/3811074‑3811370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: