Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,815,107 T→A 5.9% intergenic (+17/+20) slmA → / ← pyrE nucleoid occlusion factor, anti‑FtsZ division inhibitor/orotate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,1070TA 5.9% 302.9 / 4.7 108intergenic (+17/+20)slmA/pyrEnucleoid occlusion factor, anti‑FtsZ division inhibitor/orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (50/51);  new base A (2/5);  total (52/56)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 110 total aligned reads displayed.

ACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCAC  >  NC_000913/3814959‑3815254
                                                                                                                                                    |                                                                                                                                                   
aCCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACtt                                                                                                                                                    <  1:1069474/150‑1 (MQ=255)
  ccTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACtttt                                                                                                                                                  <  1:37399/150‑1 (MQ=255)
   ctgctgGCAAGCCAGTTCCTGGCCTTCTGTGAAGGTATGCTGTAACGTTTTGTCCGCTGCGATTTTAAATACCGCCCGACGGATGTTTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTc                                                                                                                                                 <  2:568669/150‑1 (MQ=255)
     gctgGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCAt                                                                                                                                               <  2:434698/150‑1 (MQ=255)
      ctgGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGCATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATc                                                                                                                                              >  1:1204673/1‑150 (MQ=255)
       gggCACGCCCGATCCTGGCCGTCTGTGAAGGGATGCTGTCACGGTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATcc                                                                                                                                             <  2:1088380/149‑1 (MQ=255)
       gggCAAGCCAGATCCTGGCCTTCTGAGAAGGTAGGCTGTCACGGTTTGTCCGCAGCGAATTTAAATACCGCCGGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATcc                                                                                                                                             <  2:1093727/149‑1 (MQ=255)
         gCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCgg                                                                                                                                           <  1:891769/150‑1 (MQ=255)
         gCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGAAAAGTCATCCgg                                                                                                                                           <  2:590320/150‑1 (MQ=255)
         gCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGAAAAGTCATCCgg                                                                                                                                           <  1:1297926/150‑1 (MQ=255)
          cAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGc                                                                                                                                          >  2:807991/1‑150 (MQ=255)
          cAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGc                                                                                                                                          >  1:776018/1‑150 (MQ=255)
          cAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGAAAAGTCATCCGGc                                                                                                                                          <  2:290973/150‑1 (MQ=255)
          cAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGAAAAGTCATCCGGc                                                                                                                                          <  1:882581/150‑1 (MQ=255)
             gCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTTATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCAGCCGGCGAg                                                                                                                                       >  1:1093270/1‑150 (MQ=255)
             gCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAg                                                                                                                                       >  2:975902/1‑150 (MQ=255)
             gCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAg                                                                                                                                       >  1:824080/1‑150 (MQ=255)
             gCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAg                                                                                                                                       >  1:805834/1‑150 (MQ=255)
             gCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAg                                                                                                                                       >  2:409363/1‑150 (MQ=255)
             gCCAGATCCTGGCATTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCTGCACAGTTGAAGTAATATGACGCCGGATGACTTTTCATCCGGCGAg                                                                                                                                       >  1:968192/1‑150 (MQ=255)
                aGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGttt                                                                                                                                    <  2:377762/150‑1 (MQ=255)
                       ggCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTaaa                                                                                                                             >  1:51093/1‑150 (MQ=255)
                        gCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAAc                                                                                                                            <  1:80778/150‑1 (MQ=255)
                        gCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAAc                                                                                                                            >  1:885560/1‑150 (MQ=255)
                         ccTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCTCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACg                                                                                                                           <  2:182939/150‑1 (MQ=255)
                               gtgAAGGTATGCTCTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAAc                                                                                                                     >  2:150903/1‑150 (MQ=255)
                                tgAAGGTATGCTGTCACGTTTTGTCCGCCGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCATAct                                                                                                                    >  1:620990/1‑150 (MQ=255)
                                    ggTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTGTCTTTAAACGCCAAACTCTTc                                                                                                                >  2:1262034/1‑150 (MQ=255)
                                     gTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTcg                                                                                                               >  2:1133677/1‑150 (MQ=255)
                                      gaTGCTGTCACGTTTTGTCCGCAGCGAATTTAACTACCGCCCGACGGATGCTTTTGACGCCCGCTGGCCGCTAATTGCGTCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTcgc                                                                                                              <  1:162765/149‑1 (MQ=255)
                                         gCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGAt                                                                                                           >  2:404183/1‑150 (MQ=255)
                                            gTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGCCTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAgg                                                                                                        >  1:631624/1‑150 (MQ=255)
                                             tCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGc                                                                                                       <  2:588353/150‑1 (MQ=255)
                                             tCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGc                                                                                                       <  1:565572/150‑1 (MQ=255)
                                              cACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGACAAACTCTTCGCGATAGGcc                                                                                                      >  2:1287178/1‑150 (MQ=255)
                                                 gTTATGTCCGCACCGAATATAAATACCGCCCCACGGATCATTTTGACGACCGCAGGCCGCAAATTGCGGCACAGGTGCAGAAAAATGACGACGGATGACATTTCATCCGGCGAGTTTCTTTAAACGCCAAGCTCTTCGCGATAGGCCTTa                                                                                                   >  1:273073/1‑150 (MQ=255)
                                                    ttGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAAcc                                                                                                >  2:805810/1‑150 (MQ=255)
                                                       tCCGCAGCGAATTTAAATACTGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAAccgcc                                                                                             <  1:368522/150‑1 (MQ=255)
                                                          gCAGCGAATTTAAATACCGCCCGACGGATGATTTTGCCGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCgccgcc                                                                                          >  1:169884/1‑150 (MQ=255)
                                                          gCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCgccgcc                                                                                          <  2:177998/150‑1 (MQ=255)
                                                          gCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCAGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCCTCCGGCGACGTTCTTTACACTCCAAACTCTTCGCGATAGGCCTTAACCgccgcc                                                                                          >  2:783734/1‑150 (MQ=255)
                                                              cGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCAAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGAt                                                                                      <  1:1228463/150‑1 (MQ=255)
                                                               gAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATg                                                                                     <  2:1153053/150‑1 (MQ=255)
                                                                aaTTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATCACTTTTCAACCGGCGAGTTTCTTTAAACGCCAAACTCTTCTCGATAGGCCTTAACCGCCGCCAGATGt                                                                                    >  1:871180/1‑150 (MQ=255)
                                                                 aTTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAGCTCTTCGCGATAGGCCTTAACCGCCGCCAGATGtt                                                                                   >  1:1159079/1‑150 (MQ=255)
                                                                  tttAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTc                                                                                  <  2:813986/150‑1 (MQ=255)
                                                                   ttAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGACGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGAATTTTCATCCGTCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCTCAGCCAGATGTTcc                                                                                 >  2:842408/1‑150 (MQ=255)
                                                                    tAAATACCGCCCGACGGATGATTTTGACGCGCGCTGGGCGATAATTGCGGCAGAGTTGCAGTAATATGACTCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGTTAGGCCTTAACCGCCGCCAGATGTTCCg                                                                                <  1:950946/150‑1 (MQ=255)
                                                                    tAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGAGGCACAGTTGCAGTATTATGACGCCGGATGACTTTTCATCCGGCGAGTTTCGTTAACCGCCAAACTCTTCGCGAGAGGCCTTAACCGCCGCCAGATGTGCCg                                                                                >  1:897435/1‑150 (MQ=255)
                                                                         aCCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCAtt                                                                           <  2:682608/150‑1 (MQ=255)
                                                                             cccGACGGATGATTTTGACTCCGGCTGGCCGCTAATTGCGTCACAGTGGGAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCATACTCTGCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCg                                                                       <  2:970282/150‑1 (MQ=255)
                                                                                     atgatgTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGAGGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTctct                                                               <  2:381813/150‑1 (MQ=255)
                                                                                      tgatTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCtt                                                              <  1:1303628/150‑1 (MQ=255)
                                                                                         ttttGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCa                                                           >  2:627093/1‑150 (MQ=255)
                                                                                            tGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCGTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGt                                                        >  1:142703/1‑150 (MQ=255)
                                                                                             gACGCCCGCTGGCCGCTAATTGCGGGACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTa                                                       <  1:50740/150‑1 (MQ=255)
                                                                                               cGCCCGCTGGCCGCTATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGc                                                    <  2:293677/150‑1 (MQ=255)
                                                                                                gCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGc                                                    >  1:640631/1‑150 (MQ=255)
                                                                                                 cccGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCCTCCGGCGAGTTTCTTTACACGCCAACCTCTTCGCGATAGGCCTAAACCGCCGCGAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGcc                                                   >  1:1215750/1‑149 (MQ=255)
                                                                                                   actgtgCCGCAATTAGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCTAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAAt                                                 >  1:230323/6‑150 (MQ=255)
                                                                                                     cTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCa                                               <  1:114030/150‑1 (MQ=255)
                                                                                                     cTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCa                                               >  1:265161/1‑150 (MQ=255)
                                                                                                     cTGGACGCTAATTGCGGCAAATTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCTTTTCCGGCTTCTCTTCCAGGTAAGAAATCa                                               <  2:1271239/150‑1 (MQ=255)
                                                                                                       ggCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAgg                                             <  1:1314500/150‑1 (MQ=255)
                                                                                                       ggCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAgg                                             <  1:7570/150‑1 (MQ=255)
                                                                                                       ggCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAgg                                             >  2:496447/1‑150 (MQ=255)
                                                                                                        gCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGt                                            >  1:928955/1‑150 (MQ=255)
                                                                                                         ccGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGta                                           >  2:1247175/1‑149 (MQ=255)
                                                                                                         ccGCAAATTGCGGCACAGTTGCAGAAATCTGACGCCCGATGACATTTCAACCGGCGAGTTTCTTTAAACGCCAAACTCATCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTc                                           <  1:795429/150‑1 (MQ=255)
                                                                                                          cGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCt                                          <  1:409363/150‑1 (MQ=255)
                                                                                                             tAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTc                                       <  1:713354/150‑1 (MQ=255)
                                                                                                              aaTTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCa                                      <  1:496669/150‑1 (MQ=255)
                                                                                                                ttGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAgg                                    <  1:400089/150‑1 (MQ=255)
                                                                                                                 tGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAggg                                   <  2:1108500/150‑1 (MQ=255)
                                                                                                                 tGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAggg                                   >  1:1203859/1‑150 (MQ=255)
                                                                                                                  gCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGt                                  >  2:906599/1‑150 (MQ=255)
                                                                                                                    ggCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGtga                                <  2:268061/150‑1 (MQ=255)
                                                                                                                           ttGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGAGATGATaga                         <  1:975902/150‑1 (MQ=255)
                                                                                                                            tgaaGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATagag                        <  1:526964/147‑1 (MQ=255)
                                                                                                                            tGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATagag                        >  1:741323/1‑150 (MQ=255)
                                                                                                                            tGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCAGCCATTTCCGGATTCTCTTCAAGGTAAGCAATCAGGTCTTTCAGGGTGAGGATagag                        >  2:809626/1‑150 (MQ=255)
                                                                                                                              cAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGAt                      >  1:1118287/1‑150 (MQ=255)
                                                                                                                                gTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCGTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGTGATCa                    <  2:169884/150‑1 (MQ=255)
                                                                                                                                 tAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCAc                   >  1:484758/1‑150 (MQ=255)
                                                                                                                                 tAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCAc                   <  2:7239/150‑1 (MQ=255)
                                                                                                                                  aaTATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACt                  >  1:534312/1‑150 (MQ=255)
                                                                                                                                  aaTATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACt                  <  2:907042/150‑1 (MQ=255)
                                                                                                                                   atatGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACtt                 >  1:976060/1‑150 (MQ=255)
                                                                                                                                     atGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTg               >  1:317372/1‑150 (MQ=255)
                                                                                                                                        tcGCCGGATGAAAAGTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCAGTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATTGAGATAACTTTGCAg            >  2:78784/2‑150 (MQ=255)
                                                                                                                                        aCGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAg            <  2:830460/150‑1 (MQ=255)
                                                                                                                                           ccGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGTTAGAGATCACTTTGCAGTTg         >  1:629741/1‑150 (MQ=255)
                                                                                                                                           ccGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTg         <  2:1056941/150‑1 (MQ=255)
                                                                                                                                           ccGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTg         <  1:1133677/150‑1 (MQ=255)
                                                                                                                                            cGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTACTTTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGAAGATAGAGATCACTTTGCAGTTGt        >  2:819506/1‑150 (MQ=255)
                                                                                                                                             ggATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCTTTTCCGGCTTCTCTTCCAGGTAAGCAATCACGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTa       >  1:639136/1‑150 (MQ=255)
                                                                                                                                             ggATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTa       <  2:51093/150‑1 (MQ=255)
                                                                                                                                              gATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTaa      <  2:776018/150‑1 (MQ=255)
                                                                                                                                                 gACTTTGCATCCGGCGAGGTTCTTTAAACGCCAAACTCTTCGCGATACGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGTTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCa   <  2:40807/150‑1 (MQ=255)
                                                                                                                                                  aCTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCGGGGTGTTGATAGAGATCACTTTGCAGTGGTatgccc  >  1:619625/1‑145 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCAGGGTGATGATAGAGATCACTTTGCAGTTGTAATCAC  >  NC_000913/3814959‑3815254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: