Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,832,188 A→T 5.1% intergenic (+22/+31) yicH → / ← yicI putative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,832,1880AT 5.1% 266.3 / 3.3 99intergenic (+22/+31)yicH/yicIputative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base A (42/50);  new base T (3/2);  total (47/52)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01

Only 100 of 103 total aligned reads displayed.

TCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCC  >  NC_000913/3832039‑3832337
                                                                                                                                                     |                                                                                                                                                     
tCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGa                                                                                                                                                       <  2:816167/150‑1 (MQ=255)
tCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAAAGCTGGCATCGTTTCCAGCGCTGAAGTTACAGCCACGGAGCCGGTGCGGTAAACATCAATCTCGCCTGGTACGa                                                                                                                                                       >  2:802490/1‑150 (MQ=255)
 cccGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGAc                                                                                                                                                      <  1:523863/150‑1 (MQ=255)
 cccGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGAc                                                                                                                                                      >  1:1055282/1‑150 (MQ=255)
 cccGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGAGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGAc                                                                                                                                                      <  2:1185715/150‑1 (MQ=255)
   cGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTc                                                                                                                                                    <  1:398812/150‑1 (MQ=255)
    gTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTAACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGAcacc                                                                                                                                                   >  2:809732/1‑147 (MQ=255)
      tGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACAGCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGa                                                                                                                                                 <  1:64768/150‑1 (MQ=255)
        aaaCCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACCTCAATCTCGCCGGGTACGACTCAGACg                                                                                                                                               >  1:899995/1‑150 (MQ=255)
        aaaCCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACg                                                                                                                                               <  2:1176711/150‑1 (MQ=255)
           ccTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCAGCGTTTCCAGCGGTGAAGTTAAATCGACGGAGCCGGTGCGGTAAACATCCATCTCGCCGGGTACGCATCAGAAGTAc                                                                                                                                            >  1:61910/1‑150 (MQ=255)
             aaCGGGTAGCGGGCAATGGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAccc                                                                                                                                          <  2:917091/149‑1 (MQ=255)
              aCGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCg                                                                                                                                         <  2:885412/150‑1 (MQ=255)
                ggTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCGGc                                                                                                                                       <  1:1011524/150‑1 (MQ=255)
                ggTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGc                                                                                                                                       >  1:1032540/1‑150 (MQ=255)
                  ttAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAt                                                                                                                                     <  1:83533/150‑1 (MQ=255)
                   tAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAtt                                                                                                                                    >  2:248123/1‑150 (MQ=255)
                   tAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAtt                                                                                                                                    <  1:1101541/150‑1 (MQ=255)
                     gCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGCCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCACTCTCGCCGGGTACGACTCAGCCGTCCCCGGCCTTcc                                                                                                                                  >  1:262637/1‑150 (MQ=255)
                     gCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTcc                                                                                                                                  <  2:170072/150‑1 (MQ=255)
                     gCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTcc                                                                                                                                  >  2:1102402/1‑150 (MQ=255)
                        ggCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                                                                               <  2:856702/150‑1 (MQ=255)
                        ggCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                                                                               >  1:553627/1‑150 (MQ=255)
                        ggCAATTCCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACGATGAAGGCTGGCAGCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                                                                               <  1:913952/150‑1 (MQ=255)
                         gCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCa                                                                                                                              >  1:567459/1‑150 (MQ=255)
                           aaTTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAt                                                                                                                            <  1:760656/150‑1 (MQ=255)
                           aaTTGCAGGCCGTGAACGCCGCAAAGAAGCAAGTGACTCAAACCATGAATGCTGTCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAAAATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAt                                                                                                                            <  1:821199/150‑1 (MQ=255)
                             ttGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCAGCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAg                                                                                                                          <  2:1200603/150‑1 (MQ=255)
                               gCATGCCGTGAACGCCGCAAAGCAGCAAGTGCCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCTATCTCGCCGGGTACGACTCAGACGTACCCGGCCTTCCATCAATAGAt                                                                                                                        >  2:605012/1‑150 (MQ=255)
                               gCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGAt                                                                                                                        >  1:1048610/1‑150 (MQ=255)
                                cATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGTGTACGACTCAGACGTACCCGGCATTCCATCAATAGAtt                                                                                                                       <  2:50368/150‑1 (MQ=255)
                                   gCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACa                                                                                                                    >  2:146626/1‑150 (MQ=255)
                                      gTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACg                                                                                                                 <  1:213701/150‑1 (MQ=255)
                                       tGAACGCCGCAAAGCAGCAAGTGACTCAATCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTTCAACGt                                                                                                                >  1:1217588/1‑150 (MQ=255)
                                       tGAACGCCGCAAAGCAGAAAGTGACTCAAAACATGAATGCTGGCATCGTTTCCAGCGGTGAACATACATCGAAGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGAACGACTCAGACGTACCCGCCATTCCATCAATAGATTACAACGt                                                                                                                >  2:985768/1‑150 (MQ=255)
                                          aCGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGCCTCAGACGTCCCCGGCATTCCATCAATAGATTACAACGTAAt                                                                                                             >  1:795635/1‑150 (MQ=255)
                                          aCGCCGCAAAGCAGCAAGTGACTAAAACCATTAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAAt                                                                                                             <  2:658807/150‑1 (MQ=255)
                                          aCGCCGAAAAGCAGCAAGTGACTCAAACCATGAATGCTGTCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAAt                                                                                                             <  2:638679/150‑1 (MQ=255)
                                            gccgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTg                                                                                                           >  1:93535/1‑150 (MQ=255)
                                             ccgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                                                                          <  2:215653/150‑1 (MQ=255)
                                             ccgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                                                                          >  1:885253/1‑150 (MQ=255)
                                                 aaaGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgc                                                                                                      <  2:351721/150‑1 (MQ=255)
                                                 aaaGCAGCAAGTGACTAAAACCATGAATGCTGGCATCGTTTACAGCGTTGAATTTACATCGACGGAGCCGGTGAGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgc                                                                                                      <  2:324343/150‑1 (MQ=255)
                                                   agcagcAAGAGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTCAAGTTACATCGACGGAGCCGGTGCGGTATAAATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGTTTACAACGTTATTGTCAgctc                                                                                                    >  1:540817/1‑148 (MQ=255)
                                                     cagcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCACCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACTTCAATCTCGCCGGGTACGACTCAGACGAACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCAt                                                                                                  >  1:5531/1‑150 (MQ=255)
                                                        caAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTcc                                                                                               >  2:1047350/1‑150 (MQ=255)
                                                         aaGTTACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTccc                                                                                              <  1:1238170/150‑1 (MQ=255)
                                                             gaatCAAACCATGAAAGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGa                                                                                          <  2:898119/147‑1 (MQ=255)
                                                             gACTCAAACCTTGAATGGTGGCATCGTTTCCAGCTGGGAAGTTACATGGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGCACGACTCAGCCGTACCCGGCATTCCATCAATAGATAACAACGTAATTGTCAGCGCATTCCCTTGa                                                                                          <  2:95244/150‑1 (MQ=255)
                                                              aCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAg                                                                                         <  2:547469/150‑1 (MQ=255)
                                                               cTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGCCGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACCACGTAATTGTCAGCGCATTCCCTTGAgg                                                                                        >  1:594902/1‑150 (MQ=255)
                                                               cTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGATACGACTAAGACGTACCCGGCATTCCATCAATAGATAACAACGTAATTTTCAGCTCATTACCTTGAgg                                                                                        >  2:256396/1‑150 (MQ=255)
                                                                tCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGc                                                                                       <  1:828655/150‑1 (MQ=255)
                                                                  aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCtt                                                                                     >  2:547080/1‑150 (MQ=255)
                                                                   aaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTc                                                                                    <  1:250614/150‑1 (MQ=255)
                                                                    aCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca                                                                                   <  1:144468/150‑1 (MQ=255)
                                                                         caccgCTGGAAACGATGCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTGgccacc                                                                              >  1:165871/5‑150 (MQ=255)
                                                                         caccgCTGGAAACGATGCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCaccacc                                                                              >  2:1199165/5‑150 (MQ=255)
                                                                          aaTGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCAccacca                                                                             <  2:85700/150‑1 (MQ=255)
                                                                            tGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGc                                                                           >  2:915407/1‑150 (MQ=255)
                                                                                 gCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACACCGTAATTGTCTGCGCATTCCCTTGAGGCTTACCCACCCGCCACTg                                                                      >  1:238428/1‑150 (MQ=255)
                                                                                 gCATCGTTTACAGCGGTGAAGTAACATCGACGGCGCCGGTGCGGTAAACATAAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCATGAGGCTTCACCACCAGCCACTg                                                                      >  2:14957/1‑150 (MQ=255)
                                                                                  cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGc                                                                     >  2:497491/1‑150 (MQ=255)
                                                                                  cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGc                                                                     <  2:553627/150‑1 (MQ=255)
                                                                                   aTCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCTATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCt                                                                    <  1:605012/150‑1 (MQ=255)
                                                                                   aTCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAGTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACAACCAGccccagat                                                                    >  2:807279/1‑146 (MQ=255)
                                                                                   aTCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTAAGACTCAGACGTACCCGGCATTCCCTCAATAGATTACACCGTAATTGTCAGCGCATTCCCGTGAGGCTTCCCCACCCGCCCCTGCt                                                                    >  2:811098/1‑150 (MQ=255)
                                                                                    tCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCAGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTc                                                                   <  2:894839/150‑1 (MQ=255)
                                                                                      gTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAAAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCAc                                                                 >  1:133919/1‑150 (MQ=255)
                                                                                            aGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGCGGCTTCACCACCAGCCCCTGCTCACGTTCAg                                                           >  1:344590/1‑150 (MQ=255)
                                                                                             gCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCTCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGc                                                          <  2:715523/150‑1 (MQ=255)
                                                                                             ccGGGGAAGTTACATCGACGGAGCCGGTGCGGCAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGc                                                          <  2:445306/149‑1 (MQ=255)
                                                                                              cGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGcc                                                         >  1:843262/1‑150 (MQ=255)
                                                                                                  gAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCg                                                     <  2:1032540/150‑1 (MQ=255)
                                                                                                  gAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCg                                                     <  2:757649/150‑1 (MQ=255)
                                                                                                   aaGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCAGCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGCGGCTTCACCACCAGCCCCTGCACACTTTCAGCCTGCgc                                                    >  1:130296/1‑149 (MQ=255)
                                                                                                    aGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCCCCACCCGCCCCTGCTCCCTTTCCGCCTGCGaa                                                   >  2:335731/1‑150 (MQ=255)
                                                                                                      ttACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAAcc                                                 <  1:1106052/150‑1 (MQ=255)
                                                                                                         cATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTc                                              <  1:386870/150‑1 (MQ=255)
                                                                                                          ttCGACGGCGCCGGTGCGGTAAACATCAATCTCGCCGGGTTCGAGTCAGACGTACCCGGCATTCCATCAATAGATTACACCGTAATTGTCTGCGCTTTCCGTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCt                                             <  1:809732/149‑1 (MQ=255)
                                                                                                           tCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGAACCCGGCATTCCAGCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCCCCACCAGCCCCGGCTCCCTGTCAGCCTGCGAACCGGCtg                                            >  2:844150/1‑149 (MQ=255)
                                                                                                             gACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTATTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGc                                          >  1:111238/1‑150 (MQ=255)
                                                                                                             gACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGc                                          <  1:367931/150‑1 (MQ=255)
                                                                                                                    ccGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGCCCAt                                   >  2:817946/1‑150 (MQ=255)
                                                                                                                      ggTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCAttt                                 <  1:1047350/150‑1 (MQ=255)
                                                                                                                        tGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTAc                               >  2:914566/1‑150 (MQ=255)
                                                                                                                          cGGTAAACATCAATCTCGCCGGGTACGACTCAGACGAACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACtt                             <  1:685507/150‑1 (MQ=255)
                                                                                                                           ggTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACttt                            >  2:762247/1‑150 (MQ=255)
                                                                                                                            gTAAAGATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCTGACCATTTACTTTc                           <  1:729196/150‑1 (MQ=255)
                                                                                                                             tAAACCTCAATCTCGCCGGGGACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATGCCCATGAGGCTGCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTca                          <  2:218036/150‑1 (MQ=255)
                                                                                                                                    cAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACAtt                   >  2:585623/1‑150 (MQ=255)
                                                                                                                                      aTCTCGCCGGGGACGACTCCGACGTACCCGGCATTCCATCAATAGCTTACAACGTAATTGTCAGCGCAGTCCCTTGAGGCTTCACCACCACCCCCTGCTCACTTTCCGCCTGCGAACCGGCTTGCAGACCATTTACTTTCACAACATTgc                 <  2:197877/150‑1 (MQ=255)
                                                                                                                                        ctcGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTgcgc               <  1:1102402/150‑1 (MQ=255)
                                                                                                                                          cGCCGGGTACGACTCAGACGTACCAGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGCGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTAGCAGACCATTTACTTTCACAACATTGCGCAg             >  1:1274944/1‑150 (MQ=255)
                                                                                                                                            ccGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAGCATTGCGCAGGc           >  2:31087/1‑150 (MQ=255)
                                                                                                                                              gggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGcac         <  1:248123/150‑1 (MQ=255)
                                                                                                                                               ggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGcaca        >  2:492245/1‑150 (MQ=255)
                                                                                                                                                  aCGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAgtg     <  1:915407/150‑1 (MQ=255)
                                                                                                                                                     aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTcc  <  2:899995/150‑1 (MQ=255)
                                                                                                                                                     aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTcc  <  2:720841/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCC  >  NC_000913/3832039‑3832337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: