Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,832,191 C→G 5.2% intergenic (+25/+28) yicH → / ← yicI putative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,832,1910CG 5.2% 292.0 / 7.2 97intergenic (+25/+28)yicH/yicIputative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base C (42/49);  new base G (3/2);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01

CGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCA  >  NC_000913/3832042‑3832338
                                                                                                                                                     |                                                                                                                                                   
cGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTc                                                                                                                                                     <  1:398812/150‑1 (MQ=255)
   tGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACAGCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGa                                                                                                                                                  <  1:64768/150‑1 (MQ=255)
     aaaCCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACCTCAATCTCGCCGGGTACGACTCAGACg                                                                                                                                                >  1:899995/1‑150 (MQ=255)
     aaaCCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACg                                                                                                                                                <  2:1176711/150‑1 (MQ=255)
        ccTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCAGCGTTTCCAGCGGTGAAGTTAAATCGACGGAGCCGGTGCGGTAAACATCCATCTCGCCGGGTACGCATCAGAAGTAc                                                                                                                                             >  1:61910/1‑150 (MQ=255)
          aaCGGGTAGCGGGCAATGGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAccc                                                                                                                                           <  2:917091/149‑1 (MQ=255)
           aCGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCg                                                                                                                                          <  2:885412/150‑1 (MQ=255)
             ggTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCGGc                                                                                                                                        <  1:1011524/150‑1 (MQ=255)
             ggTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGc                                                                                                                                        >  1:1032540/1‑150 (MQ=255)
               ttAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAt                                                                                                                                      <  1:83533/150‑1 (MQ=255)
                tAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAtt                                                                                                                                     <  1:1101541/150‑1 (MQ=255)
                tAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAtt                                                                                                                                     >  2:248123/1‑150 (MQ=255)
                  gCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGCCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCACTCTCGCCGGGTACGACTCAGCCGTCCCCGGCCTTcc                                                                                                                                   >  1:262637/1‑150 (MQ=255)
                  gCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTcc                                                                                                                                   <  2:170072/150‑1 (MQ=255)
                  gCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTcc                                                                                                                                   >  2:1102402/1‑150 (MQ=255)
                     ggCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                                                                                <  2:856702/150‑1 (MQ=255)
                     ggCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                                                                                >  1:553627/1‑150 (MQ=255)
                     ggCAATTCCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACGATGAAGGCTGGCAGCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                                                                                <  1:913952/150‑1 (MQ=255)
                      gCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCa                                                                                                                               >  1:567459/1‑150 (MQ=255)
                        aaTTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAt                                                                                                                             <  1:760656/150‑1 (MQ=255)
                        aaTTGCAGGCCGTGAACGCCGCAAAGAAGCAAGTGACTCAAACCATGAATGCTGTCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAAAATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAt                                                                                                                             <  1:821199/150‑1 (MQ=255)
                          ttGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCAGCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAg                                                                                                                           <  2:1200603/150‑1 (MQ=255)
                            gCATGCCGTGAACGCCGCAAAGCAGCAAGTGCCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCTATCTCGCCGGGTACGACTCAGACGTACCCGGCCTTCCATCAATAGAt                                                                                                                         >  2:605012/1‑150 (MQ=255)
                            gCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGAt                                                                                                                         >  1:1048610/1‑150 (MQ=255)
                             cATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGTGTACGACTCAGACGTACCCGGCATTCCATCAATAGAtt                                                                                                                        <  2:50368/150‑1 (MQ=255)
                                gCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACa                                                                                                                     >  2:146626/1‑150 (MQ=255)
                                   gTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACg                                                                                                                  <  1:213701/150‑1 (MQ=255)
                                    tGAACGCCGCAAAGCAGCAAGTGACTCAATCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTTCAACGt                                                                                                                 >  1:1217588/1‑150 (MQ=255)
                                    tGAACGCCGCAAAGCAGAAAGTGACTCAAAACATGAATGCTGGCATCGTTTCCAGCGGTGAACATACATCGAAGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGAACGACTCAGACGTACCCGCCATTCCATCAATAGATTACAACGt                                                                                                                 >  2:985768/1‑150 (MQ=255)
                                       aCGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGCCTCAGACGTCCCCGGCATTCCATCAATAGATTACAACGTAAt                                                                                                              >  1:795635/1‑150 (MQ=255)
                                       aCGCCGCAAAGCAGCAAGTGACTAAAACCATTAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAAt                                                                                                              <  2:658807/150‑1 (MQ=255)
                                       aCGCCGAAAAGCAGCAAGTGACTCAAACCATGAATGCTGTCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAAt                                                                                                              <  2:638679/150‑1 (MQ=255)
                                         gccgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTg                                                                                                            >  1:93535/1‑150 (MQ=255)
                                          ccgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                                                                           <  2:215653/150‑1 (MQ=255)
                                          ccgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                                                                           >  1:885253/1‑150 (MQ=255)
                                              aaaGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgc                                                                                                       <  2:351721/150‑1 (MQ=255)
                                              aaaGCAGCAAGTGACTAAAACCATGAATGCTGGCATCGTTTACAGCGTTGAATTTACATCGACGGAGCCGGTGAGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgc                                                                                                       <  2:324343/150‑1 (MQ=255)
                                               aaGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgcg                                                                                                      <  1:682088/150‑1 (MQ=255)
                                                agcagcAAGAGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTCAAGTTACATCGACGGAGCCGGTGCGGTATAAATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGTTTACAACGTTATTGTCAgctc                                                                                                     >  1:540817/1‑148 (MQ=255)
                                                  cagcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCACCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACTTCAATCTCGCCGGGTACGACTCAGACGAACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCAt                                                                                                   >  1:5531/1‑150 (MQ=255)
                                                     caAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTcc                                                                                                >  2:1047350/1‑150 (MQ=255)
                                                      aaGTTACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTccc                                                                                               <  1:1238170/150‑1 (MQ=255)
                                                          gaatCAAACCATGAAAGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGa                                                                                           <  2:898119/147‑1 (MQ=255)
                                                          gACTCAAACCTTGAATGGTGGCATCGTTTCCAGCTGGGAAGTTACATGGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGCACGACTCAGCCGTACCCGGCATTCCATCAATAGATAACAACGTAATTGTCAGCGCATTCCCTTGa                                                                                           <  2:95244/150‑1 (MQ=255)
                                                           aCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAg                                                                                          <  2:547469/150‑1 (MQ=255)
                                                            cTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGCCGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACCACGTAATTGTCAGCGCATTCCCTTGAgg                                                                                         >  1:594902/1‑150 (MQ=255)
                                                            cTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGATACGACTAAGACGTACCCGGCATTCCATCAATAGATAACAACGTAATTTTCAGCTCATTACCTTGAgg                                                                                         >  2:256396/1‑150 (MQ=255)
                                                             tCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGc                                                                                        <  1:828655/150‑1 (MQ=255)
                                                               aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCtt                                                                                      >  2:547080/1‑150 (MQ=255)
                                                                aaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTc                                                                                     <  1:250614/150‑1 (MQ=255)
                                                                 aCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca                                                                                    <  1:144468/150‑1 (MQ=255)
                                                                      caccgCTGGAAACGATGCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTGgccacc                                                                               >  1:165871/5‑150 (MQ=255)
                                                                      caccgCTGGAAACGATGCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCaccacc                                                                               >  2:1199165/5‑150 (MQ=255)
                                                                       aaTGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCAccacca                                                                              <  2:85700/150‑1 (MQ=255)
                                                                         tGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGc                                                                            >  2:915407/1‑150 (MQ=255)
                                                                              gCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACACCGTAATTGTCTGCGCATTCCCTTGAGGCTTACCCACCCGCCACTg                                                                       >  1:238428/1‑150 (MQ=255)
                                                                              gCATCGTTTACAGCGGTGAAGTAACATCGACGGCGCCGGTGCGGTAAACATAAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCATGAGGCTTCACCACCAGCCACTg                                                                       >  2:14957/1‑150 (MQ=255)
                                                                               cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGc                                                                      >  2:497491/1‑150 (MQ=255)
                                                                               cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGc                                                                      <  2:553627/150‑1 (MQ=255)
                                                                                aTCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCTATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCt                                                                     <  1:605012/150‑1 (MQ=255)
                                                                                aTCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAGTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACAACCAGccccagat                                                                     >  2:807279/1‑146 (MQ=255)
                                                                                aTCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTAAGACTCAGACGTACCCGGCATTCCCTCAATAGATTACACCGTAATTGTCAGCGCATTCCCGTGAGGCTTCCCCACCCGCCCCTGCt                                                                     >  2:811098/1‑150 (MQ=255)
                                                                                 tCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCAGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTc                                                                    <  2:894839/150‑1 (MQ=255)
                                                                                   gTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAAAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCAc                                                                  >  1:133919/1‑150 (MQ=255)
                                                                                         aGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGCGGCTTCACCACCAGCCCCTGCTCACGTTCAg                                                            >  1:344590/1‑150 (MQ=255)
                                                                                          gCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCTCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGc                                                           <  2:715523/150‑1 (MQ=255)
                                                                                          ccGGGGAAGTTACATCGACGGAGCCGGTGCGGCAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGc                                                           <  2:445306/149‑1 (MQ=255)
                                                                                           cGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGcc                                                          >  1:843262/1‑150 (MQ=255)
                                                                                               gAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCg                                                      <  2:757649/150‑1 (MQ=255)
                                                                                               gAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCg                                                      <  2:1032540/150‑1 (MQ=255)
                                                                                                aaGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCAGCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGCGGCTTCACCACCAGCCCCTGCACACTTTCAGCCTGCgc                                                     >  1:130296/1‑149 (MQ=255)
                                                                                                 aGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCCCCACCCGCCCCTGCTCCCTTTCCGCCTGCGaa                                                    >  2:335731/1‑150 (MQ=255)
                                                                                                   ttACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAAcc                                                  <  1:1106052/150‑1 (MQ=255)
                                                                                                      cATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTc                                               <  1:386870/150‑1 (MQ=255)
                                                                                                       ttCGACGGCGCCGGTGCGGTAAACATCAATCTCGCCGGGTTCGAGTCAGACGTACCCGGCATTCCATCAATAGATTACACCGTAATTGTCTGCGCTTTCCGTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCt                                              <  1:809732/149‑1 (MQ=255)
                                                                                                        tCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGAACCCGGCATTCCAGCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCCCCACCAGCCCCGGCTCCCTGTCAGCCTGCGAACCGGCtg                                             >  2:844150/1‑149 (MQ=255)
                                                                                                          gACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTATTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGc                                           >  1:111238/1‑150 (MQ=255)
                                                                                                          gACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGc                                           <  1:367931/150‑1 (MQ=255)
                                                                                                                 ccGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGCCCAt                                    >  2:817946/1‑150 (MQ=255)
                                                                                                                   ggTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCAttt                                  <  1:1047350/150‑1 (MQ=255)
                                                                                                                     tGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTAc                                >  2:914566/1‑150 (MQ=255)
                                                                                                                       cGGTAAACATCAATCTCGCCGGGTACGACTCAGACGAACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACtt                              <  1:685507/150‑1 (MQ=255)
                                                                                                                        ggTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACttt                             >  2:762247/1‑150 (MQ=255)
                                                                                                                         gTAAAGATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCTGACCATTTACTTTc                            <  1:729196/150‑1 (MQ=255)
                                                                                                                          tAAACCTCAATCTCGCCGGGGACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATGCCCATGAGGCTGCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTca                           <  2:218036/150‑1 (MQ=255)
                                                                                                                                tCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACAt                     <  2:352670/150‑1 (MQ=255)
                                                                                                                                 cAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACAtt                    >  2:585623/1‑150 (MQ=255)
                                                                                                                                   aTCTCGCCGGGGACGACTCCGACGTACCCGGCATTCCATCAATAGCTTACAACGTAATTGTCAGCGCAGTCCCTTGAGGCTTCACCACCACCCCCTGCTCACTTTCCGCCTGCGAACCGGCTTGCAGACCATTTACTTTCACAACATTgc                  <  2:197877/150‑1 (MQ=255)
                                                                                                                                     ctcGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTgcgc                <  1:1102402/150‑1 (MQ=255)
                                                                                                                                       cGCCGGGTACGACTCAGACGTACCAGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGCGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTAGCAGACCATTTACTTTCACAACATTGCGCAg              >  1:1274944/1‑150 (MQ=255)
                                                                                                                                        gCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAgg             >  2:988020/1‑150 (MQ=255)
                                                                                                                                         ccGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAGCATTGCGCAGGc            >  2:31087/1‑150 (MQ=255)
                                                                                                                                           gggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGcac          <  1:248123/150‑1 (MQ=255)
                                                                                                                                            ggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGcaca         >  2:492245/1‑150 (MQ=255)
                                                                                                                                               aCGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAgtg      <  1:915407/150‑1 (MQ=255)
                                                                                                                                                  aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTcc   <  2:720841/150‑1 (MQ=255)
                                                                                                                                                  aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTcc   <  2:899995/150‑1 (MQ=255)
                                                                                                                                                   cTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCa  >  1:1191567/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCA  >  NC_000913/3832042‑3832338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: