Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,832,194 A→T 5.2% intergenic (+28/+25) yicH → / ← yicI putative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,832,1940AT 5.2% 256.0 / 3.4 96intergenic (+28/+25)yicH/yicIputative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base A (42/47);  new base T (3/2);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01

TGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCA  >  NC_000913/3832045‑3832338
                                                                                                                                                     |                                                                                                                                                
tGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACAGCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGa                                                                                                                                                  <  1:64768/150‑1 (MQ=255)
  aaaCCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACCTCAATCTCGCCGGGTACGACTCAGACg                                                                                                                                                >  1:899995/1‑150 (MQ=255)
  aaaCCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACg                                                                                                                                                <  2:1176711/150‑1 (MQ=255)
     ccTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCAGCGTTTCCAGCGGTGAAGTTAAATCGACGGAGCCGGTGCGGTAAACATCCATCTCGCCGGGTACGCATCAGAAGTAc                                                                                                                                             >  1:61910/1‑150 (MQ=255)
       aaCGGGTAGCGGGCAATGGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAccc                                                                                                                                           <  2:917091/149‑1 (MQ=255)
        aCGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCg                                                                                                                                          <  2:885412/150‑1 (MQ=255)
          ggTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCGGc                                                                                                                                        <  1:1011524/150‑1 (MQ=255)
          ggTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGc                                                                                                                                        >  1:1032540/1‑150 (MQ=255)
            ttAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAt                                                                                                                                      <  1:83533/150‑1 (MQ=255)
             tAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAtt                                                                                                                                     <  1:1101541/150‑1 (MQ=255)
             tAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAtt                                                                                                                                     >  2:248123/1‑150 (MQ=255)
               gCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGCCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCACTCTCGCCGGGTACGACTCAGCCGTCCCCGGCCTTcc                                                                                                                                   >  1:262637/1‑150 (MQ=255)
               gCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTcc                                                                                                                                   <  2:170072/150‑1 (MQ=255)
               gCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTcc                                                                                                                                   >  2:1102402/1‑150 (MQ=255)
                  ggCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                                                                                <  2:856702/150‑1 (MQ=255)
                  ggCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                                                                                >  1:553627/1‑150 (MQ=255)
                  ggCAATTCCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACGATGAAGGCTGGCAGCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                                                                                <  1:913952/150‑1 (MQ=255)
                   gCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCa                                                                                                                               >  1:567459/1‑150 (MQ=255)
                     aaTTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAt                                                                                                                             <  1:760656/150‑1 (MQ=255)
                     aaTTGCAGGCCGTGAACGCCGCAAAGAAGCAAGTGACTCAAACCATGAATGCTGTCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAAAATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAt                                                                                                                             <  1:821199/150‑1 (MQ=255)
                       ttGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCAGCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAg                                                                                                                           <  2:1200603/150‑1 (MQ=255)
                         gCATGCCGTGAACGCCGCAAAGCAGCAAGTGCCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCTATCTCGCCGGGTACGACTCAGACGTACCCGGCCTTCCATCAATAGAt                                                                                                                         >  2:605012/1‑150 (MQ=255)
                         gCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGAt                                                                                                                         >  1:1048610/1‑150 (MQ=255)
                          cATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGTGTACGACTCAGACGTACCCGGCATTCCATCAATAGAtt                                                                                                                        <  2:50368/150‑1 (MQ=255)
                             gCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACa                                                                                                                     >  2:146626/1‑150 (MQ=255)
                                gTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACg                                                                                                                  <  1:213701/150‑1 (MQ=255)
                                 tGAACGCCGCAAAGCAGCAAGTGACTCAATCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTTCAACGt                                                                                                                 >  1:1217588/1‑150 (MQ=255)
                                 tGAACGCCGCAAAGCAGAAAGTGACTCAAAACATGAATGCTGGCATCGTTTCCAGCGGTGAACATACATCGAAGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGAACGACTCAGACGTACCCGCCATTCCATCAATAGATTACAACGt                                                                                                                 >  2:985768/1‑150 (MQ=255)
                                    aCGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGCCTCAGACGTCCCCGGCATTCCATCAATAGATTACAACGTAAt                                                                                                              >  1:795635/1‑150 (MQ=255)
                                    aCGCCGCAAAGCAGCAAGTGACTAAAACCATTAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAAt                                                                                                              <  2:658807/150‑1 (MQ=255)
                                    aCGCCGAAAAGCAGCAAGTGACTCAAACCATGAATGCTGTCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAAt                                                                                                              <  2:638679/150‑1 (MQ=255)
                                      gccgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTg                                                                                                            >  1:93535/1‑150 (MQ=255)
                                       ccgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                                                                           <  2:215653/150‑1 (MQ=255)
                                       ccgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                                                                           >  1:885253/1‑150 (MQ=255)
                                           aaaGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgc                                                                                                       <  2:351721/150‑1 (MQ=255)
                                           aaaGCAGCAAGTGACTAAAACCATGAATGCTGGCATCGTTTACAGCGTTGAATTTACATCGACGGAGCCGGTGAGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgc                                                                                                       <  2:324343/150‑1 (MQ=255)
                                            aaGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgcg                                                                                                      <  1:682088/150‑1 (MQ=255)
                                             agcagcAAGAGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTCAAGTTACATCGACGGAGCCGGTGCGGTATAAATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGTTTACAACGTTATTGTCAgctc                                                                                                     >  1:540817/1‑148 (MQ=255)
                                               cagcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCACCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACTTCAATCTCGCCGGGTACGACTCAGACGAACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCAt                                                                                                   >  1:5531/1‑150 (MQ=255)
                                                  caAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTcc                                                                                                >  2:1047350/1‑150 (MQ=255)
                                                   aaGTTACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTccc                                                                                               <  1:1238170/150‑1 (MQ=255)
                                                       gaatCAAACCATGAAAGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGa                                                                                           <  2:898119/147‑1 (MQ=255)
                                                       gACTCAAACCTTGAATGGTGGCATCGTTTCCAGCTGGGAAGTTACATGGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGCACGACTCAGCCGTACCCGGCATTCCATCAATAGATAACAACGTAATTGTCAGCGCATTCCCTTGa                                                                                           <  2:95244/150‑1 (MQ=255)
                                                        aCTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAg                                                                                          <  2:547469/150‑1 (MQ=255)
                                                         cTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGCCGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACCACGTAATTGTCAGCGCATTCCCTTGAgg                                                                                         >  1:594902/1‑150 (MQ=255)
                                                         cTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGATACGACTAAGACGTACCCGGCATTCCATCAATAGATAACAACGTAATTTTCAGCTCATTACCTTGAgg                                                                                         >  2:256396/1‑150 (MQ=255)
                                                          tCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGc                                                                                        <  1:828655/150‑1 (MQ=255)
                                                            aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCtt                                                                                      >  2:547080/1‑150 (MQ=255)
                                                             aaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTc                                                                                     <  1:250614/150‑1 (MQ=255)
                                                              aCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca                                                                                    <  1:144468/150‑1 (MQ=255)
                                                                   caccgCTGGAAACGATGCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTGgccacc                                                                               >  1:165871/5‑150 (MQ=255)
                                                                   caccgCTGGAAACGATGCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCaccacc                                                                               >  2:1199165/5‑150 (MQ=255)
                                                                    aaTGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCAccacca                                                                              <  2:85700/150‑1 (MQ=255)
                                                                      tGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGc                                                                            >  2:915407/1‑150 (MQ=255)
                                                                           gCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACACCGTAATTGTCTGCGCATTCCCTTGAGGCTTACCCACCCGCCACTg                                                                       >  1:238428/1‑150 (MQ=255)
                                                                           gCATCGTTTACAGCGGTGAAGTAACATCGACGGCGCCGGTGCGGTAAACATAAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCATGAGGCTTCACCACCAGCCACTg                                                                       >  2:14957/1‑150 (MQ=255)
                                                                            cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGc                                                                      >  2:497491/1‑150 (MQ=255)
                                                                            cATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGc                                                                      <  2:553627/150‑1 (MQ=255)
                                                                             aTCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCTATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCt                                                                     <  1:605012/150‑1 (MQ=255)
                                                                             aTCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAGTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACAACCAGccccagat                                                                     >  2:807279/1‑146 (MQ=255)
                                                                             aTCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTAAGACTCAGACGTACCCGGCATTCCCTCAATAGATTACACCGTAATTGTCAGCGCATTCCCGTGAGGCTTCCCCACCCGCCCCTGCt                                                                     >  2:811098/1‑150 (MQ=255)
                                                                              tCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCAGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTc                                                                    <  2:894839/150‑1 (MQ=255)
                                                                                gTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAAAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCAc                                                                  >  1:133919/1‑150 (MQ=255)
                                                                                      aGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGCGGCTTCACCACCAGCCCCTGCTCACGTTCAg                                                            >  1:344590/1‑150 (MQ=255)
                                                                                       gCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCTCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGc                                                           <  2:715523/150‑1 (MQ=255)
                                                                                       ccGGGGAAGTTACATCGACGGAGCCGGTGCGGCAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGc                                                           <  2:445306/149‑1 (MQ=255)
                                                                                        cGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGcc                                                          >  1:843262/1‑150 (MQ=255)
                                                                                            gAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCg                                                      <  2:757649/150‑1 (MQ=255)
                                                                                            gAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCg                                                      <  2:1032540/150‑1 (MQ=255)
                                                                                             aaGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCAGCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGCGGCTTCACCACCAGCCCCTGCACACTTTCAGCCTGCgc                                                     >  1:130296/1‑149 (MQ=255)
                                                                                              aGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCCCCACCCGCCCCTGCTCCCTTTCCGCCTGCGaa                                                    >  2:335731/1‑150 (MQ=255)
                                                                                                ttACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAAcc                                                  <  1:1106052/150‑1 (MQ=255)
                                                                                                   cATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTc                                               <  1:386870/150‑1 (MQ=255)
                                                                                                    ttCGACGGCGCCGGTGCGGTAAACATCAATCTCGCCGGGTTCGAGTCAGACGTACCCGGCATTCCATCAATAGATTACACCGTAATTGTCTGCGCTTTCCGTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCt                                              <  1:809732/149‑1 (MQ=255)
                                                                                                     tCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGAACCCGGCATTCCAGCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCCCCACCAGCCCCGGCTCCCTGTCAGCCTGCGAACCGGCtg                                             >  2:844150/1‑149 (MQ=255)
                                                                                                       gACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTATTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGc                                           >  1:111238/1‑150 (MQ=255)
                                                                                                       gACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGc                                           <  1:367931/150‑1 (MQ=255)
                                                                                                              ccGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGCCCAt                                    >  2:817946/1‑150 (MQ=255)
                                                                                                                ggTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCAttt                                  <  1:1047350/150‑1 (MQ=255)
                                                                                                                  tGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTAc                                >  2:914566/1‑150 (MQ=255)
                                                                                                                    cGGTAAACATCAATCTCGCCGGGTACGACTCAGACGAACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACtt                              <  1:685507/150‑1 (MQ=255)
                                                                                                                     ggTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACttt                             >  2:762247/1‑150 (MQ=255)
                                                                                                                      gTAAAGATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCTGACCATTTACTTTc                            <  1:729196/150‑1 (MQ=255)
                                                                                                                       tAAACCTCAATCTCGCCGGGGACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATGCCCATGAGGCTGCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTca                           <  2:218036/150‑1 (MQ=255)
                                                                                                                             tCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACAt                     <  2:352670/150‑1 (MQ=255)
                                                                                                                              cAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACAtt                    >  2:585623/1‑150 (MQ=255)
                                                                                                                                aTCTCGCCGGGGACGACTCCGACGTACCCGGCATTCCATCAATAGCTTACAACGTAATTGTCAGCGCAGTCCCTTGAGGCTTCACCACCACCCCCTGCTCACTTTCCGCCTGCGAACCGGCTTGCAGACCATTTACTTTCACAACATTgc                  <  2:197877/150‑1 (MQ=255)
                                                                                                                                  ctcGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTgcgc                <  1:1102402/150‑1 (MQ=255)
                                                                                                                                    cGCCGGGTACGACTCAGACGTACCAGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGCGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTAGCAGACCATTTACTTTCACAACATTGCGCAg              >  1:1274944/1‑150 (MQ=255)
                                                                                                                                     gCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAgg             >  2:988020/1‑150 (MQ=255)
                                                                                                                                      ccGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAGCATTGCGCAGGc            >  2:31087/1‑150 (MQ=255)
                                                                                                                                        gggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGcac          <  1:248123/150‑1 (MQ=255)
                                                                                                                                         ggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGcaca         >  2:492245/1‑150 (MQ=255)
                                                                                                                                            aCGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAgtg      <  1:915407/150‑1 (MQ=255)
                                                                                                                                               aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTcc   <  2:720841/150‑1 (MQ=255)
                                                                                                                                               aCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTcc   <  2:899995/150‑1 (MQ=255)
                                                                                                                                                cTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCa  >  1:1191567/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCA  >  NC_000913/3832045‑3832338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: