Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,906,830 G→A 6.1% intergenic (‑263/+23) bglG ← / ← phoU transcriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,906,8300GA 6.1% 299.2 / 6.4 99intergenic (‑263/+23)bglG/phoUtranscriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator
Reads supporting (aligned to +/- strand):  ref base G (45/47);  new base A (4/2);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01

ACGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACG  >  NC_000913/3906682‑3906978
                                                                                                                                                    |                                                                                                                                                    
aCGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGt                                                                                                                                                     >  2:753637/1‑150 (MQ=255)
aCGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGt                                                                                                                                                     >  2:832528/1‑150 (MQ=255)
aCGAACATCCAGGTTAGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGt                                                                                                                                                     <  1:478878/150‑1 (MQ=255)
  gAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTa                                                                                                                                                   >  1:1134805/1‑150 (MQ=255)
     cATCCAGGTTCTGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTg                                                                                                                                                <  1:79232/150‑1 (MQ=255)
     cATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTg                                                                                                                                                >  1:412382/1‑150 (MQ=255)
      aTCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTACCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCATTAATTATGTTATTgg                                                                                                                                               >  2:70966/1‑150 (MQ=255)
      aTCCAGGTTCGGAAATTAATTTAATTGAGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTgg                                                                                                                                               <  1:63902/150‑1 (MQ=255)
       tCCAGGTTCGGATATTAATTTACTTGCGTGCAGAGAAAATAGCATTGCGATATTGATAAAAATATGACCATGATCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCCATAATTAAGTTATTggg                                                                                                                                              <  1:61581/150‑1 (MQ=255)
        ccAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTGGGa                                                                                                                                             <  2:508948/150‑1 (MQ=255)
         cAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGAt                                                                                                                                            >  2:1136370/1‑150 (MQ=255)
         cAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGAt                                                                                                                                            <  1:696702/150‑1 (MQ=255)
          aGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGAtt                                                                                                                                           <  1:1242444/150‑1 (MQ=255)
           ggTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAG‑TATTGGGATTTg                                                                                                                                         <  2:352798/150‑1 (MQ=255)
            gTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTg                                                                                                                                         >  2:276694/1‑150 (MQ=255)
              tCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTTTTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGtt                                                                                                                                       <  2:380669/150‑2 (MQ=255)
               cGGAAATAAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATGGATAAAAATATGCCCATGCTCGCAGTTATTAACTTTTTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCACTAATTAAGTTATTGGGATTTGTCt                                                                                                                                      <  2:701607/150‑1 (MQ=255)
                ggAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTg                                                                                                                                     <  2:412382/150‑1 (MQ=255)
                ggAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTg                                                                                                                                     >  2:894400/1‑150 (MQ=255)
                ggAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTGGGATTTGttat                                                                                                                                     <  1:884960/150‑4 (MQ=255)
                 gAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTgg                                                                                                                                    <  1:115408/150‑1 (MQ=255)
                 gAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTgg                                                                                                                                    <  1:977705/150‑1 (MQ=255)
                   aattaattTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTg                                                                                                                                  >  2:287073/1‑150 (MQ=255)
                   aattaattTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCACAATAATTAAGTTATTGGGATTTGTCTGGTg                                                                                                                                  >  1:1066530/1‑150 (MQ=255)
                       aatttaatttCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAatt                                                                                                                              <  2:938877/150‑1 (MQ=255)
                        atttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAatta                                                                                                                             >  2:24965/1‑150 (MQ=255)
                            aattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTg                                                                                                                         <  2:544021/150‑1 (MQ=255)
                            aattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTg                                                                                                                         <  2:203499/150‑1 (MQ=255)
                              ttGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTc                                                                                                                       <  1:624576/150‑1 (MQ=255)
                              ttGAGTTCAGAGAAAATAGCAAAGCGCTATTGATAAAAATATGAACATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTc                                                                                                                       <  1:196641/150‑1 (MQ=255)
                                    gCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTAta                                                                                                                 >  2:248602/1‑149 (MQ=255)
                                     cAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGAGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGATTTATTTGTCGCTATCt                                                                                                                >  2:370143/1‑150 (MQ=255)
                                        agaAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTc                                                                                                             <  2:1181735/150‑1 (MQ=255)
                                        agaAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGATATCTTTc                                                                                                             >  2:81218/1‑150 (MQ=255)
                                         taaaaTAGCAATGATCTATTGATAAAAATATGACCATGCTCGCAGTTATTAAGTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTcc                                                                                                            <  2:728368/149‑1 (MQ=255)
                                          aaaaTAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTccc                                                                                                           <  1:645209/150‑1 (MQ=255)
                                          aaaaTAGCAATGCGATATTGATAAAAATATGAACTTTCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCAAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTccc                                                                                                           <  2:630474/150‑1 (MQ=255)
                                          aaaaTAGCAATGAGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTccc                                                                                                           <  1:649153/150‑1 (MQ=255)
                                             aTAGCAATGCGCTATTGATAAAAATATTACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGc                                                                                                        <  2:408245/150‑1 (MQ=255)
                                                   aTGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTTTCGCTATCTTTCCCCGCcagcag                                                                                                  >  1:636237/1‑150 (MQ=255)
                                                    tGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCTATATTTAAGTTATTGGGATTTGTCTGGTGATTTATTTGTCGCTATCTTTCCCAGCCAGCAGt                                                                                                 >  2:221634/1‑150 (MQ=255)
                                                     gcgcTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGTGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGtt                                                                                                >  2:276200/1‑150 (MQ=255)
                                                       gcTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTa                                                                                              >  1:292408/1‑150 (MQ=255)
                                                         tATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATc                                                                                            <  1:1212350/150‑1 (MQ=255)
                                                           ttGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCACAATAATTAATTTATTGTGATTTGTATGGTGAATTATTTGTCGCTATCTTTCAACGCAATCAGTTTATCCa                                                                                          >  1:961361/1‑150 (MQ=255)
                                                             gATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                                        <  2:229763/150‑1 (MQ=255)
                                                             gATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                                        >  2:95274/1‑150 (MQ=255)
                                                              aTAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCt                                                                                       <  1:92866/150‑1 (MQ=255)
                                                               tAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTGAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTc                                                                                      >  1:725310/1‑150 (MQ=255)
                                                                  aaaTATTACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCTATAATTAAGCTATTGGGATTTGTCTGGTTAATTATTTGTCTCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATc                                                                                   <  2:294577/150‑1 (MQ=255)
                                                                  aaaTATGTCCATGCTCGCAGTTATTATCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATc                                                                                   <  2:511712/150‑1 (MQ=255)
                                                                    atatGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTATGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGc                                                                                 <  2:240641/150‑1 (MQ=255)
                                                                    atatGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGc                                                                                 <  2:448399/150‑1 (MQ=255)
                                                                     tatGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGcc                                                                                <  2:718394/150‑1 (MQ=255)
                                                                     tatGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATAAAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGcc                                                                                <  1:499806/150‑1 (MQ=255)
                                                                     tatGACCATGCTCGAAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGTTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGcc                                                                                <  2:157788/150‑1 (MQ=255)
                                                                         aCCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCg                                                                            <  2:744449/150‑1 (MQ=255)
                                                                            aTGCTCGCAGTTATTACCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAAAAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACg                                                                         >  2:1000625/1‑150 (MQ=255)
                                                                            aTGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACg                                                                         <  2:904102/150‑1 (MQ=255)
                                                                             tGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGt                                                                        <  1:890706/150‑1 (MQ=255)
                                                                                 agCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACg                                                                    <  1:991744/149‑1 (MQ=255)
                                                                                  gCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTTGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACgg                                                                   <  1:1174693/150‑1 (MQ=255)
                                                                                  gCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGCCgg                                                                   >  2:840796/1‑150 (MQ=255)
                                                                                  gCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACgg                                                                   <  1:385242/150‑1 (MQ=255)
                                                                                   cAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGATTTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGa                                                                  >  2:705410/1‑150 (MQ=255)
                                                                                   cAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCACCGCCAGCAGTTTATCCAGCTCATCGACACCGACGTGACGGa                                                                  >  2:29679/1‑150 (MQ=255)
                                                                                        attaACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATcc                                                             >  2:552270/1‑150 (MQ=255)
                                                                                            aCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGcc                                                         <  1:273133/150‑1 (MQ=255)
                                                                                             cTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGACACAGACGTGACGGAAATCCTGccc                                                        >  1:236260/1‑150 (MQ=255)
                                                                                               ttGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCt                                                      <  1:480539/150‑1 (MQ=255)
                                                                                                        tttAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACgtagt                                             >  1:539887/1‑150 (MQ=255)
                                                                                                         ttAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACgtagta                                            <  1:954824/150‑1 (MQ=255)
                                                                                                                  tataAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATaaa                                   >  2:239985/1‑150 (MQ=255)
                                                                                                                    taAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACt                                 <  2:937664/150‑1 (MQ=255)
                                                                                                                      aaGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTca                               >  1:172750/1‑150 (MQ=255)
                                                                                                                       aGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTcac                              <  1:1178779/150‑1 (MQ=255)
                                                                                                                       aGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTcac                              <  2:589849/150‑1 (MQ=255)
                                                                                                                              tataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATAtt                       >  1:6884/1‑150 (MQ=255)
                                                                                                                              tataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATAtt                       >  2:897607/1‑150 (MQ=255)
                                                                                                                              tataACAAATCCCAATAAATAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATAtt                       >  2:1216209/1‑150 (MQ=255)
                                                                                                                               ataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACATATATTc                      >  1:637427/1‑150 (MQ=255)
                                                                                                                               ataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCAACGCCACAGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAGCTCACAAATATTc                      >  1:1270010/1‑150 (MQ=255)
                                                                                                                                taACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCt                     >  2:48260/1‑150 (MQ=255)
                                                                                                                                  aCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTgg                   <  2:1134805/150‑1 (MQ=255)
                                                                                                                                   cAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACTTAGTAGAAGATAAACTCACAAATATTCTGGc                  >  1:508650/1‑150 (MQ=255)
                                                                                                                                     aaTCCCAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAg                >  2:1259179/1‑150 (MQ=255)
                                                                                                                                      ataccAATAATTAAGTTATTGGGATTTGTCTGGAGAATTAATAGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGCAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATACTGGCAGc               >  2:463530/4‑150 (MQ=255)
                                                                                                                                        cccAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCCCAAATATTCTGGCAGCgg             >  2:230815/1‑150 (MQ=255)
                                                                                                                                        cccAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCgg             >  2:579726/1‑150 (MQ=255)
                                                                                                                                        cccAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCgg             >  1:362121/1‑150 (MQ=255)
                                                                                                                                        cccAATAACTTAATTATTGGGAGTTGTCTGGTGATTTATTTGTCGCTATCTTTCCACGACAGCAGTTTATACAGCTCATAGCCACCGACGTGCCGGAAATCCTGCCCCTTAACGTAGTAGAAGATAAACTCACAAAAATTCTGGCAGCgg             >  1:596713/1‑150 (MQ=255)
                                                                                                                                         ccAATAATTAAGTTATTGGGATTTGTTTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCCGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCggg            >  2:534085/1‑149 (MQ=255)
                                                                                                                                         ccAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCgga            >  1:1078898/1‑149 (MQ=255)
                                                                                                                                         ccAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCAAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTAACAAATATTCAGGCAGCGGt            >  1:500454/1‑150 (MQ=255)
                                                                                                                                             taatTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGcc        >  2:63329/1‑150 (MQ=255)
                                                                                                                                             taatTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGCTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGcc        <  2:1090211/150‑1 (MQ=255)
                                                                                                                                                 tAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATa    >  1:615048/1‑150 (MQ=255)
                                                                                                                                                  aaGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATAc   >  1:1304553/1‑150 (MQ=255)
                                                                                                                                                   aGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACg  >  2:541029/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATACG  >  NC_000913/3906682‑3906978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑