Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,911,819 T→G 5.2% intergenic (‑294/+20) pstS ← / ← glmS phosphate ABC transporter periplasmic binding protein/L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,911,8190TG 5.2% 260.7 / 2.6 93intergenic (‑294/+20)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (45/43);  new base G (3/2);  total (48/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

TATTCAGTCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCAC  >  NC_000913/3911670‑3911967
                                                                                                                                                     |                                                                                                                                                    
tatTCAGTCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCt                                                                                                                                                      <  1:1288087/150‑1 (MQ=255)
tatTCAGTCAAATACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCt                                                                                                                                                      <  2:1275929/150‑1 (MQ=255)
 atTCAGTCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCtt                                                                                                                                                     >  1:422814/1‑150 (MQ=255)
   tCAGTCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTAc                                                                                                                                                   <  2:833753/150‑1 (MQ=255)
   tCAGTCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTAc                                                                                                                                                   <  1:64413/150‑1 (MQ=255)
      gTCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCa                                                                                                                                                <  1:1134569/150‑1 (MQ=255)
      gTCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCa                                                                                                                                                <  1:1266764/150‑1 (MQ=255)
        ctaTTACAAACATTAATATCGAAGAGATGACAGAAAAATTTTCATTCTGTTACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAgg                                                                                                                                              <  1:254976/148‑1 (MQ=255)
         aaTTCCAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTACCCGTAGATGACTTTTTTTCACATGGAGTTGGCAGGATGGTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAggg                                                                                                                                             <  1:244335/150‑1 (MQ=255)
         aaTTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAggg                                                                                                                                             <  2:1289786/150‑1 (MQ=255)
         aaTTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGAGGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAggg                                                                                                                                             <  2:189934/150‑1 (MQ=255)
           ttACAAACATTAATAACGAAGAGATGACAGAAATATTTTCATTCTGTGACAGAGTAAAATTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAACTGCCCCGCTTACGCAGGGCa                                                                                                                                           <  1:72653/150‑1 (MQ=255)
            aaCAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGCAGTTGGCAGGATGTTTGATTAAAACCATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCAt                                                                                                                                          <  2:1265242/149‑1 (MQ=255)
             aCAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCTGCGTAAGCGGGGCAtt                                                                                                                                         <  2:851265/150‑2 (MQ=255)
             aCAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATc                                                                                                                                         >  1:239887/1‑150 (MQ=255)
              cAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACTGAGAAAAAGTAGCCGAAGATGACGGGTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATcc                                                                                                                                        >  1:646188/1‑150 (MQ=255)
              cAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATcc                                                                                                                                        <  1:286112/150‑1 (MQ=255)
              aaaaCATTAATAACGAAGAGATGACAGAAAAATTTTCATTCAGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATcc                                                                                                                                        <  1:1262821/149‑1 (MQ=255)
                  cATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCAttt                                                                                                                                    >  1:672461/1‑150 (MQ=255)
                    ttAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATttat                                                                                                                                  >  2:1035082/1‑150 (MQ=255)
                     taataaCGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATttatt                                                                                                                                 >  1:361099/1‑150 (MQ=255)
                        taaCGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACt                                                                                                                              <  1:361266/150‑1 (MQ=255)
                        aaaCGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACt                                                                                                                              <  2:1204830/149‑1 (MQ=255)
                         aaCGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTc                                                                                                                             >  1:706267/1‑150 (MQ=255)
                         aaCGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTc                                                                                                                             <  1:362803/150‑1 (MQ=255)
                           cGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCaa                                                                                                                           >  1:507016/1‑150 (MQ=255)
                            gAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAAc                                                                                                                          <  2:163681/150‑1 (MQ=255)
                              agagATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCg                                                                                                                        >  2:486974/1‑150 (MQ=255)
                                 gaTGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTaa                                                                                                                     >  1:1234834/1‑150 (MQ=255)
                                     aCAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGa                                                                                                                 >  2:1159242/1‑150 (MQ=255)
                                     aCAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGa                                                                                                                 >  1:709019/1‑150 (MQ=255)
                                       aGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGAtt                                                                                                               >  2:1261525/1‑150 (MQ=255)
                                        gAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATCTTTGATTAAAAAAATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATATATTACTCAACCGTAACAGAttt                                                                                                              >  2:1279305/1‑150 (MQ=255)
                                        gAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGAATGTCACATGGAGTTGGCAGTATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCAATTTATTACTCAACCGTAACCGAttt                                                                                                              >  2:1274298/1‑150 (MQ=255)
                                              ttttCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAg                                                                                                        <  2:1086697/150‑1 (MQ=255)
                                              ttttCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAg                                                                                                        <  1:602889/150‑1 (MQ=255)
                                              ttttCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAg                                                                                                        <  2:801504/150‑1 (MQ=255)
                                                      cTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCAATTATTACTCAACCGTAACCGATTTTGCCAGGTTAcgcg                                                                                                >  1:483382/1‑150 (MQ=255)
                                                         tgACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCt                                                                                             >  1:307472/1‑150 (MQ=255)
                                                         tgACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCt                                                                                             >  1:447260/1‑150 (MQ=255)
                                                           aCAGAGAAAAAGAAGCCGAAGATGACGGTATGTCACATGGAGTTGGCAGGATGTTTTATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTgg                                                                                           <  2:333034/150‑1 (MQ=255)
                                                            cAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGt                                                                                          >  1:1079247/1‑150 (MQ=255)
                                                             agagAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTc                                                                                         <  1:1079960/150‑1 (MQ=255)
                                                               agaAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCaa                                                                                       >  1:401518/1‑150 (MQ=255)
                                                                  aaaaGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTTTTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGGCAACGt                                                                                    >  1:865374/1‑150 (MQ=255)
                                                                      gTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGt                                                                                >  2:422133/1‑150 (MQ=255)
                                                                       tAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTg                                                                               <  2:875537/150‑1 (MQ=255)
                                                                       tAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTg                                                                               <  2:1040987/150‑1 (MQ=255)
                                                                         gCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGcc                                                                             <  2:1123369/150‑1 (MQ=255)
                                                                          ccGAACATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGAGTAAAAACATAACCGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCt                                                                            <  1:655337/150‑1 (MQ=255)
                                                                            gAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCttt                                                                          >  2:1148195/1‑150 (MQ=255)
                                                                                aTGACGGTTTGTCACATGGAGTGGGCAGGATGGTTGATTAAAAACATAACAGGAAGAAAACTGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGGTACGCGGCTGGTCAACGTCGGTGCCTTTGATc                                                                      <  2:1156877/150‑1 (MQ=255)
                                                                                   aCGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAgc                                                                   >  1:668919/1‑150 (MQ=255)
                                                                                   aCGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAgc                                                                   >  1:281403/1‑150 (MQ=255)
                                                                                         tGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACa                                                             <  1:744210/150‑1 (MQ=255)
                                                                                          gTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCAGCCATTTATTACTCCACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACAt                                                            >  1:753746/1‑150 (MQ=255)
                                                                                              caTGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAACTGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTa                                                        <  1:227346/150‑1 (MQ=255)
                                                                                              caTGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTa                                                        <  1:609780/150‑1 (MQ=255)
                                                                                               aTGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTaa                                                       <  2:991269/150‑1 (MQ=255)
                                                                                                tGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATGCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAg                                                      <  2:121965/150‑1 (MQ=255)
                                                                                                  gAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGcc                                                    >  1:1071850/1‑150 (MQ=255)
                                                                                                  gAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGcc                                                    <  1:1301734/150‑1 (MQ=255)
                                                                                                     ttGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCcagc                                                 <  1:755175/150‑1 (MQ=255)
                                                                                                      tGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCcagca                                                <  1:613923/150‑1 (MQ=255)
                                                                                                      tGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACACAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCcagca                                                >  2:39580/1‑150 (MQ=255)
                                                                                                       ggCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCACCCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCATTTGATCAGCTCGACATGGTAAGCcagcat                                               >  2:662140/1‑149 (MQ=255)
                                                                                                        gCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCagcagc                                              <  2:706267/150‑1 (MQ=255)
                                                                                                         cAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCt                                             >  1:1214096/1‑150 (MQ=255)
                                                                                                          aGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCATGGCATACATTTAATACTCAAAAGTAACCGATTTTACAATGTAACTCGGCTGGTCAAAGTCGGTGCCTTTTATCAGCGCAACATGGTAAGCCAGCAGCTg                                            >  1:221582/1‑150 (MQ=255)
                                                                                                              tGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGc                                        <  2:296217/150‑1 (MQ=255)
                                                                                                                   gATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCCTCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGCTCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAAc                                   >  1:879349/1‑150 (MQ=255)
                                                                                                                      tAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGgt                                >  1:688606/1‑150 (MQ=255)
                                                                                                                       aaaaaCATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGgtg                               >  2:260513/1‑150 (MQ=255)
                                                                                                                       aaaaaCATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCCCGACATGGTAACCCAGCAGCTGCAGCGGAACGgtg                               >  1:222261/1‑150 (MQ=255)
                                                                                                                                aCAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTACCCGATGTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCGGCGCGCCAAGGTAAGCCAGCAGCTGCAGCGGACCGGTGTAGAAGCTc                      >  1:209983/1‑150 (MQ=255)
                                                                                                                                aCAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATc                      <  1:1159242/150‑1 (MQ=255)
                                                                                                                                 cAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTACGCCAGCAGCTGCAGCGGAACGGTGTAGAAGTTCg                     >  2:1023020/1‑150 (MQ=255)
                                                                                                                                  aGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCgg                    >  2:322599/1‑150 (MQ=255)
                                                                                                                                   ggAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGt                   <  1:1261525/150‑1 (MQ=255)
                                                                                                                                    gaagaaAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGTTCGGTg                  >  2:264974/1‑150 (MQ=255)
                                                                                                                                    gaagaaAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTg                  <  2:765616/150‑1 (MQ=255)
                                                                                                                                    gaagaaAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGAAAGGTg                  >  2:295118/1‑150 (MQ=255)
                                                                                                                                     aagaaAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGc                 <  1:986837/150‑1 (MQ=255)
                                                                                                                                      agaaAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCa                >  1:168296/1‑150 (MQ=255)
                                                                                                                                       gaaAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCCACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCaa               >  1:646709/1‑150 (MQ=255)
                                                                                                                                          aaaTGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCTGCGGAACGGTGTAGAAGATCGGTGCAATcc            >  1:573311/1‑149 (MQ=255)
                                                                                                                                           gaTGCCCTGCGTAAGCGGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTag                            >  2:330559/2‑133 (MQ=255)
                                                                                                                                           gaTGCCCTGCGTAAGCGGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTag                            <  1:330559/132‑1 (MQ=255)
                                                                                                                                           gaTGCCCTGCGTAAGCGGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCAc           >  2:784425/2‑150 (MQ=255)
                                                                                                                                             tGCCCTGCGTAAGCGGGGCATCCATTTATTACTCACCCGTAACCGATTTTGCCAGGTTACGCGGCGGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGACCGGTGTAGAAGCTCGGTGGAATcccca         >  1:164487/1‑149 (MQ=255)
                                                                                                                                               ccccGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTa                                                        <  2:390916/101‑1 (MQ=255)
                                                                                                                                               ccccGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTa                                                        >  1:390916/1‑101 (MQ=255)
                                                                                                                                                  cGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTcc    <  1:1257771/150‑1 (MQ=255)
                                                                                                                                                  cGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTcc    >  1:729119/1‑150 (MQ=255)
                                                                                                                                                  cGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTcc    <  1:1035082/150‑1 (MQ=255)
                                                                                                                                                    cTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGAAATCACCTCTTCcac  >  2:669964/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TATTCAGTCAATTACAAACATTAATAACGAAGAGATGACAGAAAAATTTTCATTCTGTGACAGAGAAAAAGTAGCCGAAGATGACGGTTTGTCACATGGAGTTGGCAGGATGTTTGATTAAAAACATAACAGGAAGAAAAATGCCCCGCTTACGCAGGGCATCCATTTATTACTCAACCGTAACCGATTTTGCCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCAC  >  NC_000913/3911670‑3911967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: