Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,046,782 G→T 6.1% intergenic (‑180/‑184) yihG ← / → polA inner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,046,7820GT 6.1% 320.8 / 8.2 114intergenic (‑180/‑184)yihG/polAinner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease
Reads supporting (aligned to +/- strand):  ref base G (53/54);  new base T (2/5);  total (55/59)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

Only 100 of 118 total aligned reads displayed.

GATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCC  >  NC_000913/4046633‑4046931
                                                                                                                                                      |                                                                                                                                                      
taTTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTg                                                                                                                                                        <  2:397648/149‑1 (MQ=255)
gATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTg                                                                                                                                                        <  1:1101725/150‑1 (MQ=255)
 tttttAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGt                                                                                                                                                       <  2:248504/149‑1 (MQ=255)
  ttttAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTa                                                                                                                                                      <  2:295794/150‑1 (MQ=255)
     tAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATTAAAAACAAAGACAAACATCATGCTGTaaaa                                                                                                                                                   <  2:192631/150‑1 (MQ=255)
       tcAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAg                                                                                                                                                 <  1:886344/149‑1 (MQ=255)
       gCAGAAAACGACCCAAATAACGGATGATCCTTAAGGTGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAATAACAAAGACAAACATCATGCTGTATAAAg                                                                                                                                                 <  2:1090537/150‑1 (MQ=255)
         aGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCa                                                                                                                                               >  2:334645/1‑150 (MQ=255)
          taaaaCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCAt                                                                                                                                              <  1:744643/149‑1 (MQ=255)
           aaaaCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTAAATTATTAGGTATGGATTTATTTTTAACTGCATGAAAAAAAAAGAAAAACATCATGCTGTAAAAAGAATg                                                                                                                                             >  2:1176113/1‑150 (MQ=255)
             aaCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGat                                                                                                                                           <  2:721123/150‑1 (MQ=255)
              aCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatg                                                                                                                                          <  2:1004979/150‑2 (MQ=255)
              aCGAACCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGata                                                                                                                                          <  2:348156/150‑1 (MQ=255)
                gACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGataat                                                                                                                                        >  1:195555/1‑150 (MQ=255)
                caCCCAAATAACGTATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTAAAAGGGATGGATTTATTTTTAACTGCATGAAAAACACAGACAAACATCATGCTTTTTACAGCATGatgtt                                                                                                                                        <  1:185947/149‑4 (MQ=255)
                  cccAAATAACTGATTATCCTTAATGAGAAAAATAATTCATATCTATACACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatgtttg                                                                                                                                      <  2:622664/150‑6 (MQ=255)
                  cccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatgtttg                                                                                                                                      <  2:1314939/150‑6 (MQ=255)
                  cccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGAtaataa                                                                                                                                     >  1:371842/1‑150 (MQ=255)
                   ccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTATAAAAAATCGCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATaaa                                                                                                                                    <  2:826168/150‑1 (MQ=255)
                     aaaTAACTGATGATCCTTAATGAGAATAATAATTCATCTATAACCACATTAGAAAAAATCACATTATCTCAATTATTAGGGATGGATTTATTTATAACTGCAGGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAAtt                                                                                                                                  <  1:201097/150‑1 (MQ=255)
                     aaaTAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAAtt                                                                                                                                  >  2:1042058/1‑150 (MQ=255)
                      aaTAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTa                                                                                                                                 <  2:113725/150‑1 (MQ=255)
                         aaCGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTaaaa                                                                                                                              >  2:744475/1‑150 (MQ=255)
                         aaCGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTAAAAGGAAGTAAAACAAATGCAAACATTAATGCGTAAAAAGGATGATAATAAAATTaaaa                                                                                                                              >  1:467796/1‑150 (MQ=255)
                           cGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGc                                                                                                                            <  2:265747/150‑1 (MQ=255)
                            ggATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCg                                                                                                                           >  1:1042339/1‑150 (MQ=255)
                            ggATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCg                                                                                                                           >  2:649791/1‑150 (MQ=255)
                                gatCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGt                                                                                                                       >  2:882500/1‑150 (MQ=255)
                                 atCCTTAAGGAGTAAAATAATTCATATCTATCCAAATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACATACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTa                                                                                                                      <  1:827348/150‑1 (MQ=255)
                                  tCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTaa                                                                                                                     >  2:44841/1‑150 (MQ=255)
                                   ccTTAAGTATAAAAATAATTATTATCTATCAACATTAGAAAAAATACCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTaaa                                                                                                                    <  1:828053/150‑1 (MQ=255)
                                    cTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTTTTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATATTAAATTAAAAGCGATGTAaat                                                                                                                   >  2:482029/1‑150 (MQ=255)
                                    cTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAATAAGCATGATAATAAATTAAAAGCGATGTAaat                                                                                                                   >  2:122343/1‑150 (MQ=255)
                                    cTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAaat                                                                                                                   <  1:451651/150‑1 (MQ=255)
                                    attAAGGAGAATAATAATTCATATCTATCCAAATTAGAAAAAATCCCATTATCTCAATTATTAGGTATTGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAaat                                                                                                                   <  1:15005/149‑1 (MQ=255)
                                      tAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATAATTAGGGATTGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCTTGCTGTAAAAAGCATGATTATAAATTAAAAGCGATGTAaataa                                                                                                                 >  2:1075356/1‑150 (MQ=255)
                                           agaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTAt                                                                                                            <  2:961013/150‑1 (MQ=255)
                                            gaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCTATATCTCAATTATTATGTATGGATTTATTTTTAATTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATg                                                                                                           <  1:668748/150‑1 (MQ=255)
                                            gaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATg                                                                                                           >  2:479157/1‑150 (MQ=255)
                                            gaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATg                                                                                                           >  1:273702/1‑150 (MQ=255)
                                                aataatTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGcaca                                                                                                       >  1:567716/1‑150 (MQ=255)
                                                   aatTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACaaat                                                                                                    >  1:43523/1‑149 (MQ=255)
                                                         tatctatcCACATTAGAAAAACTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATATTTTATGCACAAAGTTATcc                                                                                              <  2:1116429/150‑1 (MQ=255)
                                                         tatctatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATcc                                                                                              <  1:406116/150‑1 (MQ=255)
                                                         tatctatcAACATTAGAAAAAATCACATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATTCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATcc                                                                                              <  2:1121614/150‑1 (MQ=255)
                                                          atctatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCca                                                                                             >  1:205218/1‑150 (MQ=255)
                                                           tctatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCcac                                                                                            <  2:195555/150‑1 (MQ=255)
                                                             tatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCcacaa                                                                                          >  2:294037/1‑149 (MQ=255)
                                                             tatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACAt                                                                                          >  2:963078/1‑150 (MQ=255)
                                                                 cacaTTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACg                                                                                      >  2:702801/1‑150 (MQ=255)
                                                                 cacaTTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACg                                                                                      >  2:1186572/1‑150 (MQ=255)
                                                                   caTTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGAt                                                                                    >  2:591846/1‑150 (MQ=255)
                                                                      tAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTa                                                                                                 <  1:340391/134‑1 (MQ=255)
                                                                          aaaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgag                                                                             <  2:520173/150‑1 (MQ=255)
                                                                          aaaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgag                                                                             <  1:633831/150‑1 (MQ=255)
                                                                           aaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgagc                                                                            <  1:1084007/150‑1 (MQ=255)
                                                                            aaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgagcg                                                                           >  2:151430/1‑150 (MQ=255)
                                                                              aTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATAATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCCCATGACGATTTGAGAGCGAt                                                                         >  2:352796/1‑150 (MQ=255)
                                                                                      aTCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCagaaga                                                                 >  2:280379/1‑150 (MQ=255)
                                                                                        ctcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATc                                                               >  2:887747/1‑150 (MQ=255)
                                                                                         tcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCt                                                              <  1:967055/150‑1 (MQ=255)
                                                                                         tcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCt                                                              <  1:629518/150‑1 (MQ=255)
                                                                                          cAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTa                                                             >  2:1221055/1‑150 (MQ=255)
                                                                                           aaTTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTAc                                                            <  1:284761/150‑1 (MQ=255)
                                                                                            attattAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACa                                                           <  1:891160/150‑1 (MQ=255)
                                                                                                        tgatTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCAc                                               <  1:649791/149‑1 (MQ=255)
                                                                                                        ggatTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCAc                                               >  1:345200/1‑150 (MQ=255)
                                                                                                         gatTTATTTTTAACTGCATGAAAAACAAAGCCAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATATATGCACAAAGTGATCCACATGACGATTTGCGAGCGCTCCAGAAGATCTACAAAAGATTTTCACg                                              >  2:205364/1‑150 (MQ=255)
                                                                                                             tatttTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGaaaa                                          >  1:397748/1‑150 (MQ=255)
                                                                                                               tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                        <  1:711944/150‑1 (MQ=255)
                                                                                                               tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                        <  2:482937/150‑1 (MQ=255)
                                                                                                               tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                        <  2:751640/150‑1 (MQ=255)
                                                                                                                    aaCTGCATGAAAAACAAAGACAAAAATCATGCTGTAAAAAGCATGATAATAAATTATAAGCGATGTAAATATTTTATGCAAAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAATAGATTTTCACGAAAAGCGGTGa                                   <  1:787335/150‑1 (MQ=255)
                                                                                                                      cTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGAGATGTAAATAATTTATGCACAAAGTTATCAACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaa                                 >  1:571038/1‑150 (MQ=255)
                                                                                                                       tGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaa                                >  2:1133248/1‑150 (MQ=255)
                                                                                                                       ggCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAAATAAAAGCGATGTAAATAATATATGCACAACGTTATCCACATGACGAATTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaa                                >  1:492551/2‑150 (MQ=255)
                                                                                                                        gCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaaa                               >  2:663929/1‑150 (MQ=255)
                                                                                                                            taaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCa                           <  1:629425/149‑1 (MQ=255)
                                                                                                                            gAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCa                           >  1:1096525/1‑150 (MQ=255)
                                                                                                                            gAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCAACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAAATCa                           >  2:37810/1‑150 (MQ=255)
                                                                                                                             aaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCAt                          >  2:789806/1‑150 (MQ=255)
                                                                                                                             aaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCAt                          >  2:1218057/1‑150 (MQ=255)
                                                                                                                                aaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGATGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGtt                       >  2:857926/1‑150 (MQ=255)
                                                                                                                                     aGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCAt                  <  2:108152/150‑1 (MQ=255)
                                                                                                                                     aGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGAAGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCAt                  <  2:104368/150‑1 (MQ=255)
                                                                                                                                       aCAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATcc                <  1:455483/150‑1 (MQ=255)
                                                                                                                                       aCAAACATCAAGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATATATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGACGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATcc                <  2:951587/150‑1 (MQ=255)
                                                                                                                                        cAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCct               >  1:10911/1‑150 (MQ=255)
                                                                                                                                           aCATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtc            <  1:871624/150‑1 (MQ=255)
                                                                                                                                            catcatGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtct           <  1:924280/150‑1 (MQ=255)
                                                                                                                                            catcatGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtct           <  1:397515/150‑1 (MQ=255)
                                                                                                                                             atcatGCTTTTTACAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtctg          >  1:401606/1‑150 (MQ=255)
                                                                                                                                               tcatGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtctgt         >  1:398690/1‑150 (MQ=255)
                                                                                                                                                 atGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATTACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTgg       <  2:354595/150‑1 (MQ=255)
                                                                                                                                                 atGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTgg       <  1:1113772/150‑1 (MQ=255)
                                                                                                                                                 atGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTgg       <  1:334645/150‑1 (MQ=255)
                                                                                                                                                  tGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGc      <  1:1186572/150‑1 (MQ=255)
                                                                                                                                                  tGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGc      <  1:702801/150‑1 (MQ=255)
                                                                                                                                                    cTGTAAAAAGTATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAATTTATCCAGTTGACTATTTGCTAGCGATCCAGAATATCTACTAAATATTTTCACTAAAAGCGGGGTAAAACTCATGTTTTCATCCTGTCTGTGTCAt    <  1:704635/150‑1 (MQ=255)
                                                                                                                                                      gTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATcc  <  2:516413/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GATTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCC  >  NC_000913/4046633‑4046931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: