Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,046,784 A→T 6.2% intergenic (‑182/‑182) yihG ← / → polA inner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,046,7840AT 6.2% 298.8 / 8.2 113intergenic (‑182/‑182)yihG/polAinner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease
Reads supporting (aligned to +/- strand):  ref base A (54/52);  new base T (2/5);  total (56/57)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.00e-01

Only 100 of 115 total aligned reads displayed.

TTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCC  >  NC_000913/4046635‑4046931
                                                                                                                                                      |                                                                                                                                                    
ttttAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTa                                                                                                                                                      <  2:295794/150‑1 (MQ=255)
   tAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATTAAAAACAAAGACAAACATCATGCTGTaaaa                                                                                                                                                   <  2:192631/150‑1 (MQ=255)
     tcAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAg                                                                                                                                                 <  1:886344/149‑1 (MQ=255)
     gCAGAAAACGACCCAAATAACGGATGATCCTTAAGGTGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAATAACAAAGACAAACATCATGCTGTATAAAg                                                                                                                                                 <  2:1090537/150‑1 (MQ=255)
       aGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCa                                                                                                                                               >  2:334645/1‑150 (MQ=255)
        taaaaCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCAt                                                                                                                                              <  1:744643/149‑1 (MQ=255)
         aaaaCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTAAATTATTAGGTATGGATTTATTTTTAACTGCATGAAAAAAAAAGAAAAACATCATGCTGTAAAAAGAATg                                                                                                                                             >  2:1176113/1‑150 (MQ=255)
           aaCGACCCAAATAACGTATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGat                                                                                                                                           <  1:907765/150‑1 (MQ=255)
           aaCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGat                                                                                                                                           <  2:721123/150‑1 (MQ=255)
            aCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatg                                                                                                                                          <  2:1004979/150‑2 (MQ=255)
              gACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGataat                                                                                                                                        >  1:195555/1‑150 (MQ=255)
              caCCCAAATAACGTATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTAAAAGGGATGGATTTATTTTTAACTGCATGAAAAACACAGACAAACATCATGCTTTTTACAGCATGatgtt                                                                                                                                        <  1:185947/149‑4 (MQ=255)
                cccAAATAACTGATTATCCTTAATGAGAAAAATAATTCATATCTATACACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatgtttg                                                                                                                                      <  2:622664/150‑6 (MQ=255)
                cccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatgtttg                                                                                                                                      <  2:1314939/150‑6 (MQ=255)
                cccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGAtaataa                                                                                                                                     >  1:371842/1‑150 (MQ=255)
                 ccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTATAAAAAATCGCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATaaa                                                                                                                                    <  2:826168/150‑1 (MQ=255)
                   aaaTAACTGATGATCCTTAATGAGAATAATAATTCATCTATAACCACATTAGAAAAAATCACATTATCTCAATTATTAGGGATGGATTTATTTATAACTGCAGGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAAtt                                                                                                                                  <  1:201097/150‑1 (MQ=255)
                   aaaTAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAAtt                                                                                                                                  >  2:1042058/1‑150 (MQ=255)
                    aaTAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTa                                                                                                                                 <  2:113725/150‑1 (MQ=255)
                       aaCGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTaaaa                                                                                                                              >  2:744475/1‑150 (MQ=255)
                       aaCGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTAAAAGGAAGTAAAACAAATGCAAACATTAATGCGTAAAAAGGATGATAATAAAATTaaaa                                                                                                                              >  1:467796/1‑150 (MQ=255)
                         cGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGc                                                                                                                            <  2:265747/150‑1 (MQ=255)
                          ggATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCg                                                                                                                           >  1:1042339/1‑150 (MQ=255)
                          ggATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCg                                                                                                                           >  2:649791/1‑150 (MQ=255)
                              gatCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGt                                                                                                                       >  2:882500/1‑150 (MQ=255)
                               atCCTTAAGGAGTAAAATAATTCATATCTATCCAAATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACATACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTa                                                                                                                      <  1:827348/150‑1 (MQ=255)
                               atCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAATAGCATGATAATAAATTAAAAGCGATGTa                                                                                                                      >  1:54432/1‑150 (MQ=255)
                                tCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTaa                                                                                                                     >  2:44841/1‑150 (MQ=255)
                                 ccTTAAGTATAAAAATAATTATTATCTATCAACATTAGAAAAAATACCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTaaa                                                                                                                    <  1:828053/150‑1 (MQ=255)
                                  cTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAATAAGCATGATAATAAATTAAAAGCGATGTAaat                                                                                                                   >  2:122343/1‑150 (MQ=255)
                                  cTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAaat                                                                                                                   <  1:451651/150‑1 (MQ=255)
                                    tAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATAATTAGGGATTGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCTTGCTGTAAAAAGCATGATTATAAATTAAAAGCGATGTAaataa                                                                                                                 >  2:1075356/1‑150 (MQ=255)
                                         agaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTAt                                                                                                            <  2:961013/150‑1 (MQ=255)
                                          gaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCTATATCTCAATTATTATGTATGGATTTATTTTTAATTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATg                                                                                                           <  1:668748/150‑1 (MQ=255)
                                          gaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATg                                                                                                           >  2:479157/1‑150 (MQ=255)
                                          gaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATg                                                                                                           >  1:273702/1‑150 (MQ=255)
                                              aataatTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGcaca                                                                                                       >  1:567716/1‑150 (MQ=255)
                                               ataatTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGCAAATAATTTATGCACaa                                                                                                      <  2:715633/150‑1 (MQ=255)
                                                 aatTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACaaat                                                                                                    >  1:43523/1‑149 (MQ=255)
                                                       tatctatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATcc                                                                                              <  1:406116/150‑1 (MQ=255)
                                                       tatctatcAACATTAGAAAAAATCACATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATTCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATcc                                                                                              <  2:1121614/150‑1 (MQ=255)
                                                        atctatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCca                                                                                             >  1:205218/1‑150 (MQ=255)
                                                         tctatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCcac                                                                                            <  2:195555/150‑1 (MQ=255)
                                                           tatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCcacaa                                                                                          >  2:294037/1‑149 (MQ=255)
                                                           tatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACAt                                                                                          >  2:963078/1‑150 (MQ=255)
                                                               cacaTTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACg                                                                                      >  2:702801/1‑150 (MQ=255)
                                                               cacaTTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACg                                                                                      >  2:1186572/1‑150 (MQ=255)
                                                               cacaTTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACg                                                                                      >  2:1251905/1‑150 (MQ=255)
                                                                 caTTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGAt                                                                                    >  2:591846/1‑150 (MQ=255)
                                                                    tAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTa                                                                                                 <  1:340391/134‑1 (MQ=255)
                                                                    tAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTa                                                                                                 >  2:340391/1‑134 (MQ=255)
                                                                        aaaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgag                                                                             <  1:633831/150‑1 (MQ=255)
                                                                        aaaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgag                                                                             <  2:520173/150‑1 (MQ=255)
                                                                         aaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgagc                                                                            <  1:1084007/150‑1 (MQ=255)
                                                                          aaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgagcg                                                                           >  2:151430/1‑150 (MQ=255)
                                                                            aTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATAATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCCCATGACGATTTGAGAGCGAt                                                                         >  2:352796/1‑150 (MQ=255)
                                                                              cccATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATcc                                                                       >  1:751640/1‑150 (MQ=255)
                                                                                    aTCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCagaaga                                                                 >  2:280379/1‑150 (MQ=255)
                                                                                      ctcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATc                                                               >  2:887747/1‑150 (MQ=255)
                                                                                      ctcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATGTGCGAGCGATCCAGAAGAta                                                               >  2:57595/1‑149 (MQ=255)
                                                                                       tcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCt                                                              <  1:629518/150‑1 (MQ=255)
                                                                                       tcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGAGAGCGATCCAGAAGATCt                                                              >  1:349195/1‑150 (MQ=255)
                                                                                        cAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTa                                                             >  2:1221055/1‑150 (MQ=255)
                                                                                         aaTTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTAc                                                            <  1:284761/150‑1 (MQ=255)
                                                                                          attattAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACa                                                           <  1:891160/150‑1 (MQ=255)
                                                                                                      tgatTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCAc                                               <  1:649791/149‑1 (MQ=255)
                                                                                                      ggatTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCAc                                               >  1:345200/1‑150 (MQ=255)
                                                                                                           tatttTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGaaaa                                          >  1:397748/1‑150 (MQ=255)
                                                                                                             tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                        <  2:482937/150‑1 (MQ=255)
                                                                                                             tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                        <  1:711944/150‑1 (MQ=255)
                                                                                                             tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                        <  2:751640/150‑1 (MQ=255)
                                                                                                                    cTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGAGATGTAAATAATTTATGCACAAAGTTATCAACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaa                                 >  1:571038/1‑150 (MQ=255)
                                                                                                                     tGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCGAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaa                                >  2:858572/1‑150 (MQ=255)
                                                                                                                     tGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaa                                >  2:1133248/1‑150 (MQ=255)
                                                                                                                      gCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACTTGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaaa                               >  2:410906/1‑150 (MQ=255)
                                                                                                                      gCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaaa                               <  1:271873/150‑1 (MQ=255)
                                                                                                                          taaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCa                           <  1:629425/149‑1 (MQ=255)
                                                                                                                          gAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCa                           >  1:1096525/1‑150 (MQ=255)
                                                                                                                          gAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCAACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAAATCa                           >  2:37810/1‑150 (MQ=255)
                                                                                                                           aaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCAt                          >  2:789806/1‑150 (MQ=255)
                                                                                                                              aaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGtt                       >  2:1135976/1‑150 (MQ=255)
                                                                                                                                   aGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCAt                  <  2:108152/150‑1 (MQ=255)
                                                                                                                                   aGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGAAGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCAt                  <  2:104368/150‑1 (MQ=255)
                                                                                                                                     aCAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAATATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATcc                <  2:225613/150‑1 (MQ=255)
                                                                                                                                     aCAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATcc                <  1:455483/150‑1 (MQ=255)
                                                                                                                                     aCAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATcc                <  2:105839/150‑1 (MQ=255)
                                                                                                                                     aCAAACATCAAGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATATATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGACGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATcc                <  2:951587/150‑1 (MQ=255)
                                                                                                                                      cAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCct               >  1:10911/1‑150 (MQ=255)
                                                                                                                                        attatcatGCTTTTTACAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgt             >  1:422096/4‑150 (MQ=255)
                                                                                                                                         aCATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtc            <  1:871624/150‑1 (MQ=255)
                                                                                                                                          catcatGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtct           <  1:924280/150‑1 (MQ=255)
                                                                                                                                          catcatGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtct           <  1:397515/150‑1 (MQ=255)
                                                                                                                                           atcatGCTTTTTACAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtctg          >  1:401606/1‑150 (MQ=255)
                                                                                                                                               atGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATTACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTgg       <  2:354595/150‑1 (MQ=255)
                                                                                                                                               atGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTgg       <  1:1113772/150‑1 (MQ=255)
                                                                                                                                                tGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGc      <  1:1186572/150‑1 (MQ=255)
                                                                                                                                                tGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGc      <  1:702801/150‑1 (MQ=255)
                                                                                                                                                  cTGTAAAAAGTATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAATTTATCCAGTTGACTATTTGCTAGCGATCCAGAATATCTACTAAATATTTTCACTAAAAGCGGGGTAAAACTCATGTTTTCATCCTGTCTGTGTCAt    <  1:704635/150‑1 (MQ=255)
                                                                                                                                                    gTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATcc  <  2:516413/150‑1 (MQ=255)
                                                                                                                                                    gTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGAACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATcc  >  1:1182500/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TTTTAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCC  >  NC_000913/4046635‑4046931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: