Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,046,785 A→T 6.7% intergenic (‑183/‑181) yihG ← / → polA inner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,046,7850AT 6.7% 321.9 / 9.5 113intergenic (‑183/‑181)yihG/polAinner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease
Reads supporting (aligned to +/- strand):  ref base A (54/51);  new base T (2/6);  total (56/57)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

Only 100 of 116 total aligned reads displayed.

TAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTT  >  NC_000913/4046638‑4046934
                                                                                                                                                    |                                                                                                                                                      
tAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATTAAAAACAAAGACAAACATCATGCTGTaaaa                                                                                                                                                      <  2:192631/150‑1 (MQ=255)
  gCAGAAAACGACCCAAATAACGGATGATCCTTAAGGTGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAATAACAAAGACAAACATCATGCTGTATAAAg                                                                                                                                                    <  2:1090537/150‑1 (MQ=255)
    aGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCa                                                                                                                                                  >  2:334645/1‑150 (MQ=255)
     taaaaCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCAt                                                                                                                                                 <  1:744643/149‑1 (MQ=255)
      aaaaCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTAAATTATTAGGTATGGATTTATTTTTAACTGCATGAAAAAAAAAGAAAAACATCATGCTGTAAAAAGAATg                                                                                                                                                >  2:1176113/1‑150 (MQ=255)
        aaCGACCCAAATAACGTATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGat                                                                                                                                              <  1:907765/150‑1 (MQ=255)
        aaCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGat                                                                                                                                              <  2:721123/150‑1 (MQ=255)
         aCGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatg                                                                                                                                             <  2:1004979/150‑2 (MQ=255)
         aCGAACCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGata                                                                                                                                             <  2:348156/150‑1 (MQ=255)
           gACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGataat                                                                                                                                           >  1:195555/1‑150 (MQ=255)
           caCCCAAATAACGTATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCCTTATCTCAATTAAAAGGGATGGATTTATTTTTAACTGCATGAAAAACACAGACAAACATCATGCTTTTTACAGCATGatgtt                                                                                                                                           <  1:185947/149‑4 (MQ=255)
             cccAAATAACTGATTATCCTTAATGAGAAAAATAATTCATATCTATACACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatgtttg                                                                                                                                         <  2:622664/150‑6 (MQ=255)
             cccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatgtttg                                                                                                                                         <  2:1314939/150‑6 (MQ=255)
             cccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGAtaataa                                                                                                                                        >  1:371842/1‑150 (MQ=255)
              ccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTATAAAAAATCGCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATaaa                                                                                                                                       <  2:826168/150‑1 (MQ=255)
                aaaTAACTGATGATCCTTAATGAGAATAATAATTCATCTATAACCACATTAGAAAAAATCACATTATCTCAATTATTAGGGATGGATTTATTTATAACTGCAGGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAAtt                                                                                                                                     <  1:201097/150‑1 (MQ=255)
                aaaTAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAAtt                                                                                                                                     >  2:1042058/1‑150 (MQ=255)
                 aaTAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTa                                                                                                                                    <  2:113725/150‑1 (MQ=255)
                    aaCGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTaaaa                                                                                                                                 >  2:744475/1‑150 (MQ=255)
                    aaCGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTAAAAGGAAGTAAAACAAATGCAAACATTAATGCGTAAAAAGGATGATAATAAAATTaaaa                                                                                                                                 >  1:467796/1‑150 (MQ=255)
                       ggATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCg                                                                                                                              >  1:1042339/1‑150 (MQ=255)
                       ggATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCg                                                                                                                              >  2:649791/1‑150 (MQ=255)
                           gatCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGt                                                                                                                          >  2:882500/1‑150 (MQ=255)
                            atCCTTAAGGAGTAAAATAATTCATATCTATCCAAATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACATACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTa                                                                                                                         <  1:827348/150‑1 (MQ=255)
                            atCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAATAGCATGATAATAAATTAAAAGCGATGTa                                                                                                                         >  1:54432/1‑150 (MQ=255)
                             tCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTaa                                                                                                                        >  2:44841/1‑150 (MQ=255)
                              ccTTAAGTATAAAAATAATTATTATCTATCAACATTAGAAAAAATACCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTaaa                                                                                                                       <  1:828053/150‑1 (MQ=255)
                               cTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTTTTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATATTAAATTAAAAGCGATGTAaat                                                                                                                      >  2:482029/1‑150 (MQ=255)
                               cTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAATAAGCATGATAATAAATTAAAAGCGATGTAaat                                                                                                                      >  2:122343/1‑150 (MQ=255)
                               cTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAaat                                                                                                                      <  1:451651/150‑1 (MQ=255)
                                 tAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATAATTAGGGATTGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCTTGCTGTAAAAAGCATGATTATAAATTAAAAGCGATGTAaataa                                                                                                                    >  2:1075356/1‑150 (MQ=255)
                                      agaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTAt                                                                                                               <  2:961013/150‑1 (MQ=255)
                                       gaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCTATATCTCAATTATTATGTATGGATTTATTTTTAATTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATg                                                                                                              <  1:668748/150‑1 (MQ=255)
                                       gaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATg                                                                                                              >  2:479157/1‑150 (MQ=255)
                                       gaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATg                                                                                                              >  1:273702/1‑150 (MQ=255)
                                           aataatTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGcaca                                                                                                          >  1:567716/1‑150 (MQ=255)
                                            ataatTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGCAAATAATTTATGCACaa                                                                                                         <  2:715633/150‑1 (MQ=255)
                                              aatTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACaaat                                                                                                       >  1:43523/1‑149 (MQ=255)
                                                    tatctatcCACATTAGAAAAACTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATATTTTATGCACAAAGTTATcc                                                                                                 <  2:1116429/150‑1 (MQ=255)
                                                    tatctatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATcc                                                                                                 <  1:406116/150‑1 (MQ=255)
                                                    tatctatcAACATTAGAAAAAATCACATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATTCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATcc                                                                                                 <  2:1121614/150‑1 (MQ=255)
                                                     atctatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCca                                                                                                >  1:205218/1‑150 (MQ=255)
                                                      tctatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCcac                                                                                               <  2:195555/150‑1 (MQ=255)
                                                        tatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCcacaa                                                                                             >  2:294037/1‑149 (MQ=255)
                                                        tatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACAt                                                                                             >  2:963078/1‑150 (MQ=255)
                                                            cacaTTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACg                                                                                         >  2:1186572/1‑150 (MQ=255)
                                                              caTTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGAt                                                                                       >  2:591846/1‑150 (MQ=255)
                                                                 tAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTa                                                                                                    >  2:340391/1‑134 (MQ=255)
                                                                 tAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTa                                                                                                    <  1:340391/134‑1 (MQ=255)
                                                                     aaaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgag                                                                                <  1:633831/150‑1 (MQ=255)
                                                                      aaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgagc                                                                               <  1:1084007/150‑1 (MQ=255)
                                                                       aaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgagcg                                                                              >  2:151430/1‑150 (MQ=255)
                                                                         aTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATAATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCCCATGACGATTTGAGAGCGAt                                                                            >  2:352796/1‑150 (MQ=255)
                                                                           cccATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATcc                                                                          >  1:751640/1‑150 (MQ=255)
                                                                                 aTCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCagaaga                                                                    >  2:280379/1‑150 (MQ=255)
                                                                                   ctcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATc                                                                  >  2:887747/1‑150 (MQ=255)
                                                                                   ctcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATGTGCGAGCGATCCAGAAGAta                                                                  >  2:57595/1‑149 (MQ=255)
                                                                                    tcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCt                                                                 <  1:967055/150‑1 (MQ=255)
                                                                                    tcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGAGAGCGATCCAGAAGATCt                                                                 >  1:349195/1‑150 (MQ=255)
                                                                                     cAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTa                                                                >  2:1221055/1‑150 (MQ=255)
                                                                                       attattAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACa                                                              <  1:891160/150‑1 (MQ=255)
                                                                                                   tgatTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCAc                                                  <  1:649791/149‑1 (MQ=255)
                                                                                                   ggatTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCAc                                                  >  1:345200/1‑150 (MQ=255)
                                                                                                    gatTTATTTTTAACTGCATGAAAAACAAAGCCAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATATATGCACAAAGTGATCCACATGACGATTTGCGAGCGCTCCAGAAGATCTACAAAAGATTTTCACg                                                 >  2:205364/1‑150 (MQ=255)
                                                                                                          tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                           <  2:482937/150‑1 (MQ=255)
                                                                                                          tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                           <  2:751640/150‑1 (MQ=255)
                                                                                                               aaCTGCATGAAAAACAAAGACAAAAATCATGCTGTAAAAAGCATGATAATAAATTATAAGCGATGTAAATATTTTATGCAAAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAATAGATTTTCACGAAAAGCGGTGa                                      <  1:787335/150‑1 (MQ=255)
                                                                                                                 cTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGAGATGTAAATAATTTATGCACAAAGTTATCAACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaa                                    >  1:571038/1‑150 (MQ=255)
                                                                                                                  tGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCGAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaa                                   >  2:858572/1‑150 (MQ=255)
                                                                                                                  tGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaa                                   >  2:1133248/1‑150 (MQ=255)
                                                                                                                  ggCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAAATAAAAGCGATGTAAATAATATATGCACAACGTTATCCACATGACGAATTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaa                                   >  1:492551/2‑150 (MQ=255)
                                                                                                                   gCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACTTGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaaa                                  >  2:410906/1‑150 (MQ=255)
                                                                                                                   gCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaaa                                  <  1:271873/150‑1 (MQ=255)
                                                                                                                   gCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaaa                                  >  2:663929/1‑150 (MQ=255)
                                                                                                                       taaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCa                              <  1:629425/149‑1 (MQ=255)
                                                                                                                       gAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCa                              >  1:1096525/1‑150 (MQ=255)
                                                                                                                       gAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCAACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAAATCa                              >  2:37810/1‑150 (MQ=255)
                                                                                                                        aaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCAt                             >  2:789806/1‑150 (MQ=255)
                                                                                                                        aaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCAt                             >  2:1218057/1‑150 (MQ=255)
                                                                                                                                aGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCAt                     <  2:108152/150‑1 (MQ=255)
                                                                                                                                  acacacATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATGTTTGCACAACGTTCTCCCCATGACGATGTGCGCGCGATCCAGCAGCTCTACAAAAGAGTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATcc                   <  1:657312/150‑1 (MQ=255)
                                                                                                                                  aCAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAATATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATcc                   <  2:225613/150‑1 (MQ=255)
                                                                                                                                  aCAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATcc                   <  2:105839/150‑1 (MQ=255)
                                                                                                                                  aCAAACATCAAGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATATATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGACGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATcc                   <  2:951587/150‑1 (MQ=255)
                                                                                                                                   cAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCct                  >  1:10911/1‑150 (MQ=255)
                                                                                                                                     attatcatGCTTTTTACAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgt                >  1:422096/4‑150 (MQ=255)
                                                                                                                                      aCATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtc               <  1:871624/150‑1 (MQ=255)
                                                                                                                                       catcatGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtct              <  1:397515/150‑1 (MQ=255)
                                                                                                                                       catcatGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtct              <  1:924280/150‑1 (MQ=255)
                                                                                                                                        atcatGCTTTTTACAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtctg             >  1:401606/1‑150 (MQ=255)
                                                                                                                                          tcatGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtctgt            >  1:398690/1‑150 (MQ=255)
                                                                                                                                            atGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTgg          <  1:334645/150‑1 (MQ=255)
                                                                                                                                            atGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTgg          <  1:1113772/150‑1 (MQ=255)
                                                                                                                                             tGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGc         <  1:1186572/150‑1 (MQ=255)
                                                                                                                                             tGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGc         <  1:702801/150‑1 (MQ=255)
                                                                                                                                               cTGTAAAAAGTATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAATTTATCCAGTTGACTATTTGCTAGCGATCCAGAATATCTACTAAATATTTTCACTAAAAGCGGGGTAAAACTCATGTTTTCATCCTGTCTGTGTCAt       <  1:704635/150‑1 (MQ=255)
                                                                                                                                                 gTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATcc     <  2:516413/150‑1 (MQ=255)
                                                                                                                                                 gTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGAACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATcc     >  1:1182500/1‑150 (MQ=255)
                                                                                                                                                    aaaaGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCttt  >  1:458043/1‑150 (MQ=255)
                                                                                                                                                    aaaaGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCttt  <  2:611140/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTT  >  NC_000913/4046638‑4046934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: