Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,232,564 G→T 9.2% P224T (CCG→ACG)  lysC ← lysine‑sensitive aspartokinase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,232,5640GT 9.2% 224.4 / 3.5 90P224T (CCG→ACG) lysClysine‑sensitive aspartokinase 3
Reads supporting (aligned to +/- strand):  ref base G (36/43);  new base T (8/2);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 4.96e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGA  >  NC_000913/4232416‑4232713
                                                                                                                                                    |                                                                                                                                                     
gggATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCggg                                                                                                                                                      >  2:822201/1‑150 (MQ=255)
       cGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCgg                                                                                                                                               <  1:290756/150‑1 (MQ=255)
        gCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGt                                                                                                                                              <  2:757707/150‑1 (MQ=255)
         cTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTc                                                                                                                                             >  1:1130346/1‑150 (MQ=255)
         cTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTc                                                                                                                                             >  1:166961/1‑150 (MQ=255)
         cTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGACAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGTACGTCGGTc                                                                                                                                             >  1:1027112/1‑150 (MQ=255)
          tGCGTACTGCGGTCAGCAACGTTGGCGGATGAAGTACTTTTGCACCAAAAGTTGCCAGCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTcc                                                                                                                                            <  2:236799/150‑1 (MQ=255)
           gCGTACGCCGGGTAGCAACGTTGCCGGATGCAGTCCTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCa                                                                                                                                           <  2:787548/150‑1 (MQ=255)
               aCTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGata                                                                                                                                       >  1:1150623/1‑150 (MQ=255)
               aCTGCGGGTAGCAAAGTTGCCGGTTGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCCCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGata                                                                                                                                       <  2:131584/150‑1 (MQ=255)
                cTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGCTTTCATCAATGCGTTTAGCTGCGGAAACTACGCGTGGATCGGAGGTGTAGAAGCCCGGGACGTCCGTCCAgcttt                                                                                                                                      >  2:89115/1‑146 (MQ=255)
                cTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGatat                                                                                                                                      <  2:595599/150‑1 (MQ=255)
                  gCGGGTAGCAACGTTGCCGGAGGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCa                                                                                                                                    <  1:640393/150‑1 (MQ=255)
                     ggTAGCAACTTAGCCGGATTCAGTACTTTTGCACCAAAAGGTTCCCTCTCTGCCGCTTAGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGTATCGGTGGTGTAGTTGGCCGTGACGTCGGTCCAGATTTCAaca                                                                                                                                 <  2:1001751/150‑1 (MQ=255)
                     ggTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAACAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAaca                                                                                                                                 <  2:635751/150‑1 (MQ=255)
                       tAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATCCCCGGGCACTCGGTCAAAATATAAaaaca                                                                                                                               >  2:746884/1‑149 (MQ=255)
                              gTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCg                                                                                                                        <  1:566859/150‑1 (MQ=255)
                              gTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCg                                                                                                                        <  1:436078/150‑1 (MQ=255)
                                tGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCgtg                                                                                                                      >  2:268734/1‑150 (MQ=255)
                                  ccGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGAGTGTa                                                                                                                    >  1:592140/1‑150 (MQ=255)
                                  ccGGAGGCAGGACTTTTGCACCAAAACTTGCCATCTCTGCCGCTGCGGCAAACGCTATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTa                                                                                                                    <  2:657385/150‑1 (MQ=255)
                                     gATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAg                                                                                                                 <  2:70859/150‑1 (MQ=255)
                                     gATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAg                                                                                                                 <  2:1083031/150‑1 (MQ=255)
                                     gATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAg                                                                                                                 <  2:792494/150‑1 (MQ=255)
                                      aTGCAGTACTTTTGCACCCAAACTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATTTCAACACGAGATGCGTGTAAAGc                                                                                                                <  2:337470/150‑1 (MQ=255)
                                        gCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCt                                                                                                              <  1:527060/150‑1 (MQ=255)
                                         cAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTc                                                                                                             <  2:667015/150‑1 (MQ=255)
                                          aGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTcc                                                                                                            <  1:853892/150‑1 (MQ=255)
                                             aCTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTTGTGTTTATTATGTTTAGGTGTGTACAGATATCTACACGAGATGCGTGTAAAGCCTCCGcc                                                                                                         <  2:723982/150‑1 (MQ=255)
                                               ttttGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGATACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAg                                                                                                       <  1:1223337/150‑1 (MQ=255)
                                                   gCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCTACTGCATCAATGCGTCTTGCTGCGGAAACTACGCGTGGATCGGGGGGGTAGAAGCCCGCGACGTCGGTCCAGATATCAACACGAGCTGCGTGTAAAGCCTCCGCCAGCAAg                                                                                                   <  1:1004420/150‑1 (MQ=255)
                                                    cACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAgg                                                                                                  >  1:859641/1‑150 (MQ=255)
                                                    cACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATCACCGTGAACTCCGTAAAAATAAAAAAAATAGATGAGTGTAAAGCCTCCGCCAGCAAgg                                                                                                  >  2:738215/1‑150 (MQ=255)
                                                     aCCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACACGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATCACCGTGAACTCCGTAAAGATAAAAACAATAGATGCGTGTAAAGCCTCCGCCAGCAAGGc                                                                                                 >  2:746174/1‑150 (MQ=255)
                                                      ccAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATCACCGTGAACTCGGTACATATATAAACAAGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCt                                                                                                >  2:751430/1‑150 (MQ=255)
                                                        aaaaGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGc                                                                                              >  1:332763/1‑150 (MQ=255)
                                                          aaGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCg                                                                                            <  2:767556/150‑1 (MQ=255)
                                                             ttGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGtat                                                                                         <  1:598804/150‑1 (MQ=255)
                                                             ttGCCATCTCTGCCCCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGTTCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGtat                                                                                         <  1:618687/150‑1 (MQ=255)
                                                                gcATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGGTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCTGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAAt                                                                                      <  1:66513/149‑1 (MQ=255)
                                                                ccATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCAAGCAAGGCTGCCGTATAAt                                                                                      >  1:749588/1‑150 (MQ=255)
                                                                   tctcTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGc                                                                                   <  2:429432/150‑1 (MQ=255)
                                                                   gctcgGCCGCTCCGGCCAACGCGATTGCATCATGGCGTTTTGCTGCCGCAAGTAGTCGGGGATCGGTGGTCTAGATTCACGGGACCTCGGTCCAGATATCATAACGAGATGCGTGTAAAGCCTCCGCCCGCAAGGCTGCGGTATAATCGc                                                                                   <  2:48195/149‑1 (MQ=255)
                                                                     tctGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTg                                                                                 >  1:690080/1‑150 (MQ=255)
                                                                       tGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGcc                                                                               <  1:746270/150‑1 (MQ=255)
                                                                         ccGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATCACCGGGACCTCGGTCAATATATAAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTc                                                                             >  2:311774/1‑150 (MQ=255)
                                                                         ccGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGAGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGCCTGCCGTATAATCGCTGCCTc                                                                             >  1:322300/1‑150 (MQ=255)
                                                                           gCTTCGGCAAACGAGATTTCATCAATGCGTTTTGCTGCTGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCa                                                                           <  2:902092/150‑1 (MQ=255)
                                                                            cTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTcccc                                                                          >  2:680107/1‑148 (MQ=255)
                                                                             ttCTGCAAACGCGATTTCATCATTGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACg                                                                         <  2:703890/150‑1 (MQ=255)
                                                                              tCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACgg                                                                        >  1:378320/1‑150 (MQ=255)
                                                                                ggCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGcc                                                                      >  1:13375/1‑150 (MQ=255)
                                                                                   aaaCGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGGGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAg                                                                   <  1:72114/150‑1 (MQ=255)
                                                                                    aaCGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGAGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGc                                                                  >  2:1124941/1‑150 (MQ=255)
                                                                                     aCGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGTGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCCCGGCAAAGcg                                                                 >  2:28763/1‑150 (MQ=255)
                                                                                     aCGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACTGCCAAGcg                                                                 >  1:950589/1‑150 (MQ=255)
                                                                                       tctaTTACATCAATGCGTTTGGCTGCCGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATTTCAACTCGAGATGCGTGTAAAGCCTGCGCCAGCAAGGCTGCCGTATAATCCCTGCCTCCACGGCCAAGcgtc                                                               <  2:50145/147‑1 (MQ=255)
                                                                                         gATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGcgtcgt                                                             <  2:337435/150‑1 (MQ=255)
                                                                                           tttCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTg                                                           <  1:1110302/150‑1 (MQ=255)
                                                                                             tcatcaTTGCGTTTTGCTGCGGAATCTACGCGGGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTa                                                         <  1:289872/150‑1 (MQ=255)
                                                                                             tcatcaATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTa                                                         <  1:359307/150‑1 (MQ=255)
                                                                                              catcaATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGTGAAGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTAc                                                        >  2:331177/1‑150 (MQ=255)
                                                                                                  aaTGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGAcc                                                    >  1:517758/1‑150 (MQ=255)
                                                                                                  aaTGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGAcc                                                    <  2:544309/150‑1 (MQ=255)
                                                                                                    tGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCtt                                                  >  1:185713/1‑150 (MQ=255)
                                                                                                     gCGTTTTGCTGCGGAAACTACTCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCttt                                                 >  1:564123/1‑150 (MQ=255)
                                                                                                      cGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGACCGTTAACTCCGTAAAAATATAAAAAAAATATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCACGCGTCGTTGTACGAGCtttt                                                >  2:41757/1‑149 (MQ=255)
                                                                                                           tgctgcGGAAACTACGCCTCGATCGGTGGAGTAGATGCCCGGGACGTCGGTCCAGATTTGAACACGAGATGCGTGTAATGCCTCCGCCAGCAAGGCTGCCGTAAAATCGCTGCCTCCACGGCCTAGCGTCGTTGTACGTCCTTTATATTc                                           <  2:1070969/150‑1 (MQ=255)
                                                                                                            gctgcGGAAACTCCGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTACAGCCTCCGCCAGCAAGGCTGACGTTTAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACAGTTATTGTCg                                          >  1:118264/1‑150 (MQ=255)
                                                                                                             ctgcGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCCGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTAAGACCTTTATTGTCGc                                         >  1:886933/1‑150 (MQ=255)
                                                                                                               gcGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCTTGAAGTCTGTCAAGATATCAACACGAGATGAGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTTCCTCCACGGCCAAGCGTCGTGGGACGACGTTTATTTTCGCtg                                       >  2:638438/1‑149 (MQ=255)
                                                                                                               gcGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTa                                       >  2:1258788/1‑150 (MQ=255)
                                                                                                               gcGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTa                                       >  1:1103923/1‑150 (MQ=255)
                                                                                                                 ggAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTAcc                                     <  1:123679/150‑1 (MQ=255)
                                                                                                                     aCTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATa                                 <  1:902825/150‑1 (MQ=255)
                                                                                                                       tACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAAAAAGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCTAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATaaa                               >  1:340319/1‑150 (MQ=255)
                                                                                                                        aCGCGTGGATCGGTGGTGTAGATCACCGTGAACTCCGTAAAAATAAAAAAAAAAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGGCCGTTATGTTCGCTACCGATAAAt                              >  2:889224/1‑150 (MQ=255)
                                                                                                                         cgcgTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCCGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATc                             >  1:1251709/1‑150 (MQ=255)
                                                                                                                         cgcgTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATc                             >  1:379923/1‑150 (MQ=255)
                                                                                                                             tGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATGACTCCCTg                         >  1:568955/1‑150 (MQ=255)
                                                                                                                               gATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGGCAAGCGTCGTTGTCCGACCTTTATTTGCGCTACCGGTAAATCCCTggg                       >  1:524018/1‑150 (MQ=255)
                                                                                                                                 tCGGTGGGGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTATAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTg                     <  1:555916/150‑1 (MQ=255)
                                                                                                                                     gggtgTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCa                 <  2:860951/149‑1 (MQ=255)
                                                                                                                                     gggtgTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCa                 <  2:446091/149‑1 (MQ=255)
                                                                                                                                         gtAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTaa             <  1:822201/150‑1 (MQ=255)
                                                                                                                                               gCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTc       <  2:735967/150‑1 (MQ=255)
                                                                                                                                                cccGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCa      >  2:1116984/1‑150 (MQ=255)
                                                                                                                                                cccGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCa      >  1:330705/1‑150 (MQ=255)
                                                                                                                                                cccGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCa      >  1:330875/1‑150 (MQ=255)
                                                                                                                                                cccGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGGTGTACGCCCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCCCTAAGCCTTCa      >  1:701625/1‑150 (MQ=255)
                                                                                                                                                 ccGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCAt     >  2:1227032/1‑150 (MQ=255)
                                                                                                                                                   gggACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTg   <  2:918837/150‑1 (MQ=255)
                                                                                                                                                    ggACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAATTCCCTGGGTGATCACTAAGCCTTCATTgc  >  2:1003747/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGGATATCGCTGCGTACTGCGGGTAGCAACGTTGCCGGATGCAGTACTTTTGCACCAAAAGTTGCCATCTCTGCCGCTTCGGCAAACGCGATTTCATCAATGCGTTTTGCTGCGGAAACTACGCGTGGATCGGTGGTGTAGATGCCCGGGACGTCGGTCCAGATATCAACACGAGATGCGTGTAAAGCCTCCGCCAGCAAGGCTGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGA  >  NC_000913/4232416‑4232713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑