Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,245,108 T→G 5.2% intergenic (‑33/+121) malF ← / ← malE maltose transporter subunit/maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,245,1080TG 5.2% 279.3 / 3.0 97intergenic (‑33/+121)malF/malEmaltose transporter subunit/maltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (42/49);  new base G (2/4);  total (44/53)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTCCCCTTGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTC  >  NC_000913/4244959‑4245255
                                                                                                                                                     |                                                                                                                                                   
ttCCCCTTGTGCGTACATTAAAACAACAAGGTACCCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCt                                                                                                                                                     >  2:165688/1‑150 (MQ=255)
ttCCCCTTGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCt                                                                                                                                                     >  1:919876/1‑150 (MQ=255)
ttCCCCTTGTGCGTACATTAAAACAACAAGGTAACCCACCAGAAGGCCTATCAGACCTAGCACTGACCATTTCAGCGCGTAGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCt                                                                                                                                                     <  1:33843/150‑1 (MQ=255)
   cccTTGTGCGTACATTAAAACAACAAGGTACCCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTAAATGACATCCATGGTGTTCTTCCTCATTCCAGGACGGATAAGGCTTTc                                                                                                                                                  >  1:425074/1‑150 (MQ=255)
   cccTTGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTc                                                                                                                                                  >  2:406911/1‑150 (MQ=255)
    ccTTGTGCGTACAATAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCa                                                                                                                                                 <  2:1047526/150‑1 (MQ=255)
         tgCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCt                                                                                                                                            <  2:517312/150‑1 (MQ=255)
         tgCGTAAAATAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGGTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCt                                                                                                                                            <  2:73730/150‑1 (MQ=255)
          gCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCtt                                                                                                                                           >  2:693723/1‑150 (MQ=255)
           cGTACATTAAAACACCAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGCTAAGGCTTTCACGCCTTa                                                                                                                                          >  2:1110881/1‑150 (MQ=255)
            gTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTAt                                                                                                                                         >  2:728806/1‑150 (MQ=255)
             tACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                        >  1:1235801/1‑150 (MQ=255)
             tACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                        <  2:359800/150‑1 (MQ=255)
             tACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTATc                                                                                                                                        <  1:496517/150‑1 (MQ=255)
              aCATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCACATTCCAGGACGGATAAGGCTTTTACGCCTTATcc                                                                                                                                       <  1:1210943/150‑1 (MQ=255)
               cATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTATCACTGACCATTTCCGCGCGTCGCTTTGCCACCAATGTGTCTTTTTAATGCCATCCATGGGGTTCTTCCGCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCg                                                                                                                                      >  2:978967/1‑150 (MQ=255)
               cATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTATCCg                                                                                                                                      <  2:609976/150‑1 (MQ=255)
                 ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGac                                                                                                                                    <  2:1058262/150‑1 (MQ=255)
                 ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGac                                                                                                                                    >  1:30023/1‑150 (MQ=255)
                 ttAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTATCCgtc                                                                                                                                    <  1:634296/150‑3 (MQ=255)
                      acaacaAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGATTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTGCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGAcaacaa                                                                                                                               <  2:710905/150‑1 (MQ=255)
                       caacaaGGTAACCCACCAGCAGGCCGAGAAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTGCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACaacaac                                                                                                                              >  1:540839/1‑150 (MQ=255)
                         acaaGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTg                                                                                                                            >  2:663176/1‑150 (MQ=255)
                           aaGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTTCACGCCTTATCCGACAACAACTGcc                                                                                                                          <  2:850525/150‑1 (MQ=255)
                           aaGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATATCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTGCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGcc                                                                                                                          <  1:1099198/150‑1 (MQ=255)
                            aGGTACCCCACCAGCAGGCCGAGCAGACCTCGCACTGACCATTTCAGCGCGTAGCTTTGCCCGCAATCTTTCGATTTAATGACATCCCTGGGGTTCTTCCTCATTGCAGGACGGATACGGCTTTCCCGCCATAGCCGACAAAAACTGcca                                                                                                                         >  1:640662/1‑149 (MQ=255)
                             ggTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTg                                                                                                                        >  1:1145766/1‑150 (MQ=255)
                              gTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGa                                                                                                                       >  2:179864/1‑150 (MQ=255)
                                 aCCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGc                                                                                                                    <  1:323729/150‑1 (MQ=255)
                                       cagcagGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCt                                                                                                              <  2:517100/150‑1 (MQ=255)
                                          cagGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAc                                                                                                           >  2:651326/1‑150 (MQ=255)
                                           ttgCCGAGCATACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAATGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcg                                                                                                          <  2:526513/148‑1 (MQ=255)
                                           agGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcg                                                                                                          <  1:1166100/150‑1 (MQ=255)
                                            ggCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgc                                                                                                         >  2:77202/1‑150 (MQ=255)
                                             gCCGAGCAGACCTAGCACTGACCATTTCAGCGCGGCGCTTTGCCACCAAAGATTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCCGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgcg                                                                                                        <  1:910458/150‑1 (MQ=255)
                                               cGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCCTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTc                                                                                                      <  1:940833/150‑1 (MQ=255)
                                               cGAGCAGACCTAGCACTGACCATTTCAGCGCGGCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATCAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTc                                                                                                      <  1:95195/150‑1 (MQ=255)
                                                  gCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTa                                                                                                   <  1:693723/150‑1 (MQ=255)
                                                         tcgCACTCACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGCATAAGGCTTTCTCGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGcc                                                                                            <  2:329042/148‑1 (MQ=255)
                                                         tAGCACTGAGCATTTCAGCGCGTCGCTTTGCGACCAATGTTTGTTTTTAATGTCATCCATGGGGTTGTTCCTCTTTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGcc                                                                                            <  1:860378/150‑1 (MQ=38)
                                                         tAGCACTGACGCTTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTATAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGcc                                                                                            <  2:89845/150‑1 (MQ=255)
                                                         tAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGcc                                                                                            <  2:1039065/150‑1 (MQ=255)
                                                         tAGCACTGACCATTTCAGCGCGGCGCTTTGCCACCAATGTTTCTTTTTCATGACTTCCCTGGGGTGCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGcc                                                                                            <  1:33388/150‑1 (MQ=38)
                                                            cACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTAc                                                                                         >  1:502394/1‑150 (MQ=255)
                                                             aCTGACCATTTCAGCGCGTAGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGTGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACa                                                                                        <  1:903184/150‑1 (MQ=255)
                                                                gACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACATAc                                                                                     >  2:1032615/1‑150 (MQ=255)
                                                                gACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATAc                                                                                     <  2:771569/150‑1 (MQ=255)
                                                                   cATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGtt                                                                                  >  2:717597/1‑150 (MQ=255)
                                                                      ttCAGCGCGTCGCTTTGCCACTAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATATGGATTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCg                                                                               <  2:494671/150‑1 (MQ=255)
                                                                       tCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCgg                                                                              >  1:1262186/1‑150 (MQ=255)
                                                                       tCAGCGCGGCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCgg                                                                              <  2:1031812/150‑1 (MQ=255)
                                                                        aagagcgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGt                                                                             <  2:811570/146‑1 (MQ=255)
                                                                           cgcgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtttt                                                                          <  2:174586/150‑1 (MQ=255)
                                                                             cgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGt                                                                        <  1:7317/150‑1 (MQ=255)
                                                                             cgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGt                                                                        >  2:547620/1‑150 (MQ=255)
                                                                             cgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTGCTTCCTCATTCCTGTACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGt                                                                        <  1:231129/150‑1 (MQ=255)
                                                                              gTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTa                                                                       <  2:156216/150‑1 (MQ=255)
                                                                              gTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCATTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTa                                                                       <  1:820623/150‑1 (MQ=255)
                                                                               tCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACATCTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAg                                                                      <  1:28885/150‑1 (MQ=255)
                                                                               tCGCTTTGCCACAATTGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACTCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAg                                                                      <  1:1299301/150‑1 (MQ=255)
                                                                                 gCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGc                                                                    <  1:556566/150‑1 (MQ=255)
                                                                                       ccaccaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGCCGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACa                                                              >  2:312654/1‑150 (MQ=255)
                                                                                        caccaATGTTTCTTTTTAATGACATCCATTGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTAACGCCTTATCCGACAACCACTGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAACATACGTTACGGATTTGTAGGCCGGACaa                                                             >  1:1033262/1‑150 (MQ=255)
                                                                                           caATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGTGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGc                                                          <  1:837834/150‑1 (MQ=255)
                                                                                           caATGTTTCTTTTTAATGACATCCATGGGGGTCGTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAGCAACTGCCTGATGTGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGc                                                          <  1:567748/150‑1 (MQ=255)
                                                                                               gTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTc                                                      <  1:17318/150‑1 (MQ=255)
                                                                                               gTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTc                                                      >  2:733428/1‑150 (MQ=255)
                                                                                                   gtttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgc                                                  <  1:1298032/149‑1 (MQ=255)
                                                                                                     ttttAATGACATCCATTGGGAGCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTAATCCGACAACAACTGCCTGATGCGACGCTGACGCGGCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgccg                                                <  2:1128712/150‑1 (MQ=38)
                                                                                                     ttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgccg                                                <  2:1235801/150‑1 (MQ=255)
                                                                                                      tttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgccgc                                               >  1:450866/1‑150 (MQ=255)
                                                                                                             ccATCCATGGGGTGCTTCCTCATTCCAGGACGTATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGc                                        <  1:690164/149‑1 (MQ=255)
                                                                                                              cATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACCAGGCGTACACGCCGCATCCGGCa                                       >  2:1179329/1‑150 (MQ=255)
                                                                                                              cATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCa                                       >  2:1184758/1‑150 (MQ=255)
                                                                                                              cATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCa                                       >  1:7187/1‑150 (MQ=255)
                                                                                                                tCCATTGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGATGCGGCGTGAACGCCTTGTCCGGccta                                     <  1:658139/150‑4 (MQ=25)
                                                                                                                  cATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGATGCGGCGTGAACGCCTTGTCCGGcctaca                                   <  1:165688/150‑6 (MQ=37)
                                                                                                                  cATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCAttta                                   >  2:1182749/1‑149 (MQ=255)
                                                                                                                     ggggTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTcaca                                <  2:1269293/150‑1 (MQ=255)
                                                                                                                       ggTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCCCGCCGCATCCGGCATTTCACAGc                              >  2:155469/1‑150 (MQ=255)
                                                                                                                       ggTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGc                              >  1:771278/1‑150 (MQ=255)
                                                                                                                        gTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCa                             <  2:517956/150‑1 (MQ=255)
                                                                                                                         ttcttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGAATTTCACAGCAt                            >  1:1233563/1‑150 (MQ=255)
                                                                                                                          tcttcCTCATTCCAGGACGGATAAGGCTTTCATGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGCCAAGGCGTTCACGCCGCATCCGGCATTTCACAGCAtt                           >  2:705714/1‑150 (MQ=255)
                                                                                                                            ttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCAGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTGACAGCATTAc                         <  2:1055760/150‑1 (MQ=255)
                                                                                                                             tcCTCATTCCAGGATGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACt                        <  1:367629/150‑1 (MQ=255)
                                                                                                                                 cATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCCCGCCGCATCCGGCATTTCACAGCATTACTTGGt                    >  2:552503/1‑150 (MQ=255)
                                                                                                                                  aTTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTg                   <  1:520688/150‑1 (MQ=255)
                                                                                                                                      cAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATAc               >  2:46691/1‑150 (MQ=255)
                                                                                                                                        ggACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCATACATACGGTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCTGGCATTTCACAGCATTACTTGGTGATACGa             >  2:211676/1‑150 (MQ=255)
                                                                                                                                         gACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAg            >  2:295168/1‑150 (MQ=255)
                                                                                                                                            ggATAAGGCGTGAAAGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGACATTTCACAGCATTACTTGGTGATACGAGTCt         >  2:1125430/1‑150 (MQ=38)
                                                                                                                                            ggATAAGGCGTGAAAGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGACATTTCACAGCATTACTTGGTGATACGAGTCt         >  2:1115409/1‑150 (MQ=38)
                                                                                                                                             gATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGCTGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTg        <  2:310893/150‑1 (MQ=255)
                                                                                                                                                  ggCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCCCAGCATTACTTGGTGATACGAGTCTGCGCGt   >  2:745701/1‑150 (MQ=255)
                                                                                                                                                  ggCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGt   >  1:1161590/1‑150 (MQ=255)
                                                                                                                                                  ggCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGt   >  2:785484/1‑150 (MQ=255)
                                                                                                                                                  ggCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGt   >  2:881566/1‑150 (MQ=255)
                                                                                                                                                   gCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTc  >  2:588863/1‑150 (MQ=255)
                                                                                                                                                   gCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCAATACTTGGTGATACGAGTCTGCGCGTc  >  1:1275721/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTCCCCTTGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTC  >  NC_000913/4244959‑4245255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: