Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 500,194 C→A 7.1% Q24K (CAA→AAA)  gsk → inosine/guanosine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913500,1940CA 7.1% 238.1 / 4.1 84Q24K (CAA→AAA) gskinosine/guanosine kinase
Reads supporting (aligned to +/- strand):  ref base C (44/34);  new base A (3/3);  total (47/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

TTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGC  >  NC_000913/500045‑500342
                                                                                                                                                     |                                                                                                                                                    
ttGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGc                                                                                                                                                      <  1:677295/150‑1 (MQ=255)
    aaCTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAAtt                                                                                                                                                  <  1:5782/150‑1 (MQ=255)
         gCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGAAATTccagc                                                                                                                                             >  1:851285/1‑150 (MQ=255)
         gCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACACTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGAAATTccagc                                                                                                                                             >  1:816380/1‑150 (MQ=255)
         gCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACACTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGAAATTccagc                                                                                                                                             >  1:812503/1‑150 (MQ=255)
            gCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTccagccag                                                                                                                                          >  2:1174491/1‑150 (MQ=255)
              gTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTGCAGCAATTCCAGCCAGaa                                                                                                                                        >  2:165840/1‑150 (MQ=255)
                ctcGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTTCTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGaaaa                                                                                                                                      >  1:131801/1‑150 (MQ=255)
                ctcGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGaaaa                                                                                                                                      <  1:183063/150‑1 (MQ=255)
                  cgcgcgCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACg                                                                                                                                    <  2:263716/150‑1 (MQ=255)
                      cgcTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAAc                                                                                                                                >  1:878733/1‑150 (MQ=255)
                      cgcTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAAc                                                                                                                                <  1:273878/150‑1 (MQ=255)
                        cTCCCGTCAGTTTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCa                                                                                                                              <  1:479801/150‑1 (MQ=255)
                                 taTTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCt                                                                                                                     <  2:320816/149‑1 (MQ=255)
                                 gATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCt                                                                                                                     <  1:958986/150‑1 (MQ=255)
                                   ttGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTgg                                                                                                                   <  2:1295096/150‑1 (MQ=255)
                                       gtTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAg                                                                                                               <  2:1145826/150‑1 (MQ=255)
                                       gtTAACATTCGCCGCTCAGTTAACCACCCATAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCCGCGCTGCCTGGGTAg                                                                                                               >  1:1126200/1‑150 (MQ=255)
                                         tAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAACCGAAACCAGCGCTGCCTGGGTAGTg                                                                                                             >  2:822642/1‑150 (MQ=255)
                                          aaCATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTACACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTgg                                                                                                            <  2:102332/150‑1 (MQ=255)
                                          aaCATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTgg                                                                                                            >  1:1095674/1‑150 (MQ=255)
                                           aCATTCGCCGCTCAGTTAACCACCCTTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTggg                                                                                                           <  2:682612/150‑1 (MQ=255)
                                           aCATTCGCCGCTCAGTTAACCACCCGTAAAAACCACCATGAAATTTCCCGGTAAGCGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTggg                                                                                                           >  1:800222/1‑150 (MQ=255)
                                           aCATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTggg                                                                                                           <  1:724430/150‑1 (MQ=255)
                                             aTTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTa                                                                                                         <  2:1276286/150‑1 (MQ=255)
                                               tCGCAGCTCAGTTAACCACCCGTAAAAACAACCATGATATTTCCAGGTAAACGTAATTCCAAACATTACTTCCCCGTAAACGCACGCGTTCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGTTATc                                                                                                       <  1:812097/150‑1 (MQ=255)
                                                   cgcTCAGTTAACCACCCGTAAAAACAACCATGAACTTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATc                                                                                                   <  1:528521/150‑1 (MQ=255)
                                                   cgcTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCTGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATc                                                                                                   >  2:1020288/1‑150 (MQ=255)
                                                   cgcTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATc                                                                                                   >  2:595675/1‑150 (MQ=255)
                                                    gcTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCCGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCa                                                                                                  >  2:1119153/1‑150 (MQ=255)
                                                     cTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCaa                                                                                                 <  2:521472/150‑1 (MQ=255)
                                                       cAGTTAACCCCCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCCGCGCTGCCTGGGTAGTGGGTATCGATCAAAc                                                                                               >  1:1114095/1‑150 (MQ=255)
                                                           tAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTg                                                                                           <  2:859375/150‑1 (MQ=255)
                                                            aaCCACCCGTATAAACAACCATGAAATTTCGCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCGTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTgg                                                                                          <  2:838066/150‑1 (MQ=255)
                                                                accCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGa                                                                                      <  1:883742/150‑1 (MQ=255)
                                                                 cccGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCGAGATAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCACTCGCTGGTCGat                                                                                     >  2:189584/1‑150 (MQ=255)
                                                                  ccGTAAAAACAACCATGAAATTTCCCGGTATACGTAAATCATAACATTACTTCCCCGGAAACGCACGCGATCCGCTGCTGCAGCAAGTCCAGACAGAAAACGAAACCAGAGCTGCCGGCGTAGTGGGTATCGATCACACGCTGGTCGata                                                                                    >  1:196482/1‑150 (MQ=255)
                                                                   cGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGatat                                                                                   >  1:778339/1‑150 (MQ=255)
                                                                   cGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGatat                                                                                   >  2:1132592/1‑150 (MQ=255)
                                                                   cGTAAAAACAACCATGAAATTTCCAGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCAAGAGATCCTCTGCTTCAGCAATTACAGCCAGAAAACGAAACCCGCGCTGCCTGGGTAGTGGTTATCGATCAAACGCGGGTAGatat                                                                                   >  1:227846/1‑150 (MQ=255)
                                                                    gTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATAtt                                                                                  >  2:285795/1‑150 (MQ=255)
                                                                      aaaaaCAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGa                                                                                >  2:167007/1‑150 (MQ=255)
                                                                       aaaaCAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGATGGTCGATATTGaa                                                                               >  1:970869/1‑150 (MQ=255)
                                                                        aaaCAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAg                                                                              <  1:50147/150‑1 (MQ=255)
                                                                                  gAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGga                                                                    >  1:69209/1‑150 (MQ=255)
                                                                                      tttCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCCGCCAGAAAACGAAACCCGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGgatgat                                                                >  2:710742/1‑150 (MQ=255)
                                                                                         cccGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAAc                                                                                               >  2:621344/1‑116 (MQ=255)
                                                                                         cccGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAAc                                                                                               <  1:679668/116‑1 (MQ=255)
                                                                                         cccGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAAc                                                                                               <  1:621344/116‑1 (MQ=255)
                                                                                         cccGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAAc                                                                                               >  2:679668/1‑116 (MQ=255)
                                                                                          ccGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAAt                                                            >  1:106789/1‑150 (MQ=255)
                                                                                          ccGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCATGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAAt                                                            >  1:1108264/1‑150 (MQ=255)
                                                                                           cGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAAtt                                                           <  1:959417/150‑1 (MQ=255)
                                                                                                aaagTAAATCACAACATTACGTCCCCGAAAACGCACGCGATCCGCTGCTTCCGCAATTCCCGCCCGAAAACGAAACCAGCGCTGCATGGGGAGTGGGTATCTATCAAACGCGGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTg                                                      <  1:783926/147‑1 (MQ=255)
                                                                                                aaCGTAACTCGAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAACCGCTGGTCGATATTGTAGGGAAAGTGGATGATGAATTTATTg                                                      <  1:183968/150‑1 (MQ=255)
                                                                                                       aTCCAAACATTACTTCCCAGTAAACGCACGCGATCCGCTGCTTCAGCTATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTa                                               <  1:1045806/150‑1 (MQ=255)
                                                                                                          cAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATgg                                            >  2:671422/1‑150 (MQ=255)
                                                                                                              aaTTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTa                                        <  2:552561/149‑1 (MQ=255)
                                                                                                                ttACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAg                                      <  1:264073/150‑1 (MQ=255)
                                                                                                                       cccGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCTATCAACCGCTGGGCGATATTGAATCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAATCTCCggg                               >  1:522654/1‑150 (MQ=255)
                                                                                                                       cccGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCggg                               >  2:765225/1‑150 (MQ=255)
                                                                                                                         cGTAAACGCACGCGATCCGCTGCTTCAGCAATTCAAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAACCGCTGGTCGATATTGAAGCGAAAGTGGATGATGAAGTTATTGAGCGTTATGGATTTAGCGCCGGGCa                             >  1:205616/1‑150 (MQ=255)
                                                                                                                            aaaCGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCTAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCAtta                          >  2:886962/1‑149 (MQ=255)
                                                                                                                            aaaCGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTc                          >  1:1052815/1‑150 (MQ=255)
                                                                                                                            aaaCGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTc                          <  2:106789/150‑1 (MQ=255)
                                                                                                                                gcacgcGATCCGCTGCTTCAGAAATTCCAGCCAGAAAACGAAACCAGCGCTTCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTg                      <  2:816380/150‑1 (MQ=255)
                                                                                                                                gcacgcGATCCGCTGCTTCAGAAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTg                      <  2:851285/150‑1 (MQ=255)
                                                                                                                                gcacgcGATCCGCTGCTTCAGAAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTg                      <  2:812503/150‑1 (MQ=255)
                                                                                                                                 cacgcGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTgg                     >  2:392147/1‑150 (MQ=255)
                                                                                                                                 cacgcGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTgg                     >  2:278549/1‑150 (MQ=255)
                                                                                                                                 cacgcGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTgg                     <  1:1316262/150‑1 (MQ=255)
                                                                                                                                 cacgcGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTgg                     >  1:977985/1‑150 (MQ=255)
                                                                                                                                  acgcGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGt                    <  2:405339/150‑1 (MQ=255)
                                                                                                                                  acgcGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGt                    >  1:433143/1‑150 (MQ=255)
                                                                                                                                     cgATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGACACCAGCGCTGCCTGGGTAGTGGGTATCGATCCAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGAt                 <  2:1052311/150‑1 (MQ=255)
                                                                                                                                            ctgctTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGgat          >  2:1168960/1‑150 (MQ=255)
                                                                                                                                              gctTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGgatga        >  1:1069780/1‑150 (MQ=255)
                                                                                                                                               ctTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGgatgat       <  2:1095674/150‑1 (MQ=255)
                                                                                                                                                ttCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGatgatg      >  2:1196108/1‑150 (MQ=255)
                                                                                                                                                 tCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGt     >  2:531963/1‑150 (MQ=255)
                                                                                                                                                   aGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAg   >  2:1043914/1‑150 (MQ=255)
                                                                                                                                                   aGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAg   >  1:112596/1‑150 (MQ=255)
                                                                                                                                                    gCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGc  >  2:1185143/1‑150 (MQ=255)
                                                                                                                                                    gCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGc  <  2:878733/150‑1 (MQ=255)
                                                                                                                                                    gCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACAGGTGATTGATGATGATGTAGc  >  1:330400/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAGCGTTATGGATTAAGCGCCGGGCATTCACTGGTGATTGAGGATGATGTAGC  >  NC_000913/500045‑500342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑