Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,261,349 G→A 6.6% intergenic (+43/‑320) yjbM → / → dusA uncharacterized protein/tRNA‑dihydrouridine synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,261,3490GA 6.6% 211.0 / 5.1 79intergenic (+43/‑320)yjbM/dusAuncharacterized protein/tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand):  ref base G (38/33);  new base A (2/3);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

GCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATT  >  NC_000913/4261203‑4261497
                                                                                                                                                  |                                                                                                                                                    
gCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGTGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATATTATTGAATGCATTATATAAAAGGAcc                                                                                                                                                   >  1:843990/1‑150 (MQ=255)
 cTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTTAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGAccc                                                                                                                                                  >  1:121277/1‑150 (MQ=255)
 cTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGAccc                                                                                                                                                  <  1:57468/150‑1 (MQ=255)
     gcgAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAata                                                                                                                                              >  2:1053318/1‑150 (MQ=255)
     gcgAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAata                                                                                                                                              <  2:1001176/150‑1 (MQ=255)
      cgAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCAAatat                                                                                                                                             >  2:325101/1‑150 (MQ=255)
         aGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAAtattta                                                                                                                                          <  2:967165/150‑1 (MQ=255)
          gTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAAtatttat                                                                                                                                         >  1:919331/1‑150 (MQ=255)
                tGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATAAATATTGGGtc                                                                                                                                   <  1:564133/150‑2 (MQ=255)
                  aCCCAACCTGAAAGGAAGAGATTAAAAGACTTTCAGACACTTCTAAATTAATTTATGATGTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCt                                                                                                                                 >  2:900930/1‑150 (MQ=255)
                  aCCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCt                                                                                                                                 >  1:491424/1‑150 (MQ=255)
                    ccAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGAATTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGAACCCAATAAATATTGGGTCCttt                                                                                                                               <  1:1121800/150‑1 (MQ=255)
                    caaaCCTAAAATGAAGATATTAAAATACTTAAAGACACTTCTAAATTAATTTATGATTTTTTCGGGGTTAAATAGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGAACCCAATAAATATTGGGTCCttt                                                                                                                               <  1:908666/148‑1 (MQ=255)
                     cAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCtttt                                                                                                                              >  1:220476/1‑150 (MQ=255)
                     cAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGAACCCAATAAATATTGGGTCCtttt                                                                                                                              <  1:1230480/150‑1 (MQ=255)
                        ccTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATTGAAGGCATTATATAAAAGGACCCAATATTTATTTGTTTCttttata                                                                                                                           >  1:1216886/1‑147 (MQ=255)
                        ccTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGTTTCtttttta                                                                                                                           >  1:1140543/1‑149 (MQ=255)
                              aGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATc                                                                                                                     <  2:1311814/150‑1 (MQ=255)
                               ggAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCa                                                                                                                    >  2:719062/1‑150 (MQ=255)
                                gAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCaa                                                                                                                   >  1:819745/1‑150 (MQ=255)
                                 aaGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAAt                                                                                                                  >  2:832254/1‑150 (MQ=255)
                                       ttAAAATACTTACAGAAACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTAATTGGGTTCTTTTTTCTCTATCAATACTAtt                                                                                                            <  1:157564/150‑1 (MQ=255)
                                              aCTTACAGACACTTTTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAggg                                                                                                     >  2:847687/1‑150 (MQ=255)
                                              aCTTACAGACACTTCTAAATTAATTTATGATTTGTTCGTGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAggg                                                                                                     <  1:277569/150‑1 (MQ=255)
                                                 tACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGgaga                                                                                                  >  1:979310/1‑150 (MQ=255)
                                                   cAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATAATATTAGCAGGGAGata                                                                                                >  1:1024142/1‑150 (MQ=255)
                                                   aaGACACTTCTAAATTAAATTATGATTTGTTCGCGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTAAGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGata                                                                                                <  1:207477/149‑1 (MQ=255)
                                                    aGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGatat                                                                                               >  2:83400/1‑150 (MQ=255)
                                                     gACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGatata                                                                                              >  1:189985/1‑150 (MQ=255)
                                                     gACAATTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCTATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTATATAAATACTTATAGAAGGGAGatata                                                                                              >  2:461648/1‑150 (MQ=255)
                                                        acTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCa                                                                                           <  2:656606/150‑1 (MQ=255)
                                                              aaaTTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCagag                                                                                     >  1:609897/1‑150 (MQ=255)
                                                                attaattTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGtt                                                                                   <  1:1011800/150‑1 (MQ=255)
                                                                    attTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGAATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAt                                                                               >  2:585496/1‑150 (MQ=255)
                                                                     tttATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAtg                                                                              <  1:160090/150‑1 (MQ=255)
                                                                      ttATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAtgt                                                                             >  2:860292/1‑150 (MQ=255)
                                                                       tATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAtgtg                                                                            >  1:1235590/1‑150 (MQ=255)
                                                                        aTGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAtgtgt                                                                           >  2:441654/1‑150 (MQ=255)
                                                                         tGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAtgtgtg                                                                          >  1:612334/1‑150 (MQ=255)
                                                                          gATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGa                                                                         >  2:737408/1‑150 (MQ=255)
                                                                          gATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGa                                                                         >  2:1078136/1‑150 (MQ=255)
                                                                             ttGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGAttt                                                                      <  2:408933/150‑1 (MQ=255)
                                                                                   ggggGTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTtttatt                                                                <  1:1067618/150‑1 (MQ=255)
                                                                                         aaaTCGGAGATATAATATGTGAACTTCTTGGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGTTATATCACCAGAGTTTAATGTGTGATTTTTTATTAAtcgt                                                          <  2:108294/150‑1 (MQ=255)
                                                                                          aaTCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTAtcgtc                                                         <  1:973941/150‑1 (MQ=255)
                                                                                           aTCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTAtcgtcg                                                        <  2:854830/150‑1 (MQ=255)
                                                                                             cGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGATATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGaa                                                      >  1:63607/1‑150 (MQ=255)
                                                                                             cGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGaa                                                      >  2:908631/1‑150 (MQ=255)
                                                                                             cGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGaa                                                      >  1:762954/1‑150 (MQ=255)
                                                                                              ggAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGTCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAAc                                                     <  2:363118/150‑1 (MQ=255)
                                                                                              ggAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGGTCTTTTTTCGCTATCAATACTAGTAGCAGGGAGATATATCACCAGAGGTTTATGTGTGATATTTTATTTATCGTCGAAc                                                     >  1:834089/1‑150 (MQ=255)
                                                                                               gagaAATAATATGTGAAGTTCTTCGATAGTATGGAAGGAATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAAcc                                                    <  1:666865/150‑1 (MQ=255)
                                                                                                agaAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCt                                                   >  1:833776/1‑150 (MQ=255)
                                                                                                agaAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCt                                                   >  2:433349/1‑150 (MQ=255)
                                                                                                    ataataTGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGAt                                               <  1:308301/150‑1 (MQ=255)
                                                                                                         atGTTAAGATCTTCGATAGTATCGTAGGCATTATATAAAAGGACCCAATATGAATTTGGTTCTTTTTTCTCTATCAATACTATAAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGttt                                          <  2:236338/150‑1 (MQ=255)
                                                                                                          tgtgAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTa                                         <  2:1047750/150‑1 (MQ=255)
                                                                                                            tgAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAAAACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTAta                                       >  1:1110192/1‑149 (MQ=255)
                                                                                                             taaGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCa                                      <  1:1116826/149‑1 (MQ=255)
                                                                                                                 ttcttcGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTgg                                  >  1:274122/1‑150 (MQ=255)
                                                                                                                  tcttgGATAGTATGGAAGGATTTATATAAAAGCACCAAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCGGGTTTGTTTATCATTGGc                                 <  2:257459/150‑1 (MQ=255)
                                                                                                                  tcttcGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGc                                 <  2:382320/150‑1 (MQ=255)
                                                                                                                   tttcGATAGTATGGAATGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGcc                                <  1:320164/149‑1 (MQ=255)
                                                                                                                   cttcGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGcc                                <  2:291710/150‑1 (MQ=255)
                                                                                                                        aTAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTaaa                           >  1:490715/1‑149 (MQ=255)
                                                                                                                         tAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACa                          <  2:491424/150‑1 (MQ=255)
                                                                                                                            tATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAg                       <  1:1281807/150‑1 (MQ=255)
                                                                                                                            tATGGAAGGCATTATATAAAAGAACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAg                       >  2:757225/1‑150 (MQ=255)
                                                                                                                                 aatgCATTATATAAAAGGAACCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAAc                  <  2:1263000/147‑1 (MQ=255)
                                                                                                                                  aGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACg                 >  2:1028284/1‑150 (MQ=255)
                                                                                                                                  aGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACg                 <  2:220476/150‑1 (MQ=255)
                                                                                                                                    gCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACggg               >  1:1143480/1‑149 (MQ=255)
                                                                                                                                     cATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCt              <  1:83400/150‑1 (MQ=255)
                                                                                                                                         agaaaaaaGAACCCAATAAATATTGGGTCCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCtaata          >  2:608474/5‑150 (MQ=255)
                                                                                                                                           ataAAAGTACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAg        <  2:49266/150‑1 (MQ=255)
                                                                                                                                           ataAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAg        <  2:612334/150‑1 (MQ=255)
                                                                                                                                             aaaaGTTCCCAATATTTATTGGGTTCTTTTTTCTATATCAATACTATTAGCCGGGATATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTCGCCTTAACAAAGTTAACGGCTAATAAGat      <  1:69869/150‑1 (MQ=255)
                                                                                                                                              aaaGGACCCAATATTTATTGGGTTCTTTTTTCTATATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTTATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGatt     <  2:766921/150‑1 (MQ=255)
                                                                                                                                               aaGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGatta    <  2:919331/150‑1 (MQ=255)
                                                                                                                                                 ggACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGattatt  >  1:522599/1‑150 (MQ=255)
                                                                                                                                                 ggACCCAATATTTATTGGGTTCTTTTTTATCTATCAATACTATTAGCATGGAGATAAATCACCATATTTTAATGTTTTATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGattatt  <  1:535001/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCTTCGCGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATT  >  NC_000913/4261203‑4261497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: