Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,336,979 A→C 8.4% M18R (ATG→AGG)  adiC ← arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,336,9790AC 8.4% 275.3 / 12.2 103M18R (ATG→AGG) adiCarginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base A (47/47);  new base C (6/3);  total (53/50)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 103 total aligned reads displayed.

GTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGC  >  NC_000913/4336831‑4337127
                                                                                                                                                    |                                                                                                                                                    
gTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCAt                                                                                                                                                     >  1:320507/1‑150 (MQ=255)
  cGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCa                                                                                                                                                   >  2:1011748/1‑150 (MQ=255)
   gAGGAACGACGTTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCAACAACCATCCATAAATGGCAATCCCGCCAGAAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCAGACACCATCAg                                                                                                                                                  >  1:857604/1‑150 (MQ=255)
   gAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAg                                                                                                                                                  <  1:1192153/150‑1 (MQ=255)
     tgAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCTCCAACCATCCATAAATGTCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAATACACCTGACCCCATAATATTCCCCGACACCATCAggg                                                                                                                                                <  2:1224539/149‑1 (MQ=255)
        aCGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCAGCAGGGTGa                                                                                                                                             >  1:494044/1‑150 (MQ=255)
          gACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCCTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACCCCTGACCCCATAATATTCCCCGGCACCATCCGGGTGCCg                                                                                                                                           >  2:452385/1‑150 (MQ=255)
            cATTTTTGCGTATACCATCTAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGTCACCCTTATGGTGTCggg                                                                                                                                         <  1:1232079/150‑1 (MQ=255)
            cATTTTTGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCAAAATATTCCCCGACACCATCAGGGTGACggg                                                                                                                                         <  1:1157204/150‑1 (MQ=255)
             aTTTTGTCGTATACCATCGAGCGCGCCAGTGCACCTATAATCGTCACCACCAATGCATAAATTGCACTCCCGCGAGTAGAGGCCAGGTATGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGTCACCCTGATGGTGTCgggg                                                                                                                                        <  2:206785/150‑1 (MQ=255)
               ttacgCGTATACCAACCACTGCCCCACTTCACCGATAATCGTCAACTCCCATCCATAAATGGCAATCCCGCCAGTAGAGGCGAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATACTATTCCCCCACCCCATCAGGGTGACGGGGAt                                                                                                                                      <  2:78013/146‑1 (MQ=255)
                 tGGCGTATACCATCTAGATCCCCTGTGCCCCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTAGCAGGTAACAGAAATACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTa                                                                                                                                    <  2:1212275/150‑1 (MQ=255)
                 tGGCGTATACCATCGACAGCCCCAGTGCACCGATAATCGTCACCCACCATCCATAAATGGCAAGCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTa                                                                                                                                    <  1:761272/150‑1 (MQ=255)
                  ggCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACCCCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTaa                                                                                                                                   >  2:26035/1‑150 (MQ=255)
                  ggCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTaa                                                                                                                                   >  2:21571/1‑150 (MQ=255)
                   gCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCTCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAg                                                                                                                                  >  2:1093474/1‑150 (MQ=255)
                     gTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGcc                                                                                                                                <  1:441990/150‑1 (MQ=255)
                      tataCCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGccc                                                                                                                               <  2:441567/150‑1 (MQ=255)
                       ataCCATCGAGAGCCCCAGTGCACCGATAATCGTCACAAACCATCCATAAATTGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCa                                                                                                                              <  2:1187167/150‑1 (MQ=255)
                        taCCATCGAGCGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCATTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACCACTGACCCCATAAGATTCCCCGACACCATCAGGGTGACGGGGGTTACGccccc                                                                                                                             >  1:176808/1‑148 (MQ=255)
                        taCCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCAc                                                                                                                             <  1:429539/150‑1 (MQ=255)
                           cATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACttt                                                                                                                          >  2:425655/1‑150 (MQ=255)
                              cGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTtgtg                                                                                                                       <  2:989301/150‑1 (MQ=255)
                                agagCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGag                                                                                                                     <  1:464662/150‑1 (MQ=255)
                                agagCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGag                                                                                                                     <  1:552144/150‑1 (MQ=255)
                                agagCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGACAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGag                                                                                                                     <  1:603815/150‑1 (MQ=255)
                                  agCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagca                                                                                                                   >  1:198041/1‑150 (MQ=255)
                                    ccccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatc                                                                                                                 >  2:770668/1‑150 (MQ=255)
                                    ccccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatc                                                                                                                 <  2:1120734/150‑1 (MQ=255)
                                                gATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCACCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGa                                                                                                     >  2:184843/1‑150 (MQ=255)
                                                    aTCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATa                                                                                                 <  2:383550/150‑1 (MQ=255)
                                                    aTCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGGATGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGCAGACATa                                                                                                 <  2:1277909/150‑1 (MQ=255)
                                                      cGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCCCTTTGTGAGCATCAGCATCCGAAGACATAAt                                                                                               >  2:1265869/1‑150 (MQ=255)
                                                      cGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAt                                                                                               <  2:1266006/150‑1 (MQ=255)
                                                       gTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGCCCCCATAATATTCCCCGACCCCATCAGGGAGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACAAAAtt                                                                                              >  1:107558/1‑150 (MQ=255)
                                                       gTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAtt                                                                                              >  1:368882/1‑150 (MQ=255)
                                                        tCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTa                                                                                             <  1:296941/150‑1 (MQ=255)
                                                         cagcaaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaa                                                                                            <  1:652186/147‑1 (MQ=255)
                                                         cACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaa                                                                                            >  1:359798/1‑150 (MQ=255)
                                                          accaaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaaa                                                                                           >  2:260785/1‑150 (MQ=255)
                                                            caaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACt                                                                                         >  1:1051418/1‑150 (MQ=255)
                                                              accaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACGTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTcc                                                                                       <  2:198041/150‑1 (MQ=255)
                                                                catccatAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTg                                                                                     <  1:1011752/150‑1 (MQ=255)
                                                                 atccatAAATGGCCATCCCGCCAGTAGAGTCCAGGGTTGCAGGTAACAGAAACACACCTGACCCCATAATATTCCCCGACACCCTCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGc                                                                                    <  2:1110034/150‑1 (MQ=255)
                                                                  tccatAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCg                                                                                   <  1:606408/150‑1 (MQ=255)
                                                                    catAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGaa                                                                                 >  1:928339/1‑150 (MQ=255)
                                                                    catAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGAAGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGaa                                                                                 >  1:686353/1‑150 (MQ=255)
                                                                     atAAATGGCAATCCCGCCAGTAGCGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAg                                                                                >  2:610841/1‑150 (MQ=255)
                                                                        aaTGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCg                                                                             <  2:1203151/150‑1 (MQ=255)
                                                                           ggCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGc                                                                          <  1:974303/150‑1 (MQ=255)
                                                                            gCAATCCCGCCATTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCt                                                                         <  1:150846/150‑1 (MQ=255)
                                                                             cAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCtt                                                                        >  1:967381/1‑150 (MQ=255)
                                                                               aTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCg                                                                      <  2:718694/150‑1 (MQ=255)
                                                                                tCCCGCCAGTAGATGCCAGGTTTGCAGGTAACAGTAAAACACCTGACCCCATAATCTTCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTg                                                                    <  2:594389/150‑1 (MQ=255)
                                                                                tCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGt                                                                     <  2:651799/150‑1 (MQ=255)
                                                                                tCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGt                                                                     <  1:1102052/150‑1 (MQ=255)
                                                                                   cGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc                                                                  <  2:598378/150‑1 (MQ=255)
                                                                                   cGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc                                                                  <  1:876347/150‑1 (MQ=255)
                                                                                      cAGTAGAGGCCAGGTTTGCAGGTAACAGATCAACACCTGACCCCATAATATTCCCCGACACCATCAGGGGGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAAt                                                               <  1:1070465/150‑1 (MQ=255)
                                                                                       aGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATa                                                              >  2:946530/1‑150 (MQ=255)
                                                                                          agagGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGCCACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAAt                                                           >  2:865010/1‑150 (MQ=255)
                                                                                             ggCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGAGAGCTTCGTGACAATAAATTAc                                                        >  1:8443/1‑150 (MQ=255)
                                                                                              gCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACg                                                       <  2:630933/150‑1 (MQ=255)
                                                                                               ccAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGt                                                      >  1:1054820/1‑150 (MQ=255)
                                                                                               ccAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGt                                                      >  1:137544/1‑150 (MQ=255)
                                                                                                cAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtc                                                     <  2:1039932/150‑1 (MQ=255)
                                                                                                  ggTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcat                                                   >  1:1258186/1‑150 (MQ=255)
                                                                                                        cAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatcata                                             >  1:1109544/1‑150 (MQ=255)
                                                                                                          ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGCCATAAGTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACg                                           >  1:336499/1‑150 (MQ=255)
                                                                                                          ggTAACAGAAAAACACCAGACCCCATAATATTCCCCGACACCAGCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATAGGCATCCGAACACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCAAATCATACg                                           >  2:68640/1‑150 (MQ=255)
                                                                                                           gTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGc                                          <  2:857604/150‑1 (MQ=255)
                                                                                                            tAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGcc                                         <  2:975866/150‑1 (MQ=255)
                                                                                                             aaCAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCt                                        >  2:1070518/1‑150 (MQ=255)
                                                                                                              aCAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTg                                       <  2:1152764/150‑1 (MQ=255)
                                                                                                              aCAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCCTCGTGACAATAAATTACGTCATATCATACGCCTg                                       >  2:154304/1‑150 (MQ=255)
                                                                                                                aGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGTTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCa                                     <  2:318718/150‑1 (MQ=255)
                                                                                                                aGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCa                                     >  2:773966/1‑150 (MQ=255)
                                                                                                                 gAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACACCTGCAt                                    >  1:482279/1‑150 (MQ=255)
                                                                                                                  aaaaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCAtt                                   >  2:848295/1‑150 (MQ=255)
                                                                                                                  aaaaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCAtt                                   >  1:266176/1‑150 (MQ=255)
                                                                                                                      acacCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGAt                               <  2:634374/150‑1 (MQ=255)
                                                                                                                        acCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAg                             >  1:171272/1‑150 (MQ=255)
                                                                                                                              ccccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGTGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAat                       <  1:841118/150‑1 (MQ=255)
                                                                                                                               cccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatt                      >  1:452854/1‑150 (MQ=255)
                                                                                                                                      tatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCCCTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCg               >  1:50053/1‑150 (MQ=255)
                                                                                                                                      tatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACACCTGCATTCGATAGTTGAATTATTATCg               <  2:482279/150‑1 (MQ=255)
                                                                                                                                       atTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGa              >  2:1034724/1‑150 (MQ=255)
                                                                                                                                        ttCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAc             >  2:1074229/1‑150 (MQ=255)
                                                                                                                                          ccccGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATtattatatacag           >  1:75001/1‑144 (MQ=255)
                                                                                                                                           cccGTCACCCTGATGGTGTCGGGGATTTAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCAAACGCCTGCATACGATAGTTGAATTATTATCGACCgg          >  2:1014594/1‑150 (MQ=255)
                                                                                                                                           cccGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAACCTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATCCGCCTGCATTCGATAGTTGAATTATTATCGACCgg          >  1:95260/1‑150 (MQ=255)
                                                                                                                                           cccGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCgg          >  2:1010274/1‑150 (MQ=255)
                                                                                                                                           cccGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCgg          >  2:409529/1‑150 (MQ=255)
                                                                                                                                           cccGTCACCCTGATGGGGTCGAGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGAAGTCGATAGATGAATTATTATCGACCgg          >  2:579272/1‑150 (MQ=255)
                                                                                                                                           cccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCgg          >  1:802282/1‑150 (MQ=255)
                                                                                                                                            ccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCggg         <  1:304093/150‑1 (MQ=255)
                                                                                                                                             cGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGt        <  2:494044/150‑1 (MQ=255)
                                                                                                                                             cGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGt        <  1:425655/150‑1 (MQ=255)
                                                                                                                                                   cATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGc  <  2:320507/150‑1 (MQ=255)
                                                                                                                                                   cATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGc  <  1:855842/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGC  >  NC_000913/4336831‑4337127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: