Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,410,026 G→A 7.5% intergenic (+20/‑107) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,410,0260GA 7.5% 196.4 / 6.7 80intergenic (+20/‑107)rlmB/yjfI23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
Reads supporting (aligned to +/- strand):  ref base G (31/42);  new base A (3/3);  total (34/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01

TATGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTG  >  NC_000913/4409877‑4410175
                                                                                                                                                     |                                                                                                                                                      
tATGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAg                                                                                                                                                        >  1:657694/1‑150 (MQ=255)
 aTGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGc                                                                                                                                                       <  1:155796/150‑1 (MQ=255)
 aTGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCATCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGc                                                                                                                                                       <  2:793343/150‑1 (MQ=255)
    cGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCAt                                                                                                                                                    >  2:333179/1‑150 (MQ=255)
    cGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCAt                                                                                                                                                    >  1:168470/1‑150 (MQ=255)
     gTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATc                                                                                                                                                   <  1:1012867/150‑1 (MQ=255)
       cGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCa                                                                                                                                                 <  2:896991/150‑1 (MQ=255)
        gCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAg                                                                                                                                                >  1:933947/1‑150 (MQ=255)
         ccTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGa                                                                                                                                               >  1:311152/1‑150 (MQ=255)
          cTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGTCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGAt                                                                                                                                              >  1:1034064/1‑150 (MQ=255)
          cTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGAt                                                                                                                                              >  1:1109764/1‑150 (MQ=255)
            gACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGAttt                                                                                                                                            >  2:582652/1‑150 (MQ=255)
             aCTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGATGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTg                                                                                                                                           <  1:250948/150‑1 (MQ=255)
                cGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGAt                                                                                                                                        <  2:610432/150‑1 (MQ=255)
                      cATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttt                                                                                                                                 <  1:1314052/150‑1 (MQ=255)
                          gCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcat                                                                                                                             <  1:928969/150‑4 (MQ=255)
                            gATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGATTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTg                                                                                                                           <  1:364431/150‑1 (MQ=255)
                            gATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTg                                                                                                                           <  1:766363/150‑1 (MQ=255)
                            caTGAGGTGATCACCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTg                                                                                                                           <  2:1146999/149‑1 (MQ=255)
                            caTGAGGTGAGCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTg                                                                                                                           <  2:1152106/149‑1 (MQ=255)
                             aTGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGt                                                                                                                          >  1:311026/1‑150 (MQ=255)
                              tGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTc                                                                                                                         <  2:16453/150‑1 (MQ=255)
                               gAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCt                                                                                                                        >  1:251988/1‑150 (MQ=255)
                                 tttGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCtat                                                                                                                      <  1:1094319/149‑1 (MQ=255)
                                   tGAGCAGCATCCCGATGGCTGGAAGCGTTTCATCCCTGAACGTTGCGGTGGCGACCGGAATTTGCTTATTTGAAGCGGGGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAg                                                                                                                    <  1:866212/150‑1 (MQ=255)
                                       cAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTgg                                                                                                                <  1:1208214/150‑1 (MQ=255)
                                           aTCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAg                                                                                                            <  2:1296130/150‑1 (MQ=255)
                                            tCCCGCTGGCTGGAAGCGTTTCTTCCCGGAACGGTTCGGTTGCGACCGGAATTTGCATATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCCGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGa                                                                                                           <  2:1082145/150‑1 (MQ=255)
                                             cccGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACAGGAATTTGCTTATATCAAGCTGTGCGCCAGCACAGCTAATTTATCAGATTTATGAAAAGCCATCCAGATTTGGATGGTGTTTTTTTGTCTATAGCTGGTAAGAt                                                                                                          >  1:197574/1‑150 (MQ=255)
                                               cGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATaa                                                                                                        >  1:723853/1‑150 (MQ=255)
                                                gATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAAt                                                                                                       >  1:1268888/1‑150 (MQ=255)
                                                  tGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                                                     <  2:99736/150‑1 (MQ=255)
                                                    gCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACg                                                                                                   <  1:511109/150‑1 (MQ=255)
                                                    gCTGGAAGCGTTGCTTGCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCATAATTTTGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACg                                                                                                   <  1:1230933/150‑1 (MQ=255)
                                                     cTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACAGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGt                                                                                                  <  1:185625/150‑1 (MQ=255)
                                                           gCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCa                                                                                            >  2:1021152/1‑150 (MQ=255)
                                                             gTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGaaaa                                                                                          >  2:764593/1‑150 (MQ=255)
                                                                  ttcCCTGAACGTTTCGGTTGCGACCGTAATTTGCTTATGTGACGCGGTGCGCCAGCGCAGCTAATTTCTCATAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTc                                                                                     <  1:675904/150‑1 (MQ=255)
                                                                     ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtt                                                                                  >  1:113645/1‑150 (MQ=255)
                                                                     ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGCTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCAATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtt                                                                                  >  2:139302/1‑150 (MQ=255)
                                                                      cTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGTATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt                                                                                 <  1:667203/150‑1 (MQ=255)
                                                                            gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTaa                                                                           >  1:163444/1‑150 (MQ=255)
                                                                              ttCGGTTGCTACCGGAAATTGCTTATTTGAAGCGGTGCGCCAGCGCATCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGAAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                                                         <  1:978439/150‑1 (MQ=255)
                                                                               tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATTAAAAGCCATCCACTTTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAatat                                                                        <  2:215864/150‑1 (MQ=255)
                                                                                 ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAttt                                                                      >  2:722162/1‑150 (MQ=255)
                                                                                      cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                                                                 >  2:837753/1‑150 (MQ=255)
                                                                                       gACCGGAATTTGCTTATTTGTAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACtt                                                                <  2:849125/150‑1 (MQ=255)
                                                                                        aCCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTa                                                               >  1:514573/1‑150 (MQ=255)
                                                                                        aCCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTa                                                               <  2:1215104/150‑1 (MQ=255)
                                                                                         ccGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTTAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTaa                                                              <  2:251988/150‑1 (MQ=255)
                                                                                           ggAATTTGCTTATTTGCAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATGGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                                                            <  2:772940/150‑1 (MQ=255)
                                                                                            gAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                                                           >  2:855257/1‑150 (MQ=255)
                                                                                             aaTTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCta                                                          <  2:1157935/150‑1 (MQ=255)
                                                                                             aaTTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAAATTAAtata                                                          >  1:278675/1‑150 (MQ=255)
                                                                                                    ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATTGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTTTTAATATTTAAACTTAATCTATATGGat                                                   >  1:568917/1‑149 (MQ=255)
                                                                                                    ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaa                                                   <  1:722162/150‑1 (MQ=255)
                                                                                                        ttGAAGCGGTGCGCCAGCGGAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATTGTTTTTTTTTGTCTATAGCTTGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataat                                               <  2:588107/150‑1 (MQ=255)
                                                                                                        ttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataat                                               >  1:454520/1‑150 (MQ=255)
                                                                                                        ttGAAGCGGAGCGCCAGCGCAGCTAATTTCTCAGAATTATGTAAAGACATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataat                                               <  1:567609/150‑1 (MQ=255)
                                                                                                          gAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat                                             >  2:267069/1‑150 (MQ=255)
                                                                                                              cGGTGCGACAGAGAAGCTAATTTCTGAGAATTATTAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatatta                                         <  2:1012281/150‑3 (MQ=255)
                                                                                                               ggTGCGCCAGCGCAGCTAATTTCTCAGAATTATTAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGACAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAc                                        <  2:153435/150‑1 (MQ=255)
                                                                                                                 tGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACAt                                      <  2:284243/150‑1 (MQ=255)
                                                                                                                   cgcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATgg                                    <  2:454520/150‑1 (MQ=255)
                                                                                                                      cAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTAAATCTATATGGAATAAAATATGACATGGAAt                                 >  2:820932/1‑150 (MQ=255)
                                                                                                                         cgcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCg                              <  1:492858/150‑1 (MQ=255)
                                                                                                                          gcAGCTAATTTCTCATAATTATGAAAAGCCATCCAGATTTGTATGGTTTATTTTAGACAATAGCTTTTAAGATAATTACGTATTGCAAATAATCATTTTATTAATAATCAAACTTCAGCTATATGGAATAATATATGACATGGAATCCgc                             <  1:187251/150‑2 (MQ=255)
                                                                                                                             tcTAATTTCTAAGAATTATGAAAATCCATCCAGATTTGGATTGTTTTTTTTTGTCTATAGCTGGTTATATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTgg                          <  1:1208258/149‑1 (MQ=255)
                                                                                                                             gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTgg                          <  1:333179/150‑1 (MQ=255)
                                                                                                                             gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTgg                          <  2:260374/150‑1 (MQ=255)
                                                                                                                             gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTgg                          >  2:1111686/1‑150 (MQ=255)
                                                                                                                             gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTgg                          <  1:259131/150‑1 (MQ=255)
                                                                                                                              cTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGgcg                        >  1:449484/1‑150 (MQ=255)
                                                                                                                                         gatttATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCGGGTAAGATAATTACGTATTGCAAATATTCATTTTATTTATTTTTAAACTTAATCTATATGGATTAATATATGACATGGAATCCGTTGGCGCTAGCGActg              >  1:65587/4‑148 (MQ=255)
                                                                                                                                         gAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACgg              >  2:688931/1‑150 (MQ=255)
                                                                                                                                            caaaaaaaaACCATCCAAATCTGGATGGCTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGgcg            >  2:635865/6‑150 (MQ=255)
                                                                                                                                            caaaaaaaaACCATCCAAATCTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGgcgc           >  2:699699/6‑150 (MQ=255)
                                                                                                                                            caaaaaaaaACCATCCAAATCTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGgcgc           >  2:1291862/6‑150 (MQ=255)
                                                                                                                                               tGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTTGTGTATAGCTGGTAAGATAATTACGTATTGCAAATATTAATTTTATTAATATTTAAACTTATTCTTTATGGAATAAAAAATGACAAGGAATCCGTTGGCGCTAGCGACGGCGCTg         >  1:507297/1‑150 (MQ=255)
                                                                                                                                                taaaaGCCATCCAGATTTGGATGGTTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGc        <  2:311152/149‑1 (MQ=255)
                                                                                                                                                   aaGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAAc    <  2:1169267/150‑1 (MQ=255)
                                                                                                                                                     gCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTg  >  2:1228204/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TATGCGTCGCCTGACTCGTGAACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTG  >  NC_000913/4409877‑4410175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: