Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,519,311 A→G 8.6% intergenic (+87/+27) insB1 → / ← yjhU IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,519,3110AG 8.6% 258.1 / 13.6 94intergenic (+87/+27)insB1/yjhUIS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element
Reads supporting (aligned to +/- strand):  ref base A (39/46);  new base G (5/3);  total (44/50)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.29e-01

AAATCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAG  >  NC_000913/4519162‑4519456
                                                                                                                                                     |                                                                                                                                                 
aaaTCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCata                                                                                                                                                   >  2:1016816/1‑150 (MQ=255)
aaaTCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCata                                                                                                                                                   >  2:345578/1‑150 (MQ=255)
     ggTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTAc                                                                                                                                              >  1:1236593/1‑150 (MQ=255)
      gTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACa                                                                                                                                             >  1:1265219/1‑150 (MQ=255)
         gAGTTGCATGAAAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATgg                                                                                                                                          <  2:940126/150‑1 (MQ=255)
           gTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATgggg                                                                                                                                        >  1:1072870/1‑150 (MQ=255)
            ttGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGc                                                                                                                                       <  1:547369/150‑1 (MQ=255)
            ttGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGc                                                                                                                                       <  2:277724/150‑1 (MQ=255)
               caggACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATATCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATAAATAAAATCCTGCCCCATGTAGATATGGGGCAgg                                                                                                                                    <  2:746888/147‑2 (MQ=37)
               cATGACTATGTCTTCTGGCATTATCTCAAAATAAAACTCTATCAGACAGTTTGAGTAATTAACGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATTAAATCCTGCCCCATGTAGAGATGGGGCAgg                                                                                                                                    <  2:641795/150‑2 (MQ=255)
               cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATGTAGATATGGGGCAgg                                                                                                                                    <  1:877917/150‑2 (MQ=255)
               cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgg                                                                                                                                    <  2:949686/150‑2 (MQ=255)
               cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGGATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTTCCCCATATCTACATGGGGCAgt                                                                                                                                    <  2:939118/150‑1 (MQ=255)
               aaTGAGAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTTTTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgt                                                                                                                                    <  1:108588/149‑1 (MQ=255)
                  gACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTGCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgt                                                                                                                                 >  2:923849/1‑150 (MQ=255)
                    cAAGGTCATGGGGCATTATCTCAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTc                                                                                                                               <  2:644880/150‑1 (MQ=255)
                    cAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTc                                                                                                                               <  2:946547/150‑1 (MQ=255)
                    cAAGGACATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGCAGTCATTACCGCCCATGAATATTTCATACATAGTGGGTATTGTTCTTATTATCGCAGCTAATCAATAAAATCCTGACCCATATCTACATGGGGAAgttgtta                                                                                                                               >  1:847422/1‑149 (MQ=37)
                       ggTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCAtt                                                                                                                            >  2:1073756/1‑150 (MQ=255)
                         gcATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCt                                                                                                                          <  2:1249504/149‑1 (MQ=255)
                            tCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                       >  2:713769/1‑150 (MQ=255)
                            acGGGCATTATCTGAACATAAACCCCTATCAGTAACTTTGAGTCATTACCGACCATTTTTATTTCAAACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                       <  2:1218283/149‑1 (MQ=39)
                              gggCATTATCTGAACATATAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTTTGGGTTTTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAg                                                                                                                     <  2:798545/150‑1 (MQ=255)
                                gCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAgtg                                                                                                                   <  2:1007066/150‑1 (MQ=255)
                                gCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAgtg                                                                                                                   >  1:614972/1‑150 (MQ=255)
                                 cATTATCTGAACATAAAACACTATCTTTAAGTTGGAGTCATTACCGTCCATTTTTATTTCATACATTGTGTGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCGCCATTTCTACATTGGGCAGTTGTTCATTCTTTTAgtgt                                                                                                                  <  1:1016097/150‑1 (MQ=38)
                                   ttATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTgg                                                                                                                <  2:699963/150‑1 (MQ=255)
                                     agcTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTa                                                                                                              <  2:851840/148‑1 (MQ=255)
                                     aTCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCATTTGTTCATTATTTTATTGTGGTa                                                                                                              >  2:238484/1‑150 (MQ=255)
                                        tGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAAtt                                                                                                           <  2:373138/150‑1 (MQ=255)
                                         taaCATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTc                                                                                                          <  1:608769/149‑1 (MQ=255)
                                         gAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCAGATCTACATGGGGCAGTTGTTCATTCTTTTATTGTGGTAATTc                                                                                                          >  2:130330/1‑150 (MQ=255)
                                          aaCATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTca                                                                                                         >  2:1041795/1‑150 (MQ=255)
                                            cATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTcaca                                                                                                       <  2:98037/150‑1 (MQ=255)
                                                 aaCACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCa                                                                                                  <  1:278735/150‑1 (MQ=255)
                                                    acTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCa                                                                                               >  1:493919/1‑150 (MQ=255)
                                                      attgaGTAATGTGGAGTCATTACCGACCATGTTTAATTCATACATTGTGGTTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCaaa                                                                                             <  1:196436/146‑1 (MQ=255)
                                                             aaGTTGGATTCATTACCGACCATTTTTATTTCATACATTGTTGGTATTTTTATTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTg                                                                                      <  1:640881/150‑1 (MQ=255)
                                                                ttGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTTTGGTAATTCACACGCCAGCAAAAACTCTGCCg                                                                                   >  2:564946/1‑150 (MQ=255)
                                                                 tGGAGTCATTACCGCCCATGTTTATTTCATAAATACTGGGTATTGTTCTTATTATCGCCGCTTATCAATAAAATCCTGCCCCATATCTAGATGGCGCAGCTGGACATTCTTTTAGTGTGGTAAATCACACGCCAGCACAAACTCTGCCGt                                                                                  >  2:471938/1‑150 (MQ=255)
                                                                     gTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCt                                                                              >  1:292380/1‑150 (MQ=255)
                                                                     gTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCt                                                                              >  2:439429/1‑150 (MQ=255)
                                                                         ttACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcat                                                                          <  2:717019/150‑1 (MQ=255)
                                                                         ttACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcat                                                                          <  1:341502/150‑1 (MQ=255)
                                                                           aCCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcatca                                                                        >  2:378282/1‑150 (MQ=255)
                                                                            ccGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaa                                                                       <  1:124511/150‑1 (MQ=255)
                                                                              gACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAACATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaaca                                                                     <  2:808417/150‑1 (MQ=255)
                                                                              gACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaaca                                                                     <  1:731778/150‑1 (MQ=255)
                                                                                 cATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAAATCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATc                                                                  <  2:686293/150‑1 (MQ=255)
                                                                                  aTGTTTATTTCATACATTGTGTTTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCAATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCa                                                                 <  1:168592/150‑1 (MQ=255)
                                                                                  aTGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCa                                                                 <  2:1298114/150‑1 (MQ=255)
                                                                                  aTGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGAAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCAGTTCCTTCATCAACAATCa                                                                 >  1:755990/1‑150 (MQ=255)
                                                                                    tttaatatCATACATTGTGGGTATGGTCCTTATTATCGCCGCTACTCAATAAACTCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCATTTACTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCCTCAACAATCAgg                                                               <  2:30933/143‑1 (MQ=255)
                                                                                    gTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAgg                                                               >  1:903543/1‑150 (MQ=255)
                                                                                    gTTAATATCCTACATTGTGGGTCTTGATCTTATTACCGCCGCTAATCAATAACATCCTTCCCCATATCTACATGGGGCAGTTGTGCATTCTTTTAGTGTGGTAAATCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAgg                                                               <  2:150662/150‑1 (MQ=255)
                                                                                      ttATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTc                                                             <  1:313544/150‑1 (MQ=255)
                                                                                        aTTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCg                                                           <  2:561192/150‑1 (MQ=255)
                                                                                        aTTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCg                                                           <  2:1122232/150‑1 (MQ=255)
                                                                                          ttCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTg                                                         >  1:489794/1‑150 (MQ=255)
                                                                                            cATACAAAGTGGGTATACTTCTTATTATCGCCGCAAATCAAAAAAATCCTGCCCCATATCTACATGGGGCAGTTGTACATTCTTTTAGTGTGGTATTTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAAACAGGTCCGTGAc                                                       >  2:22424/1‑150 (MQ=255)
                                                                                             aTACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGaaa                                                      >  1:926957/1‑148 (MQ=255)
                                                                                             aTACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACa                                                      <  2:1236593/150‑1 (MQ=255)
                                                                                                  ttGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATAtcc                                                 <  2:35127/150‑1 (MQ=255)
                                                                                                  ttGTGGGTATTGTTCTTATTATCGCCGCTAATCAAAAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATAtcc                                                 <  1:238484/150‑1 (MQ=255)
                                                                                                     tgGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCAAATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACCCGCCAGCAAAAACTCTGCCGTTACTTCATCAACAATCAGGTCCGTGACATAtcctcc                                              >  2:833514/1‑150 (MQ=255)
                                                                                                      gggTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTccc                                             <  1:57912/150‑1 (MQ=255)
                                                                                                      gggTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTGCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTccc                                             <  2:108478/150‑1 (MQ=255)
                                                                                                        gTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCcag                                           >  2:321760/1‑150 (MQ=255)
                                                                                                        gTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCcag                                           >  2:739223/1‑150 (MQ=255)
                                                                                                            tGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAgg                                       <  1:263355/150‑1 (MQ=255)
                                                                                                             gTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAggg                                      <  2:330524/150‑1 (MQ=255)
                                                                                                             gTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAggg                                      >  2:831939/1‑150 (MQ=255)
                                                                                                               tCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAACAAATCTGCCGTTCCTTCATCACCAATCAGGTCCGTGACAAATCCTCCCAGCAGGGCa                                    >  2:949320/1‑150 (MQ=255)
                                                                                                                      atCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACg                             >  2:697976/1‑150 (MQ=255)
                                                                                                                        cgccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGGGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCAGCACCAATCAGGGCCGTGCGATATCCTCCCAGCAGGGCCCCCAACGtt                           >  2:208862/1‑150 (MQ=255)
                                                                                                                         gccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTg                          <  1:1078095/150‑1 (MQ=255)
                                                                                                                          ccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGc                         <  2:551841/150‑1 (MQ=255)
                                                                                                                               aaTCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATAAACAATAAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCaa                    >  1:1053230/1‑149 (MQ=255)
                                                                                                                                aTCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATa                   <  2:355659/150‑1 (MQ=255)
                                                                                                                                 tCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAg                  >  2:198444/1‑150 (MQ=255)
                                                                                                                                   aaTAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACGCACCGTTGCGTCATAGcc                >  1:931111/1‑150 (MQ=255)
                                                                                                                                    aTAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAGCATTCAGGTCCGTGACATATCCTCCCAGCAGGGCCCCCTAGCTTGCGTCCTGGccg               >  2:208498/1‑149 (MQ=255)
                                                                                                                                    aTAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCct               >  2:1128306/1‑150 (MQ=255)
                                                                                                                                        acaacTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGGTCCTTCATCAACAATCAGGTCCGGGACATATCCTCCCAGCAGGGCACCCAACGTGGCGTCATTGACGCttt           >  1:1097664/5‑150 (MQ=255)
                                                                                                                                         caacTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTc          >  1:904885/4‑150 (MQ=255)
                                                                                                                                         aTCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTc          <  1:1261154/150‑1 (MQ=255)
                                                                                                                                          tCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc         <  1:1073756/150‑1 (MQ=255)
                                                                                                                                          aacTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc         >  2:1211734/3‑150 (MQ=255)
                                                                                                                                          aacTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc         >  1:138439/3‑150 (MQ=255)
                                                                                                                                           ccTGCCCCATAGCTACATGGGGCAGTTGGTCATTCTGTTAGTGTGGTAATTCACACGCCAGCAAAACCTCTGCCGTTCCTGCATCAGCAAGCCGGGCCGGGCCATATCCGCCCCGCAGGGCCCCCACCGTGGCGGCCTAGCGGCGTTccc        >  2:523345/1‑150 (MQ=255)
                                                                                                                                           acTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTccc        >  2:585453/2‑150 (MQ=255)
                                                                                                                                              gCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCCTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccac     >  2:1146816/1‑150 (MQ=255)
                                                                                                                                               ccccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacc    >  2:297685/1‑150 (MQ=255)
                                                                                                                                               ccccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacc    <  2:1072870/150‑1 (MQ=255)
                                                                                                                                                cccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacca   >  2:477221/1‑150 (MQ=255)
                                                                                                                                                 ccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTACCCGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAg  >  1:75410/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
AAATCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAG  >  NC_000913/4519162‑4519456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: