Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,532,534 G→C 5.4% A217G (GCC→GGC)  yjhP ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,532,5340GC 5.4% 285.5 / 6.0 92A217G (GCC→GGC) yjhPputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (41/46);  new base C (3/2);  total (44/48)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

CAAAGGTAAAGCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGC  >  NC_000913/4532388‑4532683
                                                                                                                                                  |                                                                                                                                                     
caaagGTAAAGCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGGGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTcggcgg                                                                                                                                                    <  2:150715/150‑1 (MQ=255)
  ccgggaaaGCCTCGATCTTTCCCGCCAGAAATACCGGGCACCTGGTGGTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGgcggcg                                                                                                                                                  <  1:1181495/145‑1 (MQ=255)
     gTAAAGCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAg                                                                                                                                               <  1:1151300/150‑1 (MQ=255)
       aaaGCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGTGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTc                                                                                                                                             <  1:1302434/150‑1 (MQ=255)
          gCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGCCGCCGAAGTCagg                                                                                                                                          <  1:355775/150‑3 (MQ=255)
           ccTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCg                                                                                                                                         <  1:244808/150‑1 (MQ=255)
           ccTCGATATATCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGGTTCGGCGCGATGTATAGTTCGGCCCTGACTTCGCCGCCGAAGTCaggg                                                                                                                                         <  2:673263/150‑4 (MQ=255)
            cTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCgg                                                                                                                                        >  1:394313/1‑149 (MQ=255)
             tCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTc                                                                                                                                       >  2:278822/1‑150 (MQ=255)
               gATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTAAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAg                                                                                                                                     >  2:687388/1‑150 (MQ=255)
                atatTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAgg                                                                                                                                    >  2:1299542/1‑150 (MQ=255)
                atatTTCCAGCCAGAAATACCGGGAACCTGTCGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCCTCGTCAgg                                                                                                                                    >  2:252929/1‑150 (MQ=255)
                 tatTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGGCAGGa                                                                                                                                   >  1:800344/1‑150 (MQ=255)
                  atatCCAGCCATAAATACCGGGAACCTGTTGTTACCGGGCGATGCGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGAt                                                                                                                                  <  2:131239/147‑1 (MQ=255)
                      ccagccagAAATACCGGGAACCTGTTGCTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGc                                                                                                                              <  1:505100/150‑1 (MQ=255)
                       cagccagAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCt                                                                                                                             <  1:166737/150‑1 (MQ=255)
                            agAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGCTTCGCTTcaag                                                                                                                        >  2:207054/1‑150 (MQ=255)
                            agAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTccag                                                                                                                        <  2:1191589/150‑1 (MQ=255)
                              aaaTACCGGGAACCTGTTGTAACCGGGCGATGAGTGCGAACACGCCCCAGACAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTccagcc                                                                                                                      <  1:230650/150‑1 (MQ=255)
                                 tACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCcagccagc                                                                                                                   <  1:302158/150‑1 (MQ=255)
                                 tACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCcagccagc                                                                                                                   <  2:381308/150‑1 (MQ=255)
                                  aCCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAgcg                                                                                                                  >  2:504110/1‑150 (MQ=255)
                                        aaCCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCAt                                                                                                            <  1:501075/150‑1 (MQ=255)
                                         aCCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATg                                                                                                           >  2:1022576/1‑150 (MQ=255)
                                            tgttgtTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTc                                                                                                        <  2:40447/150‑1 (MQ=255)
                                            tgttgtTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTc                                                                                                        >  2:696561/1‑150 (MQ=255)
                                            ggttgttACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGGAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTc                                                                                                        <  1:366342/149‑1 (MQ=255)
                                                gttACCGGGCGATGAGTGCGAACACGCCCCAGCAATAGCATTCCCTAGCGTAGGTCACGTAGCGTTTCGGCGCGATGTATAGTTCGGCCCTGACTTCGGCGTCGAAGACATCGTCAGGATTCGCTTCCAGCCAGCGGCGCAAGGTCAGcc                                                                                                    >  2:1106349/1‑150 (MQ=255)
                                                gtaaCCGCGCGATGAGTCCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTACGGCGCGATGTATAGTTCGGCCCAGACTTCGGCGGCGAAGACATCGACAAGATTCGCTACCACCCAGCGGCGCATGGTCAGcc                                                                                                    >  2:1101069/4‑150 (MQ=255)
                                                  tACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCAt                                                                                                  >  2:133994/1‑150 (MQ=255)
                                                   aCCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCAtt                                                                                                 >  1:1171813/1‑150 (MQ=255)
                                                          gATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGCCTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCCGCCAGCGGCGCATGGTCAGCCATTTTGCGGc                                                                                          >  1:752958/1‑150 (MQ=255)
                                                           aTGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCt                                                                                         >  2:1212479/1‑150 (MQ=255)
                                                            tGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGCCTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCCGCCATTTTGCGGCtt                                                                                        >  1:46841/1‑150 (MQ=255)
                                                             gAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTc                                                                                       <  1:669262/150‑1 (MQ=255)
                                                               gTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCAt                                                                                     >  2:396975/1‑150 (MQ=255)
                                                                 gCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATAc                                                                                   >  2:537407/1‑150 (MQ=255)
                                                                 gCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATAc                                                                                   >  2:542215/1‑150 (MQ=255)
                                                                  cGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATAcc                                                                                  >  2:785435/1‑150 (MQ=255)
                                                                  cGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATAcc                                                                                  >  2:972517/1‑150 (MQ=255)
                                                                    aaCACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCgg                                                                                <  1:284089/150‑1 (MQ=255)
                                                                     acacGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTCGCGTTTCGGCGCGATGTTTAGGTCGGCCCTGACTTCGGCGGCGAAGTCATCGGCAGGATTCGCTTCCAGCCAGCGGCGCATGCTCAGCCATTTTGCGGCTTCATACCGGt                                                                               <  2:881569/150‑1 (MQ=255)
                                                                      cacGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTc                                                                              <  1:1206869/150‑1 (MQ=255)
                                                                        cGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTccc                                                                            <  2:140462/150‑1 (MQ=255)
                                                                         gCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCa                                                                           <  1:696561/150‑1 (MQ=255)
                                                                         gCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCa                                                                           >  1:1240965/1‑150 (MQ=255)
                                                                         gCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCa                                                                           >  2:514359/1‑150 (MQ=255)
                                                                         gCCCCAGCCAAAGCAGTCCCGCGCGTAGGTCACGTCGCGGTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCa                                                                           <  2:1029273/150‑1 (MQ=255)
                                                                          ccccAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAg                                                                          >  2:302626/1‑150 (MQ=255)
                                                                          ccccAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAg                                                                          <  1:933146/150‑1 (MQ=255)
                                                                                 cAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCCGCCTTCCt                                                                   >  1:982274/1‑150 (MQ=255)
                                                                                 cAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCt                                                                   <  1:305413/150‑1 (MQ=255)
                                                                                 cAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCt                                                                   <  1:21563/150‑1 (MQ=255)
                                                                                 cAAAGCATTCCCGCGCGTAGCTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGAATCCCTTCCCGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTccg                                                                   >  2:714200/1‑149 (MQ=255)
                                                                                   aaGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTgg                                                                 <  2:218432/150‑1 (MQ=255)
                                                                                      cATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGc                                                                                             >  2:239010/1‑119 (MQ=255)
                                                                                      cATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGc                                                                                             <  1:239010/119‑1 (MQ=255)
                                                                                       aTTCCCGCGCGTAGGTCACGAAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTg                                                             <  2:1107402/150‑1 (MQ=255)
                                                                                         tCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGcc                                                           <  1:884773/150‑1 (MQ=255)
                                                                                          cccGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCAGTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCa                                                          >  1:71622/1‑150 (MQ=255)
                                                                                                cgTAGGTCACGTAGCGTTTCGTCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGGCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCa                                                    <  2:78560/150‑1 (MQ=255)
                                                                                                cgTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGGCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCa                                                    <  2:739539/150‑1 (MQ=255)
                                                                                                    ggTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTc                                                <  1:411979/150‑1 (MQ=255)
                                                                                                     gTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTcc                                               <  2:726121/150‑1 (MQ=255)
                                                                                                         cGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTccacca                                           <  2:18456/150‑1 (MQ=255)
                                                                                                         cGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTccacca                                           >  1:1158040/1‑150 (MQ=255)
                                                                                                         cGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATGTccacca                                           >  1:927040/1‑150 (MQ=255)
                                                                                                          gTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCcaccac                                          <  1:63439/150‑1 (MQ=255)
                                                                                                               gTTTCGGCGCGATGTTTAGTTCGGCCCTGCCTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGGCTGCCTGTACCAGGTCCCCCACGTCAt                                     >  2:802553/1‑150 (MQ=255)
                                                                                                                 ttCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAg                                   <  1:963157/150‑1 (MQ=255)
                                                                                                                 ttCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAg                                   <  1:618514/150‑1 (MQ=255)
                                                                                                                 ttCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAg                                   <  2:835458/150‑1 (MQ=255)
                                                                                                                    ggCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCg                                <  2:267901/150‑1 (MQ=255)
                                                                                                                      cgcgATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTACCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTCCCATTTCCACCACGTCATAGCCgag                              >  2:571456/1‑150 (MQ=255)
                                                                                                                        cgATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGAt                            <  1:1108134/150‑1 (MQ=255)
                                                                                                                        cgATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGGCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGAt                            <  2:1069204/150‑1 (MQ=255)
                                                                                                                         gATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGAtc                           >  2:1169570/1‑150 (MQ=255)
                                                                                                                         gATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGAtc                           <  2:1154604/150‑1 (MQ=255)
                                                                                                                         gATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGAtc                           <  2:1067122/150‑1 (MQ=255)
                                                                                                                            gTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGAtcgtc                        >  2:1168084/1‑150 (MQ=255)
                                                                                                                                 gTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGGCTGCCTGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACg                   <  2:868732/150‑1 (MQ=255)
                                                                                                                                   tCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCt                 >  2:139298/1‑150 (MQ=255)
                                                                                                                                   tCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCt                 >  1:387923/1‑150 (MQ=255)
                                                                                                                                     ggCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTcc               >  2:486482/1‑150 (MQ=255)
                                                                                                                                      gCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCTGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACTCTCCg              <  1:335697/150‑1 (MQ=255)
                                                                                                                                      gCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCg              >  2:80437/1‑150 (MQ=255)
                                                                                                                                      acgatGACTTCGCCGCCGAAGTCATCGTCTGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCGCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCTCGTCATAGCCGAGATCGTCGAACGCTCCg              >  1:537560/5‑150 (MQ=255)
                                                                                                                                      acgatGACTTCGCCGCCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTACg              >  1:524316/5‑150 (MQ=255)
                                                                                                                                       ctatGACTTCGCCGCCGAAGTCATCGTCAGGATTCGCTTCCCGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCCGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGa             >  1:991694/4‑150 (MQ=255)
                                                                                                                                       cccTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCgc             >  1:1180123/1‑149 (MQ=255)
                                                                                                                                         cTGTCTTCGGAGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCTGCTTCATACAGGTCCCATCCTTCCTGGTCTGCCAGTAACATTTCCACCACTTCATAGCCGAGATCGTCGAACGCTCCGACa           <  1:200937/150‑1 (MQ=255)
                                                                                                                                         cTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACa           >  2:485503/1‑150 (MQ=255)
                                                                                                                                           gACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAg         <  1:1026003/150‑1 (MQ=255)
                                                                                                                                               tCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCAGGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCt     <  1:824965/150‑1 (MQ=255)
                                                                                                                                                  gcggcgAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGc  <  1:522573/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAAAGGTAAAGCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCGAGATCGTCGAACGCTCCGACAAGTCCTGGC  >  NC_000913/4532388‑4532683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑