Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 566,367 C→T 8.4% pseudogene (10/279 nt) exoD ← pseudogene, DLP12 prophage; phage‑type exonuclease family;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913566,3670CT 8.4% 289.4 / 10.5 95pseudogene (10/279 nt)exoDpseudogene, DLP12 prophage; phage‑type exonuclease family;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (51/36);  new base T (5/3);  total (56/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.19e-01

ACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAG  >  NC_000913/566219‑566516
                                                                                                                                                    |                                                                                                                                                     
aCATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATcc                                                                                                                                                      <  2:411140/150‑1 (MQ=255)
   tAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCgg                                                                                                                                                   <  2:1009555/150‑1 (MQ=255)
   tAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCgg                                                                                                                                                   >  2:498460/1‑150 (MQ=255)
    aaTGCATGCCTTAACGCTGCATGCGAGGTTCATTGTTTGCAACGTACCAGGCATCATTTCGCGTCACCCACATGCTGTACTTCACCTTGGCCATGTAAGCCGATTTTATTGCCTCGAAACGACCGAGCCGGAATTTCATGAAATCCCggg                                                                                                                                                  <  1:857734/150‑1 (MQ=255)
    aaTGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGTCCATGTAAGCCGATTTATTTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCggg                                                                                                                                                  <  2:592721/150‑1 (MQ=255)
      tGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGGATTTCATGAAATTCCggctc                                                                                                                                                <  1:486580/150‑4 (MQ=255)
      tGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAg                                                                                                                                                <  2:970610/150‑1 (MQ=255)
       gCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAgg                                                                                                                                               >  1:602764/1‑150 (MQ=255)
       gCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAgg                                                                                                                                               <  2:594338/150‑1 (MQ=255)
        cAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGt                                                                                                                                              >  2:270575/1‑150 (MQ=255)
          ggCCTTCACGCTTCATGCGCGGGTCATAGTTGGCATAGTACCAGGCTTCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGGATTTCATGAAATTCCggctcggtg                                                                                                                                            <  2:582930/150‑8 (MQ=255)
              ttCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGt                                                                                                                                        <  2:279113/150‑1 (MQ=255)
              ttCACGCTTCATGCGCGGGGCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGt                                                                                                                                        <  2:259718/150‑1 (MQ=255)
                cACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCGCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTAt                                                                                                                                      >  2:21679/1‑150 (MQ=255)
                    cTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGcc                                                                                                                                  >  2:1211099/1‑150 (MQ=255)
                       cATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCAGGa                                                                                                                               >  1:1201355/1‑150 (MQ=255)
                               ggTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGc                                                                                                                       >  1:604159/1‑150 (MQ=255)
                                 tCATAGTTGGGAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCGGTACTGCACCTGGTCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCt                                                                                                                     <  1:119203/150‑1 (MQ=255)
                                 tCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACCTTCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGTAATTTCATGAAATCCCGGGAGGTACTAGTATTGCCGGAAGCGTGTCCt                                                                                                                     <  2:462964/150‑1 (MQ=255)
                                  cATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTg                                                                                                                    <  2:869583/150‑1 (MQ=255)
                                  cATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTg                                                                                                                    <  2:882972/150‑1 (MQ=255)
                                      gTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATc                                                                                                                <  2:1081050/150‑1 (MQ=255)
                                        tGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCg                                                                                                              >  1:909423/1‑150 (MQ=255)
                                          gCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGTGCCATGTAAGCCGAGTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGa                                                                                                            >  2:1017342/1‑150 (MQ=255)
                                          gCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGCAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGa                                                                                                            >  2:804439/1‑150 (MQ=255)
                                            aaaGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATg                                                                                                          <  1:1284830/150‑1 (MQ=255)
                                            aaaGTACCAGGCATCTTTTCGCGTCACCCACATGCTGGACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCAACGAGCCGGTATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATg                                                                                                          <  2:524022/150‑1 (MQ=255)
                                               gTACCAGGCAGCTTCTCGCGTCACCCCCATGCTGTACTGCACCTGGGCCATGTAAGCCGAGTTTATTGCCGCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATAGCCGGAAGCGGGGCCTGTATCCGGATGCag                                                                                                       <  1:1050742/150‑1 (MQ=255)
                                                  ccAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAACCCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGt                                                                                                    >  1:319142/1‑150 (MQ=255)
                                                   cAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTc                                                                                                   <  2:596466/150‑1 (MQ=255)
                                                    aGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGtat                                                                                                  >  2:642214/1‑148 (MQ=255)
                                                    aGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCt                                                                                                  >  1:1027210/1‑150 (MQ=255)
                                                    aGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCt                                                                                                  <  1:350123/150‑1 (MQ=255)
                                                    aGGCATATTTTCCCGACACCCACATTCTGTACTGCAACTGGGCCATGAAAGGCCATGTTATTGCCTCGAACCCCCCGCGCCGGAAATTCATCAAATCCCGGGACGTAAGAGAATTGCCGGAACCGTGGCCTGTATCCGGATGCAGAGtgt                                                                                                  >  2:981838/1‑148 (MQ=255)
                                                     ggCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCtt                                                                                                 <  2:352593/150‑1 (MQ=255)
                                                           ttttCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGACTCGAAACCACCGAGCCGCAATTTCATGAATTCCCGGGAGGTACGAGTATTGCCGGAAGCCTGGCCTGTATCCGGATGAAGAGACTTATcagg                                                                                           >  1:176897/1‑147 (MQ=255)
                                                            tttCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTg                                                                                          <  1:228256/150‑1 (MQ=255)
                                                             ttCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTgg                                                                                         >  2:400776/1‑150 (MQ=255)
                                                             ttCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTgg                                                                                         <  1:349773/150‑1 (MQ=255)
                                                             ttCGCGGCACCCCCCTGCTGTACTGCACCTGGGCAATGTAAGCCGATTTTATTGCCTCGCAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTTGCCTGTATCCGGATGCAGAGTCTTATCCGTgg                                                                                         <  1:818640/150‑1 (MQ=255)
                                                                    cACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGa                                                                                  >  2:7332/1‑150 (MQ=255)
                                                                              cTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTa                                                                        >  1:1264214/1‑150 (MQ=255)
                                                                              cTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTa                                                                        <  1:1172252/150‑1 (MQ=255)
                                                                               tGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCAGTGGAAATCGAACGCGCATTAc                                                                       >  2:398099/1‑150 (MQ=255)
                                                                                gTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACt                                                                      >  1:547088/1‑150 (MQ=255)
                                                                                  gcTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGCGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATCACtgg                                                                    >  1:447926/2‑150 (MQ=255)
                                                                                  aCTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACtgg                                                                    >  1:826681/1‑150 (MQ=255)
                                                                                    tGCCCCTGGGCCATGTAAGCCGAGTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACtggtt                                                                  <  2:107327/150‑1 (MQ=255)
                                                                                      cACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACtggttgg                                                                <  1:14880/150‑1 (MQ=255)
                                                                                        ccTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTggttggtt                                                              >  1:1199643/1‑150 (MQ=255)
                                                                                        ccTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTggttggtt                                                              >  2:1154490/1‑150 (MQ=255)
                                                                                        ccTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCCGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTggttggtt                                                              >  1:33268/1‑150 (MQ=255)
                                                                                         cTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGATCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTa                                                             <  1:875749/150‑1 (MQ=255)
                                                                                         cTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAAACGAACGCGCATTACTGGTTGGTTa                                                             >  1:148639/1‑150 (MQ=255)
                                                                                            ggCCATGTAAGCAGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCa                                                          <  1:1215440/150‑1 (MQ=255)
                                                                                             gCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTTCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCTTGGAAATCGAACGCGCATTACTGGTTGGTTTCCaa                                                         >  1:832176/1‑150 (MQ=255)
                                                                                             gCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCaa                                                         >  2:80896/1‑150 (MQ=255)
                                                                                               cATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACt                                                       <  2:947051/150‑1 (MQ=255)
                                                                                               cATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACt                                                       >  2:17169/1‑150 (MQ=255)
                                                                                                   tAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTa                                                   >  2:1309766/1‑150 (MQ=255)
                                                                                                       ccGATTTTATTGCCTCGAAACCACCGCGCCGGACTTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGGTGGTTACCAACTTGTACCAg                                               <  2:1281632/150‑1 (MQ=255)
                                                                                                        cGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGa                                              <  2:602764/150‑1 (MQ=255)
                                                                                                         gATTTTATTGCCTCGATACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGTAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGaa                                             <  1:932281/150‑1 (MQ=255)
                                                                                                         gATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGaa                                             >  1:705156/1‑150 (MQ=255)
                                                                                                             ttATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACAAACTTGTACCAGAACATg                                         >  1:666223/1‑150 (MQ=255)
                                                                                                               atagCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTGATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCTACTTGTCCCAGAACTTGCg                                       >  2:1213921/4‑150 (MQ=255)
                                                                                                               aTTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCg                                       >  2:110161/1‑150 (MQ=255)
                                                                                                               aTTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCg                                       <  1:21679/150‑1 (MQ=255)
                                                                                                                  gCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAGCAGAACATGCGGGc                                    >  2:1243346/1‑150 (MQ=255)
                                                                                                                   ccgcGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGcc                                   <  1:238655/147‑1 (MQ=255)
                                                                                                                   ccTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCGTGTATCCGGATGCAGAGTCTTTGCCGTGGAATTCGAACGCGGATTACTGGTTGGTTACCATCTGGGCCCCGAACCTGCggggc                                   >  2:191909/1‑148 (MQ=255)
                                                                                                                   ccTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGcc                                   >  1:667354/1‑150 (MQ=255)
                                                                                                                           ccaccGAGCCGGAATTTCATGAAATCCCGGGAGGGACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgct                           >  2:856516/1‑150 (MQ=255)
                                                                                                                            caccGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctg                          <  1:711990/150‑1 (MQ=255)
                                                                                                                             accGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGAgctgg                         >  1:504261/1‑150 (MQ=255)
                                                                                                                                gAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctggctg                      >  1:262163/1‑150 (MQ=255)
                                                                                                                                ctcccGGGATTTCATGAAATTCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctggctg                      >  2:1192373/4‑150 (MQ=255)
                                                                                                                                  cccGGGATTTCATGAAATTCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctggctgcc                    >  2:1126260/2‑148 (MQ=255)
                                                                                                                                  cccGGGATTTCATGAAATTCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGAc                    >  2:682293/2‑150 (MQ=255)
                                                                                                                                  cccGGGATTTCATGAAATTCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTAATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTAACAACTTGTACCAGAACATGCGGGCCAATCCGCTGGCTGAc                    >  2:92589/2‑150 (MQ=255)
                                                                                                                                   ccGGGATTTCATGAAATTCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGAcg                   >  1:320625/1‑150 (MQ=255)
                                                                                                                                   ccGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGAcg                   >  1:516837/1‑150 (MQ=255)
                                                                                                                                   ccGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGGGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGACCATGCGGGCCACTGCGCTGACTGAcg                   >  2:65205/1‑150 (MQ=255)
                                                                                                                                   ccGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCATGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTCCCACCTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGAcg                   >  2:100571/1‑150 (MQ=255)
                                                                                                                                     ggAATTTCTTGAAATCCCGGGAGGTACGAGTATTGCCGGTAGCGTGGCCTGTATCCGGATGCAGAGTGTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGAcgcg                 <  2:1001454/150‑1 (MQ=255)
                                                                                                                                     ggAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGAcgcg                 >  2:615803/1‑150 (MQ=255)
                                                                                                                                     ggAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGAcgcg                 >  2:360711/1‑150 (MQ=255)
                                                                                                                                        aTTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGCACGCGCATTACTGGTTGTTTACCAACTTGTACCAGAACATGCGGGCCACTGCGCTGGCTGACGCGGaa              >  2:1114365/1‑150 (MQ=255)
                                                                                                                                         tttCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGCGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACCTGCGGGCCAATGCGCTGGCTGACGCGGAAt             <  2:182174/150‑1 (MQ=255)
                                                                                                                                          ttCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCATAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAAtt            <  2:682346/150‑1 (MQ=255)
                                                                                                                                            cATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTAc          >  2:240766/1‑150 (MQ=255)
                                                                                                                                            cATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTAc          >  1:391442/1‑150 (MQ=255)
                                                                                                                                              tGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTTTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACgg        >  1:882642/1‑150 (MQ=255)
                                                                                                                                              tGAAATCCAGGGAGGTACGAGTATTGCCGTAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGATCGCGCTTTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACgg        >  1:338131/1‑150 (MQ=255)
                                                                                                                                                aaaTTCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGgcg      <  2:320625/150‑1 (MQ=255)
                                                                                                                                                  aTCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCa    >  2:478861/1‑150 (MQ=255)
                                                                                                                                                    cccGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGAGCTGGCTGACGCGGAATTACGGCCCAAg  >  2:1006998/1‑150 (MQ=255)
                                                                                                                                                    cccGGGAGGTACGAGTATTGCCGGAAGCGGGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAg  <  2:9885/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAG  >  NC_000913/566219‑566516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: