Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 675,408 C→A 10.6% H131N (CAC→AAC)  ybeL → DUF1451 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913675,4080CA10.6% 116.8 / 3.2 50H131N (CAC→AAC) ybeLDUF1451 family protein
Reads supporting (aligned to +/- strand):  ref base C (29/15);  new base A (2/4);  total (31/19)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATTAAAGAAAGCTTGTGGCAGGAGCTGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCC  >  NC_000913/675259‑675557
                                                                                                                                                     |                                                                                                                                                     
tGATTAAAGAAAGCTTGTGGCAGGAGCTGGCAGACATCACCGATAAAACGCAGCTAGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCTAGAAATGTc                                                                                                                                                       >  2:162271/1‑150 (MQ=255)
  aTTAAAGAAAGCTTGTGGCAGGAGCTGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCAc                                                                                                                                                     >  2:487720/1‑150 (MQ=255)
   ttAAAGAAAGCTTGTGGCAGGAGCTGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACt                                                                                                                                                    >  2:522532/1‑150 (MQ=255)
             cTTGTGGCAGGAGCTGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGGTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTccc                                                                                                                                          <  1:1287520/150‑1 (MQ=255)
              ttGTGGCAGGAGCTGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGGCGGGCTGGGAAATCTGGGCTGCGAGAAATGTCACTTCCATCTCCCg                                                                                                                                         <  1:1102629/150‑1 (MQ=255)
               tgtgGCAGGAGCTGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGa                                                                                                                                        >  2:1037961/1‑150 (MQ=255)
                  ggCAGGAGCTGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCt                                                                                                                                     <  1:613528/150‑1 (MQ=255)
                     aggctcTGGCAGACATCAACTATAAAAGGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGGTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTaca                                                                                                                                  <  2:372383/145‑1 (MQ=255)
                           tGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCgg                                                                                                                            >  2:1307194/1‑150 (MQ=255)
                            ggCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATAACAGCGGAGAACTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTAACTTCCATCTCACGATCTACAAACCGGa                                                                                                                           >  1:1154693/1‑150 (MQ=255)
                                     cACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGAc                                                                                                                  >  2:546700/1‑150 (MQ=255)
                                        cGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGTACCTCAATCATAATGGGGTTTATCAAAGCGGAGAAGTGTTCGGGATGGGAAAACTGGTCTGCGAGAAATGTCACATCCATATCCCGATCTACACACCGGAAGTGCTGACgat                                                                                                               >  2:65292/1‑148 (MQ=255)
                                            aaaaCGCAGCTTGAATGGCGCGAAGTATCCCAGGACCTCAATCATCATGCGCTTTATCACAGCGGAGAAGTGGTCGCGCGGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGc                                                                                                           <  1:668277/150‑1 (MQ=255)
                                             aaaCTCAGCTTTAATGGCTCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGTTCGGGATTGGAAATCTTTTTTTTTTTAATGTTAAGTTGAATATCGAGATCTACACACCGGAAGTGCTGACGCTATGcc                                                                                                          <  2:389653/150‑1 (MQ=255)
                                             aaaCGCAGCTTGATTGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTACATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGcc                                                                                                          >  1:320854/1‑150 (MQ=255)
                                              aaCGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGccc                                                                                                         >  1:1048143/1‑150 (MQ=255)
                                                  cAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGaaa                                                                                                     <  1:241843/150‑1 (MQ=255)
                                                       tGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATTTGGTTTATCACACCGGAGAAGTGGTCGGGCTGGTAAATTTTTTTTTTTTTAATTTTAAGTTGAATATCGAGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTgg                                                                                                <  2:1101049/150‑1 (MQ=255)
                                                         aaTGGCGCGAAGTTTTCCATGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTTTTTTTTTTTAATTTTAAGTTGAATATCGAGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTc                                                                                              <  2:1067551/150‑1 (MQ=255)
                                                                 gAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAg                                                                                      <  1:405254/150‑1 (MQ=255)
                                                                         ccAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGACACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGCTCATGCCCAGTACCagag                                                                              >  1:1264421/1‑150 (MQ=255)
                                                                          cAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCTATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCAAgaga                                                                             >  2:778961/1‑150 (MQ=255)
                                                                          cAGGACCTCAATCATAATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCT‑GGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCTGAAGTGCTGACGCTATGCCAGAAATGTGTTCATGACCAGTTCCAGAGAc                                                                            >  2:141228/1‑150 (MQ=255)
                                                                            tgACCTCACTCCTCATGGTGTCTATCACAGCGGAGAAGTGGTCTGGCTGGGAAATAGTGTCTGCGAGACAAGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACTCTATGCCCGAACTGTGGGCATGACCAGTTCCAGAGACg                                                                           <  1:236386/149‑1 (MQ=255)
                                                                            ggACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTAACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACg                                                                           >  2:831755/1‑150 (MQ=255)
                                                                             gACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTAGGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGc                                                                          >  1:654514/1‑150 (MQ=255)
                                                                               ccTCAATCATCATGGGGTTTATCACAGTGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGccc                                                                        >  2:83809/1‑150 (MQ=255)
                                                                                 tCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGt                                                                      >  2:1068670/1‑150 (MQ=255)
                                                                                 gcAATCCTCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAAGGGCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGt                                                                      <  1:320665/149‑1 (MQ=255)
                                                                                      catcatGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAg                                                                 >  1:950402/1‑150 (MQ=255)
                                                                                       atcatGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGc                                                                <  1:533405/150‑1 (MQ=255)
                                                                                                            gagaAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGcac                                           >  2:968393/1‑150 (MQ=255)
                                                                                                               aaGTGGGCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGc                                        <  1:465097/150‑1 (MQ=255)
                                                                                                                 gTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACAAGTTCCAGAGACGCCCGTATGAGCCGTAATAGTCTGATTTCGCACAGCaa                                      >  1:343333/1‑150 (MQ=255)
                                                                                                                   ggTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATa                                    >  2:963722/1‑150 (MQ=255)
                                                                                                                     tCGGGCTGGGAAATCTGTTTTTTGTTAATTTTAAGTTGAATATCGAGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATaaa                                  <  2:256155/150‑1 (MQ=255)
                                                                                                                       gggCTGGGAACTCTGGTCTGCGATAAATGTCACTTCCAGCTCCCGATCTACACACCTGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAaga                                <  1:162271/150‑1 (MQ=255)
                                                                                                                        ggCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAATTGCTGACTCTATGCCCGATATGTTGTCATGAACATGTCCAGAGCCGCCCGTTAGAGCCGTAATAGACTGATTTCGCAAAGCAATAAagat                               >  2:79054/1‑149 (MQ=255)
                                                                                                                          cTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGaa                             >  2:82956/1‑150 (MQ=255)
                                                                                                                            gggAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAata                           >  1:775363/1‑150 (MQ=255)
                                                                                                                              gAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTACGCACAGCAATAAAGAGAATATg                         >  1:152065/1‑150 (MQ=255)
                                                                                                                               aaaTCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATTACCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGAAATAGTATGATTTCTCACAGCAATAAAGAGAataatg                        >  2:110826/1‑147 (MQ=255)
                                                                                                                                 aTCTTGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGc                      <  1:1107277/150‑1 (MQ=255)
                                                                                                                                 aTCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGc                      <  1:436381/150‑1 (MQ=255)
                                                                                                                                   cTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCg                    >  2:1299496/1‑150 (MQ=255)
                                                                                                                                    tGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGCCCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGa                   >  1:277080/1‑150 (MQ=255)
                                                                                                                                     ggTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGaa                  >  1:690603/1‑150 (MQ=255)
                                                                                                                                           cGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGGAAGGTGGTCATGACCAGTGCCAGAGCCGCCCGTTTGAGCCGTAATAGTCGGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAAt            >  1:339093/1‑150 (MQ=255)
                                                                                                                                               aaaTGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTccc        <  1:968393/150‑1 (MQ=255)
                                                                                                                                                aaTGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTcccc       >  2:302691/1‑150 (MQ=255)
                                                                                                                                                   gTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGc    >  1:688031/1‑150 (MQ=255)
                                                                                                                                                     cACTTCCATCTCCCGATCTACACACCGGAAGTGCAGACGCTATGCCCGAAATGTGGTCATGACAAGTTCCAGAGACGCCCGTTTGCGCCGTAATAGTCTGATTTCGCACAGCAATAAAGATAATATGGGACGAATGAAATTCCCCCGccc  >  2:200948/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGATTAAAGAAAGCTTGTGGCAGGAGCTGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCC  >  NC_000913/675259‑675557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑