Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 850,993 T→C 6.4% intergenic (+28/+21) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913850,9930TC 6.4% 177.9 / 2.9 64intergenic (+28/+21)ompX/opgEouter membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base T (32/25);  new base C (2/2);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

ACGGTGCGGGTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATG  >  NC_000913/850844‑851131
                                                                                                                                                     |                                                                                                                                          
aCGGTGCGGGTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCct                                                                                                                                            >  1:1156430/1‑150 (MQ=255)
     gCGGGTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCgg                                                                                                                                       >  2:286708/1‑150 (MQ=255)
      cGGGTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCggg                                                                                                                                      >  1:480613/1‑150 (MQ=255)
      cGGGTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCggg                                                                                                                                      >  1:165074/1‑150 (MQ=255)
        ggTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGc                                                                                                                                    <  2:165074/150‑1 (MQ=255)
         gTCTGCAGATCAACCCGATGGCAAACGTTGCTCTGGACTTCTCTTCCGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCg                                                                                                                                   <  1:1111678/150‑1 (MQ=255)
          tCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTTGCTGTGTTGGTTACAGCTTCTTATCACGTTGGTGATTTAAAAAATCAGCCTCTCGgggggg                                                                                                                                  >  1:150108/1‑150 (MQ=255)
           cTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGa                                                                                                                                 >  1:722818/1‑150 (MQ=255)
            tGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAt                                                                                                                                <  2:293118/150‑1 (MQ=255)
               aGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGAtttt                                                                                                                             >  2:351649/1‑150 (MQ=255)
               aGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttt                                                                                                                             >  1:640468/1‑150 (MQ=255)
                 ttCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTg                                                                                                                           <  2:281218/150‑1 (MQ=255)
                 ttCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCCCGAGAGGCGGAtttttt                                                                                                                           <  2:640468/150‑2 (MQ=255)
                   cAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGtt                                                                                                                         >  1:371215/1‑150 (MQ=255)
                   aaccccGATTGAAAACGTAGCTCTTGACTACACTAACCAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGtt                                                                                                                         <  2:854835/147‑1 (MQ=255)
                      cccGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGttttt                                                                                                                      <  2:1039385/150‑1 (MQ=255)
                       ccGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTa                                                                                                                     >  2:875895/1‑150 (MQ=255)
                       ccGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTa                                                                                                                     <  2:439309/150‑1 (MQ=255)
                            ggAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGtt                                                                                                                >  1:1057533/1‑150 (MQ=255)
                             gAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGttt                                                                                                               >  1:716646/1‑150 (MQ=255)
                               aaaccgagCTCTGCACTTCTCTTTAGAGGATAGCCGTTATCGTTCCGTTTCCGGTGGCCCCTGGATTGCCGGTGTTGGTTTCCGCGGCTTATCACTTTGGTGATAAAAAACAACCGCCGCTCGGGGGGGATTTTTGTTTTTAAGGTTTCg                                                                                                             <  2:705794/143‑1 (MQ=255)
                                  cGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGt                                                                                                          <  2:349439/150‑1 (MQ=255)
                                            gACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGATAATATc                                                                                                >  1:944840/1‑150 (MQ=255)
                                              cTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCgg                                                                                              <  2:1137217/150‑1 (MQ=255)
                                              cTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCgg                                                                                              >  2:1128376/1‑150 (MQ=255)
                                                   ctTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGAGTTGGTTACCGCTTCTAATCACTATGGTGATATAAAAAATCCGCCTCTCGTGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCg                                                                                         >  1:698892/1‑150 (MQ=255)
                                                          gCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGt                                                                                  <  1:1064512/150‑1 (MQ=255)
                                                           cAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGtt                                                                                 >  1:981030/1‑150 (MQ=255)
                                                                   tATTCGTAGCGGTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCa                                                                         <  2:981647/150‑1 (MQ=255)
                                                                     ttCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTGGGTTACCGCGTCTAATCACTTTGGTGATATAAAAAATCCGCCGCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGGTCCGAGATGGTTGTAATCCAcc                                                                       <  1:633052/150‑1 (MQ=255)
                                                                      tCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCt                                                                      <  2:1286172/150‑1 (MQ=255)
                                                                       cGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCtt                                                                     >  1:389990/1‑150 (MQ=255)
                                                                       cGTAGCGTTGACGTAGGCACCTGGATTGCAGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCtt                                                                     >  1:961812/1‑150 (MQ=255)
                                                                               tGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACt                                                             >  1:1046783/1‑150 (MQ=255)
                                                                                 ccGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGCGGAGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTgg                                                           <  2:726612/149‑1 (MQ=255)
                                                                                      ggCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGtt                                                      <  1:1103774/150‑1 (MQ=255)
                                                                                        cACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg                                                    >  1:1048828/1‑150 (MQ=255)
                                                                                        cACCTGGATTGCCGGTGGTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTGCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTgg                                                    <  1:128831/150‑1 (MQ=255)
                                                                                        cACCTGGATTGCCGCTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTATTAAGGTTTCGCGTCGAAAATATCGGTAACGAGATGGTTGTAATCAACCTTCTGTAACTTGAAGATgg                                                    >  2:1016262/1‑150 (MQ=255)
                                                                                         aCCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAACTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGt                                                   <  2:1305249/150‑1 (MQ=255)
                                                                                          ccTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCTCCTCTAGGGTCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGCTGGTTGTAATCAACCTTCTTTAACTGGAAGTTgggg                                                  >  2:618951/1‑148 (MQ=255)
                                                                                          ccTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTTAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTg                                                  >  2:340278/1‑150 (MQ=255)
                                                                                           cTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGa                                                 >  1:649497/1‑150 (MQ=255)
                                                                                                  gCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTatat                                          >  1:52441/1‑150 (MQ=255)
                                                                                                        gtTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGccc                                    <  2:797414/150‑1 (MQ=255)
                                                                                                        gtTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGccc                                    >  1:947032/1‑150 (MQ=255)
                                                                                                         tgggTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTGTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCg                                   <  2:305957/148‑1 (MQ=255)
                                                                                                          ttgTTTCCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGAGGATTTTTGTTTTTAAGGTTTCGGGGCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGGGATGTATATCGGCCCgg                                  <  1:764569/148‑1 (MQ=255)
                                                                                                            gTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCt                                >  1:990906/1‑150 (MQ=255)
                                                                                                              tACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCttt                              >  1:1210447/1‑150 (MQ=255)
                                                                                                              tACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCttt                              <  2:829495/150‑1 (MQ=255)
                                                                                                                ccGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTttct                            >  1:1114649/1‑150 (MQ=255)
                                                                                                                  gCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTttctta                          >  2:600733/1‑149 (MQ=255)
                                                                                                                    ttCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTtctc                        <  2:1040479/150‑1 (MQ=255)
                                                                                                                      atAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATACACCTTCTGTATCTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAg                      <  1:846827/149‑1 (MQ=255)
                                                                                                                               ttGGTGATATAAAAAATCCGCCTCTCGGGGCTGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTACGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACg             <  2:878047/150‑1 (MQ=255)
                                                                                                                               ttGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACg             <  1:1151077/150‑1 (MQ=255)
                                                                                                                                   tGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATTTCGGCCCGGCTTTCTTCTCAGATATAAACGTGTa         >  1:1144626/1‑150 (MQ=255)
                                                                                                                                        taAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGa    <  2:649497/150‑1 (MQ=255)
                                                                                                                                        taAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGG‑AGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGAt   >  2:1250269/1‑150 (MQ=255)
                                                                                                                                        taAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACAGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGa    <  2:759399/150‑1 (MQ=255)
                                                                                                                                        taAAAAATCCGCCGCTCGGGGCGGAGTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTATCTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATTAACGGGTAGTTGa    >  1:1020469/1‑150 (MQ=255)
                                                                                                                                        acaaaaaTCCGCCCCGAGAGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGa    >  1:462692/3‑150 (MQ=255)
                                                                                                                                          aaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATg  >  2:174053/1‑150 (MQ=255)
                                                                                                                                          aaaaaTCCGCCTCTCGGGGCGGATTTTTGTTTTTAACGTTTCGGGTCGAAAATATCGGTTCCGAGAGGGTTATAATCAACCTTATGTAACTGGAAGTTGGTGATGTAAATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTAATTGATg  >  1:715898/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                          
ACGGTGCGGGTCTGCAGTTCAACCCGATGGAAAACGTTGCTCTGGACTTCTCTTACGAGCAGAGCCGTATTCGTAGCGTTGACGTAGGCACCTGGATTGCCGGTGTTGGTTACCGCTTCTAATCACTTTGGTGATATAAAAAATCCGCCTCTCGGGGCGGATTTTTGTTTTTAAGGTTTCGGGTCGAAAATATCGGTTCCGAGATGGTTGTAATCCACCTTCTGTAACTGGAAGTTGGTGATGTATATCGGCCCGGCTTTCTTCTCAGATATAAACGGGTATTTGATG  >  NC_000913/850844‑851131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑