Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 85,338 A→C 6.0% intergenic (+26/‑292) leuO → / → ilvI global transcription factor/acetolactate synthase 3 large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091385,3380AC 6.0% 243.0 / 4.3 83intergenic (+26/‑292)leuO/ilvIglobal transcription factor/acetolactate synthase 3 large subunit
Reads supporting (aligned to +/- strand):  ref base A (42/36);  new base C (3/2);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

GGTATTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCTGTAA  >  NC_000913/85189‑85485
                                                                                                                                                     |                                                                                                                                                   
ggTATTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGa                                                                                                                                                     >  1:1002695/1‑150 (MQ=255)
   aTTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGATGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATg                                                                                                                                                  >  1:128430/1‑150 (MQ=255)
   aTTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATg                                                                                                                                                  <  1:528188/150‑1 (MQ=255)
      aCCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCt                                                                                                                                               >  2:460279/1‑150 (MQ=255)
       ccGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCATAATAGGTCAGACATGAATGTCTg                                                                                                                                              >  2:346685/1‑150 (MQ=255)
         gCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGt                                                                                                                                            <  2:1245322/150‑1 (MQ=255)
          cTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGtt                                                                                                                                           <  1:410134/150‑1 (MQ=255)
          cTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGtt                                                                                                                                           <  1:1194086/150‑1 (MQ=255)
          cTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCAAGAAGCTGCCGGGCGCGATAATGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGtt                                                                                                                                           >  2:236498/1‑150 (MQ=255)
               gTTAAAACAAAACAGCAGTACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTc                                                                                                                                      <  1:1214781/150‑1 (MQ=255)
               gTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTAttg                                                                                                                                      >  2:516381/1‑149 (MQ=255)
                 tAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTg                                                                                                                                    <  2:583851/150‑1 (MQ=255)
                  aaaacaaaacAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAAACCAGACATTCATGTCTGACCTATTCTGc                                                                                                                                   <  2:993213/150‑1 (MQ=38)
                   aaacaaaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCa                                                                                                                                  >  2:1255909/1‑150 (MQ=255)
                   aaacaaaacaGCAGAACCTGTTATCTCCCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCa                                                                                                                                  <  1:362401/150‑1 (MQ=255)
                      caaaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGATTGTCTGGTTTATTCTGCAttt                                                                                                                               >  1:75098/1‑150 (MQ=255)
                      caaaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAttt                                                                                                                               <  1:314733/150‑1 (MQ=255)
                         aacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAtttttt                                                                                                                            <  2:950306/150‑1 (MQ=255)
                          acaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTa                                                                                                                           <  2:29402/150‑1 (MQ=255)
                               agAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAAACATGAATGTCTGGTTTATTCTGCATTTTTTATTGaa                                                                                                                      >  2:3343/1‑150 (MQ=255)
                                gAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAGTTTTTATTGAAt                                                                                                                     >  2:1010781/1‑150 (MQ=255)
                                 aaCCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATg                                                                                                                    <  1:343479/150‑1 (MQ=255)
                                             ctcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTAtt                                                                                                        >  1:637948/1‑150 (MQ=255)
                                             ctcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTAtt                                                                                                        >  2:123329/1‑150 (MQ=255)
                                             ctcCTGGCATGAAGCTGACGGGAGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTAtt                                                                                                        <  2:765268/150‑1 (MQ=255)
                                              tcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTc                                                                                                       <  2:643431/150‑1 (MQ=255)
                                               ccTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCt                                                                                                      <  1:123329/150‑1 (MQ=255)
                                                   gCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAAt                                                                                                  >  2:1168158/1‑150 (MQ=255)
                                                    cATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAACCGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATGCTGCATTTTTTATTGAATGTAGAATTTTATTATGAAtt                                                                                                 >  2:630388/1‑149 (MQ=255)
                                                    cATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAAtt                                                                                                             >  1:299484/1‑138 (MQ=255)
                                                    cATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAAtt                                                                                                             <  2:299484/138‑1 (MQ=255)
                                                     aTGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAAtgt                                                                                                >  2:27642/1‑150 (MQ=255)
                                                      tGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAAtgtg                                                                                               >  1:666987/1‑150 (MQ=255)
                                                       gAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAAtgtgt                                                                                              >  1:20180/1‑150 (MQ=255)
                                                         aGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTgg                                                                                            <  1:856459/150‑1 (MQ=255)
                                                         aGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTgg                                                                                            <  1:253763/150‑1 (MQ=255)
                                                          gCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCAAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTggg                                                                                           <  2:1099789/150‑1 (MQ=255)
                                                          gCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAAATTGCAAACGCTAACTGATTGCAGAAAAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTAATTCTGAATGTGTggg                                                                                           >  2:449962/1‑150 (MQ=255)
                                                                  gcgcGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTATCATTTTGCATACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTAt                                                                                   <  1:1126877/150‑1 (MQ=255)
                                                                     cgATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCATACGCTAACTGATTGCAGAATAGGGCAGACATGAATGTCTGGATTATTGTGCATTTTTTATTGATTGTTGAATTTTTTTGTGATTGTGTGGGCTCTCTAtttt                                                                                >  2:927707/1‑150 (MQ=255)
                                                                      gATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTa                                                                               >  1:261911/1‑150 (MQ=255)
                                                                       aTAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAg                                                                              >  2:1142126/1‑150 (MQ=255)
                                                                              ccATCAGTGGATGGAATAGCAATTAGTCTCAATTTGCAAACGCTACCGGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGattaat                                                                       <  2:127112/150‑1 (MQ=255)
                                                                              ccATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGattaat                                                                       <  1:741952/150‑1 (MQ=255)
                                                                              ccATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGattaat                                                                       >  1:756609/1‑150 (MQ=255)
                                                                                aTCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGattaatta                                                                     >  2:127558/1‑150 (MQ=255)
                                                                                      ggatggaAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTaaaaaaa                                                               >  2:986738/1‑150 (MQ=255)
                                                                                          ggaAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATaga                                                           <  2:1015153/150‑1 (MQ=255)
                                                                                             agagCAATTAGTCTCAATATGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGaa                                                        <  1:1190827/150‑1 (MQ=255)
                                                                                             agagCAATAATTCTCCAATTGCAATCGCTAACTGATTGCAGAATAGTGCAGAGATCATTGTCTGGTTTAATCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGaa                                                        <  1:1190386/150‑1 (MQ=255)
                                                                                              gagCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGaaa                                                       <  1:370411/150‑1 (MQ=255)
                                                                                               agCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAAt                                                      >  2:724746/1‑150 (MQ=255)
                                                                                                gCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAAtt                                                     >  1:1236538/1‑150 (MQ=255)
                                                                                                  aaTTAGTCTTAATTTGAAAACGCTAACTGATTGCAGAATATGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTga                                                   <  1:346685/150‑2 (MQ=255)
                                                                                                   aTTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCt                                                  <  2:400922/150‑1 (MQ=255)
                                                                                                       gTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTaa                                              <  1:1255909/150‑1 (MQ=255)
                                                                                                           aaaTTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTtg                                          <  2:1044793/149‑1 (MQ=255)
                                                                                                               ttGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTTGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTggg                                      <  2:388951/150‑1 (MQ=255)
                                                                                                                 gCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTATGTTGTGGGAt                                    >  1:279464/1‑150 (MQ=255)
                                                                                                                 gCAAAAGCTAACTGATTGCATAATAGGTCAGACATGAATGTCTGGTTTATTATGCATTTTTTATTTAATGTAGAATTTTATTCTGAATTTTTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGATAAATTGCTGTAAGTTTTTGGAt                                    >  2:930714/1‑150 (MQ=255)
                                                                                                                      cGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGc                               >  1:908935/1‑150 (MQ=255)
                                                                                                                      cGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGc                               <  1:266591/150‑1 (MQ=255)
                                                                                                                       gCTAACTGATTGCAGAATAGGTCAGACATTAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGATTGTGTGGGCTGTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGcc                              >  2:794794/1‑150 (MQ=255)
                                                                                                                       gCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGcc                              <  1:765223/150‑1 (MQ=255)
                                                                                                                        cTAACTGATTGCAGAATAGGTCAGCCATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTTTTTTAGGTTTTATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCg                             >  2:676266/1‑150 (MQ=255)
                                                                                                                        cTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCg                             >  1:132401/1‑150 (MQ=255)
                                                                                                                           aCTGATTGCAGAATAGGTCAGACATGAATGTCTGTTTTATTCTTCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGAtt                          <  1:66526/150‑1 (MQ=255)
                                                                                                                             tGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAATAATAGAGAAATTGCTGTAAGTTGTGGGATTAAGCCGATtta                        >  1:903747/1‑150 (MQ=255)
                                                                                                                               aatGCAGAATAAACCAGACATTCATGTCTGACCTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATttatt                      >  1:687068/3‑150 (MQ=38)
                                                                                                                               aatGCAGAATAAACCAGACATTCATGTCTGACCTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATttatt                      >  1:808645/3‑150 (MQ=38)
                                                                                                                                  gCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTTAATGTAGATTTTTATTCTGAATGTTTGGGATCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATc                   >  2:499684/1‑150 (MQ=255)
                                                                                                                                  gCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTtattata                   >  2:1210226/1‑149 (MQ=255)
                                                                                                                                     gAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAAt                >  1:742040/1‑150 (MQ=255)
                                                                                                                                     gAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGATGTAAGTTTTGGGTTTCAGCCGATTTATTATCAAt                >  1:813792/1‑150 (MQ=255)
                                                                                                                                       ataaaccAGACATTCATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAAttt              >  2:688883/7‑150 (MQ=255)
                                                                                                                                         aGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTaa            >  2:509428/1‑150 (MQ=255)
                                                                                                                                          ggTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAAt           >  2:446347/1‑150 (MQ=255)
                                                                                                                                          ggTCAGACATGAAAGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAAt           <  1:254712/150‑1 (MQ=255)
                                                                                                                                           ggcAGACATGAATGTCTGGTTTATTCTGCATTTTTTTTTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATc          <  2:560144/148‑1 (MQ=255)
                                                                                                                                           gTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAATAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTTTCAATTTAATc          >  2:169235/1‑150 (MQ=255)
                                                                                                                                            ccAGACATTCATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATcc         <  1:688883/149‑1 (MQ=255)
                                                                                                                                              aGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCctc       <  2:824970/150‑1 (MQ=255)
                                                                                                                                               gACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATActct      >  1:656811/1‑150 (MQ=255)
                                                                                                                                                   tGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTATGATTAATTAAAAAAATATATATATTTATGTAAGTTGTGGAATTCAGCCGATTTATTATCAATTTAATCCTCTGTaa  >  1:172379/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGTATTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCTGTAA  >  NC_000913/85189‑85485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: