Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 940,754 G→A 6.1% intergenic (+34/‑205) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913940,7540GA 6.1% 211.6 / 3.0 82intergenic (+34/‑205)serS/dmsAseryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base G (33/43);  new base A (2/3);  total (36/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

TGGCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAAT  >  NC_000913/940605‑940903
                                                                                                                                                     |                                                                                                                                                     
tggCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCg                                                                                                                                                       >  1:134649/1‑150 (MQ=255)
  gCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCggg                                                                                                                                                     <  1:28652/150‑1 (MQ=255)
  gCTGGTGGTCTTACGCTGGTTGCAGAAATGGAAAACTATGAGCATGCTGATTGTCGTCTTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATGGGCTAATACCCAATATTGCTGAATCTAAAAAGCGCCTGCggg                                                                                                                                                     <  1:209886/150‑1 (MQ=255)
     gTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGgcgc                                                                                                                                                  >  2:242454/1‑150 (MQ=255)
     gTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGgcgc                                                                                                                                                  <  1:437916/150‑1 (MQ=255)
            gTACGCGGGTTGCAGTAATGGAAACCTAGCAGCCGGCTGATGGTCGTATTGAAGAACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTACTACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttag                                                                                                                                           <  2:160804/150‑3 (MQ=255)
             tACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTg                                                                                                                                          >  2:519550/1‑150 (MQ=255)
             tACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTg                                                                                                                                          >  2:929067/1‑150 (MQ=255)
             tACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttaga                                                                                                                                          <  2:114408/150‑4 (MQ=255)
             tACGCTGGTTGCAGGAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGCTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTg                                                                                                                                          <  1:345813/150‑1 (MQ=255)
              aCGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGAATTGAAGTACCAGAACTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttagat                                                                                                                                         <  1:307766/150‑5 (MQ=255)
                       gCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTg                                                                                                                                <  1:389649/150‑1 (MQ=255)
                         aGTAATGGAAAACTATCAGGAGGCTGATGGTCGTATTGAAGTAACAGAAGTTCTGCGTGCGTTTATGATGGGACTGGAATATATTGGCTAATACCCATTTTTTCTGAATCTAAATAGCGCCTGCGGGCGCTTTATTTGTCTCCCTATGAt                                                                                                                              >  1:757821/1‑150 (MQ=255)
                         aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCTGGCGCTTTTTTTGTCTCCCTTTGAt                                                                                                                              >  1:801373/1‑150 (MQ=255)
                         aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGAt                                                                                                                              <  1:413478/150‑1 (MQ=255)
                         aGTAATGGAAAACTATCAGCAGGCTGATGCGCGTATTGAAGTAACAGAAGTTCTGCGTCCGAATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTATGAt                                                                                                                              >  1:752574/1‑150 (MQ=255)
                         aGTAATGGAAAACTATCAGAAGGCTGATGGTCGTATTGAAGTAACAGAAGTTCTGAGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAATCTCCTGCGGGAGCTTTTTTTGTATACCTTTGAt                                                                                                                              >  1:752572/1‑150 (MQ=255)
                            aaTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATAcc                                                                                                                           <  2:762422/150‑1 (MQ=255)
                            aaTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATAcc                                                                                                                           <  1:857136/150‑1 (MQ=255)
                            aaTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATAcc                                                                                                                           <  1:834850/150‑1 (MQ=255)
                             aTGGAAAACTATCAGCAGGCTGTTGGGCGTATTGAAGTACCAGATGTTCAGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGGGCTTTTTTTGTCTCCCTTTGATACCg                                                                                                                          <  2:597844/150‑1 (MQ=255)
                              tGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGa                                                                                                                         >  1:7262/1‑150 (MQ=255)
                                    aCTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACaata                                                                                                                   >  2:269120/1‑150 (MQ=255)
                                     cTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACaataa                                                                                                                  <  1:692498/150‑1 (MQ=255)
                                          agcagGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATGTTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTAct                                                                                                             <  1:731549/150‑1 (MQ=255)
                                          agcagGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTAct                                                                                                             >  1:620887/1‑150 (MQ=255)
                                            cagGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActcc                                                                                                           <  2:1209362/150‑1 (MQ=255)
                                              ggCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCTCTTTTTTTTTCTCCCTTTGATACAGAACAATAATTactacta                                                                                                         >  1:646165/1‑149 (MQ=255)
                                              ggCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActcctc                                                                                                         >  1:680738/1‑150 (MQ=255)
                                                      gTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTACCTTTGATAACGAACAATAATTACTCCTCACTTacac                                                                                                 >  1:182617/1‑150 (MQ=255)
                                                         gTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTa                                                                                              <  2:893972/150‑1 (MQ=255)
                                                             tGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAtac                                                                                          <  1:39337/150‑1 (MQ=255)
                                                                aGTACGAGTAGTTCTTCGACCGTATATGATCGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAtactac                                                                                       <  2:204180/150‑1 (MQ=255)
                                                                aGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAtactac                                                                                       <  2:66410/150‑1 (MQ=255)
                                                                  tACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACtt                                                                                     >  1:1127099/1‑150 (MQ=255)
                                                                   aCCAGAAGTTCTGCGTCCGTATATGACCGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGAGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATGACTCCTCACTTACACGTAATAGTTCttt                                                                                    >  1:79235/1‑150 (MQ=255)
                                                                   aCCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACttt                                                                                    >  2:1018633/1‑150 (MQ=255)
                                                                     cAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGATTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCg                                                                                  >  1:1162643/1‑150 (MQ=255)
                                                                         aGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTg                                                                              <  2:396340/150‑1 (MQ=255)
                                                                          gTTCTGCGTCCGTATATGAACGGACTGGAATATATTTGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATAACGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGa                                                                             >  1:262624/1‑150 (MQ=255)
                                                                          gTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGa                                                                             >  1:240588/1‑150 (MQ=255)
                                                                                cGTCCGTATATAAACGGACTGGAATATATTGGATAATACCCAATATTTCTTAATCTAAAAAGCGCCTGCAGTAGCTTTTTTTATCTCCATTTGATACCGAACAATAATTACTCCTCACTTACAAGTAATAATACTTTCGAGTGAAAATCt                                                                       >  1:714028/1‑150 (MQ=255)
                                                                                   ccGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTAcc                                                                    <  2:1045873/150‑1 (MQ=255)
                                                                                   ccGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTAcc                                                                    <  1:1060879/150‑1 (MQ=255)
                                                                                    cGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCt                                                                   >  2:1292494/1‑150 (MQ=255)
                                                                                     gTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTa                                                                  <  1:229795/150‑1 (MQ=255)
                                                                                      tataTGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTAt                                                                 >  1:399066/1‑150 (MQ=255)
                                                                                       atatGAACGTACTGTAATATTTTGGCTAATAGCCAATTATTCTGTATCTAAAAAGCTCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTAtc                                                                <  2:63767/150‑1 (MQ=255)
                                                                                       atatGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTAtc                                                                <  2:1206300/150‑1 (MQ=255)
                                                                                        tatGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTAtct                                                               <  1:1259104/150‑1 (MQ=255)
                                                                                         atGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTAtctc                                                              <  1:651863/150‑1 (MQ=255)
                                                                                          tGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATctct                                                             >  1:3771/1‑150 (MQ=255)
                                                                                          agAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTAACACGTAATACTACTTTCGAGTGAAAATCTACCTATctct                                                             >  1:240899/2‑150 (MQ=255)
                                                                                            aaCGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCttt                                                           <  1:343887/150‑1 (MQ=255)
                                                                                                   tGGAATATTTTGGCTAATAGCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTc                                                    <  2:583757/150‑1 (MQ=255)
                                                                                                   tGGAATATATTGGCTAATACCAAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTc                                                    <  2:947177/150‑1 (MQ=255)
                                                                                                   tGGAATATATTGGCTAATACACAATTTTTCTTAATCTAAATAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCTAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTc                                                    <  1:507390/150‑1 (MQ=255)
                                                                                                    ggAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCa                                                   <  2:620887/150‑1 (MQ=255)
                                                                                                       atatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAat                                                <  2:250100/150‑1 (MQ=255)
                                                                                                       atatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAAAAATAATTACTCCTCACGTACACGTAATACTACTTTAGAGTGAAAATCTACCTATCTCTTTGATTTTCAAat                                                >  1:395699/1‑150 (MQ=255)
                                                                                                           atTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCGTTTTTTGTCTCCCTTTGATACCGAACAATAATTACAACTCCCTTACACGTAATTCTACTTTCGAGTGAAAATCTAACTATCTCTTTGATTTTAAAattatt                                            >  1:1088265/1‑150 (MQ=255)
                                                                                                           atTGGCTAATACAAAATTTTTCTGAATATAAAAATCTACTGAGGTCGATTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACTCGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAattatt                                            <  1:565752/150‑1 (MQ=255)
                                                                                                                cTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATg                                       <  1:1044923/150‑1 (MQ=255)
                                                                                                                cTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATg                                       <  2:12624/150‑1 (MQ=255)
                                                                                                                cTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGAGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCCCTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTTATTTTCAAATTATTCGAtt                                       >  1:62452/1‑149 (MQ=255)
                                                                                                                  aaTACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGta                                     <  1:815740/150‑1 (MQ=255)
                                                                                                                      cccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTACTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTAGATGTATACa                                 >  2:1014600/1‑150 (MQ=255)
                                                                                                                        cAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAg                               >  2:174894/1‑150 (MQ=255)
                                                                                                                        cAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAg                               <  1:1288238/150‑1 (MQ=255)
                                                                                                                           tttttCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCt                            <  2:981923/150‑1 (MQ=255)
                                                                                                                           tttttCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCt                            <  2:983317/150‑1 (MQ=255)
                                                                                                                              ttCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAAACTACTTTCGAGTGAAAATCTACCTATCTCTTAGATTTTCAAATTATTCGATGTATACAAGCCtatt                         >  2:331041/1‑149 (MQ=255)
                                                                                                                               tCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTGTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTAGAGTGAAAATATACCTATATCTTTGATTTTCAAATTATTAGATGTATAAAAGCCtataa                        >  2:1092075/1‑149 (MQ=255)
                                                                                                                               tCTGAATCTAAAAAGCGCCGGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtatat                        <  2:1169309/150‑1 (MQ=255)
                                                                                                                                  gAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGc                     <  2:1110991/150‑1 (MQ=255)
                                                                                                                                   aaTCTAAAAAGCGACTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATTCTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATTTAGCg                    <  1:458431/150‑1 (MQ=255)
                                                                                                                                       tAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTGTCGAGTGAAAATCTACCTATCACTTTGAATTACAAATTTTTCGATGTATACAAGCCTATATATCGaaat                >  2:56762/1‑148 (MQ=255)
                                                                                                                                       tAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCATTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACATATCTCTTTGATTTTCATATTATTCGATGTATACAAGCCTATATAGCGAACt                >  2:924024/1‑150 (MQ=255)
                                                                                                                                         aaaaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCCAACAATAATTACACCTCACTTACACGTAATACTACTTTCGAGGGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc              <  2:174014/150‑1 (MQ=255)
                                                                                                                                          aaaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCt             <  1:425032/150‑1 (MQ=255)
                                                                                                                                          aaaGCGCCCGCAGGCGCTTTTTTTGTGTCCCTTTGATACCGAACAATAAGTACTCCTCACTTACACGTAAAACTACTTTCGAGTTAAAATCTACCTATCTCTATGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAAcagat             >  1:327557/1‑146 (MQ=255)
                                                                                                                                              cgcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAg         >  1:368410/1‑150 (MQ=255)
                                                                                                                                               gcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGa        <  2:680738/150‑1 (MQ=255)
                                                                                                                                                ccTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGCAAAATAATTACTCCTCACTTACACGGAATTCTACTTTCGAGTGAAAATCTACCGATCTCTTTGATTTTCAAATTATTGGATGTATAAAAGCCTATATAGCGCACTGCTATAGaa       >  2:1124878/1‑150 (MQ=255)
                                                                                                                                                   gCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaata    <  2:1127099/150‑1 (MQ=255)
                                                                                                                                                     gggCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataat  >  2:344122/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAAT  >  NC_000913/940605‑940903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: