Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 993,775 A→C 8.0% R90R (CGT→CGG ssuB ← aliphatic sulfonate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,7750AC 8.0% 194.5 / 2.4 74R90R (CGT→CGGssuBaliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (31/36);  new base C (5/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGTTTCTGCCCGCCAGAAAGTGCGGCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGC  >  NC_000913/993626‑993924
                                                                                                                                                     |                                                                                                                                                     
cGCTGTTTCTGCCCGCCAGAAAGTGCGGCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGa                                                                                                                                                       >  2:1308704/1‑150 (MQ=255)
  gtGTTTCTGGCCGCGAGAATGTGCGGCAGGCAATTCAGATTCGCGATTCTCCAGTCCTACCGCAGCCAAAGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGAcg                                                                                                                                                     <  2:961393/149‑1 (MQ=255)
        cTGCCCGCCAGAAAGTGCGGCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTCTCAATCACCGATTTCCCCGCTAGCAGACGCGCATc                                                                                                                                               >  1:180003/1‑150 (MQ=255)
         tGCCCGCCAGAAAGTGCGGCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATcc                                                                                                                                              <  2:497016/150‑1 (MQ=255)
           cccGCCAGAAAGTGCGGCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTg                                                                                                                                            >  1:282253/1‑150 (MQ=255)
             cGCCAGAAAGTGCGGCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGaa                                                                                                                                          >  1:302878/1‑150 (MQ=255)
               ccAGAAAGTGCGGCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAAc                                                                                                                                        <  1:676417/150‑1 (MQ=255)
               ccAGAAAGTGCGGCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAAc                                                                                                                                        >  1:140487/1‑150 (MQ=255)
                         cGGCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTcgcg                                                                                                                              <  2:1090793/150‑1 (MQ=255)
                         cGGCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATAATTcgct                                                                                                                              >  2:210614/1‑149 (MQ=255)
                           gCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCgtg                                                                                                                            <  2:1018059/150‑1 (MQ=255)
                               gCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGCCGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATGTCCACGGTAGCAGCCGCGCATCCTGATACATCATTCGCGTGTCtt                                                                                                                        >  2:915431/1‑150 (MQ=255)
                                  aTTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCt                                                                                                                     >  2:1050726/1‑150 (MQ=255)
                                   ttCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTg                                                                                                                    <  1:255267/150‑1 (MQ=255)
                                     ccccTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCATGAAACATCATTCGCGTGTCTTCCTGaa                                                                                                                  >  1:830545/1‑150 (MQ=255)
                                         cgcgcGTTTCTCCAGTCCTACCGCAGCCAAGCCGCGACGTGCGGCATCGCGCCACTGGCCTATAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTc                                                                                                              <  2:220288/149‑1 (MQ=255)
                                          gcgcGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTca                                                                                                             <  1:1050726/150‑1 (MQ=255)
                                           cgcgATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTcag                                                                                                            <  1:170728/150‑1 (MQ=255)
                                           cgcgATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTcag                                                                                                            <  1:331914/150‑1 (MQ=255)
                                             cgATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAAGTTcagcc                                                                                                          >  1:182496/1‑150 (MQ=255)
                                                 tctcCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCATAACCCAACGTTATCAACCACCGATTTCCCCGGTAGCAGCCGCGCCTCCTGAACCATCCTTCGCGTGTCTGCCTGCAGTTCAGCCcgcg                                                                                                      >  1:908285/1‑150 (MQ=255)
                                                   tcCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAGCCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAACCATCCTTCGCGTGTCTTCCTGAATTTCAGCCAGCGgt                                                                                                    >  2:842223/1‑150 (MQ=255)
                                                    ccAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCACTCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATGCGCGTGTCTGCCTGAATTTCAGCCAGCGgtg                                                                                                   >  2:1064676/1‑150 (MQ=255)
                                                    ccAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGAGTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAAGTTCAGCCAGCGgtg                                                                                                   >  1:1202225/1‑150 (MQ=255)
                                                            aCCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCCCGGTAGCAGACGCGCATCCTGAAACCTCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGccc                                                                                           >  1:514074/1‑150 (MQ=255)
                                                            aCCGCAGCCAACGCTCGACGTGCGGCATCGCGCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCg                                                                                          <  2:904644/150‑1 (MQ=255)
                                                            aCCGCAGCCAACGCGCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGCTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGGCTTCCTGAATTTCAGCCAGCGGTGTGGTGccc                                                                                           <  2:243027/150‑1 (MQ=255)
                                                               gCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCt                                                                                        >  2:766503/1‑150 (MQ=255)
                                                                 aGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAac                                                                                     <  2:1172695/150‑1 (MQ=255)
                                                                   ccAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGAGGTGTGGTGCCCGCTAaca                                                                                    >  2:1132177/1‑150 (MQ=255)
                                                                    cAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAacac                                                                                   >  2:150632/1‑150 (MQ=255)
                                                                     aaCGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAAcaca                                                                                  <  2:172589/150‑1 (MQ=255)
                                                                            gACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGAATTCCACGGTAGCATACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCCGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTg                                                                           >  1:859498/1‑150 (MQ=255)
                                                                                 gCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGtt                                                                      >  1:436420/1‑150 (MQ=255)
                                                                                   ggCATCGCGCCACTGGCCTTTAAGGCCCAACCCAACGTTATCAATCACCGATTTCCACGGGAGCAGCCGCGCATCCTGAAACATCATTCGCGTGGCTTCCTGAATTTCAGCCAGCGGGGTGGTGCCCGCTAACACATCGCCTGCGGGTgg                                                                    <  2:78635/150‑1 (MQ=255)
                                                                                    gCATAGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCACGCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCTGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGc                                                                   <  2:192090/150‑1 (MQ=255)
                                                                                     cATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCg                                                                  <  2:541766/150‑1 (MQ=255)
                                                                                     cATCGCGCCACTGGCCGTTAAGGCCTAACCCAACGTTATCAATCACCGATGTCCACGGTAGCAGCCGCGCATCCTGAAACATCATTCGCGTGTCTGCCTGAATTTCAGCCAGCGCTGTGGTGCCCGCTAACACATCGCCTGCGGTGGGCg                                                                  >  1:70561/1‑150 (MQ=255)
                                                                                       tCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGtt                                                                <  2:1107043/150‑1 (MQ=255)
                                                                                          cgcCACTGGCCTTTACGGCCTAACCCAACGTTATCAATCACCGATTGCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTcc                                                             <  2:212840/150‑1 (MQ=255)
                                                                                          cgcCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTcc                                                             <  2:140487/150‑1 (MQ=255)
                                                                                          cgcCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTcc                                                             <  1:944266/150‑1 (MQ=255)
                                                                                           gcCACTGGCCTTTAAGGCATAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGCCGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAAGTTCAGCCAGCGGTGTGGTGCCCGCTAACCCATCGCATGCGGTTGGCGTTTCCa                                                            >  1:802641/1‑150 (MQ=255)
                                                                                            ggttaggCCTTAAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAg                                                           >  1:948869/6‑150 (MQ=255)
                                                                                             cACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAgc                                                          >  2:408370/1‑149 (MQ=255)
                                                                                              aCTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGAc                                                         <  2:572301/150‑1 (MQ=255)
                                                                                               cTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGAcc                                                        >  2:457551/1‑150 (MQ=255)
                                                                                                   ccgttAAGGCCTAACCCAACGGTATCAATCACCGATATCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGCGTGGGGCCCGCTAACCCATCGCCTGCGGTTGGCGTTTCCAGACCTGcc                                                    <  2:472747/147‑1 (MQ=255)
                                                                                                   ccTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGCCGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCCGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGcc                                                    >  2:183748/1‑150 (MQ=255)
                                                                                                   ccTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGcc                                                    <  2:454476/150‑1 (MQ=255)
                                                                                                        aaGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTTACACATCGCCTGCGGTTGGCGTTTCCAGCCCTGCcagcag                                               >  2:170538/1‑150 (MQ=255)
                                                                                                          ggCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGgc                                             >  2:510787/1‑150 (MQ=255)
                                                                                                             cTAACCCAACCGTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGAGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCgca                                          <  2:851547/150‑1 (MQ=255)
                                                                                                                aCCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCgcagca                                       >  2:101605/1‑150 (MQ=255)
                                                                                                                aCCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCgcagca                                       <  2:46536/150‑1 (MQ=255)
                                                                                                                  ccAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAgg                                     >  1:568287/1‑150 (MQ=255)
                                                                                                                   cAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAggg                                    <  2:681261/150‑1 (MQ=255)
                                                                                                                   cAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAggg                                    <  2:1191133/150‑1 (MQ=255)
                                                                                                                   cAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAggg                                    >  1:1265046/1‑150 (MQ=255)
                                                                                                                   cAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAggg                                    <  2:282253/150‑1 (MQ=255)
                                                                                                                     aCGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTa                                  >  1:1142538/1‑150 (MQ=255)
                                                                                                                      cGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTAAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGGAc                                 >  1:443100/1‑150 (MQ=255)
                                                                                                                         tATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACttt                              >  1:874037/1‑150 (MQ=255)
                                                                                                                         aatcaatcaCCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACttt                              <  1:15250/149‑1 (MQ=255)
                                                                                                                             aatcaCCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGcc                          <  1:1086996/150‑1 (MQ=255)
                                                                                                                                 aCCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATGTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGccacca                      <  2:1185958/150‑1 (MQ=255)
                                                                                                                                  ccGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCcaccac                     <  2:1199245/150‑1 (MQ=255)
                                                                                                                                   cGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCaccacc                    <  2:1262399/150‑1 (MQ=255)
                                                                                                                                     aTTTCCACGGTAGCAGACGCGCATCCTGACACATCATTCGCGTGGCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGc                  <  1:915431/150‑1 (MQ=255)
                                                                                                                                      tttCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCt                 <  1:1123675/150‑1 (MQ=255)
                                                                                                                                           aCGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGc            >  1:415163/1‑150 (MQ=255)
                                                                                                                                             ggTAGCAGCCGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGcct          <  1:210614/150‑2 (MQ=255)
                                                                                                                                             ggTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGccc          <  2:792868/150‑1 (MQ=255)
                                                                                                                                               tAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCccac        >  1:902077/1‑150 (MQ=255)
                                                                                                                                                 gCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCCTTTCCAGACCTGCCAGCAGGCGCCGCAGGGTACTTTTGCCACCACCGCTCCGGccccccc      >  1:242154/1‑149 (MQ=255)
                                                                                                                                                     aCGCGCATCCTGCAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGc  <  1:244979/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCTGTTTCTGCCCGCCAGAAAGTGCGGCAGGCCATTCCCCTGCGCGATTCTCCAGTCCTACCGCAGCCAACGCTCGACGTGCGGCATCGCGCCACTGGCCTTTAAGGCCTAACCCAACGTTATCAATCACCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGC  >  NC_000913/993626‑993924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: