Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 87,881 A→G 6.5% intergenic (+33/‑147) ilvH → / → cra acetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091387,8810AG 6.5% 223.0 / 5.9 77intergenic (+33/‑147)ilvH/craacetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism
Reads supporting (aligned to +/- strand):  ref base A (42/30);  new base G (2/3);  total (44/33)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

GGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTG  >  NC_000913/87732‑88030
                                                                                                                                                     |                                                                                                                                                     
ggCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACa                                                                                                                                                       <  2:1046286/150‑1 (MQ=255)
      aGCGGTAAGCTTGATGCATTTTTAGTATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGAGCGGCGAATAATAAATGGGTTGAGATTTCTCTCAAGGCGCAAATTACTTCACAACATTTCAc                                                                                                                                                 >  2:52482/1‑150 (MQ=255)
       gCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACg                                                                                                                                                >  2:1092689/1‑150 (MQ=255)
        cGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGt                                                                                                                                               <  1:491130/150‑1 (MQ=255)
           tAAGCGTGATGCATTTTTAGCATCGATTCGCGATGGGGCGAAAATTGGGGAGGTTGCTCGCTCTGGTGTGGGCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg                                                                                                                                            <  2:613388/150‑1 (MQ=255)
               cTTGATGCATTTTTATCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtg                                                                                                                                        <  1:802564/150‑2 (MQ=255)
               cTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtg                                                                                                                                        <  2:1054366/150‑2 (MQ=255)
               cTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtt                                                                                                                                        <  2:855636/150‑1 (MQ=255)
                ttGATGCATTTTTAGCATCGATTCTCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGTGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtgt                                                                                                                                       <  1:749939/150‑3 (MQ=255)
                     gCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTg                                                                                                                                  <  1:294803/150‑1 (MQ=255)
                      cATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGATGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCTTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGc                                                                                                                                 <  2:778953/150‑1 (MQ=255)
                       aTTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                                                                <  2:313769/150‑1 (MQ=255)
                       aTTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                                                                <  1:1316447/150‑1 (MQ=255)
                       aTTTTTAGCAGCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGGCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCTAACATGTCACGTTGGGCTTTTTTTGCg                                                                                                                                <  1:654886/150‑1 (MQ=255)
                           ttAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAAt                                                                                                                            >  2:569967/1‑150 (MQ=255)
                            tAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAAta                                                                                                                           >  1:632490/1‑149 (MQ=255)
                            tAGCATAGATTCGCGATGTGGCGAAAATTGTGGAGGTTGATCGCTCTGGTGTGGTCGGACTTTCGAGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAAta                                                                                                                           >  1:987416/1‑149 (MQ=255)
                              gCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAg                                                                                                                         <  2:376002/150‑1 (MQ=255)
                               cATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATc                                                                                                                           >  1:913425/1‑147 (MQ=255)
                               cATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATc                                                                                                                           <  2:913425/147‑1 (MQ=255)
                               cATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGt                                                                                                                        >  2:840832/1‑150 (MQ=255)
                               cATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGt                                                                                                                        >  1:930390/1‑150 (MQ=255)
                                aTCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                                                                       <  2:1023480/150‑1 (MQ=255)
                                 tCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTgg                                                                                                                      <  1:1209244/150‑1 (MQ=255)
                                  cGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTTGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTggg                                                                                                                     <  1:924305/150‑1 (MQ=255)
                                  cGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTggg                                                                                                                     <  1:602692/150‑1 (MQ=255)
                                       cgcgATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCTCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAGCAGGTCACGTTGGGCTTTTTTGGCGAAATCAATGGGATCCt                                                                                                                >  2:208517/1‑150 (MQ=255)
                                                 cGAAAATTGTGGCGGGTGCTCGCTCTGGTGTGCCCGGACTTACGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGATGGGCTTTTCTTGCGAAATCAGTGGGAACCTGGAATAAAAg                                                                                                      <  2:1015648/150‑1 (MQ=255)
                                                     aaTTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGt                                                                                                  <  1:746854/150‑1 (MQ=255)
                                                        tgtgGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGc                                                                                               >  1:637728/1‑150 (MQ=255)
                                                         gtgGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGcc                                                                                              >  1:1072103/1‑150 (MQ=255)
                                                         gtgGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGcc                                                                                              >  2:607825/1‑150 (MQ=255)
                                                              ggTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGGAGt                                                                                         >  1:1256226/1‑150 (MQ=255)
                                                              ggTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGt                                                                                         >  2:1250164/1‑150 (MQ=255)
                                                              ggTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCAAGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGt                                                                                         >  1:799270/1‑150 (MQ=255)
                                                              ggTTGCTCGCTCTGGTGTGGTCGGACTTTAGCGCGCCGATAAAATAAAGCGTTGAGAATGATCTCAATGCGCAATTTACAGACCAACATGTAACGTAGGGCTTTTTTAGCGAAATCAGTGGTAACCTGGAATAAAAGCACTTGCCGCAGt                                                                                         >  1:1129571/1‑150 (MQ=255)
                                                                ttGCTCGCTCTGGTGGGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAATTCAGGGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTa                                                                                       >  1:49774/1‑150 (MQ=255)
                                                                    tcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAAttt                                                                                   >  1:106661/1‑150 (MQ=255)
                                                                       ctctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCt                                                                                >  1:1248662/1‑150 (MQ=255)
                                                                        tctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTg                                                                               >  1:239677/1‑150 (MQ=255)
                                                                           ggTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTTGAATAAAAGCAGTTGCCGCAGTTAATTTTCTgagg                                                                            >  1:304446/1‑147 (MQ=255)
                                                                            gtgtGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCt                                                                           >  2:1311857/1‑150 (MQ=255)
                                                                                   cGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGt                                                                    >  1:808714/1‑150 (MQ=255)
                                                                                   cGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGt                                                                    >  1:515167/1‑150 (MQ=255)
                                                                                    ggACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                                                                   >  2:430457/1‑150 (MQ=255)
                                                                                    ggACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                                                                   >  1:1017184/1‑150 (MQ=255)
                                                                                     gACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTa                                                                  >  1:1224232/1‑150 (MQ=255)
                                                                                       cTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTGGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGGTAATTTTCTGCTCTTATATGtatat                                                                >  1:802935/1‑146 (MQ=255)
                                                                                               cggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTaa                                                        >  2:410081/1‑150 (MQ=255)
                                                                                               cggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTaa                                                        >  1:757415/1‑150 (MQ=255)
                                                                                                ggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGTGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAc                                                       <  2:56644/150‑1 (MQ=255)
                                                                                                 gcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAcc                                                      <  1:376416/150‑1 (MQ=255)
                                                                                                  cgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGTGCTTTTTTTGCGAAATCAGTGGGAACCTGGTATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTTGATGATAATGAATTTAAcac                                                     >  2:246298/1‑148 (MQ=255)
                                                                                                  cgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCAGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAccc                                                     >  2:892262/1‑150 (MQ=255)
                                                                                                  cgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGAAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGATTTTAAccc                                                     >  1:507957/1‑150 (MQ=255)
                                                                                                   gATAAAATAATGCGGTGAGAATGATCTCAATGCGCAATTTCCAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCa                                                    <  2:625910/150‑1 (MQ=255)
                                                                                                     tAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATa                                                  >  2:605095/1‑150 (MQ=255)
                                                                                                        aataatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCa                                               <  1:230833/150‑1 (MQ=255)
                                                                                                           aatGCGTTGAGAATGATCTCAATGCGCAATATACAGCCCAACATGTCACGTTTGGCTTTTTTTGCGAAATCAGTGGGATCCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTAAGCGCTTAGATGTTAATGAATTTAACCCATACCAGTa                                            >  1:651547/1‑150 (MQ=255)
                                                                                                                gTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGAGAAATCAGTGGGAACCTGGAATAAAAGCAGATGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATg                                       >  1:921069/1‑150 (MQ=255)
                                                                                                                     gaATGATCTCAATGCGCAATTTACAGCCAAGCATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGCGCACATGGATTAAAAGCAGTTGCCGCAGTTAATATTGAGCGCTTTGATGTTTATGATATTAACCCATACCAGTACATTGGCttt                                  >  2:90580/1‑148 (MQ=255)
                                                                                                                        tGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGt                               <  1:1205877/150‑1 (MQ=255)
                                                                                                                         gATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGGTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCGTAGATGTTAATGAATTTAACCAATACCAGTACAATGGCTATGGtt                              >  2:145647/1‑150 (MQ=255)
                                                                                                                         gATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTTGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGtt                              <  2:339307/150‑1 (MQ=255)
                                                                                                                          aTCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGttt                             <  1:1092689/150‑1 (MQ=255)
                                                                                                                           tctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGtttt                            <  2:770085/150‑1 (MQ=255)
                                                                                                                            ctcAATGCGCAATTTACAGCCCAACATGTCAGGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCCTACCAGTACATTGGCTATGGttttt                           >  1:868450/1‑150 (MQ=255)
                                                                                                                             tcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTa                          <  1:1046931/150‑1 (MQ=255)
                                                                                                                              cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTAc                         >  2:1259210/1‑150 (MQ=255)
                                                                                                                              aaaTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTAc                         <  1:466423/149‑1 (MQ=255)
                                                                                                                                aTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACAt                       >  1:697910/1‑150 (MQ=255)
                                                                                                                                      aaTTTACATCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACg                 <  2:987416/150‑1 (MQ=255)
                                                                                                                                      aaTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACg                 <  2:632490/150‑1 (MQ=255)
                                                                                                                                         ttACATCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCaa              <  1:1226941/150‑1 (MQ=255)
                                                                                                                                         ttACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTACATTTTACGCAAg             <  2:1305554/150‑1 (MQ=255)
                                                                                                                                            aaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAggg           >  1:1086816/2‑150 (MQ=255)
                                                                                                                                            aaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATAACAGTACAATGGCTATGGTTTTTACATTTTACGCAAggg           >  1:472850/2‑150 (MQ=255)
                                                                                                                                             aGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAgggg          >  1:766758/1‑150 (MQ=255)
                                                                                                                                                     aTGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATtgtg  <  1:840832/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTG  >  NC_000913/87732‑88030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: