Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 998,448 T→A 6.3% intergenic (+41/‑42) elfA → / → elfD laminin‑binding fimbrin subunit/putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913998,4480TA 6.3% 209.3 / 3.3 78intergenic (+41/‑42)elfA/elfDlaminin‑binding fimbrin subunit/putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (37/35);  new base A (2/3);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

GCAGGATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCA  >  NC_000913/998299‑998597
                                                                                                                                                     |                                                                                                                                                     
gCAGGATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGAGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCATCCAgt                                                                                                                                                       >  1:1039337/1‑150 (MQ=255)
   ggATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGtt                                                                                                                                                    >  1:631808/1‑150 (MQ=255)
    gATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGtta                                                                                                                                                   >  1:464148/1‑149 (MQ=255)
     aTGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCt                                                                                                                                                  >  1:708553/1‑150 (MQ=255)
      tGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTg                                                                                                                                                 >  2:299419/1‑150 (MQ=255)
       cgagaTCATACGATGACTTTTAAAGTTGATTATAGCGCTACGGGGAATGCGACTTCATGTAATGTGAATGCGGTACCAAATTTCCATATTACCTCTAATAACTACAACTCATTAATTGTTTTATTAATAAGTACCCCTCCAGTGTTCTgg                                                                                                                                                <  2:630817/149‑1 (MQ=255)
        tagaTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGa                                                                                                                                               <  1:432364/149‑1 (MQ=255)
        gagaTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGa                                                                                                                                               <  1:89707/150‑1 (MQ=255)
           aTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAggg                                                                                                                                            <  1:964351/150‑1 (MQ=255)
            tCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAgggg                                                                                                                                           <  1:125921/150‑1 (MQ=255)
             cAGACGTTGACTTTAAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCATGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAggggt                                                                                                                                          <  2:992279/150‑2 (MQ=255)
               gACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGata                                                                                                                                        <  2:631808/150‑1 (MQ=255)
                aCGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAggggtact                                                                                                                                       <  1:1000968/150‑5 (MQ=255)
                 cGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATAtt                                                                                                                                      >  2:230134/1‑150 (MQ=255)
                  gTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTc                                                                                                                                     <  2:1228896/150‑1 (MQ=255)
                      aCTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCatat                                                                                                                                 >  1:401480/1‑150 (MQ=255)
                       cTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATAtt                                                                                                                                >  2:1033750/1‑150 (MQ=255)
                           tacaGTTGATTATATCTATACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATCtttttt                                                                                                                            <  1:856720/147‑1 (MQ=255)
                            aaaTTTGATTATATCGCTACGGGGAAAGCGACTTCATGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTa                                                                                                                           <  2:1029317/150‑1 (MQ=255)
                              aGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTAAag                                                                                                                         <  1:1074158/150‑1 (MQ=255)
                               gTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAAGGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCACACCAGTGTTCTGGAGGGGATATTCAGATTTTTTaata                                                                                                                        >  1:217072/1‑148 (MQ=255)
                                         tCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTAttt                                                                                                              <  2:767145/150‑1 (MQ=255)
                                          cGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTa                                                                                                             <  2:733773/150‑1 (MQ=255)
                                           gCTACTGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAAGTCATTAATTGTTTTTTTATTTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTAt                                                                                                            <  1:22118/150‑1 (MQ=255)
                                            cTACGGGGAAAGCGACTTCTGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTAtt                                                                                                           >  2:613150/1‑149 (MQ=255)
                                            cTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATg                                                                                                           >  2:1182085/1‑150 (MQ=255)
                                            cTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCAGTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTAtt                                                                                                           >  2:619621/1‑149 (MQ=255)
                                                 gggAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAAc                                                                                                      <  2:782011/150‑1 (MQ=255)
                                                  ggAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACt                                                                                                     <  1:603102/150‑1 (MQ=255)
                                                  ggAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACt                                                                                                     <  1:275873/150‑1 (MQ=255)
                                                     aaGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGc                                                                                                  >  2:828967/1‑150 (MQ=255)
                                                        cGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATa                                                                                               <  2:83381/150‑1 (MQ=255)
                                                        cGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATa                                                                                               <  1:483355/150‑1 (MQ=255)
                                                           cTTCAGGTAATGTGAATGCGGTAACAAATATCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGAATATTTATTAAAACTTGCATAACa                                                                                            >  2:1173296/1‑150 (MQ=255)
                                                              catgTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAg                                                                                         <  2:52237/147‑1 (MQ=255)
                                                              cAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCAAGTGTTCTGGAGGGGATATTCATATTTTTTAATAGTGACTATTTATGAAAACTTGCATAACaaaa                                                                                         >  1:349723/1‑149 (MQ=255)
                                                                ggTAATGTGAATGCTGTAAAAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAggg                                                                                       <  2:203794/150‑1 (MQ=255)
                                                                       tgAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATtgtg                                                                                <  2:1205816/150‑1 (MQ=255)
                                                                       tgAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTGCTGGAGGGGATATTCATATTTTTTAAGATTGACTATTTATGAAAACTTGCATAACAAAGGGATTtgtg                                                                                <  1:796665/150‑1 (MQ=255)
                                                                        gAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGa                                                                               >  2:38776/1‑150 (MQ=255)
                                                                        gAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGa                                                                               >  1:349855/1‑150 (MQ=255)
                                                                          aTGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGAcc                                                                             <  2:243951/150‑1 (MQ=255)
                                                                           tGCTGTAACAAATTTCCATATTACATATTATTAATAGAACTCATTAATTGGTGTATTAATTAGTACCCCTACAGTGTTCGTTATGGGATATTCATATTTTTTAAGAGGGACTATTTCTGAAAACTTTCACAACAAATGGAATTGTGACCg                                                                            <  2:944966/150‑1 (MQ=255)
                                                                                   cAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTa                                                                    >  1:777892/1‑150 (MQ=255)
                                                                                   cAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTa                                                                    >  1:1080872/1‑150 (MQ=255)
                                                                                     aaTTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGTGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAAc                                                                  >  1:790674/1‑150 (MQ=255)
                                                                                     aaTTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTAGCATAACAAAGGGAATTGTGACCGTAAGTTTAAc                                                                  >  2:484257/1‑150 (MQ=255)
                                                                                     aaTTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATATTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAAc                                                                  >  2:123739/1‑150 (MQ=255)
                                                                                           cATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAat                                                            >  2:957413/1‑150 (MQ=255)
                                                                                               ttAACTATTATAAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGATGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTa                                                        <  1:103229/150‑1 (MQ=255)
                                                                                               atAACTATTATTAATACAACTCAATAATTGTTATATTAATTAGTACCCCTCCAGGGTTCAGGAGGGGAAATTCATATTTTTTAAGAGTGACTATTTATGAAAACTAGCATAACAAATGGAAATGAGACCGTAAGTTTAACGGCAATATTa                                                        <  2:74295/149‑1 (MQ=255)
                                                                                                tAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTAc                                                       <  1:264558/150‑1 (MQ=255)
                                                                                                        attaATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAgctgc                                               >  1:1316348/1‑150 (MQ=255)
                                                                                                        attaATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACAGTAAGTTGAACGGCAATATTACTCAgctgc                                               >  2:1024334/1‑150 (MQ=255)
                                                                                                             tAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGATTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCAta                                          >  2:574823/1‑149 (MQ=255)
                                                                                                              aGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATcc                                         <  2:955939/150‑1 (MQ=255)
                                                                                                              aGAACTCATTAATTGATTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAAATGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATcc                                         >  1:437812/1‑150 (MQ=255)
                                                                                                                aaCTCATTAATTGTTTTATTAATTAGTACCCCTCCATTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGc                                       <  1:452095/150‑1 (MQ=255)
                                                                                                                  cTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCTTATTTTTTAAGAGTGACTATTTAGGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCAt                                     >  2:91199/1‑150 (MQ=255)
                                                                                                                    cATTAATTGTTTTATTAAATAGTACCCCTCCTGTGTTCTGGAGGGGATTTTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATgg                                   <  1:833094/150‑1 (MQ=255)
                                                                                                                          ttGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGACATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCa                             <  1:707450/150‑1 (MQ=255)
                                                                                                                           tGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAg                            >  2:936717/1‑150 (MQ=255)
                                                                                                                           tGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAg                            >  2:252779/1‑150 (MQ=255)
                                                                                                                           tGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGAGATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAg                            >  2:754942/1‑150 (MQ=255)
                                                                                                                            gTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAgg                           >  1:747835/1‑150 (MQ=255)
                                                                                                                              tttATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTTACGGCAATATTACTCAGCTGCTCATCCGCATGGGGGGCAGGtt                         >  2:972662/1‑149 (MQ=255)
                                                                                                                                tattaATTAGTACCCCTCCTGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTaaa                       >  2:1113337/1‑150 (MQ=255)
                                                                                                                                 attaattaGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAg                      <  2:1173541/150‑1 (MQ=255)
                                                                                                                                  ttaattaGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAgg                     <  1:429998/150‑1 (MQ=255)
                                                                                                                                        atatccccTCCAGAACACTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAt               >  1:1221093/5‑150 (MQ=255)
                                                                                                                                        aGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAt               >  1:1103195/1‑150 (MQ=255)
                                                                                                                                        aGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAt               <  1:1059980/150‑1 (MQ=255)
                                                                                                                                          tACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTg             >  2:1316379/1‑150 (MQ=255)
                                                                                                                                            ccccTCCAGAACACTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTTAAGGCGGGATTGGa           >  1:1056607/1‑150 (MQ=255)
                                                                                                                                             cccTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGAc          >  1:500114/1‑150 (MQ=255)
                                                                                                                                               cTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACtt        >  2:321824/1‑150 (MQ=255)
                                                                                                                                                tCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTg       <  2:385481/150‑1 (MQ=255)
                                                                                                                                                 ccAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTgc      <  1:1306336/150‑1 (MQ=255)
                                                                                                                                                   aGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTgcag    <  1:230134/150‑1 (MQ=255)
                                                                                                                                                    gtgtTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTgcagc   >  1:446030/1‑150 (MQ=255)
                                                                                                                                                    gtgtTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATAATACTCAGCTGCTCATCCTCATGGGCGGCAGGTTAAGGCGGGATTGTACTTgcagc   >  1:990151/1‑150 (MQ=255)
                                                                                                                                                     tgtTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTgcagca  <  2:349723/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAGGATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCA  >  NC_000913/998299‑998597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: