Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 87,884 T→A 6.3% intergenic (+36/‑144) ilvH → / → cra acetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091387,8840TA 6.3% 215.8 / 4.7 80intergenic (+36/‑144)ilvH/craacetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism
Reads supporting (aligned to +/- strand):  ref base T (44/31);  new base A (2/3);  total (46/34)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.71e-01

AGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGAAA  >  NC_000913/87738‑88033
                                                                                                                                                  |                                                                                                                                                     
aGCGGTAAGCTTGATGCATTTTTAGTATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGAGCGGCGAATAATAAATGGGTTGAGATTTCTCTCAAGGCGCAAATTACTTCACAACATTTCAc                                                                                                                                                    >  2:52482/1‑150 (MQ=255)
 gCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACg                                                                                                                                                   >  2:1092689/1‑150 (MQ=255)
  cGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGt                                                                                                                                                  <  1:491130/150‑1 (MQ=255)
     tAAGCGTGATGCATTTTTAGCATCGATTCGCGATGGGGCGAAAATTGGGGAGGTTGCTCGCTCTGGTGTGGGCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg                                                                                                                                               <  2:613388/150‑1 (MQ=255)
         cTTGATGCATTTTTATCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtg                                                                                                                                           <  1:802564/150‑2 (MQ=255)
         cTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtg                                                                                                                                           <  2:1054366/150‑2 (MQ=255)
         cTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtt                                                                                                                                           <  2:855636/150‑1 (MQ=255)
          ttGATGCATTTTTAGCATCGATTCTCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGTGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtgt                                                                                                                                          <  1:749939/150‑3 (MQ=255)
               gCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTg                                                                                                                                     <  1:294803/150‑1 (MQ=255)
                cATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGATGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCTTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGc                                                                                                                                    <  2:778953/150‑1 (MQ=255)
                 aTTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                                                                   <  2:313769/150‑1 (MQ=255)
                 aTTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                                                                   <  1:1316447/150‑1 (MQ=255)
                 aTTTTTAGCAGCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGGCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCTAACATGTCACGTTGGGCTTTTTTTGCg                                                                                                                                   <  1:654886/150‑1 (MQ=255)
                     ttAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAAt                                                                                                                               >  2:569967/1‑150 (MQ=255)
                      tAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAAta                                                                                                                              >  1:632490/1‑149 (MQ=255)
                      tAGCATAGATTCGCGATGTGGCGAAAATTGTGGAGGTTGATCGCTCTGGTGTGGTCGGACTTTCGAGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAAta                                                                                                                              >  1:987416/1‑149 (MQ=255)
                        gCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAg                                                                                                                            <  2:376002/150‑1 (MQ=255)
                         cATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATc                                                                                                                              >  1:913425/1‑147 (MQ=255)
                         cATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATc                                                                                                                              <  2:913425/147‑1 (MQ=255)
                         cATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGt                                                                                                                           >  2:840832/1‑150 (MQ=255)
                         cATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGt                                                                                                                           >  1:930390/1‑150 (MQ=255)
                          aTCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                                                                          <  2:1023480/150‑1 (MQ=255)
                           tCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTgg                                                                                                                         <  1:1209244/150‑1 (MQ=255)
                            cGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTTGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTggg                                                                                                                        <  1:924305/150‑1 (MQ=255)
                            cGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTggg                                                                                                                        <  1:602692/150‑1 (MQ=255)
                                 cgcgATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCTCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAGCAGGTCACGTTGGGCTTTTTTGGCGAAATCAATGGGATCCt                                                                                                                   >  2:208517/1‑150 (MQ=255)
                                           cGAAAATTGTGGCGGGTGCTCGCTCTGGTGTGCCCGGACTTACGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGATGGGCTTTTCTTGCGAAATCAGTGGGAACCTGGAATAAAAg                                                                                                         <  2:1015648/150‑1 (MQ=255)
                                               aaTTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGt                                                                                                     <  1:746854/150‑1 (MQ=255)
                                                  tgtgGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGc                                                                                                  >  1:637728/1‑150 (MQ=255)
                                                   gtgGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGcc                                                                                                 >  2:607825/1‑150 (MQ=255)
                                                   gtgGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGcc                                                                                                 >  1:1072103/1‑150 (MQ=255)
                                                        ggTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGGAGt                                                                                            >  1:1256226/1‑150 (MQ=255)
                                                        ggTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGt                                                                                            >  2:1250164/1‑150 (MQ=255)
                                                        ggTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCAAGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGt                                                                                            >  1:799270/1‑150 (MQ=255)
                                                        ggTTGCTCGCTCTGGTGTGGTCGGACTTTAGCGCGCCGATAAAATAAAGCGTTGAGAATGATCTCAATGCGCAATTTACAGACCAACATGTAACGTAGGGCTTTTTTAGCGAAATCAGTGGTAACCTGGAATAAAAGCACTTGCCGCAGt                                                                                            >  1:1129571/1‑150 (MQ=255)
                                                          ttGCTCGCTCTGGTGGGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAATTCAGGGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTa                                                                                          >  1:49774/1‑150 (MQ=255)
                                                              tcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAAttt                                                                                      >  1:106661/1‑150 (MQ=255)
                                                                 ctctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCt                                                                                   >  1:1248662/1‑150 (MQ=255)
                                                                  tctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTg                                                                                  >  1:239677/1‑150 (MQ=255)
                                                                     ggTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTTGAATAAAAGCAGTTGCCGCAGTTAATTTTCTgagg                                                                               >  1:304446/1‑147 (MQ=255)
                                                                      gtgtGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCt                                                                              >  2:1311857/1‑150 (MQ=255)
                                                                             cGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGt                                                                       >  1:515167/1‑150 (MQ=255)
                                                                             cGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGt                                                                       >  1:808714/1‑150 (MQ=255)
                                                                              ggACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                                                                      >  2:430457/1‑150 (MQ=255)
                                                                              ggACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                                                                      >  1:1017184/1‑150 (MQ=255)
                                                                               gACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTa                                                                     >  1:1224232/1‑150 (MQ=255)
                                                                                 cTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTGGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGGTAATTTTCTGCTCTTATATGtatat                                                                   >  1:802935/1‑146 (MQ=255)
                                                                                         cggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTaa                                                           >  2:410081/1‑150 (MQ=255)
                                                                                         cggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTaa                                                           >  1:757415/1‑150 (MQ=255)
                                                                                          ggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGTGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAc                                                          <  2:56644/150‑1 (MQ=255)
                                                                                           gcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAcc                                                         <  1:376416/150‑1 (MQ=255)
                                                                                            cgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGTGCTTTTTTTGCGAAATCAGTGGGAACCTGGTATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTTGATGATAATGAATTTAAcac                                                        >  2:246298/1‑148 (MQ=255)
                                                                                            cgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCAGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAccc                                                        >  2:892262/1‑150 (MQ=255)
                                                                                            cgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGAAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGATTTTAAccc                                                        >  1:507957/1‑150 (MQ=255)
                                                                                             gATAAAATAATGCGGTGAGAATGATCTCAATGCGCAATTTCCAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCa                                                       <  2:625910/150‑1 (MQ=255)
                                                                                               tAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATa                                                     >  2:605095/1‑150 (MQ=255)
                                                                                                  aataatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCa                                                  <  1:230833/150‑1 (MQ=255)
                                                                                                     aatGCGTTGAGAATGATCTCAATGCGCAATATACAGCCCAACATGTCACGTTTGGCTTTTTTTGCGAAATCAGTGGGATCCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTAAGCGCTTAGATGTTAATGAATTTAACCCATACCAGTa                                               >  1:651547/1‑150 (MQ=255)
                                                                                                          gTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGAGAAATCAGTGGGAACCTGGAATAAAAGCAGATGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATg                                          >  1:921069/1‑150 (MQ=255)
                                                                                                               gaATGATCTCAATGCGCAATTTACAGCCAAGCATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGCGCACATGGATTAAAAGCAGTTGCCGCAGTTAATATTGAGCGCTTTGATGTTTATGATATTAACCCATACCAGTACATTGGCttt                                     >  2:90580/1‑148 (MQ=255)
                                                                                                                  tGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGt                                  <  1:1205877/150‑1 (MQ=255)
                                                                                                                   gATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGGTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCGTAGATGTTAATGAATTTAACCAATACCAGTACAATGGCTATGGtt                                 >  2:145647/1‑150 (MQ=255)
                                                                                                                   gATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTTGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGtt                                 <  2:339307/150‑1 (MQ=255)
                                                                                                                    aTCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGttt                                <  1:1092689/150‑1 (MQ=255)
                                                                                                                     tctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGtttt                               <  2:770085/150‑1 (MQ=255)
                                                                                                                      ctcAATGCGCAATTTACAGCCCAACATGTCAGGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCCTACCAGTACATTGGCTATGGttttt                              >  1:868450/1‑150 (MQ=255)
                                                                                                                       tcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTa                             <  1:1046931/150‑1 (MQ=255)
                                                                                                                        cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTAc                            >  2:1259210/1‑150 (MQ=255)
                                                                                                                        aaaTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTAc                            <  1:466423/149‑1 (MQ=255)
                                                                                                                          aTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACAt                          >  1:697910/1‑150 (MQ=255)
                                                                                                                                aaTTTACATCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACg                    <  2:987416/150‑1 (MQ=255)
                                                                                                                                aaTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACg                    <  2:632490/150‑1 (MQ=255)
                                                                                                                                   ttACATCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCaa                 <  1:1226941/150‑1 (MQ=255)
                                                                                                                                   ttACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTACATTTTACGCAAg                <  2:1305554/150‑1 (MQ=255)
                                                                                                                                      aaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAggg              >  1:1086816/2‑150 (MQ=255)
                                                                                                                                      aaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATAACAGTACAATGGCTATGGTTTTTACATTTTACGCAAggg              >  1:472850/2‑150 (MQ=255)
                                                                                                                                       aGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAgggg             >  1:766758/1‑150 (MQ=255)
                                                                                                                                               aTGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATtgtg     <  1:840832/150‑1 (MQ=255)
                                                                                                                                                tGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTTCGCTTAGATGATAATGAATTTAACCCATACCAGTACAAAGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGa    >  2:3520/1‑150 (MQ=255)
                                                                                                                                                 gTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGaa   >  2:733001/1‑150 (MQ=255)
                                                                                                                                                  tCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGaaa  >  1:889801/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGAAA  >  NC_000913/87738‑88033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: