Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,100,793 A→C 7.1% intergenic (+6/+58) ycdZ → / ← csgG DUF1097 family inner membrane protein/curli production assembly/transport outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,100,7930AC 7.1% 185.4 / 2.8 74intergenic (+6/+58)ycdZ/csgGDUF1097 family inner membrane protein/curli production assembly/transport outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (30/37);  new base C (4/2);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGT  >  NC_000913/1100648‑1100942
                                                                                                                                                 |                                                                                                                                                     
aGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGTGCCCCTCa                                                                                                                                                   <  2:346634/150‑1 (MQ=255)
aGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCa                                                                                                                                                   >  1:981950/1‑150 (MQ=255)
   gTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGc                                                                                                                                                <  2:459363/150‑1 (MQ=255)
    tGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCt                                                                                                                                               <  1:1228322/150‑1 (MQ=255)
    tGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCGGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCt                                                                                                                                               <  1:645957/150‑1 (MQ=255)
    tGACTGGAAACTGGTGTTACCTTAGCTGTCGATTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCt                                                                                                                                               <  2:893091/150‑1 (MQ=255)
      aCTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCttt                                                                                                                                             >  2:351127/1‑150 (MQ=255)
      aCTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGAAGTGCAAAGACGGCGCCCCGAGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCttt                                                                                                                                             >  2:73088/1‑150 (MQ=255)
        tGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATTAAAAACAGTGGCCTGTGGCTGGCTGCTCGTATTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGTGCCCCTCACGCtttat                                                                                                                                           <  2:28389/150‑3 (MQ=255)
         ggacccTGGTGTTACCTTCGCTGGCGCGTGCGCGGAGAGTTGGTTCCGCACTGAAACACCGTGGCCTGTGGCTGGCGGCGCGTAGAGCAAAGCCGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGc                                                                                                                                          <  1:664946/145‑1 (MQ=255)
         ggAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGc                                                                                                                                          >  1:737823/1‑150 (MQ=255)
          gAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCt                                                                                                                                         <  1:736041/150‑1 (MQ=255)
           aaaCTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCtt                                                                                                                                        <  2:1290373/150‑1 (MQ=255)
           aaaCTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGGGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCtt                                                                                                                                        <  2:67736/150‑1 (MQ=255)
           aaaCTGGCGTTACCGTCGCTGGCGCCTCGGCTGATATATGGTTACGCACTGAAAAACAGTGGCCCGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCtt                                                                                                                                        <  1:207879/150‑1 (MQ=255)
                   gtTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTa                                                                                                                                >  1:827533/1‑150 (MQ=255)
                     tACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTaaa                                                                                                                              >  1:226616/1‑150 (MQ=255)
                       ccTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGAGCAAAGACGGCGCACCGTGCGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAAt                                                                                                                            >  2:73874/1‑150 (MQ=255)
                             cTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGga                                                                                                                      >  1:1080800/1‑150 (MQ=255)
                              tGGCGCTTGGGCTGATATTTGGTTACGCATTGAAATACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGgat                                                                                                                     <  2:1038200/150‑1 (MQ=255)
                              tGGCGCTTGGGCTGATATTTGGTGACGCAATGAAAAACAGTGGCCTGAGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGgat                                                                                                                     <  2:1032770/150‑1 (MQ=255)
                               ggCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCGGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGgatg                                                                                                                    <  1:92013/150‑1 (MQ=255)
                                gcgcTGGGGCTGATATTTGGTTACGCAATGACAAACCGGGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAACTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGgatga                                                                                                                   <  2:933930/150‑1 (MQ=255)
                                 cgcTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGgatgat                                                                                                                  <  1:1047665/150‑1 (MQ=255)
                                  gtttGGTCTGATATTTTGTTACGCAATGATAATCAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATa                                                                                                                 <  2:1273025/148‑1 (MQ=255)
                                   cTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATaa                                                                                                                >  2:690349/1‑150 (MQ=255)
                                    ttGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAAt                                                                                                               <  1:821403/150‑1 (MQ=255)
                                        gCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGAGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCg                                                                                                           >  2:293357/1‑150 (MQ=255)
                                         cTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCgg                                                                                                          >  1:142960/1‑150 (MQ=255)
                                         cTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGAGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCgg                                                                                                          <  1:32562/150‑1 (MQ=255)
                                          tGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGTCGCGTAGTGCAAAGACGGCGCACCGTCAGCAGGAAAAAAAAAATAAAGCGTGAGTGGCCCTCACGCTTACGCTTAAACAGTATAATGACGGATGAAAATTCCGGc                                                                                                         >  2:742937/1‑150 (MQ=255)
                                          tGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGATATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGc                                                                                                         <  2:129156/150‑1 (MQ=255)
                                                  ggTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTAt                                                                                                 <  2:3782/150‑1 (MQ=255)
                                                      aCGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGt                                                                                             >  2:259192/1‑150 (MQ=255)
                                                         cAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAg                                                                                          >  2:413764/1‑150 (MQ=255)
                                                         cAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAg                                                                                          >  2:236226/1‑150 (MQ=255)
                                                          aaTGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCTCCGTTAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAgg                                                                                         <  1:164094/150‑1 (MQ=255)
                                                              aaaaaCAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAACACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACTCTTTCGCGTTAACAGTAAAAAGCCGGATGATTAATCCGGCTGTTTTATCTGTAAGGATTc                                                                                     >  2:1129491/1‑150 (MQ=255)
                                                                           tgtgGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGAc                                                                        <  2:682525/150‑1 (MQ=255)
                                                                                  ggcggcGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAACTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGc                                                                 <  2:643854/150‑1 (MQ=255)
                                                                                  ggcggcGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGc                                                                 >  2:966889/1‑150 (MQ=255)
                                                                                  ggcggcGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGc                                                                 <  2:776147/150‑1 (MQ=255)
                                                                                      gcgcGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTa                                                             <  1:184622/150‑1 (MQ=255)
                                                                                       cgcgTAGTGTAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTAt                                                            >  2:788355/1‑150 (MQ=255)
                                                                                       cgcgTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTAt                                                            >  1:570230/1‑150 (MQ=255)
                                                                                        gcgTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCTCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTAtt                                                           <  2:725435/150‑1 (MQ=255)
                                                                                             gTGCAAAGACGGCGCCCCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCAcc                                                      >  2:745183/1‑150 (MQ=255)
                                                                                             gTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCAcc                                                      >  2:658380/1‑150 (MQ=255)
                                                                                              ggCAAAGACGGCGCACCGTGAGCAGGATATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAAAGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCa                                                     <  2:689507/149‑1 (MQ=255)
                                                                                                cAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGa                                                   <  1:662380/150‑1 (MQ=255)
                                                                                                 aaaGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAgta                                                  >  2:242634/1‑148 (MQ=255)
                                                                                                       ggagcACCGGGAGCAGGAAATCAAAAATCAATCGTGATGGTCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATACTTCCGTCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCAt                                            <  1:73874/147‑1 (MQ=255)
                                                                                                        gcgcACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCAtt                                           <  2:155717/150‑1 (MQ=255)
                                                                                                         cgcACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTc                                          <  2:507096/150‑1 (MQ=255)
                                                                                                                 gAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTc                                  <  2:993340/150‑1 (MQ=255)
                                                                                                                 gAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTc                                  >  2:1019235/1‑150 (MQ=255)
                                                                                                                  aGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTC‑‑ACTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGc                               >  1:315889/1‑150 (MQ=255)
                                                                                                                      ggAAATAAAAAATAATGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATTATATTTCCTGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATTTGGCGGTATTTCTCCAGAATGTCATTCTGCCGTTCTGCtt                             <  1:858805/150‑1 (MQ=255)
                                                                                                                        aaaTCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTa                           >  2:996466/1‑150 (MQ=255)
                                                                                                                          aTCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTAtt                         >  2:866909/1‑150 (MQ=255)
                                                                                                                              aaaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGc                     <  2:836539/150‑1 (MQ=255)
                                                                                                                              aaaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGc                     <  1:124409/150‑1 (MQ=255)
                                                                                                                               aaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGCCATATGGCGGTATTTCACAAGAATGTCATTCTGCCGTTCTGCTTTATATTGCa                    >  1:856677/1‑150 (MQ=255)
                                                                                                                               aaaTAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCa                    <  2:643110/150‑1 (MQ=255)
                                                                                                                                 cgaaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACAGATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCaaa                  >  1:996912/3‑150 (MQ=255)
                                                                                                                                  gaaaGCGTGAGTGCCCCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAAt                 >  2:1022005/2‑150 (MQ=255)
                                                                                                                                     aGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATccc              >  2:1064661/1‑150 (MQ=255)
                                                                                                                                        gTGAGGGGCACTCACTCTTTCGCTTAAACAGTAAAATGCCGGATGATAATGCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCcaca           <  1:68712/150‑1 (MQ=255)
                                                                                                                                           aGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAc        <  2:827533/150‑1 (MQ=255)
                                                                                                                                            ggggCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTTATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGAcc       >  1:112162/1‑150 (MQ=255)
                                                                                                                                              ggCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGGCATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCAc     >  1:557834/1‑150 (MQ=255)
                                                                                                                                              ggCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCAc     >  1:661508/1‑150 (MQ=255)
                                                                                                                                              ggCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCAc     >  1:703891/1‑150 (MQ=255)
                                                                                                                                               gCACTCACGCTTTAGCTTAACCAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGAATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACg    <  1:225020/150‑1 (MQ=255)
                                                                                                                                                 aCTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGt  <  2:440572/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
AGGGTGACTGGAAACTGGTGTTACCTTCGCTGGCGCTTGGGCTGATATTTGGTTACGCAATGAAAAACAGTGGCCTGTGGCTGGCGGCGCGTAGTGCAAAGACGGCGCACCGTGAGCAGGAAATCAAAAATAAAGCGTGAGGGGCACTCACGCTTTCGCTTAAACAGTAAAATGCCGGATGATAATTCCGGCTTTTTTATCTGTCAGGATTCCGGTGGAACCGACATATGGCGGTATTTCACCAGAATGTCATTCTGCCGTTCTGCTTTATTTTGCAAATCCCACAGACCACGGT  >  NC_000913/1100648‑1100942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: