Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,181,765 C→T 6.6% intergenic (‑40/+18) ymfA ← / ← potD DUF3592 family inner membrane protein/spermidine/putrescine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,181,7650CT 6.6% 171.0 / 2.1 64intergenic (‑40/+18)ymfA/potDDUF3592 family inner membrane protein/spermidine/putrescine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (25/32);  new base T (2/2);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.96e-01

GTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACTTCTGG  >  NC_000913/1181618‑1181914
                                                                                                                                                   |                                                                                                                                                     
gTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATaccac                                                                                                                                                     <  1:1059112/150‑1 (MQ=255)
gTCTTTGCTATAGGTGAACTGGGTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATaccac                                                                                                                                                     <  1:1064468/150‑1 (MQ=255)
 tCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAACCGGGCGGTAAAaccacc                                                                                                                                                    >  1:975287/1‑150 (MQ=255)
 tCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATaccacc                                                                                                                                                    <  1:111272/150‑1 (MQ=255)
 tCTATGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATaccacc                                                                                                                                                    <  2:425380/150‑1 (MQ=255)
    ttGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgcc                                                                                                                                                 <  2:603668/150‑1 (MQ=255)
    ttGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgcc                                                                                                                                                 >  1:1184160/1‑150 (MQ=255)
    ttGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACAAccgcc                                                                                                                                                 >  1:308836/1‑150 (MQ=255)
     tGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACAGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgccc                                                                                                                                                <  2:198317/150‑1 (MQ=255)
       cTATAGGTGAATTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgcccgc                                                                                                                                              >  2:1128555/1‑150 (MQ=255)
       cTATAGGTGAACGGGTAATAAAAGACAACAACATAAATGAGGATCAGTACCAGACCGATACCGAGGAATAAACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTGGTATTAccgcccgt                                                                                                                                              <  1:958275/150‑2 (MQ=255)
          taGGGGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTg                                                                                                                                           <  1:136241/150‑1 (MQ=255)
           aGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGc                                                                                                                                          <  2:961552/150‑1 (MQ=255)
           aGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGACGAATATACGAAAGAAAACTTTACTGGCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGc                                                                                                                                          <  1:56359/150‑1 (MQ=255)
            ggTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAGAAACGGGCGGTGGTATTACCGCCCGtttttt                                                                                                                                         <  1:339921/150‑4 (MQ=255)
             gTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTg                                                                                                                                        <  1:552398/150‑1 (MQ=255)
               gAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGaa                                                                                                                                      >  2:5854/1‑150 (MQ=255)
                     gTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACg                                                                                                                                <  2:1133924/150‑1 (MQ=255)
                      ttATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGt                                                                                                                               <  1:295435/150‑1 (MQ=255)
                       tataAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTc                                                                                                                              >  2:323888/1‑150 (MQ=255)
                        ataAAAGACAACAACAGAAATGAGGATCAGTACCAGACCTATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTcc                                                                                                                             <  1:956627/150‑1 (MQ=255)
                        ataAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTACCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGAcc                                                                                                                             <  1:956630/150‑1 (MQ=255)
                         taAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCt                                                                                                                            >  2:70678/1‑150 (MQ=255)
                               acaacaacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCTCCGCCCGCTTGCTGAATTAACGTCCTGCTTTc                                                                                                                      <  1:1195050/150‑1 (MQ=255)
                                caacaacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCa                                                                                                                     <  1:570818/150‑1 (MQ=255)
                                 aacaacaGAAATTAGTATCAGTACCAGACCTATACCGAGGAATATACGAAAGTAAACTTTACTGTCCTTAATCCTTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAg                                                                                                                    <  2:362251/150‑1 (MQ=255)
                                  acaacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGc                                                                                                                   <  1:1061712/150‑1 (MQ=255)
                                         aaaTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGat                                                                                                            <  2:1157737/150‑1 (MQ=255)
                                         aaaTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGat                                                                                                            <  2:1231064/150‑1 (MQ=255)
                                         aaaTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGat                                                                                                            <  2:1162932/150‑1 (MQ=255)
                                             taGGATCAGTACCAGACCTATTCCGAGGAATATACGAAAGTAAACTTAACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACTTGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGataata                                                                                                        <  1:78696/149‑1 (MQ=255)
                                                gATCAGTACCAGACCGATACAGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCCTGCTAACTAAAAAACGGGCGTTAATAACAACGCCCGCTTGCTCAATAAACGTCATACTTACAGCTTCGGAAAATActg                                                                                                     >  2:819184/1‑149 (MQ=255)
                                                  tCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCtt                                                                                                   >  1:240146/1‑150 (MQ=255)
                                                  tCAGTACCAGACCGATACCGAGGAATAAACTAAAAAAAACTTTAAAGTAATCACTCATTAACCACATCCTAGATAACTAAAAAACGGGCGGAAAAACAACCGCCCGCTTGCTGAATTAACGTACAGATATCACCATCTGATAATAcacat                                                                                                   >  2:285760/1‑146 (MQ=255)
                                                   cAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTc                                                                                                  >  2:944652/1‑150 (MQ=255)
                                                    aGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCa                                                                                                 <  1:377820/150‑1 (MQ=255)
                                                          aGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAtg                                                                                           <  1:167460/150‑1 (MQ=255)
                                                                     gAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTgc                                                                                >  1:544078/1‑150 (MQ=255)
                                                                         aaTATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCTCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCGTCTGATAATTCTCTTCATAAATGCTGCAGGCTGCGccc                                                                            >  2:857539/1‑149 (MQ=255)
                                                                            ataCGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACg                                                                         <  2:929846/150‑1 (MQ=255)
                                                                             taCGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGt                                                                        >  1:1057936/1‑150 (MQ=255)
                                                                                gaaagaaaACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCAAACGTCAt                                                                     <  2:1132971/150‑1 (MQ=255)
                                                                                    gaaaACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAAATAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTg                                                                 >  1:941085/1‑150 (MQ=255)
                                                                                             aCTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGcc                                                        <  2:934257/150‑1 (MQ=255)
                                                                                             aCTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGcc                                                        <  2:134712/150‑1 (MQ=255)
                                                                                             aCTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGcc                                                        <  2:31732/150‑1 (MQ=255)
                                                                                                  ccTGACTCATTAGACACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGATTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCTTTCTGCAATTCGAAAtttt                                                   >  2:818207/1‑150 (MQ=255)
                                                                                                    tGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTa                                                 >  1:609177/1‑150 (MQ=255)
                                                                                                       cTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATg                                              <  2:478618/150‑1 (MQ=255)
                                                                                                          aTTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGtt                                           >  2:718995/1‑150 (MQ=255)
                                                                                                          aTTAGCCACATCCTAGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTTCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGtt                                           <  1:962616/150‑1 (MQ=255)
                                                                                                              gCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCAGTTTTAATGGTTTCAg                                       >  2:675089/1‑150 (MQ=255)
                                                                                                               ccACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGc                                      <  1:121176/150‑1 (MQ=255)
                                                                                                                cacaTCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCa                                     >  1:924551/1‑150 (MQ=255)
                                                                                                                  caTCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATc                                   >  1:582445/1‑150 (MQ=255)
                                                                                                                      cTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCggg                               >  1:358091/1‑150 (MQ=255)
                                                                                                                      cTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCggg                               >  1:353079/1‑150 (MQ=255)
                                                                                                                            aactaaAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAgt                         <  1:323888/150‑1 (MQ=255)
                                                                                                                              ctaaAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAAGTAACGTCCTGCTTTCAGCTTCTGCTAATACTCTTCATAAGTGCTGCTGGCTGCGCCAACGTCATTCTGCCAATCGCCATTTTTTATGTTTTCAGCTTCCGGGTAGAgagt                       >  2:792349/1‑150 (MQ=255)
                                                                                                                                   aaaCGGGAGGTAATACGACCGCCCGCTTGCTGAATTACCGTCCTGCATTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTAAt                  <  1:500292/150‑1 (MQ=255)
                                                                                                                                     gcGGGCGGTGGTATTACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATGCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCg                >  2:95065/2‑150 (MQ=255)
                                                                                                                                      cGGGCGGTGGTATTACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGt               >  2:727260/1‑150 (MQ=255)
                                                                                                                                      cGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGTGTGTTTTATCGt               <  1:383830/150‑1 (MQ=255)
                                                                                                                                      cGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGt               <  1:423138/150‑1 (MQ=255)
                                                                                                                                       gggCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATAGTCTTCATAAATGCTTCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTATTGGTTTCAGCATCCGGGTAGAGTGTTTTATCGtt              >  2:650873/1‑150 (MQ=255)
                                                                                                                                       gggCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGtt              >  1:1696/1‑150 (MQ=255)
                                                                                                                                           ggTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGcc          >  1:135325/1‑150 (MQ=255)
                                                                                                                                              aaTACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACt       <  2:66287/150‑1 (MQ=255)
                                                                                                                                              aaTACCACCGCCCGCTTGCTGAATTAACGTCCTGCTATCAGCTTCTGATAATACTCTACATAAATGCTGCTGGCTGCGCCAACGACATACTGCCAATCCCCAGTTTTAATGGATTACGCATCCGGGTAGAGTGTTTTATCGTTCGCCACt       >  1:1203899/1‑150 (MQ=255)
                                                                                                                                                   caccGCGCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACTTCTgg  <  2:1219303/150‑1 (MQ=255)
                                                                                                                                                   caccGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACTTCTgg  <  1:379210/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACTTCTGG  >  NC_000913/1181618‑1181914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: